OXYMETHOLONE MODULATES CELL-MEDIATED IMMUNITY IN MALE B6C3F1 MICE
Oxymetholone is a synthetic androgen, structurally related to testosterone. It is currently used to treat anemias, but has also been abused as a performance enhancing anabolic steroid by the sport community. Concern about its suspected immunomodulatory properties provided the incentive for a detaile...
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| Veröffentlicht in: | Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2000-01, Vol.23 (4), p.621-644 |
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| container_title | Drug and chemical toxicology (New York, N.Y. 1978) |
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| creator | Karrow, N. A. McCay, J. A. Brown, R. Musgrove, D. Munson, A. E. White, K. L. White, Kimbler L. |
| description | Oxymetholone is a synthetic androgen, structurally related to testosterone. It is currently used to treat anemias, but has also been abused as a performance enhancing anabolic steroid by the sport community. Concern about its suspected immunomodulatory properties provided the incentive for a detailed investigation into its effects on the mammalian immune system. In this study, male B6C3F1 mice were treated for 14 d with oxymetholone (0, 50, 150, and 300 mg kg) by gastric intubation, then evaluated for immunotoxicity using a panel of immunotoxicity assays. Except for an increasing trend in kidney and liver weights, and a dose-dependent increase in serum blood urea nitrogen levels, no other signs of systemic toxicity were observed. Bone marrow DNA synthesis was reduced, though this did not translate into alterations in myeloid or monocyte colony forming units. Spleen B and T cell numbers, antibody response to sheep red blood cells, proliferative response to both mitogen and immunoglobulin receptor immunogens, and NK cell activity were all unaltered in mice treated with oxymetholone. Peritoneal macrophage activity was also unaffected by oxymetholone treatment. A 38% decrease in the spleen cell mixed leukocyte response, and a 15% decrease in cytotoxic T cell activity, measured in the highest oxymetholone treatment group, indicate that cell-mediated immunity was impaired following exposure. This immunomodulation did not however, translate into a change in host resistance to Listeria monocytogenes. |
| doi_str_mv | 10.1081/DCT-100101974 |
| format | Article |
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A. ; McCay, J. A. ; Brown, R. ; Musgrove, D. ; Munson, A. E. ; White, K. L. ; White, Kimbler L.</creator><creatorcontrib>Karrow, N. A. ; McCay, J. A. ; Brown, R. ; Musgrove, D. ; Munson, A. E. ; White, K. L. ; White, Kimbler L.</creatorcontrib><description>Oxymetholone is a synthetic androgen, structurally related to testosterone. It is currently used to treat anemias, but has also been abused as a performance enhancing anabolic steroid by the sport community. Concern about its suspected immunomodulatory properties provided the incentive for a detailed investigation into its effects on the mammalian immune system. In this study, male B6C3F1 mice were treated for 14 d with oxymetholone (0, 50, 150, and 300 mg kg) by gastric intubation, then evaluated for immunotoxicity using a panel of immunotoxicity assays. Except for an increasing trend in kidney and liver weights, and a dose-dependent increase in serum blood urea nitrogen levels, no other signs of systemic toxicity were observed. Bone marrow DNA synthesis was reduced, though this did not translate into alterations in myeloid or monocyte colony forming units. Spleen B and T cell numbers, antibody response to sheep red blood cells, proliferative response to both mitogen and immunoglobulin receptor immunogens, and NK cell activity were all unaltered in mice treated with oxymetholone. Peritoneal macrophage activity was also unaffected by oxymetholone treatment. A 38% decrease in the spleen cell mixed leukocyte response, and a 15% decrease in cytotoxic T cell activity, measured in the highest oxymetholone treatment group, indicate that cell-mediated immunity was impaired following exposure. This immunomodulation did not however, translate into a change in host resistance to Listeria monocytogenes.