Molecular Cloning and Sequence Analysis of the cDNA Encoding Beta-Lactoglobulin in Bubalus bubalis

Molecular Cloning and Sequence Analysis of the cDNA Encoding Beta-Lactoglobulin in Bubalus bubalis

The cDNA for bubaline beta-lactoglobulin (plg) has been cloned through RT-PCR approach and sequenced. Sequence data showed a single open reading frame coding for a protein of 180 amino acids with a signal sequence of 18 amino acid residues. Comparison with other ruminant β1g sequences revealed a hig...

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Veröffentlicht in:DNA sequence 1999, Vol.10 (2), p.105-108
Hauptverfasser: Das, Paramananda, Jain, Sudhir, Nayak, Sumitra, Apparao, K. B. C., Totey, S. M., Garg, Lalit C.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Animals
Base Sequence
Beta-lactoglobulin
Buffaloes - genetics
Cattle
cDNA cloning
Cloning, Molecular
DNA, Complementary
Lactoglobulins - genetics
Molecular Sequence Data
RT-PCR
Sequence Analysis, DNA
Sequencing
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Zusammenfassung:The cDNA for bubaline beta-lactoglobulin (plg) has been cloned through RT-PCR approach and sequenced. Sequence data showed a single open reading frame coding for a protein of 180 amino acids with a signal sequence of 18 amino acid residues. Comparison with other ruminant β1g sequences revealed a high homology indicating the protein to be conserved through evolution. The degree of homology, at amino acid level, is 96.10/0, 95.6%, 93.9% and 63.7% with goat, sheep, cow and pig, respectively.
ISSN:1042-5179
1029-2365
DOI:10.3109/10425179909008426