Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly
Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we...
Gespeichert in:
| Veröffentlicht in: | Biofabrication 2018-06, Vol.10 (3), p.034104-034104 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 034104 |
|---|---|
| container_issue | 3 |
| container_start_page | 034104 |
| container_title | Biofabrication |
| container_volume | 10 |
| creator | Parfenov, Vladislav A Koudan, Elizaveta V Bulanova, Elena A Karalkin, Pavel A DAS Pereira, Frederico Norkin, Nikita E Knyazeva, Alisa D Gryadunova, Anna A Petrov, Oleg F Vasiliev, Mikhail M Myasnikov, Maxim I Chernikov, Valery P Kasyanov, Vladimir A Marchenkov, Artem Yu Brakke, Kenn Khesuani, Yusef D Demirci, Utkan Mironov, Vladimir A |
| description | Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology. Label-free magnetic levitation was performed using a prototype device equipped with permanent magnets in presence of gadolinium (Gd3+) in culture media, which enables magnetic levitation. Mathematical modeling and computer simulations were used for prediction of magnetic field and kinetics of tissue spheroids assembly into 3D tissue constructs. First, we used polystyrene beads to simulate the assembly of tissue spheroids and to determine the optimal settings for magnetic levitation in presence of Gd3+. Second, we proved the ability of chondrospheres to assemble rapidly into 3D tissue construct in the permanent magnetic field in the presence of Gd3+. Thus, scaffold- and label-free magnetic levitation of tissue spheroids is a promising approach for rapid 3D biofabrication and attractive alternative to label-based magnetic force-driven tissue engineering. |
| doi_str_mv | 10.1088/1758-5090/aac900 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_iop_j</sourceid><recordid>TN_cdi_iop_journals_10_1088_1758_5090_aac900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2047921097</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-64a5da63ac4562324cc45c5954af8b461d6c4b6ecf9ddab45428908d199fb3493</originalsourceid><addsrcrecordid>eNp9kDtPwzAUhS0EolDYmVAmxNCAndhpPKKKl1SJAZit61fryolLnCC1v56UAGJATD6--s4ZPoTOCL4iuCyvyZSVKcMcXwMojvEeOvo57f_KI3Qc4wrjgrGCHKJRxktaTnl-hJbPCqwNXqe2MWaSeJDGf-YEap3UYbv1ZvhLFyzIxiloXaiT1qhlHXxYbJIuunqRVLCoTetU4s27az8h8AnEaCrpNyfowIKP5vTrHaPXu9uX2UM6f7p_nN3MU0UxbdOCAtNQ5KAoK7I8o6oPinFGwZaSFkQXisrCKMu1BkkZzUqOS004tzKnPB-jy2F33YS3zsRWVC4q4z3UJnRRZJhOeUYwn_YoHlDVhBgbY8W6cRU0G0Gw2PkVO4FiJ1AMfvvK-dd6JyujfwrfQntgMgAurMUqdE3vIP63d_EHLu2ukQucU4KpWGubfwA5e5Jh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2047921097</pqid></control><display><type>article</type><title>Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly</title><source>MEDLINE</source><source>IOP Publishing Journals</source><source>Institute of Physics (IOP) Journals - HEAL-Link</source><creator>Parfenov, Vladislav A ; Koudan, Elizaveta V ; Bulanova, Elena A ; Karalkin, Pavel A ; DAS Pereira, Frederico ; Norkin, Nikita E ; Knyazeva, Alisa D ; Gryadunova, Anna A ; Petrov, Oleg F ; Vasiliev, Mikhail M ; Myasnikov, Maxim I ; Chernikov, Valery P ; Kasyanov, Vladimir A ; Marchenkov, Artem Yu ; Brakke, Kenn ; Khesuani, Yusef D ; Demirci, Utkan ; Mironov, Vladimir A</creator><creatorcontrib>Parfenov, Vladislav A ; Koudan, Elizaveta V ; Bulanova, Elena A ; Karalkin, Pavel A ; DAS Pereira, Frederico ; Norkin, Nikita E ; Knyazeva, Alisa D ; Gryadunova, Anna A ; Petrov, Oleg F ; Vasiliev, Mikhail M ; Myasnikov, Maxim I ; Chernikov, Valery P ; Kasyanov, Vladimir A ; Marchenkov, Artem Yu ; Brakke, Kenn ; Khesuani, Yusef D ; Demirci, Utkan ; Mironov, Vladimir A</creatorcontrib><description>Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology. Label-free magnetic levitation was performed using a prototype device equipped with permanent magnets in presence of gadolinium (Gd3+) in culture media, which enables magnetic