Ontogeny of Rat Testicular Mitogenic Activity Studied with Transformed and Nontransformed Mouse 3T3 Fibroblasts
The ontogeny of the testicular mitogenic activity of Sprague Dawley rats was evaluated by stimulation in vitro of [3H]thymidine uptake and cell number in Swiss 3T3 and transformed NIH 3T3 fibroblasts. Addition of various amounts of testicular cytosolic protein resulted in a dose-dependent increase i...
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| Veröffentlicht in: | Archives of andrology 1990, Vol.24 (3), p.255-265 |
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| container_title | Archives of andrology |
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| creator | Searle, B. M. Pogach, L. M. Nathan, E. Von Hagen, J. Huang, H. F. S. Seebode, J. |
| description | The ontogeny of the testicular mitogenic activity of Sprague Dawley rats was evaluated by stimulation in vitro of [3H]thymidine uptake and cell number in Swiss 3T3 and transformed NIH 3T3 fibroblasts. Addition of various amounts of testicular cytosolic protein resulted in a dose-dependent increase in cell number in both cell lines. The [3H]thymidine responses to testicular cytosolic proteins was also dose dependent but in a nonparametric manner. In addition, the responses of 3T3 cells to testicular cytosol was apparently dependent on the developmental stage of the testis, the time of stimulation, and the proliferative nature of the fibroblast cell line. These results suggest the presence of multiple forms of mitogenic substance in testicular cytosol and imply that the relative amounts of these substances may depend on the age of the donor animal. These substances may be important in the proliferation or differentiation of cell types at various stages of testicular development. |
| doi_str_mv | 10.3109/01485019008987582 |
| format | Article |
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M. ; Pogach, L. M. ; Nathan, E. ; Von Hagen, J. ; Huang, H. F. S. ; Seebode, J.</creator><creatorcontrib>Searle, B. M. ; Pogach, L. M. ; Nathan, E. ; Von Hagen, J. ; Huang, H. F. S. ; Seebode, J.</creatorcontrib><description>The ontogeny of the testicular mitogenic activity of Sprague Dawley rats was evaluated by stimulation in vitro of [3H]thymidine uptake and cell number in Swiss 3T3 and transformed NIH 3T3 fibroblasts. Addition of various amounts of testicular cytosolic protein resulted in a dose-dependent increase in cell number in both cell lines. The [3H]thymidine responses to testicular cytosolic proteins was also dose dependent but in a nonparametric manner. In addition, the responses of 3T3 cells to testicular cytosol was apparently dependent on the developmental stage of the testis, the time of stimulation, and the proliferative nature of the fibroblast cell line. These results suggest the presence of multiple forms of mitogenic substance in testicular cytosol and imply that the relative amounts of these substances may depend on the age of the donor animal. 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M.</creatorcontrib><creatorcontrib>Pogach, L. M.</creatorcontrib><creatorcontrib>Nathan, E.</creatorcontrib><creatorcontrib>Von Hagen, J.</creatorcontrib><creatorcontrib>Huang, H. F. S.</creatorcontrib><creatorcontrib>Seebode, J.</creatorcontrib><title>Ontogeny of Rat Testicular Mitogenic Activity Studied with Transformed and Nontransformed Mouse 3T3 Fibroblasts</title><title>Archives of andrology</title><addtitle>Arch Androl</addtitle><description>The ontogeny of the testicular mitogenic activity of Sprague Dawley rats was evaluated by stimulation in vitro of [3H]thymidine uptake and cell number in Swiss 3T3 and transformed NIH 3T3 fibroblasts. Addition of various amounts of testicular cytosolic protein resulted in a dose-dependent increase in cell number in both cell lines. The [3H]thymidine responses to testicular cytosolic proteins was also dose dependent but in a nonparametric manner. In addition, the responses of 3T3 cells to testicular cytosol was apparently dependent on the developmental stage of the testis, the time of stimulation, and the proliferative nature of the fibroblast cell line. These results suggest the presence of multiple forms of mitogenic substance in testicular cytosol and imply that the relative amounts of these substances may depend on the age of the donor animal. These substances may be important in the proliferation or differentiation of cell types at various stages of testicular development.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Cell Division - drug effects</subject><subject>Cell Line</subject><subject>Cell Line, Transformed</subject><subject>Cytosol - metabolism</subject><subject>Fibroblast</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Male</subject><subject>Mice</subject><subject>Mitogenic</subject><subject>Mitogens</subject><subject>Mouse</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Testis</subject><subject>Testis - growth & development</subject><subject>Testis - metabolism</subject><subject>Time Factors</subject><subject>Tissue Extracts - pharmacology</subject><issn>0148-5016</issn><issn>1521-0375</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFtLwzAUx4Moc04_gA9Cnnyr5tK0KfoyxBs4BzqfS5omLqNrZpI69u3N3BBF9OnA-V845wfAMUZnFKPiHOGUM4QLhHjBc8bJDuhjRnCCaM52QX-tJ9GQ7YMD72cIIcIy1AM9QhnladEHdtwG-6raFbQaPokAJ8oHI7tGODgyn5KRcCiDeTdhBZ9DVxtVw6UJUzhxovXaunlciLaGj7YN31Yj23kF6YTCG1M5WzXCB38I9rRovDrazgF4ubmeXN0lD-Pb-6vhQyJTQkOSsiytmZA6xTnRBBVSZxWtuKqYqnUtVJ0WqYzfCaURJgXNM4YVxyLjFOuqogNwuuldOPvWxZ_KufFSNY1oVbyrzAtOssgsGvHGKJ313ildLpyZC7cqMSrXkMtfkGPmZFveVfHTr8SWatQvN7pp1yzE0rqmLoNYNdbpSEgav67-u_7iR3yqRBOmUjhVzmzn2sjtn-M-AIPgnkY</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Searle, B. M.</creator><creator>Pogach, L. M.</creator><creator>Nathan, E.</creator><creator>Von Hagen, J.</creator><creator>Huang, H. F. S.</creator><creator>Seebode, J.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Ontogeny of Rat Testicular Mitogenic Activity Studied with Transformed and Nontransformed Mouse 3T3 Fibroblasts</title><author>Searle, B. M. ; Pogach, L. M. ; Nathan, E. ; Von Hagen, J. ; Huang, H. F. 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M.</creatorcontrib><creatorcontrib>Pogach, L. M.</creatorcontrib><creatorcontrib>Nathan, E.</creatorcontrib><creatorcontrib>Von Hagen, J.</creatorcontrib><creatorcontrib>Huang, H. F. S.</creatorcontrib><creatorcontrib>Seebode, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Searle, B. M.</au><au>Pogach, L. M.</au><au>Nathan, E.</au><au>Von Hagen, J.</au><au>Huang, H. F. 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| fulltext | fulltext |
| identifier | ISSN: 0148-5016 |
| ispartof | Archives of andrology, 1990, Vol.24 (3), p.255-265 |
| issn | 0148-5016 1521-0375 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_3109_01485019008987582 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Aging - metabolism Animals Cell Division - drug effects Cell Line Cell Line, Transformed Cytosol - metabolism Fibroblast Fibroblasts - cytology Fibroblasts - drug effects Male Mice Mitogenic Mitogens Mouse Rat Rats Rats, Inbred Strains Testis Testis - growth & development Testis - metabolism Time Factors Tissue Extracts - pharmacology |
| title | Ontogeny of Rat Testicular Mitogenic Activity Studied with Transformed and Nontransformed Mouse 3T3 Fibroblasts |
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