Polycystic ovary syndrome: evidence for reduced 3β-hydroxysteroid dehydrogenase gene expression in human luteinizing granulosa cells
In a preview study we found that luteinizing granulosa cells from follicles of patients with polycystic ovary syndrome (PCOS) have a reduced capacity to synthesize progesterone in vitro. Because the 3β -hydroxysteroid dehydrogenase/δ5-δ4-isomerase (3β -HSD) is an important enzyme for the biosynthesi...
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| Veröffentlicht in: | Gynecological endocrinology 2000, Vol.14 (1), p.32-37 |
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| creator | Doldi, N. Grossi, D. Destefani, A. Gessi, A. Ferrari, A. |
| description | In a preview study we found that luteinizing granulosa cells from follicles of patients with polycystic ovary syndrome (PCOS) have a reduced capacity to synthesize progesterone in vitro. Because the 3β -hydroxysteroid dehydrogenase/δ5-δ4-isomerase (3β -HSD) is an important enzyme for the biosynthesis of progesterone, the reduced capacity of PCO luteinizing granulosa cells to synthesize progesterone in vitro may be due to reduced 3β-HSD gene expression. A reverse transcriptase polymerase chain reaction for 3β -HSD was performed and the relative intensity of signals for 3β-HSD was evaluated using computer-assisted densitometry.
Cells from polycystic ovaries expressed less 3β -HSD in follicles ≤10 mm (p < 0.05) and in follicles ≥ 16 mm (p < 0.05) than cells from normal ovaries. Furthermore, after human chorionic gonadotropin stimulus (50 ng/ml), cells from polycystic ovaries expressed less 3 β-HSD in follicles ≥ 16 mm (p < 0.01) than cells from normal ovaries. The data show that there is a specific change in the gene expression of 3β-HSD in PCO granulosa cells resulting in a suppressed capacity to secrete progesterone. |
| doi_str_mv | 10.3109/09513590009167657 |
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Cells from polycystic ovaries expressed less 3β -HSD in follicles ≤10 mm (p < 0.05) and in follicles ≥ 16 mm (p < 0.05) than cells from normal ovaries. Furthermore, after human chorionic gonadotropin stimulus (50 ng/ml), cells from polycystic ovaries expressed less 3 β-HSD in follicles ≥ 16 mm (p < 0.01) than cells from normal ovaries. The data show that there is a specific change in the gene expression of 3β-HSD in PCO granulosa cells resulting in a suppressed capacity to secrete progesterone.</description><identifier>ISSN: 0951-3590</identifier><identifier>EISSN: 1473-0766</identifier><identifier>DOI: 10.3109/09513590009167657</identifier><identifier>PMID: 10813104</identifier><language>eng</language><publisher>Carnforth: Informa UK Ltd</publisher><subject>3-Hydroxysteroid Dehydrogenases - genetics ; 3β-Hydroxysteroid Dehydrogenase ; Biological and medical sciences ; Cells, Cultured ; Chorionic Gonadotropin - pharmacology ; Female ; Female genital diseases ; Gene Expression ; Granulosa Cells ; Granulosa Cells - drug effects ; Granulosa Cells - enzymology ; Gynecology. Andrology. Obstetrics ; Human Chorionic Gonadotropin ; Humans ; In Vitro Fertilization ; Medical sciences ; Non tumoral diseases ; Polycystic Ovary Syndrome ; Polycystic Ovary Syndrome - enzymology ; Progesterone ; Progesterone - secretion ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - analysis</subject><ispartof>Gynecological endocrinology, 2000, Vol.14 (1), p.32-37</ispartof><rights>2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2000</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-b9e5420ce8728a7e4922bff81794106f6fc1141a20d7ec8f58e03d6a01160a493</citedby><cites>FETCH-LOGICAL-c431t-b9e5420ce8728a7e4922bff81794106f6fc1141a20d7ec8f58e03d6a01160a493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/09513590009167657$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/09513590009167657$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,60436,61221,61402</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1452859$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10813104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doldi, N.</creatorcontrib><creatorcontrib>Grossi, D.</creatorcontrib><creatorcontrib>Destefani, A.</creatorcontrib><creatorcontrib>Gessi, A.</creatorcontrib><creatorcontrib>Ferrari, A.</creatorcontrib><title>Polycystic ovary syndrome: evidence for reduced 3β-hydroxysteroid dehydrogenase gene expression in human luteinizing granulosa cells</title><title>Gynecological endocrinology</title><addtitle>Gynecol Endocrinol</addtitle><description>In a preview study we found that luteinizing granulosa cells from follicles of patients with polycystic ovary syndrome (PCOS) have a reduced capacity to synthesize progesterone in vitro. Because the 3β -hydroxysteroid dehydrogenase/δ5-δ4-isomerase (3β -HSD) is an important enzyme for the biosynthesis of progesterone, the reduced capacity of PCO luteinizing granulosa cells to synthesize progesterone in vitro may be due to reduced 3β-HSD gene expression. A reverse transcriptase polymerase chain reaction for 3β -HSD was performed and the relative intensity of signals for 3β-HSD was evaluated using computer-assisted densitometry.
