Ionic strength-dependent persistence lengths of single-stranded RNA and DNA
Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understa...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2012-01, Vol.109 (3), p.799-804 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Chen, Huimin Meisburger, Steve P Pabit, Suzette A Sutton, Julie L Webb, Watt W Pollack, Lois |
| description | Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understanding of RNA folding and dynamics it is critical to know the flexibility of these un-base-paired regions and how it depends on counterions. Yet, information about nucleic acid polymer properties is mainly derived from studies of ssDNA. Here we measure the persistence lengths (lp) of ssRNA. We observe valence and ionic strength-dependent differences in lp in a direct comparison between 40-mers of deoxythymidylate (dT40) and uridylate (rU40) measured using the powerful combination of SAXS and smFRET. We also show that nucleic acid flexibility is influenced by local environment (an adjoining double helix). Our results illustrate the complex interplay between conformation and ion environment that modulates nucleic acid function in vivo. |
| doi_str_mv | 10.1073/pnas.1119057109 |
| format | Article |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2012-01, Vol.109 (3), p.799-804 |
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| subjects | Biological Sciences Chemical bases Deoxyribonucleic acid DNA DNA, Single-Stranded - chemistry Electrostatics Fluorescence Fluorescence Resonance Energy Transfer Ions Magnesium Chloride - pharmacology Models, Molecular Molecules Nucleic acids Osmolar Concentration Pliability - drug effects Polymers Ribonucleic acid RNA RNA - chemistry Salts Scattering, Small Angle single-stranded DNA Sodium Chloride - pharmacology translation (genetics) X-Ray Diffraction |
| title | Ionic strength-dependent persistence lengths of single-stranded RNA and DNA |
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