Encapsulation of Hemoglobin in Non-Phospholipid Vesicles
The efficiency of encapsulating hemoglobin in non-phospholipid liposomes by rapidly mixing hemoglobin with lipids heated above their solidliquid phase transition temperature was examined. Human hemoglobin was mixed at 55-60°C with a lipid solution containing polyoxyethylene-2 cetyl ether and cholest...
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| Veröffentlicht in: | Artificial cells, blood substitutes, and immobilization biotechnology blood substitutes, and immobilization biotechnology, 1994, Vol.22 (3), p.849-854 |
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| container_title | Artificial cells, blood substitutes, and immobilization biotechnology |
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| creator | Vandegriff, K. D. Wallach, D. F. H. Winslow, R. M. |
| description | The efficiency of encapsulating hemoglobin in non-phospholipid liposomes by rapidly mixing hemoglobin with lipids heated above their solidliquid phase transition temperature was examined. Human hemoglobin was mixed at 55-60°C with a lipid solution containing polyoxyethylene-2 cetyl ether and cholesterol (molar ratio, 3:1) at 60-65°C. Repeated mixing was carried out through a high-shear orifice, followed by rapid cooling and additional mixing. Lipid vesicles were heterogeneous in size, with diameters from ∼300 nm to 10 μm. The non-encapsulated aqueous phase was removed by centrifugation, and total hemoglobin was determined spectrophotometrically. Encapsulation efficiency was calculated as the percentage of hemoglobin associated with the liposome phase (i.e., encapsulated) as a function of hemoglobin concentration and the aqueous:lipid hydration ratio. Hemoglobin concentrations were varied from 1 to 10 mM (in heme). Aqueous:lipid ratios of 8:1 and 4:1 were tested. Percent encapsulation varied from 13-30%, with the greatest efficiency, i.e., 30%, at a 4:1 hydration ratio of hemoglobin:lipid at 5.6 mM hemoglobin. |
| doi_str_mv | 10.3109/10731199409117920 |
| format | Article |
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Encapsulation efficiency was calculated as the percentage of hemoglobin associated with the liposome phase (i.e., encapsulated) as a function of hemoglobin concentration and the aqueous:lipid hydration ratio. Hemoglobin concentrations were varied from 1 to 10 mM (in heme). Aqueous:lipid ratios of 8:1 and 4:1 were tested. Percent encapsulation varied from 13-30%, with the greatest efficiency, i.e., 30%, at a 4:1 hydration ratio of hemoglobin:lipid at 5.6 mM hemoglobin.</description><identifier>ISSN: 1073-1199</identifier><identifier>EISSN: 1532-4184</identifier><identifier>DOI: 10.3109/10731199409117920</identifier><identifier>PMID: 7994408</identifier><identifier>CODEN: ABSBE4</identifier><language>eng</language><publisher>Monticello, NY: Informa UK Ltd</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. 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D.</creatorcontrib><creatorcontrib>Wallach, D. F. H.</creatorcontrib><creatorcontrib>Winslow, R. M.</creatorcontrib><title>Encapsulation of Hemoglobin in Non-Phospholipid Vesicles</title><title>Artificial cells, blood substitutes, and immobilization biotechnology</title><addtitle>Artif Cells Blood Substit Immobil Biotechnol</addtitle><description>The efficiency of encapsulating hemoglobin in non-phospholipid liposomes by rapidly mixing hemoglobin with lipids heated above their solidliquid phase transition temperature was examined. Human hemoglobin was mixed at 55-60°C with a lipid solution containing polyoxyethylene-2 cetyl ether and cholesterol (molar ratio, 3:1) at 60-65°C. Repeated mixing was carried out through a high-shear orifice, followed by rapid cooling and additional mixing. Lipid vesicles were heterogeneous in size, with diameters from ∼300 nm to 10 μm. The non-encapsulated aqueous phase was removed by centrifugation, and total hemoglobin was determined spectrophotometrically. Encapsulation efficiency was calculated as the percentage of hemoglobin associated with the liposome phase (i.e., encapsulated) as a function of hemoglobin concentration and the aqueous:lipid hydration ratio. Hemoglobin concentrations were varied from 1 to 10 mM (in heme). Aqueous:lipid ratios of 8:1 and 4:1 were tested. Percent encapsulation varied from 13-30%, with the greatest efficiency, i.e., 30%, at a 4:1 hydration ratio of hemoglobin:lipid at 5.6 mM hemoglobin.