Radical Formation in the Rat Small Intestine During and Following Ischemia
Oxidative loading during the reperfusion of the proximal jejunum of rats following a one hour-period of complete ischemia was demonstrated in in vivo-experiments by the increases of the GSSG: total glutathione ratio and the concentration of TBA-RS. The pretreatment of the animals with the xanthine o...
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| Veröffentlicht in: | Free radical research 1989, Vol.7 (3-6), p.347-353 |
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| container_issue | 3-6 |
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| container_title | Free radical research |
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| creator | Siems, Werner Kowalewski, Jan Werner, Andreas Schimke, Ingolf Gerber, Gerhard |
| description | Oxidative loading during the reperfusion of the proximal jejunum of rats following a one hour-period of complete ischemia was demonstrated in in vivo-experiments by the increases of the GSSG: total glutathione ratio and the concentration of TBA-RS. The pretreatment of the animals with the xanthine oxidoreductase inhibitor allopurinol diminished the accumulation of GSSG and of TBA-RS. It was concluded that the purine nucleotide degradation is an important source of oxygen reduction products in reoxygenated small intestine. The tissue concentrations of nucleotides, nucleosides and nucleobases were measured by an ion-pair reversed-phase HPLC separation. There occurred fast declines of ATP and GTP concentrations during ischaemia leading to temporary increases of nucleoside mono- and diphosphate pools. The hypoxanthine concentration is increased about twenty fold during oxygen deficiency. The ATP and GTP restoration during the reperfusion was accelerated in presence of allopurinol. The shares of the beneficial allopurinol effects are not yet clarified. |
| doi_str_mv | 10.3109/10715768909087961 |
| format | Article |
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The pretreatment of the animals with the xanthine oxidoreductase inhibitor allopurinol diminished the accumulation of GSSG and of TBA-RS. It was concluded that the purine nucleotide degradation is an important source of oxygen reduction products in reoxygenated small intestine. The tissue concentrations of nucleotides, nucleosides and nucleobases were measured by an ion-pair reversed-phase HPLC separation. There occurred fast declines of ATP and GTP concentrations during ischaemia leading to temporary increases of nucleoside mono- and diphosphate pools. The hypoxanthine concentration is increased about twenty fold during oxygen deficiency. The ATP and GTP restoration during the reperfusion was accelerated in presence of allopurinol. 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The pretreatment of the animals with the xanthine oxidoreductase inhibitor allopurinol diminished the accumulation of GSSG and of TBA-RS. It was concluded that the purine nucleotide degradation is an important source of oxygen reduction products in reoxygenated small intestine. The tissue concentrations of nucleotides, nucleosides and nucleobases were measured by an ion-pair reversed-phase HPLC separation. There occurred fast declines of ATP and GTP concentrations during ischaemia leading to temporary increases of nucleoside mono- and diphosphate pools. The hypoxanthine concentration is increased about twenty fold during oxygen deficiency. The ATP and GTP restoration during the reperfusion was accelerated in presence of allopurinol. The shares of the beneficial allopurinol effects are not yet clarified.</description><subject>allopurinol</subject><subject>Animals</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Free Radicals</subject><subject>glutathione</subject><subject>Glutathione - metabolism</subject><subject>Intestine, Small - blood supply</subject><subject>Intestine, Small - metabolism</subject><subject>Ischemia - metabolism</subject><subject>Male</subject><subject>post ischemic damage</subject><subject>purine nucleotides</subject><subject>Purine Nucleotides - metabolism</subject><subject>rat small intestine</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Reperfusion</subject><subject>xanthine