Ultrastructural Characteristics of Central Neurocytoma in Cell Culture
The study was performed to determine the ultrastructural characteristics of central neurocytoma and its features in primary cell culture. Fresh tissue from three tumors was mechanically and enzymatically dissociated into individual cells, which were cultured onto poly-L-lysine precoated Aclar cover-...
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| Veröffentlicht in: | Ultrastructural pathology 1997, Vol.21 (4), p.393-404 |
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| container_title | Ultrastructural pathology |
| container_volume | 21 |
| creator | Lee, Min-Cheol Nam, Jong-Hee Choi, Chan Park, Chang-Soo Juhng, Sang-Woo Yang, Ki-Hwa Suh, Chae-Hong Kim, Seung U. Yang, Bok-Soog |
| description | The study was performed to determine the ultrastructural characteristics of central neurocytoma and its features in primary cell culture. Fresh tissue from three tumors was mechanically and enzymatically dissociated into individual cells, which were cultured onto poly-L-lysine precoated Aclar cover-slips in the media. The tumor cells attached to the surface of the coverslips within 12 to 24 h and delicate cytoplasmic processes developed within 2 to 3 days. Electron microscopic examination of the cultured tumor cells and the tumor tissue supported neuronal origin. Combined tissue culture and electron microscopic study provides a rapid, reliable, and reproducible means for the diagnosis of central neurocytoma. |
| doi_str_mv | 10.3109/01913129709021937 |
| format | Article |
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Fresh tissue from three tumors was mechanically and enzymatically dissociated into individual cells, which were cultured onto poly-L-lysine precoated Aclar cover-slips in the media. The tumor cells attached to the surface of the coverslips within 12 to 24 h and delicate cytoplasmic processes developed within 2 to 3 days. Electron microscopic examination of the cultured tumor cells and the tumor tissue supported neuronal origin. Combined tissue culture and electron microscopic study provides a rapid, reliable, and reproducible means for the diagnosis of central neurocytoma.</description><identifier>ISSN: 0191-3123</identifier><identifier>EISSN: 1521-0758</identifier><identifier>DOI: 10.3109/01913129709021937</identifier><identifier>PMID: 9206004</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Adolescent ; Adult ; Brain Neoplasms - therapy ; Brain Neoplasms - ultrastructure ; cell culture ; central neurocytoma ; Cerebral Ventricles - ultrastructure ; electron microscopy ; Female ; Humans ; Immunohistochemistry ; Magnetic Resonance Imaging ; Male ; Microscopy, Electron ; Neurocytoma - therapy ; Neurocytoma - ultrastructure ; Tumor Cells, Cultured</subject><ispartof>Ultrastructural pathology, 1997, Vol.21 (4), p.393-404</ispartof><rights>1997 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-f826f31037e17370a218163ae6691d1550412c1fbfb896df870004625c77a1013</citedby><cites>FETCH-LOGICAL-c401t-f826f31037e17370a218163ae6691d1550412c1fbfb896df870004625c77a1013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/01913129709021937$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/01913129709021937$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9206004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Min-Cheol</creatorcontrib><creatorcontrib>Nam, Jong-Hee</creatorcontrib><creatorcontrib>Choi, Chan</creatorcontrib><creatorcontrib>Park, Chang-Soo</creatorcontrib><creatorcontrib>Juhng, Sang-Woo</creatorcontrib><creatorcontrib>Yang, Ki-Hwa</creatorcontrib><creatorcontrib>Suh, Chae-Hong</creatorcontrib><creatorcontrib>Kim, Seung U.</creatorcontrib><creatorcontrib>Yang, Bok-Soog</creatorcontrib><title>Ultrastructural Characteristics of Central Neurocytoma in Cell Culture</title><title>Ultrastructural pathology</title><addtitle>Ultrastruct Pathol</addtitle><description>The study was performed to determine the ultrastructural characteristics of central neurocytoma and its features in primary cell culture. Fresh tissue from three tumors was mechanically and enzymatically dissociated into individual cells, which were cultured onto poly-L-lysine precoated Aclar cover-slips in the media. The tumor cells attached to the surface of the coverslips within 12 to 24 h and delicate cytoplasmic processes developed within 2 to 3 days. Electron microscopic examination of the cultured tumor cells and the tumor tissue supported neuronal origin. Combined tissue culture and electron microscopic study provides a rapid, reliable, and reproducible means for the diagnosis of central neurocytoma.