Cells from polycystic ovaries expressed less 3β -HSD in follicles ≤10 mm (p < 0.05) and in follicles ≥ 16 mm (p < 0.05) than cells from normal ovaries. Furthermore, after human chorionic gonadotropin stimulus (50 ng/ml), cells from polycystic ovaries expressed less 3 β-HSD in follicles ≥ 16 mm (p < 0.01) than cells from normal ovaries. The data show that there is a specific change in the gene expression of 3β-HSD in PCO granulosa cells resulting in a suppressed capacity to secrete progesterone.</description><subject>3-Hydroxysteroid Dehydrogenases - genetics</subject><subject>3β-Hydroxysteroid Dehydrogenase</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gene Expression</subject><subject>Granulosa Cells</subject><subject>Granulosa Cells - drug effects</subject><subject>Granulosa Cells - enzymology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Human Chorionic Gonadotropin</subject><subject>Humans</subject><subject>In Vitro Fertilization</subject><subject>Medical sciences</subject><subject>Non tumoral diseases</subject><subject>Polycystic Ovary Syndrome</subject><subject>Polycystic Ovary Syndrome - enzymology</subject><subject>Progesterone</subject><subject>Progesterone - secretion</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - analysis</subject><issn>0951-3590</issn><issn>1473-0766</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi1ERZfCA3BBPiBuKZ7YiRPoBVVAkSrBAc6R157sunLsxU5Kw50X4kF4Jhx2UUFIPY1kf_-vmY-QJ8BOObD2BWsr4FXLGGuhlnUl75EVCMkLJuv6Plkt_8UCHJOHKV0xBlzI8gE5BtZAbhAr8v1jcLOe02g1DdcqzjTN3sQw4EuK19ag10j7EGlEM2k0lP_8UWznTNzkEMZgDTX4-2GDXiWkeSDFm13ElGzw1Hq6nQblqZtGtN5-s35DN1H5yYWkqEbn0iNy1CuX8PFhnpDPb998Or8oLj-8e3_--rLQgsNYrFusRMk0NrJslETRluW67xuQrQBW93WvAQSokhmJuumrBhk3tWIANVOi5Sfk-b53F8OXCdPYDTYtGyiPYUqdhKy1qVgGYQ_qGFKK2He7aIespwPWLe67_9znzNND-bQe0PyV2MvOwLMDoJJWrs8OtE23nKjKplqWPNtj1mfxg_oaojPdqGYX4p8Mv2uNV__Et6jcuNUqYncVpuiz3zuO-AXFVbSd</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Doldi, N.</creator><creator>Grossi, D.</creator><creator>Destefani, A.</creator><creator>Gessi, A.</creator><creator>Ferrari, A.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Parthenon</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Polycystic ovary syndrome: evidence for reduced 3β-hydroxysteroid dehydrogenase gene expression in human luteinizing granulosa cells</title><author>Doldi, N. ; Grossi, D. ; Destefani, A. ; Gessi, A. ; Ferrari, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-b9e5420ce8728a7e4922bff81794106f6fc1141a20d7ec8f58e03d6a01160a493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>3-Hydroxysteroid Dehydrogenases - genetics</topic><topic>3β-Hydroxysteroid Dehydrogenase</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gene Expression</topic><topic>Granulosa Cells</topic><topic>Granulosa Cells - drug effects</topic><topic>Granulosa Cells - enzymology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Human Chorionic Gonadotropin</topic><topic>Humans</topic><topic>In Vitro Fertilization</topic><topic>Medical sciences</topic><topic>Non tumoral diseases</topic><topic>Polycystic Ovary Syndrome</topic><topic>Polycystic Ovary Syndrome - enzymology</topic><topic>Progesterone</topic><topic>Progesterone - secretion</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doldi, N.