Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study
A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide Fe CN 6 − 3 − 4 a redox couple, at room temperature (∼25 °C), under the effect of mechanical vibra...
Gespeichert in:
| Veröffentlicht in: | Journal of the Electrochemical Society 2021-01, Vol.168 (1), p.17505 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 17505 |
| container_title | Journal of the Electrochemical Society |
| container_volume | 168 |
| creator | Maurya, Ranjan Kumar Alam, M. A. Ahamad, Nadeem Kishore, Kaushal Prajesh, Rahul Choudhary, Megha Bhalla, Vijayender Agarwal, Ajay |
| description | A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide Fe CN 6 − 3 − 4 a redox couple, at room temperature (∼25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ∼332% (from 3.125 nA M − 1 to 13.5 nA M − 1 ) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ∼15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper. |
| doi_str_mv | 10.1149/1945-7111/abd929 |
| format | Article |
| fullrecord | <record><control><sourceid>iop_cross</sourceid><recordid>TN_cdi_iop_journals_10_1149_1945_7111_abd929</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>jesabd929</sourcerecordid><originalsourceid>FETCH-LOGICAL-c313t-2795f67ae4003256c32a58b2be4ffc5c4b87001616cf1c734683891791ceb6f13</originalsourceid><addsrcrecordid>eNp9kM1LAzEQxYMoWKt3jzlWcDWzyWZ3vdW1fkChharXkE0Tm9Julk0q9OS_bsqKJ_EwDPP4vWHmIXQJ5AaAlbdQsizJAeBW1ssyLY_Q4Fc6RgNCgCaMZ3CKzrxfxxEKlg_Q10I33gb7acMeT5qVbJTe6iZgZ_Bko1XonFrprVVyg--t85F2HTax5s72WCU7jUfzWXWFx227iWiwrvF3-N3icRPsh27wgw5xV5Sx9FhGi9d4EXbL_Tk6MXLj9cVPH6K3x8lr9ZxMZ08v1XiaKAo0JGleZobnUjNCaJpxRVOZFXVaa2aMyhSrizz-xIErAyqnjBe0KCEvQemaG6BDRPq9qnPed9qItrNb2e0FEHEIUBzSEoe0RB9gtFz3FutasXa7rokH_oeP_sDXOlp4IUAQyDOSiXZp6DfdLH8i</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study</title><source>IOP Publishing Journals</source><creator>Maurya, Ranjan Kumar ; Alam, M. A. ; Ahamad, Nadeem ; Kishore, Kaushal ; Prajesh, Rahul ; Choudhary, Megha ; Bhalla, Vijayender ; Agarwal, Ajay</creator><creatorcontrib>Maurya, Ranjan Kumar ; Alam, M. A. ; Ahamad, Nadeem ; Kishore, Kaushal ; Prajesh, Rahul ; Choudhary, Megha ; Bhalla, Vijayender ; Agarwal, Ajay</creatorcontrib><description>A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide Fe CN 6 − 3 − 4 a redox couple, at room temperature (∼25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ∼332% (from 3.125 nA M − 1 to 13.5 nA M − 1 ) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ∼15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper.</description><identifier>ISSN: 0013-4651</identifier><identifier>EISSN: 1945-7111</identifier><identifier>DOI: 10.1149/1945-7111/abd929</identifier><identifier>CODEN: JESOAN</identifier><language>eng</language><publisher>IOP Publishing</publisher><subject>Biosensor ; Electrochemical sensor ; mechanical vibration ; Microfabrication ; POC ; Vi antigen</subject><ispartof>Journal of the Electrochemical Society, 2021-01, Vol.168 (1), p.17505</ispartof><rights>2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-2795f67ae4003256c32a58b2be4ffc5c4b87001616cf1c734683891791ceb6f13</citedby><cites>FETCH-LOGICAL-c313t-2795f67ae4003256c32a58b2be4ffc5c4b87001616cf1c734683891791ceb6f13</cites><orcidid>0000-0001-8882-5362</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1149/1945-7111/abd929/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,776,780,27901,27902,53821</link.rule.ids></links><search><creatorcontrib>Maurya, Ranjan Kumar</creatorcontrib><creatorcontrib>Alam, M. A.</creatorcontrib><creatorcontrib>Ahamad, Nadeem</creatorcontrib><creatorcontrib>Kishore, Kaushal</creatorcontrib><creatorcontrib>Prajesh, Rahul</creatorcontrib><creatorcontrib>Choudhary, Megha</creatorcontrib><creatorcontrib>Bhalla, Vijayender</creatorcontrib><creatorcontrib>Agarwal, Ajay</creatorcontrib><title>Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study</title><title>Journal of the Electrochemical Society</title><addtitle>JES</addtitle><addtitle>J. Electrochem. Soc</addtitle><description>A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide Fe CN 6 − 3 − 4 a redox couple, at room temperature (∼25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ∼332% (from 3.125 nA M − 1 to 13.5 nA M − 1 ) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ∼15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper.