Structural Diversity in the Plasmodium falciparum Merozoite Surface Antigen 2
Antigens associated with the surface of merozoites of the malaria parasite Plasmodium falciparum are directly accessible to immune attack and therefore are prime vaccine candidates. We have previously shown that one of the two known merozoite surface antigens (merozoite surface antigen 2; MSA-2) exh...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-03, Vol.88 (5), p.1751-1755 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1755 |
|---|---|
| container_issue | 5 |
| container_start_page | 1751 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 88 |
| creator | Smythe, Jason A. Coppel, Ross L. Day, Karen P. Martin, Rodger K. Ayoade M. J. Oduola Kemp, David J. Anders, Robin F. |
| description | Antigens associated with the surface of merozoites of the malaria parasite Plasmodium falciparum are directly accessible to immune attack and therefore are prime vaccine candidates. We have previously shown that one of the two known merozoite surface antigens (merozoite surface antigen 2; MSA-2) exhibits considerable sequence and antigenic diversity in different isolates. The sequences of MSA-2 from three isolates revealed a central domain composed of repeats that vary in number, length, and sequence, flanked in turn by nonrepetitive variable sequences and by conserved N- and C-terminal domains. We report here the sequences of a further four MSA-2 alleles, containing repetitive sequences that are related but not identical to each other. The seven alleles of MSA-2 can be divided into two distinct allele families on the basis of nonrepetitive sequences. Hybridization studies with repeat probes indicated that all of the 44 P. falciparum isolates examined contained repeat regions similar to those defined in known MSA-2 sequences. |
| doi_str_mv | 10.1073/pnas.88.5.1751 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_jstor_primary_2356593</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>2356593</jstor_id><sourcerecordid>2356593</sourcerecordid><originalsourceid>FETCH-LOGICAL-c518t-b690dca27ecb086d9a62b0657fb9cee58ce0206b8a613517a395f0e941eefa4d3</originalsourceid><addsrcrecordid>eNqFUUtrFTEUDqLUa3XrSmE2djfjSTLJJOCm1PqAFoXqOmQyZ9qUeVyTTLH-enO519srCK5y4Huc7-Qj5CWFikLD364nGyulKlHRRtBHZEVB01LWGh6TFQBrSlWz-il5FuMtAGih4IgcsTxyxVfk8iqFxaUl2KF47-8wRJ_uCz8V6QaLr4ON49z5ZSx6Ozi_tiGPlxjmX7NPWFwtobcOi9Mp-WucCvacPMnEiC927zH5_uH829mn8uLLx89npxelE1SlspUaOmdZg64FJTttJWtBiqZvtUMUyiEwkK2yknJBG8u16AF1TRF7W3f8mLzb-q6XdsTO4ZTyAWYd_GjDvZmtN38jk78x1_OdEZQCy_KTnTzMPxaMyYw-OhwGO-G8RKOglpxT-V8ilflH60ZnYrUlujDHGLDfZ6FgNj2ZTU9GKSPMpqcseH14wZ6-Kybjb3a4jc4OfbCT8_HBVSvKlBYHCTf-f-D9HtMvw5DwZzpY-E9ixl9t8duY5vCQhwspNOe_AaOevkM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16009479</pqid></control><display><type>article</type><title>Structural Diversity in the Plasmodium falciparum Merozoite Surface Antigen 2</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Smythe, Jason A. ; Coppel, Ross L. ; Day, Karen P. ; Martin, Rodger K. ; Ayoade M. J. Oduola ; Kemp, David J. ; Anders, Robin F.</creator><creatorcontrib>Smythe, Jason A. ; Coppel, Ross L. ; Day, Karen P. ; Martin, Rodger K. ; Ayoade M. J. Oduola ; Kemp, David J. ; Anders, Robin F.</creatorcontrib><description>Antigens associated with the surface of merozoites of the malaria parasite Plasmodium falciparum are directly accessible to immune attack and therefore are prime vaccine candidates. We have previously shown that one of the two known merozoite surface antigens (merozoite surface antigen 2; MSA-2) exhibits considerable sequence and antigenic diversity in different isolates. The sequences of MSA-2 from three isolates revealed a central domain composed of repeats that vary in number, length, and sequence, flanked in turn by nonrepetitive variable sequences and by conserved N- and C-terminal domains. We report here the sequences of a further four MSA-2 alleles, containing repetitive sequences that are related but not identical to each other. The seven alleles of MSA-2 can be divided into two distinct allele families on the basis of nonrepetitive sequences. Hybridization studies with repeat probes indicated that all of the 44 P. falciparum isolates examined contained repeat regions similar to those defined in known MSA-2 sequences.