Fabrication and application of salicin-polycaprolactone 3D-printed scaffold in the healing of femur bone defects

Polycaprolactone (PCL) is a suitable material for bone repair due to good biocompatibility and mechanical properties. However, low bioactivity and hydrophobicity pose major challenges for its biomedical applications. To overcome these limitations, PCL-based scaffolds loaded with bioactive agents hav...

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Veröffentlicht in:Biomedical materials (Bristol) 2024-05, Vol.19 (3), p.35020
Hauptverfasser: Jalali, Hanieh, Salemian, Milad, Nabiuni, Mohammad, Kouchesfehani, Homa Mohseni, Bardei, Latifeh Karimzadeh, Gregory, Carl
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container_issue 3
container_start_page 35020
container_title Biomedical materials (Bristol)
container_volume 19
creator Jalali, Hanieh
Salemian, Milad
Nabiuni, Mohammad
Kouchesfehani, Homa Mohseni
Bardei, Latifeh Karimzadeh
Gregory, Carl
description Polycaprolactone (PCL) is a suitable material for bone repair due to good biocompatibility and mechanical properties. However, low bioactivity and hydrophobicity pose major challenges for its biomedical applications. To overcome these limitations, PCL-based scaffolds loaded with bioactive agents have been developed. Salicin (Sal) is an anti-inflammatory and analgesic herbal glycoside with osteogenic potential. In the present study, we aimed to produce a Sal-laden PCL (PCL-Sal) scaffold for bone healing applications. Three-dimensional scaffolds were produced and their biocompatibility, and physical-chemical characteristics were determined. The osteogenic potential of the PCL (PCL) and PCL-Sal scaffolds was evaluated using bone marrow mesenchymal stem cells (BMSCs). Scaffolds were implanted into a 5 mm bone defect created in the femur of adult rats, and the new bone fraction was determined using micro-computed tomography scanning at one-month follow-up. PCL-Sal scaffold had a structure, porosity, and fiber diameter suitable for bone construction. It also possessed a higher rate of hydrophilicity and bioactivity compared to the PCL, providing a suitable surface for the proliferation and bone differentiation of BMSCs. Furthermore, PCL-Sal scaffolds showed a higher capacity to scavenge free radicals compared to PCL. The improved bone healing potential of the PCL-Sal scaffold was also confirmed according to implantation results. Our findings revealed that the Sal-laden implant could be considered for bone repair due to desirable characteristics of Sal such as hydrophilicity, surface modification for cell attachment, and antioxidant properties.
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source MEDLINE; Institute of Physics Journals
subjects 3D-printing
Animals
Benzyl Alcohols
bone
Femur
Glucosides
mesenchymal stem cell
Osteogenesis
polycaprolactone
Polyesters - chemistry
Printing, Three-Dimensional
Rats
regeneration
salicylates
Tissue Scaffolds - chemistry
X-Ray Microtomography
title Fabrication and application of salicin-polycaprolactone 3D-printed scaffold in the healing of femur bone defects
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