Decreased de novo Synthesis of Glomerular Proteoglycans in Diabetes: Biochemical and Autoradiographic Evidence
The experimental model of streptozotocin-induced diabetes in rats was utilized to determine the biosynthetic and biochemical alterations in the proteoglycans of the glomerular extracellular matrices (glomerular basement membrane and mesangial matrix) in diabetic nephropathy. Isolated kidneys from di...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1983-04, Vol.80 (8), p.2272-2275 |
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| creator | Kanwar, Yashpal S. Rosenzweig, Lionel J. Linker, Alfred Jakubowski, Michael L. |
| description | The experimental model of streptozotocin-induced diabetes in rats was utilized to determine the biosynthetic and biochemical alterations in the proteoglycans of the glomerular extracellular matrices (glomerular basement membrane and mesangial matrix) in diabetic nephropathy. Isolated kidneys from diabetic and control groups of animals were radiolabeled in an organ perfusion apparatus with [35S]sulfate of high specific activity (>1,200 Ci/mmol; 1 Ci =3.7× 1010Bq) and processed for electron microscopic autoradiography, and the proteoglycans of the glomerular extracellular matrices were characterized. The results indicate that [35S]sulfate incorporation into glomerular extracellular matrices of diabetic animals was 30-40% less than that of the control group; however, no differences in the biochemical properties of the de novo synthesized proteoglycans from either group were observed. The relevance of the decreased de novo synthesis of sulfated proteoglycans of glomerular extracellular matrices is discussed in terms of increased glomerular permeability to plasma proteins and reduction in the glomerular filtration rate. |
| doi_str_mv | 10.1073/pnas.80.8.2272 |
| format | Article |
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Isolated kidneys from diabetic and control groups of animals were radiolabeled in an organ perfusion apparatus with [35S]sulfate of high specific activity (>1,200 Ci/mmol; 1 Ci =3.7× 1010Bq) and processed for electron microscopic autoradiography, and the proteoglycans of the glomerular extracellular matrices were characterized. The results indicate that [35S]sulfate incorporation into glomerular extracellular matrices of diabetic animals was 30-40% less than that of the control group; however, no differences in the biochemical properties of the de novo synthesized proteoglycans from either group were observed. The relevance of the decreased de novo synthesis of sulfated proteoglycans of glomerular extracellular matrices is discussed in terms of increased glomerular permeability to plasma proteins and reduction in the glomerular filtration rate.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.80.8.2272</identifier><identifier>PMID: 6572976</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Autoradiography ; Basement Membrane - metabolism ; Biochemistry ; Control groups ; Diabetes ; Diabetes complications ; Diabetes Mellitus, Experimental - metabolism ; Diabetic nephropathies ; Kidney Glomerulus - metabolism ; Kidneys ; Male ; Perfusion ; Proteoglycans ; Proteoglycans - biosynthesis ; Rats ; Sulfates ; Sulfates - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1983-04, Vol.80 (8), p.2272-2275</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4372-3a04324c6e945b18a7e093538c32e6c49ac91ae919399a36bf4907f41b0662b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/80/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/13493$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/13493$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27902,27903,53768,53770,57994,58227</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6572976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanwar, Yashpal S.</creatorcontrib><creatorcontrib>Rosenzweig, Lionel J.</creatorcontrib><creatorcontrib>Linker, Alfred</creatorcontrib><creatorcontrib>Jakubowski, Michael L.</creatorcontrib><title>Decreased de novo Synthesis of Glomerular Proteoglycans in Diabetes: Biochemical and Autoradiographic Evidence</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The experimental model of streptozotocin-induced diabetes in rats was utilized to determine the biosynthetic and biochemical alterations in the proteoglycans of the glomerular extracellular matrices (glomerular basement membrane and mesangial matrix) in diabetic nephropathy. Isolated kidneys from diabetic and control groups of animals were radiolabeled in an organ perfusion apparatus with [35S]sulfate of high specific activity (>1,200 Ci/mmol; 1 Ci =3.7× 1010Bq) and processed for electron microscopic autoradiography, and the proteoglycans of the glomerular extracellular matrices were characterized. The results indicate that [35S]sulfate incorporation into glomerular extracellular matrices of diabetic animals was 30-40% less than that of the control group; however, no differences in the biochemical properties of the de novo synthesized proteoglycans from either group were observed. The relevance of the decreased de novo synthesis of sulfated proteoglycans of glomerular extracellular matrices is discussed in terms of increased glomerular permeability to plasma proteins and reduction in the glomerular filtration rate.