</creatorcontrib><creatorcontrib>Grossi, D.</creatorcontrib><creatorcontrib>Destefani, A.</creatorcontrib><creatorcontrib>Gessi, A.</creatorcontrib><creatorcontrib>Ferrari, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecological endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doldi, N.</au><au>Grossi, D.</au><au>Destefani, A.</au><au>Gessi, A.</au><au>Ferrari, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polycystic ovary syndrome: evidence for reduced 3β-hydroxysteroid dehydrogenase gene expression in human luteinizing granulosa cells</atitle><jtitle>Gynecological endocrinology</jtitle><addtitle>Gynecol Endocrinol</addtitle><date>2000</date><risdate>2000</risdate><volume>14</volume><issue>1</issue><spage>32</spage><epage>37</epage><pages>32-37</pages><issn>0951-3590</issn><eissn>1473-0766</eissn><abstract>In a preview study we found that luteinizing granulosa cells from follicles of patients with polycystic ovary syndrome (PCOS) have a reduced capacity to synthesize progesterone in vitro. Because the 3β -hydroxysteroid dehydrogenase/δ5-δ4-isomerase (3β -HSD) is an important enzyme for the biosynthesis of progesterone, the reduced capacity of PCO luteinizing granulosa cells to synthesize progesterone in vitro may be due to reduced 3β-HSD gene expression. A reverse transcriptase polymerase chain reaction for 3β -HSD was performed and the relative intensity of signals for 3β-HSD was evaluated using computer-assisted densitometry.
Cells from polycystic ovaries expressed less 3β -HSD in follicles ≤10 mm (p < 0.05) and in follicles ≥ 16 mm (p < 0.05) than cells from normal ovaries. Furthermore, after human chorionic gonadotropin stimulus (50 ng/ml), cells from polycystic ovaries expressed less 3 β-HSD in follicles ≥ 16 mm (p < 0.01) than cells from normal ovaries. The data show that there is a specific change in the gene expression of 3β-HSD in PCO granulosa cells resulting in a suppressed capacity to secrete progesterone.</abstract><cop>Carnforth</cop><pub>Informa UK Ltd</pub><pmid>10813104</pmid><doi>10.3109/09513590009167657</doi><tpages>6</tpages></addata></record> |
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| subjects | 3-Hydroxysteroid Dehydrogenases - genetics 3β-Hydroxysteroid Dehydrogenase Biological and medical sciences Cells, Cultured Chorionic Gonadotropin - pharmacology Female Female genital diseases Gene Expression Granulosa Cells Granulosa Cells - drug effects Granulosa Cells - enzymology Gynecology. Andrology. Obstetrics Human Chorionic Gonadotropin Humans In Vitro Fertilization Medical sciences Non tumoral diseases Polycystic Ovary Syndrome Polycystic Ovary Syndrome - enzymology Progesterone Progesterone - secretion Reverse Transcriptase Polymerase Chain Reaction RNA - analysis |
| title | Polycystic ovary syndrome: evidence for reduced 3β-hydroxysteroid dehydrogenase gene expression in human luteinizing granulosa cells |
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