oxidoreductase</subject><issn>1071-5762</issn><issn>8755-0199</issn><issn>1029-2470</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9r3DAQxUVJSdO0H6CHgE-5udVYliWRXsLm34aFwqY9m1lZzirIUiLZhHz7yOxSCKU5jYZ57_H0I-Qb0O8MqPoBVAAXjVRUUSlUAx_IEdBKlVUt6MH8FlBmQfWJfE7pgVJgNReH5LDiknEOR-R2jZ3V6IqrEAccbfCF9cW4NcUax-JuQOeKpR9NGq03xcUUrb8v0HdZ71x4nrdl0lszWPxCPvbokvm6n8fkz9Xl78VNufp1vVycr0pdq2YsOd3UjEvs6tyxMkKYzUZzDVrohooOeykqwbBRogcJptNSAqdKigbylzvGjsnpLvcxhqcpN2sHm7RxDr0JU2qFYqxqoMpC2Al1DClF07eP0Q4YX1qg7cyv_Ydf9pzsw6fNYLq_jj2wfP-5u1vfz8SeQ3RdO-KLC7GP6LVNc_T_48_e2LcG3bjVGE37EKboM7d3yr0CGyGOoQ</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Siems, Werner</creator><creator>Kowalewski, Jan</creator><creator>Werner, Andreas</creator><creator>Schimke, Ingolf</creator><creator>Gerber, Gerhard</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Radical Formation in the Rat Small Intestine During and Following Ischemia</title><author>Siems, Werner ; Kowalewski, Jan ; Werner, Andreas ; Schimke, Ingolf ; Gerber, Gerhard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-50b4358ad44702e77ebbc5c1c7c607daf87273a697f181edc8815098761109d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>allopurinol</topic><topic>Animals</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Free Radicals</topic><topic>glutathione</topic><topic>Glutathione - metabolism</topic><topic>Intestine, Small - blood supply</topic><topic>Intestine, Small - metabolism</topic><topic>Ischemia - metabolism</topic><topic>Male</topic><topic>post ischemic damage</topic><topic>purine nucleotides</topic><topic>Purine Nucleotides - metabolism</topic><topic>rat small intestine</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reperfusion</topic><topic>xanthine oxidoreductase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siems, Werner</creatorcontrib><creatorcontrib>Kowalewski, Jan</creatorcontrib><creatorcontrib>Werner, Andreas</creatorcontrib><creatorcontrib>Schimke, Ingolf</creatorcontrib><creatorcontrib>Gerber, Gerhard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siems, Werner</au><au>Kowalewski, Jan</au><au>Werner, Andreas</au><au>Schimke, Ingolf</au><au>Gerber, Gerhard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radical Formation in the Rat Small Intestine During and Following Ischemia</atitle><jtitle>Free radical research</jtitle><addtitle>Free Radic Res Commun</addtitle><date>1989</date><risdate>1989</risdate><volume>7</volume><issue>3-6</issue><spage>347</spage><epage>353</epage><pages>347-353</pages><issn>1071-5762</issn><issn>8755-0199</issn><eissn>1029-2470</eissn><abstract>Oxidative loading during the reperfusion of the proximal jejunum of rats following a one hour-period of complete ischemia was demonstrated in in vivo-experiments by the increases of the GSSG: total glutathione ratio and the concentration of TBA-RS. The pretreatment of the animals with the xanthine oxidoreductase inhibitor allopurinol diminished the accumulation of GSSG and of TBA-RS. It was concluded that the purine nucleotide degradation is an important source of oxygen reduction products in reoxygenated small intestine. The tissue concentrations of nucleotides, nucleosides and nucleobases were measured by an ion-pair reversed-phase HPLC separation. There occurred fast declines of ATP and GTP concentrations during ischaemia leading to temporary increases of nucleoside mono- and diphosphate pools. The hypoxanthine concentration is increased about twenty fold during oxygen deficiency. The ATP and GTP restoration during the reperfusion was accelerated in presence of allopurinol. The shares of the beneficial allopurinol effects are not yet clarified.</abstract><cop>Switzerland</cop><pub>Informa UK Ltd</pub><pmid>2583551</pmid><doi>10.3109/10715768909087961</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1071-5762 |
| ispartof | Free radical research, 1989, Vol.7 (3-6), p.347-353 |
| issn | 1071-5762 8755-0199 1029-2470 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_3109_10715768909087961 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles) |
| subjects | allopurinol Animals Chromatography, High Pressure Liquid Free Radicals glutathione Glutathione - metabolism Intestine, Small - blood supply Intestine, Small - metabolism Ischemia - metabolism Male post ischemic damage purine nucleotides Purine Nucleotides - metabolism rat small intestine Rats Rats, Inbred Strains Reperfusion xanthine oxidoreductase |
| title | Radical Formation in the Rat Small Intestine During and Following Ischemia |
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