Prime-boost Vaccination based on DNA and Protein-loaded Microspheres for Tuberculosis Prevention
We evaluated the use of a vaccine formulation based on a mixture of two different PLGA microspheres, composed by faster and slower release profiles, containing DNA encoding hsp65 and the recombinant hsp65 protein, respectively, aiming to DNA priming and protein boost after a single dose vaccination....
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| Veröffentlicht in: | Journal of drug targeting 2004-05, Vol.12 (4), p.195-203 |
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| container_title | Journal of drug targeting |
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| creator | Ruberti, Maristela Lima, Karla de Melo dos Santos, Sandra Aparecida Brandao, Izaira Tincani Soares, Edson Garcia Silva, Célio Lopes Rodrigues Júnior, José Maciel |
| description | We evaluated the use of a vaccine formulation based on a mixture of two different PLGA microspheres, composed by faster and slower release profiles, containing DNA encoding hsp65 and the recombinant hsp65 protein, respectively, aiming to DNA priming and protein boost after a single dose vaccination. The combination of PLGA50:50 microspheres containing DNA-hsp65 and trehalose dimycolate (TDM) with PLGA75:25 microspheres containing recombinant hsp65 (prime-boost Me) was able to induce high levels of anti-hsp65 specific antibodies. The serum levels of these specific antibodies remained high during 90 days after vaccination, whereas the DNA Me formulation based only in DNA-hsp65 plus TDM-loaded microspheres was not able to sustain the high antibody levels during the same period. Production of IFN- was significant in animals vaccinated with both formulations, while the prime-boost Me vaccinated mice sustained higher levels of this cytokine during all the evaluation period. Thus, prime-boost strategy by using biodegradable microspheres seems to be a promising strategy to stimulate long-lasting immune response. |
| doi_str_mv | 10.1080/10611860410001723126 |
| format | Article |
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The combination of PLGA50:50 microspheres containing DNA-hsp65 and trehalose dimycolate (TDM) with PLGA75:25 microspheres containing recombinant hsp65 (prime-boost Me) was able to induce high levels of anti-hsp65 specific antibodies. The serum levels of these specific antibodies remained high during 90 days after vaccination, whereas the DNA Me formulation based only in DNA-hsp65 plus TDM-loaded microspheres was not able to sustain the high antibody levels during the same period. Production of IFN- was significant in animals vaccinated with both formulations, while the prime-boost Me vaccinated mice sustained higher levels of this cytokine during all the evaluation period. Thus, prime-boost strategy by using biodegradable microspheres seems to be a promising strategy to stimulate long-lasting immune response.</description><identifier>ISSN: 1061-186X</identifier><identifier>EISSN: 1029-2330</identifier><identifier>DOI: 10.1080/10611860410001723126</identifier><identifier>PMID: 15506168</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Antibodies, Bacterial - blood ; Bacterial Proteins - administration & dosage ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Chaperonin 60 ; Chaperonins - administration & dosage ; Chaperonins - genetics ; Chaperonins - immunology ; Cytokines - blood ; DNA vaccines ; Hsp65 ; Immunization, Secondary - methods ; Interferon-gamma - biosynthesis ; Lactic Acid - chemistry ; Lung - pathology ; Mice ; Mice, Inbred BALB C ; Microspheres ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - pathogenicity ; Particle Size ; PLGA microspheres ; Polyglycolic Acid - chemistry ; Polymers - chemistry ; Prime boost ; Recombinant Proteins - administration & dosage ; Recombinant Proteins - immunology ; Spleen - metabolism ; Time Factors ; Tuberculosis ; Tuberculosis, Pulmonary - immunology ; Tuberculosis, Pulmonary - microbiology ; Tuberculosis, Pulmonary - prevention & control ; Vaccines, DNA - administration & dosage</subject><ispartof>Journal of drug targeting, 2004-05, Vol.12 (4), p.195-203</ispartof><rights>2004 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-c5576de22c184d89f8298e48a3bd04c46a0f591145e0363b9fecdc0dbe16e2a13</citedby><cites>FETCH-LOGICAL-c445t-c5576de22c184d89f8298e48a3bd04c46a0f591145e0363b9fecdc0dbe16e2a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/10611860410001723126$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/10611860410001723126$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15506168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruberti, Maristela</creatorcontrib><creatorcontrib>Lima, Karla de Melo</creatorcontrib><creatorcontrib>dos Santos, Sandra Aparecida</creatorcontrib><creatorcontrib>Brandao, Izaira Tincani</creatorcontrib><creatorcontrib>Soares, Edson Garcia</creatorcontrib><creatorcontrib>Silva, Célio Lopes</creatorcontrib><creatorcontrib>Rodrigues Júnior, José Maciel</creatorcontrib><title>Prime-boost Vaccination based on DNA and Protein-loaded Microspheres for Tuberculosis Prevention</title><title>Journal of drug targeting</title><addtitle>J Drug Target</addtitle><description>We evaluated the use of a vaccine formulation based on a mixture of two different PLGA microspheres, composed by faster and slower release profiles, containing DNA encoding hsp65 and the recombinant hsp65 protein, respectively, aiming