Cytotoxicity of Receptor-Mediated 16α[125I]Iodo-estradiol in Cultured MCF-7 Human Breast Cancer Cells
Therapeutic strategies using 125I-labeled steroid hormones are attractive in light of the estrogen dependence of many human breast cancers and the favorable microdosimetry resulting from 125I decay. We determined the uptake, specific estrogen receptor (ER) binding, and cytotoxicity of 16α-[125.I]iod...
Gespeichert in:
| Veröffentlicht in: | JNCI : Journal of the National Cancer Institute 1989-03, Vol.81 (6), p.437-440 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 440 |
|---|---|
| container_issue | 6 |
| container_start_page | 437 |
| container_title | JNCI : Journal of the National Cancer Institute |
| container_volume | 81 |
| creator | McLaughlin, W. H. Milius, R. A. Pillai, K. M. R. Edasery, J. P. Blumenthal, R. D. Bloomer, W. D. |
| description | Therapeutic strategies using 125I-labeled steroid hormones are attractive in light of the estrogen dependence of many human breast cancers and the favorable microdosimetry resulting from 125I decay. We determined the uptake, specific estrogen receptor (ER) binding, and cytotoxicity of 16α-[125.I]iodoestradiol in cultured MCF-7 human breast cancer cells. The cytotoxicity of receptor-mediated 125I appears to be sufficient in MCF-7 cells to warrant in vivo experimentation. Furthermore, cytotoxicity not specific to ERs is minimal within the dose range necessary for ER saturation and specific cell killing. Competitive toxicity studies using nonradioactive 17β-estradiol demonstrate an unequivocal relationship between ER binding and clonogenic viability. [J Natl Cancer Inst 81:437–440, 1989] |
| doi_str_mv | 10.1093/jnci/81.6.437 |
| format | Article |
| fullrecord | <record><control><sourceid>istex</sourceid><recordid>TN_cdi_istex_primary_ark_67375_HXZ_K5VW45J6_C</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ark_67375_HXZ_K5VW45J6_C</sourcerecordid><originalsourceid>FETCH-LOGICAL-i106t-70d28bcb01f6984d00d2fe2d55a65dce5351989d3f8e723e2c598bd80827b7813</originalsourceid><addsrcrecordid>eNotjMtKAzEARYMoWKtL9_mBtHlMHrPUYJ1qiyC-UGTITDKQOp2UJIX2s_wRv8mC3s3hwOECcEnwhOCSTVdD66eKTMSkYPIIjEghMKIE82MwwphKpJQsTsFZSit8WEmLEej0Poccdr71eQ9DBx9d6zY5RLR01pvsLCTi5_uDUD7_nAcbkEs5GutDD_0A9bbP23iIlnqGJKy2azPA6-hMylCboXURatf36RycdKZP7uKfY_A8u3nSFVo83M711QJ5gkVGEluqmrbBpBOlKiw-eOeo5dwIblvHGSelKi3rlJOUOdryUjVWYUVlIxVhY4D-fn3Kbldvol-buK9N_KqFZJLX1dt7fc9fXgt-J2rNfgHTuVsF</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Cytotoxicity of Receptor-Mediated 16α[125I]Iodo-estradiol in Cultured MCF-7 Human Breast Cancer Cells</title><source>Oxford University Press Journals Digital Archive legacy</source><creator>McLaughlin, W. H. ; Milius, R. A. ; Pillai, K. M. R. ; Edasery, J. P. ; Blumenthal, R. D. ; Bloomer, W. D.</creator><creatorcontrib>McLaughlin, W. H. ; Milius, R. A. ; Pillai, K. M. R. ; Edasery, J. P. ; Blumenthal, R. D. ; Bloomer, W. D.</creatorcontrib><description>Therapeutic strategies using 125I-labeled steroid hormones are attractive in light of the estrogen dependence of many human breast cancers and the favorable microdosimetry resulting from 125I decay. We determined the uptake, specific estrogen receptor (ER) binding, and cytotoxicity of 16α-[125.I]iodoestradiol in cultured MCF-7 human breast cancer cells. The cytotoxicity of receptor-mediated 125I appears to be sufficient in MCF-7 cells to warrant in vivo experimentation. Furthermore, cytotoxicity not specific to ERs is minimal within the dose range necessary for ER saturation and specific cell killing. Competitive toxicity studies using nonradioactive 17β-estradiol demonstrate an unequivocal relationship between ER binding and clonogenic viability. [J Natl Cancer Inst 81:437–440, 1989]</description><identifier>ISSN: 0027-8874</identifier><identifier>EISSN: 1460-2105</identifier><identifier>DOI: 10.1093/jnci/81.6.437</identifier><language>eng</language><publisher>Oxford University Press</publisher><ispartof>JNCI : Journal of the National Cancer Institute, 1989-03, Vol.81 (6), p.437-440</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>McLaughlin, W. H.</creatorcontrib><creatorcontrib>Milius, R. A.</creatorcontrib><creatorcontrib>Pillai, K. M. R.</creatorcontrib><creatorcontrib>Edasery, J. P.</creatorcontrib><creatorcontrib>Blumenthal, R. D.</creatorcontrib><creatorcontrib>Bloomer, W. D.