levitation. Mathematical modeling and computer simulations were used for prediction of magnetic field and kinetics of tissue spheroids assembly into 3D tissue constructs. First, we used polystyrene beads to simulate the assembly of tissue spheroids and to determine the optimal settings for magnetic levitation in presence of Gd3+. Second, we proved the ability of chondrospheres to assemble rapidly into 3D tissue construct in the permanent magnetic field in the presence of Gd3+. Thus, scaffold- and label-free magnetic levitation of tissue spheroids is a promising approach for rapid 3D biofabrication and attractive alternative to label-based magnetic force-driven tissue engineering.</description><identifier>ISSN: 1758-5090</identifier><identifier>EISSN: 1758-5090</identifier><identifier>DOI: 10.1088/1758-5090/aac900</identifier><identifier>PMID: 29848793</identifier><identifier>CODEN: BIOFCK</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Animals ; biofabrication ; Cell Culture Techniques - instrumentation ; Chondrocytes - cytology ; chondrospheres ; Computer Simulation ; Equipment Design ; gadolinium (Gd ; label-free ; Magnetic Fields ; magnetic levitation (MagLev) ; scaffold-free ; Spheroids, Cellular - cytology ; Tissue Engineering - instrumentation ; tissue spheroids</subject><ispartof>Biofabrication, 2018-06, Vol.10 (3), p.034104-034104</ispartof><rights>2018 IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-64a5da63ac4562324cc45c5954af8b461d6c4b6ecf9ddab45428908d199fb3493</citedby><cites>FETCH-LOGICAL-c404t-64a5da63ac4562324cc45c5954af8b461d6c4b6ecf9ddab45428908d199fb3493</cites><orcidid>0000-0001-8460-3113 ; 0000-0003-1234-0208 ; 0000-0002-0425-1152</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1758-5090/aac900/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,776,780,27901,27902,53821,53868</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29848793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parfenov, Vladislav A</creatorcontrib><creatorcontrib>Koudan, Elizaveta V</creatorcontrib><creatorcontrib>Bulanova, Elena A</creatorcontrib><creatorcontrib>Karalkin, Pavel A</creatorcontrib><creatorcontrib>DAS Pereira, Frederico</creatorcontrib><creatorcontrib>Norkin, Nikita E</creatorcontrib><creatorcontrib>Knyazeva, Alisa D</creatorcontrib><creatorcontrib>Gryadunova, Anna A</creatorcontrib><creatorcontrib>Petrov, Oleg F</creatorcontrib><creatorcontrib>Vasiliev, Mikhail M</creatorcontrib><creatorcontrib>Myasnikov, Maxim I</creatorcontrib><creatorcontrib>Chernikov, Valery P</creatorcontrib><creatorcontrib>Kasyanov, Vladimir A</creatorcontrib><creatorcontrib>Marchenkov, Artem Yu</creatorcontrib><creatorcontrib>Brakke, Kenn</creatorcontrib><creatorcontrib>Khesuani, Yusef D</creatorcontrib><creatorcontrib>Demirci, Utkan</creatorcontrib><creatorcontrib>Mironov, Vladimir A</creatorcontrib><title>Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly</title><title>Biofabrication</title><addtitle>BF</addtitle><addtitle>Biofabrication</addtitle><description>Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology. Label-free magnetic levitation was performed using a prototype device equipped with permanent magnets in presence of gadolinium (Gd3+) in culture media, which enables magnetic levitation. Mathematical modeling and computer simulations were used for prediction of magnetic field and kinetics of tissue spheroids assembly into 3D tissue constructs. First, we used polystyrene beads to simulate the assembly of tissue spheroids and to determine the optimal settings for magnetic levitation in presence of Gd3+. Second, we proved the ability of chondrospheres to assemble rapidly into 3D tissue construct in the permanent magnetic field in the presence of Gd3+. Thus, scaffold- and label-free magnetic levitation of tissue spheroids is a promising approach for rapid 3D biofabrication and attractive alternative to label-based magnetic force-driven tissue engineering.