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Blood Substitutes - administration & dosage</subject><subject>Blood Substitutes - isolation & purification</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Cholesterol</subject><subject>Hemoglobins - administration & dosage</subject><subject>Hemoglobins - isolation & purification</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Liposomes</subject><subject>Medical sciences</subject><subject>Microspheres</subject><subject>Polyethylene Glycols</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Water</subject><issn>1073-1199</issn><issn>1532-4184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1L5TAQhoOsuH79AC8WzsXiXTWTpE3C7o2IXyDqhXpbpmm6J5I23aRF_PdGzlFYFhQCE3ifd5h5h5ADoEccqD4GKjmA1oJqAKkZ3SDbUHJWCFDiW_5nvXgDvpOdlJ4opVwA2yJbMnsEVdtEnQ0GxzR7nFwYFqFbXNo-_PGhccMiv5swFHfLkMZl8G507eLRJme8TXtks0Of7P667pKH87P708vi-vbi6vTkujCigqmQQrBSScVb1TYIlpW6apoSFaoWsOoMZ8KqlnUcDENBJUhZGSmAtp3WqPguOVz1HWP4O9s01b1LxnqPgw1zqmWlVCkYfAlCpbSWnGcQVqCJIaVou3qMrsf4UgOt32Kt_4s1e36sm89Nb9sPxzrHrP9c65gM-i7iYFz6wATLY3Kdsd8rzA1diD0-h-jbesIXH-K7h382xa9_7EuLfloajLZ-CnMc8h0-2eEV95Cizg</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Vandegriff, K. D.</creator><creator>Wallach, D. F. H.</creator><creator>Winslow, R. 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Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Blood Substitutes - administration & dosage</topic><topic>Blood Substitutes - isolation & purification</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Cholesterol</topic><topic>Hemoglobins - administration & dosage</topic><topic>Hemoglobins - isolation & purification</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Liposomes</topic><topic>Medical sciences</topic><topic>Microspheres</topic><topic>Polyethylene Glycols</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><topic>Water</topic><toplevel>online_resources</toplevel><creatorcontrib>Vandegriff, K. D.</creatorcontrib><creatorcontrib>Wallach, D. F. H.</creatorcontrib><creatorcontrib>Winslow, R. 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M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Encapsulation of Hemoglobin in Non-Phospholipid Vesicles</atitle><jtitle>Artificial cells, blood substitutes, and immobilization biotechnology</jtitle><addtitle>Artif Cells Blood Substit Immobil Biotechnol</addtitle><date>1994</date><risdate>1994</risdate><volume>22</volume><issue>3</issue><spage>849</spage><epage>854</epage><pages>849-854</pages><issn>1073-1199</issn><eissn>1532-4184</eissn><coden>ABSBE4</coden><abstract>The efficiency of encapsulating hemoglobin in non-phospholipid liposomes by rapidly mixing hemoglobin with lipids heated above their solidliquid phase transition temperature was examined. Human hemoglobin was mixed at 55-60°C with a lipid solution containing polyoxyethylene-2 cetyl ether and cholesterol (molar ratio, 3:1) at 60-65°C. Repeated mixing was carried out through a high-shear orifice, followed by rapid cooling and additional mixing. Lipid vesicles were heterogeneous in size, with diameters from ∼300 nm to 10 μm. The non-encapsulated aqueous phase was removed by centrifugation, and total hemoglobin was determined spectrophotometrically. Encapsulation efficiency was calculated as the percentage of hemoglobin associated with the liposome phase (i.e., encapsulated) as a function of hemoglobin concentration and the aqueous:lipid hydration ratio. Hemoglobin concentrations were varied from 1 to 10 mM (in heme). Aqueous:lipid ratios of 8:1 and 4:1 were tested. Percent encapsulation varied from 13-30%, with the greatest efficiency, i.e., 30%, at a 4:1 hydration ratio of hemoglobin:lipid at 5.6 mM hemoglobin.</abstract><cop>Monticello, NY</cop><pub>Informa UK Ltd</pub><pmid>7994408</pmid><doi>10.3109/10731199409117920</doi><tpages>6</tpages></addata></record> |
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| ispartof | Artificial cells, blood substitutes, and immobilization biotechnology, 1994, Vol.22 (3), p.849-854 |
| issn | 1073-1199 1532-4184 |
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| source | MEDLINE; Taylor & Francis Journals Complete |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Blood Substitutes - administration & dosage Blood Substitutes - isolation & purification Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Cholesterol Hemoglobins - administration & dosage Hemoglobins - isolation & purification Hot Temperature Humans In Vitro Techniques Liposomes Medical sciences Microspheres Polyethylene Glycols Transfusions. Complications. Transfusion reactions. Cell and gene therapy Water |
| title | Encapsulation of Hemoglobin in Non-Phospholipid Vesicles |
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