15N NMR Relaxation Studies of Backbone Dynamics in Free and Steroid-Bound Δ5-3-Ketosteroid Isomerase from Pseudomonas testosteroni

The backbone dynamics of Δ5-3-ketosteroid isomerase (KSI) from Pseudomonas testosteroni has been studied in free enzyme and its complex with a steroid ligand, 19-nortestosterone hemisuccinate (19-NTHS), by 15N relaxation measurements. The relaxation data were analyzed using the model-free formalism...

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Veröffentlicht in:	Biochemistry (Easton) 2001-04, Vol.40 (13), p.3967-3973
Hauptverfasser:	Yun, Sunggoo, Jang, Do Soo, Kim, Do-Hyung, Choi, Kwan Yong, Lee, Hee Cheon
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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