</description><subject>Animals</subject><subject>biofabrication</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Chondrocytes - cytology</subject><subject>chondrospheres</subject><subject>Computer Simulation</subject><subject>Equipment Design</subject><subject>gadolinium (Gd</subject><subject>label-free</subject><subject>Magnetic Fields</subject><subject>magnetic levitation (MagLev)</subject><subject>scaffold-free</subject><subject>Spheroids, Cellular - cytology</subject><subject>Tissue Engineering - instrumentation</subject><subject>tissue spheroids</subject><issn>1758-5090</issn><issn>1758-5090</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAUhS0EolDYmVAmxNCAndhpPKKKl1SJAZit61fryolLnCC1v56UAGJATD6--s4ZPoTOCL4iuCyvyZSVKcMcXwMojvEeOvo57f_KI3Qc4wrjgrGCHKJRxktaTnl-hJbPCqwNXqe2MWaSeJDGf-YEap3UYbv1ZvhLFyzIxiloXaiT1qhlHXxYbJIuunqRVLCoTetU4s27az8h8AnEaCrpNyfowIKP5vTrHaPXu9uX2UM6f7p_nN3MU0UxbdOCAtNQ5KAoK7I8o6oPinFGwZaSFkQXisrCKMu1BkkZzUqOS004tzKnPB-jy2F33YS3zsRWVC4q4z3UJnRRZJhOeUYwn_YoHlDVhBgbY8W6cRU0G0Gw2PkVO4FiJ1AMfvvK-dd6JyujfwrfQntgMgAurMUqdE3vIP63d_EHLu2ukQucU4KpWGubfwA5e5Jh</recordid><startdate>20180618</startdate><enddate>20180618</enddate><creator>Parfenov, Vladislav A</creator><creator>Koudan, Elizaveta V</creator><creator>Bulanova, Elena A</creator><creator>Karalkin, Pavel A</creator><creator>DAS Pereira, Frederico</creator><creator>Norkin, Nikita E</creator><creator>Knyazeva, Alisa D</creator><creator>Gryadunova, Anna A</creator><creator>Petrov, Oleg F</creator><creator>Vasiliev, Mikhail M</creator><creator>Myasnikov, Maxim I</creator><creator>Chernikov, Valery P</creator><creator>Kasyanov, Vladimir A</creator><creator>Marchenkov, Artem Yu</creator><creator>Brakke, Kenn</creator><creator>Khesuani, Yusef D</creator><creator>Demirci, Utkan</creator><creator>Mironov, Vladimir A</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8460-3113</orcidid><orcidid>https://orcid.org/0000-0003-1234-0208</orcidid><orcidid>https://orcid.org/0000-0002-0425-1152</orcidid></search><sort><creationdate>20180618</creationdate><title>Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly</title><author>Parfenov, Vladislav A ; Koudan, Elizaveta V ; Bulanova, Elena A ; Karalkin, Pavel A ; DAS Pereira, Frederico ; Norkin, Nikita E ; Knyazeva, Alisa D ; Gryadunova, Anna A ; Petrov, Oleg F ; Vasiliev, Mikhail M ; Myasnikov, Maxim I ; Chernikov, Valery P ; Kasyanov, Vladimir A ; Marchenkov, Artem Yu ; Brakke, Kenn ; Khesuani, Yusef D ; Demirci, Utkan ; Mironov, Vladimir A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-64a5da63ac4562324cc45c5954af8b461d6c4b6ecf9ddab45428908d199fb3493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>biofabrication</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Chondrocytes - cytology</topic><topic>chondrospheres</topic><topic>Computer Simulation</topic><topic>Equipment Design</topic><topic>gadolinium (Gd</topic><topic>label-free</topic><topic>Magnetic Fields</topic><topic>magnetic levitation (MagLev)</topic><topic>scaffold-free</topic><topic>Spheroids, Cellular - cytology</topic><topic>Tissue Engineering - instrumentation</topic><topic>tissue spheroids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parfenov, Vladislav A</creatorcontrib><creatorcontrib>Koudan, Elizaveta V</creatorcontrib><creatorcontrib>Bulanova, Elena A</creatorcontrib><creatorcontrib>Karalkin, Pavel A</creatorcontrib><creatorcontrib>DAS Pereira, Frederico</creatorcontrib><creatorcontrib>Norkin, Nikita E</creatorcontrib><creatorcontrib>Knyazeva, Alisa D</creatorcontrib><creatorcontrib>Gryadunova, Anna A</creatorcontrib><creatorcontrib>Petrov, Oleg F</creatorcontrib><creatorcontrib>Vasiliev, Mikhail M</creatorcontrib><creatorcontrib>Myasnikov, Maxim I</creatorcontrib><creatorcontrib>Chernikov, Valery