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Basement Membrane - metabolism</subject><subject>Biochemistry</subject><subject>Control groups</subject><subject>Diabetes</subject><subject>Diabetes complications</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetic nephropathies</subject><subject>Kidney Glomerulus - metabolism</subject><subject>Kidneys</subject><subject>Male</subject><subject>Perfusion</subject><subject>Proteoglycans</subject><subject>Proteoglycans - biosynthesis</subject><subject>Rats</subject><subject>Sulfates</subject><subject>Sulfates - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc2P0zAQxS0EWsrClQMSkk_cEvzVJEbisOwuC9JKILF3a-JMWq9cu9hJRf97ErWUInGaw3u_mTd6hLzmrOSslu-3AXLZsLIphajFE7LgTPOiUpo9JQvGRF00Sqjn5EXOj4wxvWzYBbmolrXQdbUg4QZtQsjY0Q5piLtIf-zDsMbsMo09vfNxg2n0kOj3FAeMK7-3EDJ1gd44aHHA_IF-ctGuceMseAqho1fjEBN0Lq4SbNfO0tud6zBYfEme9eAzvjrOS_Lw-fbh-ktx_-3u6_XVfWGVrEUhgSkplK1Qq2XLG6iRabmUjZUCK6s0WM0BNddSa5BV20__1r3iLasq0cpL8vGwdju2G-wshiGBN9vkNpD2JoIz_yrBrc0q7ozUsmF84t8d-RR_jpgHs3HZovcQMI7ZNHM8oeVkLA9Gm2LOCfvTDc7M3I-Z-5n8pjFzPxPw9jzZyX4s5OzyzP1RT7zpR-8H_DWcLfqvcdLfHPTHPHXxN5ZUU-zfgzeuZQ</recordid><startdate>19830401</startdate><enddate>19830401</enddate><creator>Kanwar, Yashpal S.</creator><creator>Rosenzweig, Lionel J.</creator><creator>Linker, Alfred</creator><creator>Jakubowski, Michael L.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19830401</creationdate><title>Decreased de novo Synthesis of Glomerular Proteoglycans in Diabetes: Biochemical and Autoradiographic Evidence</title><author>Kanwar, Yashpal S. ; Rosenzweig, Lionel J. ; Linker, Alfred ; Jakubowski, Michael L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4372-3a04324c6e945b18a7e093538c32e6c49ac91ae919399a36bf4907f41b0662b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Basement Membrane - metabolism</topic><topic>Biochemistry</topic><topic>Control groups</topic><topic>Diabetes</topic><topic>Diabetes complications</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetic nephropathies</topic><topic>Kidney Glomerulus - metabolism</topic><topic>Kidneys</topic><topic>Male</topic><topic>Perfusion</topic><topic>Proteoglycans</topic><topic>Proteoglycans - biosynthesis</topic><topic>Rats</topic><topic>Sulfates</topic><topic>Sulfates - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanwar, Yashpal S.</creatorcontrib><creatorcontrib>Rosenzweig, Lionel J.</creatorcontrib><creatorcontrib>Linker, Alfred</creatorcontrib><creatorcontrib>Jakubowski, Michael L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanwar, Yashpal S.</au><au>Rosenzweig, Lionel J.</au><au>Linker, Alfred</au><au>Jakubowski, Michael L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased de novo Synthesis of Glomerular Proteoglycans in Diabetes: Biochemical and Autoradiographic Evidence</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1983-04-01</date><risdate>1983</risdate><volume>80</volume><issue>8</issue><spage>2272</spage><epage>2275</epage><pages>2272-2275</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The experimental model of streptozotocin-induced diabetes in rats was utilized to determine the biosynthetic and biochemical alterations in the proteoglycans of the glomerular extracellular matrices (glomerular basement membrane and mesangial matrix) in diabetic nephropathy. Isolated kidneys from diabetic and control groups of animals were radiolabeled in an organ perfusion apparatus with [35S]sulfate of high specific activity (>1,200 Ci/mmol; 1 Ci =3.7× 1010Bq) and processed for electron microscopic autoradiography, and the proteoglycans of the glomerular extracellular matrices were characterized. The results indicate that [35S]sulfate incorporation into glomerular extracellular matrices of diabetic animals was 30-40% less than that of the control group; however, no differences in the biochemical properties of the de novo synthesized proteoglycans from either group were observed. The relevance of the decreased de novo synthesis of sulfated proteoglycans of glomerular extracellular matrices is discussed in terms of increased glomerular permeability to plasma proteins and reduction in the glomerular filtration rate.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6572976</pmid><doi>10.1073/pnas.80.8.2272</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1983-04, Vol.80 (8), p.2272-2275 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Autoradiography Basement Membrane - metabolism Biochemistry Control groups Diabetes Diabetes complications Diabetes Mellitus, Experimental - metabolism Diabetic nephropathies Kidney Glomerulus - metabolism Kidneys Male Perfusion Proteoglycans Proteoglycans - biosynthesis Rats Sulfates Sulfates - metabolism |
| title | Decreased de novo Synthesis of Glomerular Proteoglycans in Diabetes: Biochemical and Autoradiographic Evidence |
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