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.5.1751</identifier><identifier>PMID: 2000383</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Alleles ; Amino Acid Sequence ; Amino acids ; Animals ; antigen 2 ; Antigens ; Antigens, Protozoan - genetics ; Antigens, Surface - genetics ; Base Sequence ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; cell surface ; Chemical bases ; DNA probes ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic mutation ; Genetic Variation ; Invertebrates ; Merozoites ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Nucleotides ; Oligonucleotide Probes ; Parasites ; Plasmodium falciparum - genetics ; Plasmodium falciparum - immunology ; Polymerase Chain Reaction ; Protozoa ; Sequence Homology, Nucleic Acid</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-03, Vol.88 (5), p.1751-1755</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-b690dca27ecb086d9a62b0657fb9cee58ce0206b8a613517a395f0e941eefa4d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2356593$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2356593$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19812895$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2000383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smythe, Jason A.</creatorcontrib><creatorcontrib>Coppel, Ross L.</creatorcontrib><creatorcontrib>Day, Karen P.</creatorcontrib><creatorcontrib>Martin, Rodger K.</creatorcontrib><creatorcontrib>Ayoade M. J. Oduola</creatorcontrib><creatorcontrib>Kemp, David J.</creatorcontrib><creatorcontrib>Anders, Robin F.</creatorcontrib><title>Structural Diversity in the Plasmodium falciparum Merozoite Surface Antigen 2</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Antigens associated with the surface of merozoites of the malaria parasite Plasmodium falciparum are directly accessible to immune attack and therefore are prime vaccine candidates. We have previously shown that one of the two known merozoite surface antigens (merozoite surface antigen 2; MSA-2) exhibits considerable sequence and antigenic diversity in different isolates. The sequences of MSA-2 from three isolates revealed a central domain composed of repeats that vary in number, length, and sequence, flanked in turn by nonrepetitive variable sequences and by conserved N- and C-terminal domains. We report here the sequences of a further four MSA-2 alleles, containing repetitive sequences that are related but not identical to each other. The seven alleles of MSA-2 can be divided into two distinct allele families on the basis of nonrepetitive sequences. Hybridization studies with repeat probes indicated that all of the 44 P. falciparum isolates examined contained repeat regions similar to those defined in known MSA-2 sequences.</description><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>antigen 2</subject><subject>Antigens</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Surface - genetics</subject><subject>Base Sequence</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>cell surface</subject><subject>Chemical bases</subject><subject>DNA probes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic mutation</subject><subject>Genetic Variation</subject><subject>Invertebrates</subject><subject>Merozoites</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Nucleotides</subject><subject>Oligonucleotide Probes</subject><subject>Parasites</subject><subject>Plasmodium falciparum - genetics</subject><subject>Plasmodium falciparum - immunology</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoa</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtrFTEUDqLUa3XrSmE2djfjSTLJJOCm1PqAFoXqOmQyZ9qUeVyTTLH-enO519srCK5y4Huc7-Qj5CWFikLD364nGyulKlHRRtBHZEVB01LWGh6TFQBrSlWz-il5FuMtAGih4IgcsTxyxVfk8iqFxaUl2KF47-8wRJ_uCz8V6QaLr4ON49z5ZSx6Ozi_tiGPlxjmX7NPWFwtobcOi9Mp-WucCvacPMnEiC927zH5_uH829mn8uLLx89npxelE1SlspUaOmdZg64FJTttJWtBiqZvtUMUyiEwkK2yknJBG8u16AF1TRF7W3f8mLzb-q6XdsTO4ZTyAWYd_GjDvZmtN38jk78x1_OdEZQCy_KTnTzMPxaMyYw-OhwGO-G8RKOglpxT-V8ilflH60ZnYrUlujDHGLDfZ6FgNj2ZTU9GKSPMpqcseH14wZ6-Kybjb3a4jc4OfbCT8_HBVSvKlBYHCTf-f-D9HtMvw5DwZzpY-E9ixl9t8duY5vCQhwspNOe_AaOevkM</recordid><startdate>19910301</startdate><enddate>19910301</enddate><creator>Smythe, Jason A.</creator><creator>Coppel, Ross L.</creator><creator>Day, Karen P.</creator><creator>Martin, Rodger K.</creator><creator>Ayoade M. J. Oduola</creator><creator>Kemp, David J.</creator><creator>Anders, Robin F.