P</creatorcontrib><creatorcontrib>Kasyanov, Vladimir A</creatorcontrib><creatorcontrib>Marchenkov, Artem Yu</creatorcontrib><creatorcontrib>Brakke, Kenn</creatorcontrib><creatorcontrib>Khesuani, Yusef D</creatorcontrib><creatorcontrib>Demirci, Utkan</creatorcontrib><creatorcontrib>Mironov, Vladimir A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biofabrication</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parfenov, Vladislav A</au><au>Koudan, Elizaveta V</au><au>Bulanova, Elena A</au><au>Karalkin, Pavel A</au><au>DAS Pereira, Frederico</au><au>Norkin, Nikita E</au><au>Knyazeva, Alisa D</au><au>Gryadunova, Anna A</au><au>Petrov, Oleg F</au><au>Vasiliev, Mikhail M</au><au>Myasnikov, Maxim I</au><au>Chernikov, Valery P</au><au>Kasyanov, Vladimir A</au><au>Marchenkov, Artem Yu</au><au>Brakke, Kenn</au><au>Khesuani, Yusef D</au><au>Demirci, Utkan</au><au>Mironov, Vladimir A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly</atitle><jtitle>Biofabrication</jtitle><stitle>BF</stitle><addtitle>Biofabrication</addtitle><date>2018-06-18</date><risdate>2018</risdate><volume>10</volume><issue>3</issue><spage>034104</spage><epage>034104</epage><pages>034104-034104</pages><issn>1758-5090</issn><eissn>1758-5090</eissn><coden>BIOFCK</coden><abstract>Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology. Label-free magnetic levitation was performed using a prototype device equipped with permanent magnets in presence of gadolinium (Gd3+) in culture media, which enables magnetic levitation. Mathematical modeling and computer simulations were used for prediction of magnetic field and kinetics of tissue spheroids assembly into 3D tissue constructs. First, we used polystyrene beads to simulate the assembly of tissue spheroids and to determine the optimal settings for magnetic levitation in presence of Gd3+. Second, we proved the ability of chondrospheres to assemble rapidly into 3D tissue construct in the permanent magnetic field in the presence of Gd3+. Thus, scaffold- and label-free magnetic levitation of tissue spheroids is a promising approach for rapid 3D biofabrication and attractive alternative to label-based magnetic force-driven tissue engineering.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>29848793</pmid><doi>10.1088/1758-5090/aac900</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-8460-3113</orcidid><orcidid>https://orcid.org/0000-0003-1234-0208</orcidid><orcidid>https://orcid.org/0000-0002-0425-1152</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1758-5090 |
| ispartof | Biofabrication, 2018-06, Vol.10 (3), p.034104-034104 |
| issn | 1758-5090 1758-5090 |
| language | eng |
| recordid | cdi_iop_journals_10_1088_1758_5090_aac900 |
| source | MEDLINE; IOP Publishing Journals; Institute of Physics (IOP) Journals - HEAL-Link |
| subjects | Animals biofabrication Cell Culture Techniques - instrumentation Chondrocytes - cytology chondrospheres Computer Simulation Equipment Design gadolinium (Gd label-free Magnetic Fields magnetic levitation (MagLev) scaffold-free Spheroids, Cellular - cytology Tissue Engineering - instrumentation tissue spheroids |
| title | Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T23%3A33%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_iop_j&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Scaffold-free,%20label-free%20and%20nozzle-free%20biofabrication%20technology%20using%20magnetic%20levitational%20assembly&rft.jtitle=Biofabrication&rft.au=Parfenov,%20Vladislav%20A&rft.date=2018-06-18&rft.volume=10&rft.issue=3&rft.spage=034104&rft.epage=034104&rft.pages=034104-034104&rft.issn=1758-5090&rft.eissn=1758-5090&rft.coden=BIOFCK&rft_id=info:doi/10.1088/1758-5090/aac900&rft_dat=%3Cproquest_iop_j%3E2047921097%3C/proquest_iop_j%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2047921097&rft_id=info:pmid/29848793&rfr_iscdi=true |