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Brain Neoplasms - therapy</subject><subject>Brain Neoplasms - ultrastructure</subject><subject>cell culture</subject><subject>central neurocytoma</subject><subject>Cerebral Ventricles - ultrastructure</subject><subject>electron microscopy</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Neurocytoma - therapy</subject><subject>Neurocytoma - ultrastructure</subject><subject>Tumor Cells, Cultured</subject><issn>0191-3123</issn><issn>1521-0758</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LAzEQxYMotf75AB6EnrytziTdzQa9SLEqiF7seUnThK7sbnSSRfrtzdIiiOhpYN7vPR6PsTOES4GgrgAVCuRKggKOSsg9NsacYwYyL_fZeNCzBIhDdhTCGwDkAsoRGykOBcB0zOaLJpIOkXoTe9LNZLbWpE20VIdYmzDxbjKzXRykZ9uTN5voWz2pu_RuEt43yWdP2IHTTbCnu3vMFvO719lD9vRy_zi7fcrMFDBmruSFS82FtCiFBM2xxEJoWxQKV5jnMEVu0C3dslTFypUyVZ4WPDdSagQUx-xim_tO_qO3IVZtHUwqojvr-1BJBUoIPoC4BQ35EMi66p3qVtOmQqiG7apf2yXP-S68X7Z29e3YjZX0m61ed85Tqz89Nasq6k3jyZHuTB2G6L_jr3_Y11Y3cW002erN99Sl3f4p9wUOl40Z</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Lee, Min-Cheol</creator><creator>Nam, Jong-Hee</creator><creator>Choi, Chan</creator><creator>Park, Chang-Soo</creator><creator>Juhng, Sang-Woo</creator><creator>Yang, Ki-Hwa</creator><creator>Suh, Chae-Hong</creator><creator>Kim, Seung U.</creator><creator>Yang, Bok-Soog</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>Ultrastructural Characteristics of Central Neurocytoma in Cell Culture</title><author>Lee, Min-Cheol ; Nam, Jong-Hee ; Choi, Chan ; Park, Chang-Soo ; Juhng, Sang-Woo ; Yang, Ki-Hwa ; Suh, Chae-Hong ; Kim, Seung U. ; Yang, Bok-Soog</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-f826f31037e17370a218163ae6691d1550412c1fbfb896df870004625c77a1013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Brain Neoplasms - therapy</topic><topic>Brain Neoplasms - ultrastructure</topic><topic>cell culture</topic><topic>central neurocytoma</topic><topic>Cerebral Ventricles - ultrastructure</topic><topic>electron microscopy</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Magnetic Resonance Imaging</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Neurocytoma - therapy</topic><topic>Neurocytoma - ultrastructure</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Min-Cheol</creatorcontrib><creatorcontrib>Nam, Jong-Hee</creatorcontrib><creatorcontrib>Choi, Chan</creatorcontrib><creatorcontrib>Park, Chang-Soo</creatorcontrib><creatorcontrib>Juhng, Sang-Woo</creatorcontrib><creatorcontrib>Yang, Ki-Hwa</creatorcontrib><creatorcontrib>Suh, Chae-Hong</creatorcontrib><creatorcontrib>Kim, Seung U.</creatorcontrib><creatorcontrib>Yang, Bok-Soog</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ultrastructural pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Min-Cheol</au><au>Nam, Jong-Hee</au><au>Choi, Chan</au><au>Park, Chang-Soo</au><au>Juhng, Sang-Woo</au><au>Yang, Ki-Hwa</au><au>Suh, Chae-Hong</au><au>Kim, Seung U.</au><au>Yang, Bok-Soog</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural Characteristics of Central Neurocytoma in Cell Culture</atitle><jtitle>Ultrastructural pathology</jtitle><addtitle>Ultrastruct Pathol</addtitle><date>1997</date><risdate>1997</risdate><volume>21</volume><issue>4</issue><spage>393</spage><epage>404</epage><pages>393-404</pages><issn>0191-3123</issn><eissn>1521-0758</eissn><abstract>The study was performed to determine the ultrastructural characteristics of central neurocytoma and its features in primary cell culture. Fresh tissue from three tumors was mechanically and enzymatically dissociated into individual cells, which were cultured onto poly-L-lysine precoated Aclar cover-slips in the media. The tumor cells attached to the surface of the coverslips within 12 to 24 h and delicate cytoplasmic processes developed within 2 to 3 days. Electron microscopic examination of the cultured tumor cells and the tumor tissue supported neuronal origin. Combined tissue culture and electron microscopic study provides a rapid, reliable, and reproducible means for the diagnosis of central neurocytoma.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>9206004</pmid><doi>10.3109/01913129709021937</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0191-3123 |
| ispartof | Ultrastructural pathology, 1997, Vol.21 (4), p.393-404 |
| issn | 0191-3123 1521-0758 |
| language | eng |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Adolescent Adult Brain Neoplasms - therapy Brain Neoplasms - ultrastructure cell culture central neurocytoma Cerebral Ventricles - ultrastructure electron microscopy Female Humans Immunohistochemistry Magnetic Resonance Imaging Male Microscopy, Electron Neurocytoma - therapy Neurocytoma - ultrastructure Tumor Cells, Cultured |
| title | Ultrastructural Characteristics of Central Neurocytoma in Cell Culture |
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