</creatorcontrib><title>Cytotoxicity of Receptor-Mediated 16α[125I]Iodo-estradiol in Cultured MCF-7 Human Breast Cancer Cells</title><title>JNCI : Journal of the National Cancer Institute</title><description>Therapeutic strategies using 125I-labeled steroid hormones are attractive in light of the estrogen dependence of many human breast cancers and the favorable microdosimetry resulting from 125I decay. We determined the uptake, specific estrogen receptor (ER) binding, and cytotoxicity of 16α-[125.I]iodoestradiol in cultured MCF-7 human breast cancer cells. The cytotoxicity of receptor-mediated 125I appears to be sufficient in MCF-7 cells to warrant in vivo experimentation. Furthermore, cytotoxicity not specific to ERs is minimal within the dose range necessary for ER saturation and specific cell killing. Competitive toxicity studies using nonradioactive 17β-estradiol demonstrate an unequivocal relationship between ER binding and clonogenic viability. [J Natl Cancer Inst 81:437–440, 1989]</description><issn>0027-8874</issn><issn>1460-2105</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNotjMtKAzEARYMoWKtL9_mBtHlMHrPUYJ1qiyC-UGTITDKQOp2UJIX2s_wRv8mC3s3hwOECcEnwhOCSTVdD66eKTMSkYPIIjEghMKIE82MwwphKpJQsTsFZSit8WEmLEej0Poccdr71eQ9DBx9d6zY5RLR01pvsLCTi5_uDUD7_nAcbkEs5GutDD_0A9bbP23iIlnqGJKy2azPA6-hMylCboXURatf36RycdKZP7uKfY_A8u3nSFVo83M711QJ5gkVGEluqmrbBpBOlKiw-eOeo5dwIblvHGSelKi3rlJOUOdryUjVWYUVlIxVhY4D-fn3Kbldvol-buK9N_KqFZJLX1dt7fc9fXgt-J2rNfgHTuVsF</recordid><startdate>19890315</startdate><enddate>19890315</enddate><creator>McLaughlin, W. H.</creator><creator>Milius, R. A.</creator><creator>Pillai, K. M. R.</creator><creator>Edasery, J. P.</creator><creator>Blumenthal, R. D.</creator><creator>Bloomer, W. D.</creator><general>Oxford University Press</general><scope>BSCLL</scope></search><sort><creationdate>19890315</creationdate><title>Cytotoxicity of Receptor-Mediated 16α[125I]Iodo-estradiol in Cultured MCF-7 Human Breast Cancer Cells</title><author>McLaughlin, W. H. ; Milius, R. A. ; Pillai, K. M. R. ; Edasery, J. P. ; Blumenthal, R. D. ; Bloomer, W. D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i106t-70d28bcb01f6984d00d2fe2d55a65dce5351989d3f8e723e2c598bd80827b7813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McLaughlin, W. H.</creatorcontrib><creatorcontrib>Milius, R. A.</creatorcontrib><creatorcontrib>Pillai, K. M. R.</creatorcontrib><creatorcontrib>Edasery, J. P.</creatorcontrib><creatorcontrib>Blumenthal, R. D.</creatorcontrib><creatorcontrib>Bloomer, W. D.</creatorcontrib><collection>Istex</collection><jtitle>JNCI : Journal of the National Cancer Institute</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McLaughlin, W. H.</au><au>Milius, R. A.</au><au>Pillai, K. M. R.</au><au>Edasery, J. P.</au><au>Blumenthal, R. D.</au><au>Bloomer, W. D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity of Receptor-Mediated 16α[125I]Iodo-estradiol in Cultured MCF-7 Human Breast Cancer Cells</atitle><jtitle>JNCI : Journal of the National Cancer Institute</jtitle><date>1989-03-15</date><risdate>1989</risdate><volume>81</volume><issue>6</issue><spage>437</spage><epage>440</epage><pages>437-440</pages><issn>0027-8874</issn><eissn>1460-2105</eissn><abstract>Therapeutic strategies using 125I-labeled steroid hormones are attractive in light of the estrogen dependence of many human breast cancers and the favorable microdosimetry resulting from 125I decay. We determined the uptake, specific estrogen receptor (ER) binding, and cytotoxicity of 16α-[125.I]iodoestradiol in cultured MCF-7 human breast cancer cells. The cytotoxicity of receptor-mediated 125I appears to be sufficient in MCF-7 cells to warrant in vivo experimentation. Furthermore, cytotoxicity not specific to ERs is minimal within the dose range necessary for ER saturation and specific cell killing. Competitive toxicity studies using nonradioactive 17β-estradiol demonstrate an unequivocal relationship between ER binding and clonogenic viability. [J Natl Cancer Inst 81:437–440, 1989]</abstract><pub>Oxford University Press</pub><doi>10.1093/jnci/81.6.437</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8874 |
| ispartof | JNCI : Journal of the National Cancer Institute, 1989-03, Vol.81 (6), p.437-440 |
| issn | 0027-8874 1460-2105 |
| language | eng |
| recordid | cdi_istex_primary_ark_67375_HXZ_K5VW45J6_C |
| source | Oxford University Press Journals Digital Archive legacy |
| title | Cytotoxicity of Receptor-Mediated 16α[125I]Iodo-estradiol in Cultured MCF-7 Human Breast Cancer Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T06%3A49%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-istex&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cytotoxicity%20of%20Receptor-Mediated%2016%CE%B1%5B125I%5DIodo-estradiol%20in%20Cultured%20MCF-7%20Human%20Breast%20Cancer%20Cells&rft.jtitle=JNCI%20:%20Journal%20of%20the%20National%20Cancer%20Institute&rft.au=McLaughlin,%20W.%20H.&rft.date=1989-03-15&rft.volume=81&rft.issue=6&rft.spage=437&rft.epage=440&rft.pages=437-440&rft.issn=0027-8874&rft.eissn=1460-2105&rft_id=info:doi/10.1093/jnci/81.6.437&rft_dat=%3Cistex%3Eark_67375_HXZ_K5VW45J6_C%3C/istex%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |