Human non-secretory ribonucleases. II. Structural characterization of theN-glycans of the kidney, liver and spleen enzymes by NMR spectroscopy and electrospray mass spectrometry

The N-glylycans have been removed by peptide-N-glycosidase F (PNGase F) from purified human non-secretory RNases derived from kidney, liver and spleen. The spleen RNase was purified by two procedures, one of which did not include the usual acid treatment step (0.25 M H2SO4, 45 min, 4°C), to determin...

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Veröffentlicht in:	Glycobiology (Oxford) 1993-06, Vol.3 (3), p.249-259
Hauptverfasser:	Lawrence, Cris W., Little, Pamela A., Little, Brian W., Glushka, John, van Halbeek, Herman, Alhadeff, Jack A.
Format:	Artikel
Sprache:	eng
Schlagworte:	N-glycans non-secretory RNases
Online-Zugang:	Volltext
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