Solid phase synthesis of 5′-diphosphorylated oligoribonucleotides and their conversion to capped m7 Gppp-oligoribonucleotides for uase as primers for influenza A virus RNA polymerase in vitro
We have synthesized four different 5′-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotectlon and partial purification the 5′-diphosphorylated oligoribonucleotides could be converted to capped (m7Gppp)-oligoribonucleotides us...
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| Veröffentlicht in: | Nucleic acids research 1995-07, Vol.23 (14), p.2641-2647 |
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| container_issue | 14 |
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| container_title | Nucleic acids research |
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| creator | Brownlee, G.G. Fodor, E. Pritlove, D.C. Gould, K.G. Dalluge, J.J. |
| description | We have synthesized four different 5′-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotectlon and partial purification the 5′-diphosphorylated oligoribonucleotides could be converted to capped (m7Gppp)-oligoribonucleotides using guanylyl transferase. Radlolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. The solid phase method described here should also allow the addition of 5′-diphosphates to synthetic ollgodeoxyribonucleotides and be capable of automation. |
| doi_str_mv | 10.1093/nar/23.14.2641 |
| format | Article |
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After deprotectlon and partial purification the 5′-diphosphorylated oligoribonucleotides could be converted to capped (m7Gppp)-oligoribonucleotides using guanylyl transferase. Radlolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. 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| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1995-07, Vol.23 (14), p.2641-2647 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_istex_primary_ark_67375_HXZ_XD0XVMMP_F |
| source | Oxford University Press Archive; PubMed Central |
| title | Solid phase synthesis of 5′-diphosphorylated oligoribonucleotides and their conversion to capped m7 Gppp-oligoribonucleotides for uase as primers for influenza A virus RNA polymerase in vitro |
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