Flagellasialin: a novel sulfated α2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella

A novel α2,9-linked polysialic acid (polySia)-containing glycoprotein of sea urchin sperm flagella was identified and named "flagellasialin." Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on sodium dodecyl sulfate-polyacrylamide gel electrophoresis...

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Veröffentlicht in:Glycobiology (Oxford) 2006-12, Vol.16 (12), p.1229-1241
Hauptverfasser: Miyata, Shinji, Sato, Chihiro, Kumita, Hironobu, Toriyama, Masaru, Vacquier, Victor D, Kitajima, Ken
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container_end_page 1241
container_issue 12
container_start_page 1229
container_title Glycobiology (Oxford)
container_volume 16
creator Miyata, Shinji
Sato, Chihiro
Kumita, Hironobu
Toriyama, Masaru
Vacquier, Victor D
Kitajima, Ken
description A novel α2,9-linked polysialic acid (polySia)-containing glycoprotein of sea urchin sperm flagella was identified and named "flagellasialin." Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 40-80 kDa. Flagellasialin is a 96-amino acid, threonine-rich, heavily O-glycosylated (80-90% by weight) glycoprotein with a single transmembrane segment at its C-terminus and no apparent cytosolic domain. Of 12 extracellular Thr residues, eight are O-glycosylated and three are nonglycosylated. Flagellasialin is highly expressed in the testis but cannot be detected in the ovary. The amino acid sequences of flagellasialin from three sea urchin species (H. pulcherrimus, Strongylocentrotus purpuratus, and Strongylocentrotus franciscanus) are identical, but some species differences exist in the three core glycan structures to which the sulfated α2,9-linked polyNeu5Ac chain is linked. Finally, the treatment of sperm with a specific antibody against the α2,9-linked polyNeu5Ac structure results in the elevation of intracellular Ca²⁺ and inhibition of sperm motility and fertilization, implicating flagellasialin as a regulator of these critical processes.
doi_str_mv 10.1093/glycob/cwl036
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source Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects fertilization
flagellasialin
polysialic acid
sea urchin
sperm motility
title Flagellasialin: a novel sulfated α2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella
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