Bleomycin mediated degradation of DNA-RNA hybrids does not involve C–1′ chemistry
Incubation of Fe(ll) bleomycin and O2 with a number of ′A′-like DNA-RNA hybrid homopolymers at 4 atm O2 results in formation of base propenal and base In a ratio of approximately 1.0:1.0. This ratio differs dramatically from the corresponding ratio of approximately 10:1.0 observed when activated BLM...
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| Veröffentlicht in: | Nucleic acids research 1992-08, Vol.20 (16), p.4179-4185 |
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| creator | Absalon, M.J. Krishnamoorthy, C.R. McGall, G. Kozarich, J.W. Stubbe, J. |
| description | Incubation of Fe(ll) bleomycin and O2 with a number of ′A′-like DNA-RNA hybrid homopolymers at 4 atm O2 results in formation of base propenal and base In a ratio of approximately 1.0:1.0. This ratio differs dramatically from the corresponding ratio of approximately 10:1.0 observed when activated BLM degrades ′B′-like DNA homopolymers. Experiments were undertaken to detemine if the shift to enhanced base production observed in the A-like hybrids is the result of C-1′ chemistry in addition to the C-4′ chemistry normally observed with B-like DNA under identical conditions. Increased accessibility of the 1′-hydrogen might be anticipated due to widening of the minor groove in the A-like conformers. Experiments using poly([1′-3H]dA) poly(rU) and poly([U-14C]dA) poly(rU indicated that neither 3H2O nor deoxyribonolactone accompanied adenlne release. In addition, studies using poly([4′-2H]dA) poly(rU) and poly([1′-2H]dA) poly(rU) unambiguously establish that the altered base to base propenal ratio is not the result of C–1′chemistry, but a direct consequence of C–4′ chemistry. |
| doi_str_mv | 10.1093/nar/20.16.4179 |
| format | Article |
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This ratio differs dramatically from the corresponding ratio of approximately 10:1.0 observed when activated BLM degrades ′B′-like DNA homopolymers. Experiments were undertaken to detemine if the shift to enhanced base production observed in the A-like hybrids is the result of C-1′ chemistry in addition to the C-4′ chemistry normally observed with B-like DNA under identical conditions. Increased accessibility of the 1′-hydrogen might be anticipated due to widening of the minor groove in the A-like conformers. Experiments using poly([1′-3H]dA) poly(rU) and poly([U-14C]dA) poly(rU indicated that neither 3H2O nor deoxyribonolactone accompanied adenlne release. In addition, studies using poly([4′-2H]dA) poly(rU) and poly([1′-2H]dA) poly(rU) unambiguously establish that the altered base to base propenal ratio is not the result of C–1′chemistry, but a direct consequence of C–4′ chemistry.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/20.16.4179</identifier><identifier>PMID: 1380696</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Bleomycin - chemistry ; Fundamental and applied biological sciences. Psychology ; Molecular and cellular biology ; Molecular genetics ; Mutagenesis. 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This ratio differs dramatically from the corresponding ratio of approximately 10:1.0 observed when activated BLM degrades ′B′-like DNA homopolymers. Experiments were undertaken to detemine if the shift to enhanced base production observed in the A-like hybrids is the result of C-1′ chemistry in addition to the C-4′ chemistry normally observed with B-like DNA under identical conditions. Increased accessibility of the 1′-hydrogen might be anticipated due to widening of the minor groove in the A-like conformers. Experiments using poly([1′-3H]dA) poly(rU) and poly([U-14C]dA) poly(rU indicated that neither 3H2O nor deoxyribonolactone accompanied adenlne release. In addition, studies using poly([4′-2H]dA) poly(rU) and poly([1′-2H]dA) poly(rU) unambiguously establish that the altered base to base propenal ratio is not the result of C–1′chemistry, but a direct consequence of C–4′ chemistry.</description><subject>Biological and medical sciences</subject><subject>Bleomycin - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutagenesis. Repair</subject><subject>Oxygen - chemistry</subject><subject>Polydeoxyribonucleotides - chemistry</subject><subject>Polyribonucleotides - chemistry</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEUhS0EKiGwZYc0C8RuUl9f_4wXLNLwE6QSJKASYmN5ZpzGMBkXexKRXd-BN-GR-iS4ShRg1YXlK53vXB_rEPIU6ASoxtPexlOWZznhoPQ9MgKUrORasvtkRJGKEiivHpJHKX2jFDgIfkJOACsqtRyRi7POhfWu8X2xdq23g2uL1l1G29rBh74Iy-LVYlp-XEyL1a6Ovk1FG1wq-jAUvt-GbuuK2c31L7i5_l00K7f2aYi7x-TB0nbJPTncY3Lx5vXn2bw8__D23Wx6XjYCcChZTsZEjdQy2WpbOa2dlMKhRdBWW3TWMi2E4Fw1StC2XjKOICtkCipV45i83O-92tQ5fuP6IdrOXEW_tnFngvXmf6X3K3MZtgaRA8Psf3Hwx_Bj49Jgcv7GdZ3tXdgkoxC4BIA7QdAopFJ3bwSJFEU-YzLZg00MKUW3PKYGam6bNblZw_IszW2z2fDs37_-xfdVZv35Qbepsd0y2r7x6YgJXtFKqIyVeywX5X4eZRu_G6lQCTP_8tW8n80Xnxb5ZY1_AIXZu9M</recordid><startdate>19920825</startdate><enddate>19920825</enddate><creator>Absalon, M.J.</creator><creator>Krishnamoorthy, C.R.</creator><creator>McGall, G.</creator><creator>Kozarich, J.W.</creator><creator>Stubbe, J.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920825</creationdate><title>Bleomycin mediated degradation of DNA-RNA hybrids does not involve C–1′ chemistry</title><author>Absalon, M.J. ; Krishnamoorthy, C.R. ; McGall, G. ; Kozarich, J.W. ; Stubbe, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-204825b30a26d9a8e99e665e3a319a9a3eaa29555447c750dbf243168327187b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>Bleomycin - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutagenesis. Repair</topic><topic>Oxygen - chemistry</topic><topic>Polydeoxyribonucleotides - chemistry</topic><topic>Polyribonucleotides - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Absalon, M.J.</creatorcontrib><creatorcontrib>Krishnamoorthy, C.R.</creatorcontrib><creatorcontrib>McGall, G.</creatorcontrib><creatorcontrib>Kozarich, J.W.</creatorcontrib><creatorcontrib>Stubbe, J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Absalon, M.J.</au><au>Krishnamoorthy, C.R.</au><au>McGall, G.</au><au>Kozarich, J.W.</au><au>Stubbe, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bleomycin mediated degradation of DNA-RNA hybrids does not involve C–1′ chemistry</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1992-08-25</date><risdate>1992</risdate><volume>20</volume><issue>16</issue><spage>4179</spage><epage>4185</epage><pages>4179-4185</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Incubation of Fe(ll) bleomycin and O2 with a number of ′A′-like DNA-RNA hybrid homopolymers at 4 atm O2 results in formation of base propenal and base In a ratio of approximately 1.0:1.0. 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| subjects | Biological and medical sciences Bleomycin - chemistry Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Mutagenesis. Repair Oxygen - chemistry Polydeoxyribonucleotides - chemistry Polyribonucleotides - chemistry |
| title | Bleomycin mediated degradation of DNA-RNA hybrids does not involve C–1′ chemistry |
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