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910301</creationdate><title>Structural Diversity in the Plasmodium falciparum Merozoite Surface Antigen 2</title><author>Smythe, Jason A. ; Coppel, Ross L. ; Day, Karen P. ; Martin, Rodger K. ; Ayoade M. J. Oduola ; Kemp, David J. ; Anders, Robin F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-b690dca27ecb086d9a62b0657fb9cee58ce0206b8a613517a395f0e941eefa4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>antigen 2</topic><topic>Antigens</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Surface - genetics</topic><topic>Base Sequence</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>cell surface</topic><topic>Chemical bases</topic><topic>DNA probes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic mutation</topic><topic>Genetic Variation</topic><topic>Invertebrates</topic><topic>Merozoites</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Nucleotides</topic><topic>Oligonucleotide Probes</topic><topic>Parasites</topic><topic>Plasmodium falciparum - genetics</topic><topic>Plasmodium falciparum - immunology</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoa</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smythe, Jason A.</creatorcontrib><creatorcontrib>Coppel, Ross L.</creatorcontrib><creatorcontrib>Day, Karen P.</creatorcontrib><creatorcontrib>Martin, Rodger K.</creatorcontrib><creatorcontrib>Ayoade M. J. Oduola</creatorcontrib><creatorcontrib>Kemp, David J.</creatorcontrib><creatorcontrib>Anders, Robin F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smythe, Jason A.</au><au>Coppel, Ross L.</au><au>Day, Karen P.</au><au>Martin, Rodger K.</au><au>Ayoade M. J. Oduola</au><au>Kemp, David J.</au><au>Anders, Robin F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural Diversity in the Plasmodium falciparum Merozoite Surface Antigen 2</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-03-01</date><risdate>1991</risdate><volume>88</volume><issue>5</issue><spage>1751</spage><epage>1755</epage><pages>1751-1755</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Antigens associated with the surface of merozoites of the malaria parasite Plasmodium falciparum are directly accessible to immune attack and therefore are prime vaccine candidates. We have previously shown that one of the two known merozoite surface antigens (merozoite surface antigen 2; MSA-2) exhibits considerable sequence and antigenic diversity in different isolates. The sequences of MSA-2 from three isolates revealed a central domain composed of repeats that vary in number, length, and sequence, flanked in turn by nonrepetitive variable sequences and by conserved N- and C-terminal domains. We report here the sequences of a further four MSA-2 alleles, containing repetitive sequences that are related but not identical to each other. The seven alleles of MSA-2 can be divided into two distinct allele families on the basis of nonrepetitive sequences. Hybridization studies with repeat probes indicated that all of the 44 P. falciparum isolates examined contained repeat regions similar to those defined in known MSA-2 sequences.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2000383</pmid><doi>10.1073/pnas.88.5.1751</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-03, Vol.88 (5), p.1751-1755 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_2356593 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Alleles Amino Acid Sequence Amino acids Animals antigen 2 Antigens Antigens, Protozoan - genetics Antigens, Surface - genetics Base Sequence Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences cell surface Chemical bases DNA probes Fundamental and applied biological sciences. Psychology Genes Genetic mutation Genetic Variation Invertebrates Merozoites Molecular Sequence Data Nucleic Acid Hybridization Nucleotides Oligonucleotide Probes Parasites Plasmodium falciparum - genetics Plasmodium falciparum - immunology Polymerase Chain Reaction Protozoa Sequence Homology, Nucleic Acid |
| title | Structural Diversity in the Plasmodium falciparum Merozoite Surface Antigen 2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T19%3A54%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Structural%20Diversity%20in%20the%20Plasmodium%20falciparum%20Merozoite%20Surface%20Antigen%202&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Smythe,%20Jason%20A.&rft.date=1991-03-01&rft.volume=88&rft.issue=5&rft.spage=1751&rft.epage=1755&rft.pages=1751-1755&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.88.5.1751&rft_dat=%3Cjstor_proqu%3E2356593%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16009479&rft_id=info:pmid/2000383&rft_jstor_id=2356593&rfr_iscdi=true |