</description><identifier>ISSN: 0148-0545</identifier><identifier>EISSN: 1525-6014</identifier><identifier>DOI: 10.1081/DCT-100101974</identifier><identifier>PMID: 11071398</identifier><language>eng</language><publisher>New York, NY: Informa UK Ltd</publisher><subject>Anabolic Agents - toxicity ; Animals ; B-Lymphocytes - drug effects ; B-Lymphocytes - metabolism ; Biological and medical sciences ; Blood Chemical Analysis ; Bone Marrow Cells - cytology ; Cell Division - drug effects ; Cell-mediated immunity ; Cyclophosphamide - pharmacology ; Drug toxicity and drugs side effects treatment ; Hemoglobins - analysis ; Humans ; Immunity, Cellular - drug effects ; Immunity, Innate - drug effects ; Immunity, Innate - immunology ; Immunoglobulin M - drug effects ; Immunoglobulin M - metabolism ; Immunomodulation ; Immunosuppressive Agents - pharmacology ; Killer Cells, Natural - drug effects ; Killer Cells, Natural - metabolism ; Listeria monocytogenes ; Listeria monocytogenes - immunology ; Listeria monocytogenes - metabolism ; Lymphocyte Culture Test, Mixed ; Macrophages, Peritoneal - drug effects ; Macrophages, Peritoneal - metabolism ; Male ; Medical sciences ; Mice ; Mice, Inbred DBA ; Miscellaneous (drug allergy, mutagens, teratogens...) ; Oxymetholone ; Oxymetholone - toxicity ; Pharmacology. 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A.</creatorcontrib><creatorcontrib>McCay, J. A.</creatorcontrib><creatorcontrib>Brown, R.</creatorcontrib><creatorcontrib>Musgrove, D.</creatorcontrib><creatorcontrib>Munson, A. E.</creatorcontrib><creatorcontrib>White, K. L.</creatorcontrib><creatorcontrib>White, Kimbler L.</creatorcontrib><title>OXYMETHOLONE MODULATES CELL-MEDIATED IMMUNITY IN MALE B6C3F1 MICE</title><title>Drug and chemical toxicology (New York, N.Y. 1978)</title><addtitle>Drug Chem Toxicol</addtitle><description>Oxymetholone is a synthetic androgen, structurally related to testosterone. It is currently used to treat anemias, but has also been abused as a performance enhancing anabolic steroid by the sport community. Concern about its suspected immunomodulatory properties provided the incentive for a detailed investigation into its effects on the mammalian immune system. In this study, male B6C3F1 mice were treated for 14 d with oxymetholone (0, 50, 150, and 300 mg kg) by gastric intubation, then evaluated for immunotoxicity using a panel of immunotoxicity assays. Except for an increasing trend in kidney and liver weights, and a dose-dependent increase in serum blood urea nitrogen levels, no other signs of systemic toxicity were observed. Bone marrow DNA synthesis was reduced, though this did not translate into alterations in myeloid or monocyte colony forming units. Spleen B and T cell numbers, antibody response to sheep red blood cells, proliferative response to both mitogen and immunoglobulin receptor immunogens, and NK cell activity were all unaltered in mice treated with oxymetholone. Peritoneal macrophage activity was also unaffected by oxymetholone treatment. A 38% decrease in the spleen cell mixed leukocyte response, and a 15% decrease in cytotoxic T cell activity, measured in the highest oxymetholone treatment group, indicate that cell-mediated immunity was impaired following exposure. This immunomodulation did not however, translate into a change in host resistance to Listeria monocytogenes.</description><subject>Anabolic Agents - toxicity</subject><subject>Animals</subject><subject>B-Lymphocytes - drug effects</subject><subject>B-Lymphocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blood Chemical Analysis</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cell Division - drug effects</subject><subject>Cell-mediated immunity</subject><subject>Cyclophosphamide - pharmacology</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Hemoglobins - analysis</subject><subject>Humans</subject><subject>Immunity, Cellular - drug effects</subject><subject>Immunity, Innate - drug effects</subject><subject>Immunity, Innate - immunology</subject><subject>Immunoglobulin M - drug effects</subject><subject>Immunoglobulin M - metabolism</subject><subject>Immunomodulation</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Killer Cells, Natural - drug effects</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - immunology</subject><subject>Listeria monocytogenes - metabolism</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Macrophages, Peritoneal - drug effects</subject><subject>Macrophages, Peritoneal - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred DBA</subject><subject>Miscellaneous (drug allergy, mutagens, teratogens...)</subject><subject>Oxymetholone</subject><subject>Oxymetholone - toxicity</subject><subject>Pharmacology. Drug treatments</subject><subject>Random Allocation</subject><subject>Spleen - cytology</subject><subject>Spleen - drug effects</subject><subject>Spleen - metabolism</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - metabolism</subject><issn>0148-0545</issn><issn>1525-6014</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10M1LwzAYBvAgis6Po1cpCN6qeZumSY6zq67Qrgc7cKeQpSlOulWTDdl_b2RT8SCBhMDvfXl4ELoEfAuYw90orUPAGDAIFh-gAdCIhgmG-BAN_M1DTGN6gk6de_UqEpQcoxMAzIAIPkDD6nlWZvW4KqpJFpTVaFoM6-wpSLOiCMtslPvfKMjLcjrJ61mQT4JyWGTBfZKSBwjKPM3O0VGrOmcu9u8Zmj5kdToOi-oxT4dFqOOYrsM5QMJYA3PMIsO1brjyKTTVkRBkTpoEEsFM1DZUK8wTqrhmIjKUC3-imJAzdLPb-2b7941xa7lcOG26Tq1Mv3ESGKOMMOFhuIPa9s5Z08o3u1gqu5WA5Vdn0ncmfzrz_mq_eDNfmuZX70vy4HoPlNOqa61a6YX7cYyLOEm84ju1WLW9XaqP3naNXKtt19vvEfJfAvZn9MWobv2ilTXytd_Yla_1n-yfUYqPiA</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Karrow, N. 