</description><subject>Biosensor</subject><subject>Electrochemical sensor</subject><subject>mechanical vibration</subject><subject>Microfabrication</subject><subject>POC</subject><subject>Vi antigen</subject><issn>0013-4651</issn><issn>1945-7111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kM1LAzEQxYMoWKt3jzlWcDWzyWZ3vdW1fkChharXkE0Tm9Julk0q9OS_bsqKJ_EwDPP4vWHmIXQJ5AaAlbdQsizJAeBW1ssyLY_Q4Fc6RgNCgCaMZ3CKzrxfxxEKlg_Q10I33gb7acMeT5qVbJTe6iZgZ_Bko1XonFrprVVyg--t85F2HTax5s72WCU7jUfzWXWFx227iWiwrvF3-N3icRPsh27wgw5xV5Sx9FhGi9d4EXbL_Tk6MXLj9cVPH6K3x8lr9ZxMZ08v1XiaKAo0JGleZobnUjNCaJpxRVOZFXVaa2aMyhSrizz-xIErAyqnjBe0KCEvQemaG6BDRPq9qnPed9qItrNb2e0FEHEIUBzSEoe0RB9gtFz3FutasXa7rokH_oeP_sDXOlp4IUAQyDOSiXZp6DfdLH8i</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Maurya, Ranjan Kumar</creator><creator>Alam, M. A.</creator><creator>Ahamad, Nadeem</creator><creator>Kishore, Kaushal</creator><creator>Prajesh, Rahul</creator><creator>Choudhary, Megha</creator><creator>Bhalla, Vijayender</creator><creator>Agarwal, Ajay</creator><general>IOP Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-8882-5362</orcidid></search><sort><creationdate>20210101</creationdate><title>Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study</title><author>Maurya, Ranjan Kumar ; Alam, M. A. ; Ahamad, Nadeem ; Kishore, Kaushal ; Prajesh, Rahul ; Choudhary, Megha ; Bhalla, Vijayender ; Agarwal, Ajay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-2795f67ae4003256c32a58b2be4ffc5c4b87001616cf1c734683891791ceb6f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biosensor</topic><topic>Electrochemical sensor</topic><topic>mechanical vibration</topic><topic>Microfabrication</topic><topic>POC</topic><topic>Vi antigen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maurya, Ranjan Kumar</creatorcontrib><creatorcontrib>Alam, M. A.</creatorcontrib><creatorcontrib>Ahamad, Nadeem</creatorcontrib><creatorcontrib>Kishore, Kaushal</creatorcontrib><creatorcontrib>Prajesh, Rahul</creatorcontrib><creatorcontrib>Choudhary, Megha</creatorcontrib><creatorcontrib>Bhalla, Vijayender</creatorcontrib><creatorcontrib>Agarwal, Ajay</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of the Electrochemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maurya, Ranjan Kumar</au><au>Alam, M. A.</au><au>Ahamad, Nadeem</au><au>Kishore, Kaushal</au><au>Prajesh, Rahul</au><au>Choudhary, Megha</au><au>Bhalla, Vijayender</au><au>Agarwal, Ajay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study</atitle><jtitle>Journal of the Electrochemical Society</jtitle><stitle>JES</stitle><addtitle>J. Electrochem. Soc</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>168</volume><issue>1</issue><spage>17505</spage><pages>17505-</pages><issn>0013-4651</issn><eissn>1945-7111</eissn><coden>JESOAN</coden><abstract>A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide Fe CN 6 − 3 − 4 a redox couple, at room temperature (∼25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ∼332% (from 3.125 nA M − 1 to 13.5 nA M − 1 ) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ∼15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper.</abstract><pub>IOP Publishing</pub><doi>10.1149/1945-7111/abd929</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-8882-5362</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-4651 |
| ispartof | Journal of the Electrochemical Society, 2021-01, Vol.168 (1), p.17505 |
| issn | 0013-4651 1945-7111 |
| language | eng |
| recordid | cdi_iop_journals_10_1149_1945_7111_abd929 |
| source | IOP Publishing Journals |
| subjects | Biosensor Electrochemical sensor mechanical vibration Microfabrication POC Vi antigen |
| title | Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T18%3A52%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-iop_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensitivity%20Enhancement%20of%20Electrochemical%20Biosensor%20for%20Point%20of%20Care%20(POC)%20Applications:%20Vi%20Antigen%20Detection%20as%20a%20Case%20Study&rft.jtitle=Journal%20of%20the%20Electrochemical%20Society&rft.au=Maurya,%20Ranjan%20Kumar&rft.date=2021-01-01&rft.volume=168&rft.issue=1&rft.spage=17505&rft.pages=17505-&rft.issn=0013-4651&rft.eissn=1945-7111&rft.coden=JESOAN&rft_id=info:doi/10.1149/1945-7111/abd929&rft_dat=%3Ciop_cross%3Ejesabd929%3C/iop_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |