Localization of a Poliovirus Type 1 Neutralization Epitope in Viral Capsid Polypeptide VP1
Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the β -lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-β -lactamase fusion protein that reacted with antibodies raised against p...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1983-08, Vol.80 (16), p.5080-5084 |
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| container_issue | 16 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Van Der Werf, Sylvie Wychowski, Czeslaw Bruneau, Patrick Blondel, Bruno Crainic, Radu Horodniceanu, Florian Girard, Marc |
| description | Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the β -lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-β -lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide. |
| doi_str_mv | 10.1073/pnas.80.16.5080 |
| format | Article |
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Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-β -lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.80.16.5080</identifier><identifier>PMID: 6308670</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Antibodies ; Antibodies, Monoclonal ; Antigen-Antibody Complex ; Base Sequence ; Capsid ; DNA ; DNA - analysis ; DNA Restriction Enzymes ; Epitopes ; Escherichia coli - genetics ; Mice ; Monoclonal antibodies ; Nucleotides ; Plasmids ; Poliovirus ; Poliovirus - genetics ; poliovirus 1 ; Viral Proteins - genetics ; Viral Proteins - immunology ; Viral Structural Proteins ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1983-08, Vol.80 (16), p.5080-5084</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-8cdfa071ea0e06c2c7a9ba9ae728b2ea02a8dd6d87f2f7613a02b3df8bf3526f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/80/16.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/14195$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/14195$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6308670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Der Werf, Sylvie</creatorcontrib><creatorcontrib>Wychowski, Czeslaw</creatorcontrib><creatorcontrib>Bruneau, Patrick</creatorcontrib><creatorcontrib>Blondel, Bruno</creatorcontrib><creatorcontrib>Crainic, Radu</creatorcontrib><creatorcontrib>Horodniceanu, Florian</creatorcontrib><creatorcontrib>Girard, Marc</creatorcontrib><title>Localization of a Poliovirus Type 1 Neutralization Epitope in Viral Capsid Polypeptide VP1</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the β -lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-β -lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Antigen-Antibody Complex</subject><subject>Base Sequence</subject><subject>Capsid</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA Restriction Enzymes</subject><subject>Epitopes</subject><subject>Escherichia coli - genetics</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>Nucleotides</subject><subject>Plasmids</subject><subject>Poliovirus</subject><subject>Poliovirus - genetics</subject><subject>poliovirus 1</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><subject>Viral Structural Proteins</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbFP3DAYxS0EoldgrlQJ5KlMOT7bF9sZOlQnSpFO5QZgYLGcxC5GuTjEDir96-vorgcsdLL8vd_79OyH0CcCUwKCnXWtDlOZLnyag4QdNCFQkIzPCthFEwAqMjmjsw_oYwgPAFDkEvbRPmcguYAJulv4Sjfuj47Ot9hbrPHSN84_uX4I-Pq5M5jgn2aI_Qt13rnok-BafOvSHM91F1w9GhPfRVcbfLskh2jP6iaYo815gG6-n1_Pf2SLq4vL-bdFVuW5jJmsaqtBEKPBAK9oJXRR6kIbQWVJ05RqWde8lsJSKzhhaVKy2srSspxyyw7Q1_XebihXpq5MO4ZVXe9Wun9WXjv1VmndvfrlnxSTM1LQ5P-y8ff-cTAhqpULlWka3Ro_BCUhl4KI_L8gYZwXnIzg2Rqseh9Cb-w2DAE11qbG2tJiRbgaa0uO49dv2PKbnpJ-stFH4z_1zYLTdwFlh6aJ5ndM5Oc1-RCi71-Spb_I2V8k_7az</recordid><startdate>19830801</startdate><enddate>19830801</enddate><creator>Van Der Werf, Sylvie</creator><creator>Wychowski, Czeslaw</creator><creator>Bruneau, Patrick</creator><creator>Blondel, Bruno</creator><creator>Crainic, Radu</creator><creator>Horodniceanu, Florian</creator><creator>Girard, Marc</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19830801</creationdate><title>Localization of a Poliovirus Type 1 Neutralization Epitope in Viral Capsid Polypeptide VP1</title><author>Van Der Werf, Sylvie ; Wychowski, Czeslaw ; Bruneau, Patrick ; Blondel, Bruno ; Crainic, Radu ; Horodniceanu, Florian ; Girard, Marc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-8cdfa071ea0e06c2c7a9ba9ae728b2ea02a8dd6d87f2f7613a02b3df8bf3526f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Antigen-Antibody Complex</topic><topic>Base Sequence</topic><topic>Capsid</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA Restriction Enzymes</topic><topic>Epitopes</topic><topic>Escherichia coli - genetics</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>Nucleotides</topic><topic>Plasmids</topic><topic>Poliovirus</topic><topic>Poliovirus - genetics</topic><topic>poliovirus 1</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - immunology</topic><topic>Viral Structural Proteins</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Der Werf, Sylvie</creatorcontrib><creatorcontrib>Wychowski, Czeslaw</creatorcontrib><creatorcontrib>Bruneau, Patrick</creatorcontrib><creatorcontrib>Blondel, Bruno</creatorcontrib><creatorcontrib>Crainic, Radu</creatorcontrib><creatorcontrib>Horodniceanu, Florian</creatorcontrib><creatorcontrib>Girard, Marc</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Der Werf, Sylvie</au><au>Wychowski, Czeslaw</au><au>Bruneau, Patrick</au><au>Blondel, Bruno</au><au>Crainic, Radu</au><au>Horodniceanu, Florian</au><au>Girard, Marc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of a Poliovirus Type 1 Neutralization Epitope in Viral Capsid Polypeptide VP1</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1983-08-01</date><risdate>1983</risdate><volume>80</volume><issue>16</issue><spage>5080</spage><epage>5084</epage><pages>5080-5084</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the β -lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-β -lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6308670</pmid><doi>10.1073/pnas.80.16.5080</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1983-08, Vol.80 (16), p.5080-5084 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Animals Antibodies Antibodies, Monoclonal Antigen-Antibody Complex Base Sequence Capsid DNA DNA - analysis DNA Restriction Enzymes Epitopes Escherichia coli - genetics Mice Monoclonal antibodies Nucleotides Plasmids Poliovirus Poliovirus - genetics poliovirus 1 Viral Proteins - genetics Viral Proteins - immunology Viral Structural Proteins Viruses |
| title | Localization of a Poliovirus Type 1 Neutralization Epitope in Viral Capsid Polypeptide VP1 |
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