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Drug treatments</topic><topic>Random Allocation</topic><topic>Spleen - cytology</topic><topic>Spleen - drug effects</topic><topic>Spleen - metabolism</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karrow, N. A.</creatorcontrib><creatorcontrib>McCay, J. A.</creatorcontrib><creatorcontrib>Brown, R.</creatorcontrib><creatorcontrib>Musgrove, D.</creatorcontrib><creatorcontrib>Munson, A. E.</creatorcontrib><creatorcontrib>White, K. 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L.</au><au>White, Kimbler L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OXYMETHOLONE MODULATES CELL-MEDIATED IMMUNITY IN MALE B6C3F1 MICE</atitle><jtitle>Drug and chemical toxicology (New York, N.Y. 1978)</jtitle><addtitle>Drug Chem Toxicol</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>23</volume><issue>4</issue><spage>621</spage><epage>644</epage><pages>621-644</pages><issn>0148-0545</issn><eissn>1525-6014</eissn><abstract>Oxymetholone is a synthetic androgen, structurally related to testosterone. It is currently used to treat anemias, but has also been abused as a performance enhancing anabolic steroid by the sport community. Concern about its suspected immunomodulatory properties provided the incentive for a detailed investigation into its effects on the mammalian immune system. In this study, male B6C3F1 mice were treated for 14 d with oxymetholone (0, 50, 150, and 300 mg kg) by gastric intubation, then evaluated for immunotoxicity using a panel of immunotoxicity assays. Except for an increasing trend in kidney and liver weights, and a dose-dependent increase in serum blood urea nitrogen levels, no other signs of systemic toxicity were observed. Bone marrow DNA synthesis was reduced, though this did not translate into alterations in myeloid or monocyte colony forming units. Spleen B and T cell numbers, antibody response to sheep red blood cells, proliferative response to both mitogen and immunoglobulin receptor immunogens, and NK cell activity were all unaltered in mice treated with oxymetholone. Peritoneal macrophage activity was also unaffected by oxymetholone treatment. A 38% decrease in the spleen cell mixed leukocyte response, and a 15% decrease in cytotoxic T cell activity, measured in the highest oxymetholone treatment group, indicate that cell-mediated immunity was impaired following exposure. This immunomodulation did not however, translate into a change in host resistance to Listeria monocytogenes.</abstract><cop>New York, NY</cop><pub>Informa UK Ltd</pub><pmid>11071398</pmid><doi>10.1081/DCT-100101974</doi><tpages>24</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0148-0545 |
| ispartof | Drug and chemical toxicology (New York, N.Y. 1978), 2000-01, Vol.23 (4), p.621-644 |
| issn | 0148-0545 1525-6014 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_1081_DCT_100101974 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Access via Taylor & Francis |
| subjects | Anabolic Agents - toxicity Animals B-Lymphocytes - drug effects B-Lymphocytes - metabolism Biological and medical sciences Blood Chemical Analysis Bone Marrow Cells - cytology Cell Division - drug effects Cell-mediated immunity Cyclophosphamide - pharmacology Drug toxicity and drugs side effects treatment Hemoglobins - analysis Humans Immunity, Cellular - drug effects Immunity, Innate - drug effects Immunity, Innate - immunology Immunoglobulin M - drug effects Immunoglobulin M - metabolism Immunomodulation Immunosuppressive Agents - pharmacology Killer Cells, Natural - drug effects Killer Cells, Natural - metabolism Listeria monocytogenes Listeria monocytogenes - immunology Listeria monocytogenes - metabolism Lymphocyte Culture Test, Mixed Macrophages, Peritoneal - drug effects Macrophages, Peritoneal - metabolism Male Medical sciences Mice Mice, Inbred DBA Miscellaneous (drug allergy, mutagens, teratogens...) Oxymetholone Oxymetholone - toxicity Pharmacology. Drug treatments Random Allocation Spleen - cytology Spleen - drug effects Spleen - metabolism T-Lymphocytes - drug effects T-Lymphocytes - metabolism |
| title | OXYMETHOLONE MODULATES CELL-MEDIATED IMMUNITY IN MALE B6C3F1 MICE |
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