to DNA priming and protein boost after a single dose vaccination. The combination of PLGA50:50 microspheres containing DNA-hsp65 and trehalose dimycolate (TDM) with PLGA75:25 microspheres containing recombinant hsp65 (prime-boost Me) was able to induce high levels of anti-hsp65 specific antibodies. The serum levels of these specific antibodies remained high during 90 days after vaccination, whereas the DNA Me formulation based only in DNA-hsp65 plus TDM-loaded microspheres was not able to sustain the high antibody levels during the same period. Production of IFN- was significant in animals vaccinated with both formulations, while the prime-boost Me vaccinated mice sustained higher levels of this cytokine during all the evaluation period. Thus, prime-boost strategy by using biodegradable microspheres seems to be a promising strategy to stimulate long-lasting immune response.</description><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bacterial Proteins - administration & dosage</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - immunology</subject><subject>Chaperonin 60</subject><subject>Chaperonins - administration & dosage</subject><subject>Chaperonins - genetics</subject><subject>Chaperonins - immunology</subject><subject>Cytokines - blood</subject><subject>DNA vaccines</subject><subject>Hsp65</subject><subject>Immunization, Secondary - methods</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Lactic Acid - chemistry</subject><subject>Lung - pathology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microspheres</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - pathogenicity</subject><subject>Particle Size</subject><subject>PLGA microspheres</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Polymers - chemistry</subject><subject>Prime boost</subject><subject>Recombinant Proteins - administration & dosage</subject><subject>Recombinant Proteins - immunology</subject><subject>Spleen - metabolism</subject><subject>Time Factors</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Pulmonary - immunology</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><subject>Tuberculosis, Pulmonary - prevention & control</subject><subject>Vaccines, DNA - administration & dosage</subject><issn>1061-186X</issn><issn>1029-2330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7of-A5E-eWutStKZ9EVZVl2FVfewireYTqqZLD3JbNKt7L83wwyIIO4pBfXUm-Qpxp4hvETQ8ApBIWoFEgEAV1wgVw_YMQLvWy4EPNzVCtvKfD9iJ6XcVEwohMfsCLuutpQ-Zj-ucthQO6RU5uabdS5EO4cUm8EW8k0t3n4-a2z0zVVOM4XYTsn62vkUXE5lu6ZMpRlTbq6XgbJbplRCqTD9pLgLesIejXYq9PRwnrKv799dn39oL79cfDw_u2ydlN3cuq5bKU-cO9TS637UvNcktRWDB-mksjB2PaLsCIQSQz-S8w78QKiIWxSn7MU-d5vT7UJlNptQHE2TjZSWYtQKqih9P4gaZAd8VUG5B3cfLZlGs62ubL4zCGa3AvOvFdSx54f8ZdiQ_zN0cF6BN3sgxOptY3-lPHkz27sp5THb6EIx4p4rXv-VsCY7zWtnM5mbtORYPf__jb8BI3ynzQ</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Ruberti, Maristela</creator><creator>Lima, Karla de Melo</creator><creator>dos Santos, Sandra Aparecida</creator><creator>Brandao, Izaira Tincani</creator><creator>Soares, Edson Garcia</creator><creator>Silva, Célio Lopes</creator><creator>Rodrigues Júnior, José Maciel</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>Prime-boost Vaccination based on DNA and Protein-loaded Microspheres for Tuberculosis Prevention</title><author>Ruberti, Maristela ; Lima, Karla de Melo ; dos Santos, Sandra Aparecida ; Brandao, Izaira Tincani ; Soares, Edson Garcia ; Silva, Célio Lopes ; Rodrigues Júnior, José Maciel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-c5576de22c184d89f8298e48a3bd04c46a0f591145e0363b9fecdc0dbe16e2a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Bacterial Proteins - administration & dosage</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - immunology</topic><topic>Chaperonin 60</topic><topic>Chaperonins - administration & dosage</topic><topic>Chaperonins - genetics</topic><topic>Chaperonins - immunology</topic><topic>Cytokines - blood</topic><topic>DNA vaccines</topic><topic>Hsp65</topic><topic>Immunization, Secondary - methods</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Lactic Acid - chemistry</topic><topic>Lung - pathology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microspheres</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - pathogenicity</topic><topic>Particle Size</topic><topic>PLGA microspheres</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polymers - chemistry</topic><topic>Prime boost</topic><topic>Recombinant Proteins - administration & dosage</topic><topic>Recombinant Proteins - immunology</topic><topic>Spleen - metabolism</topic><topic>Time Factors</topic><topic>Tuberculosis</topic><topic>Tuberculosis, Pulmonary - immunology</topic><topic>Tuberculosis, Pulmonary - microbiology</topic><topic>Tuberculosis, Pulmonary - prevention & control</topic><topic>Vaccines, DNA - administration & dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruberti, Maristela</creatorcontrib><creatorcontrib>Lima, Karla de Melo</creatorcontrib><creatorcontrib>dos Santos, Sandra Aparecida</creatorcontrib><creatorcontrib>Brandao, Izaira Tincani</creatorcontrib><creatorcontrib>Soares, Edson Garcia</creatorcontrib><creatorcontrib>Silva, Célio Lopes</creatorcontrib><creatorcontrib>Rodrigues Júnior, José Maciel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of drug targeting</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruberti, Maristela</au><au>Lima, Karla de Melo</au><au>dos Santos, Sandra Aparecida</au><au>Brandao, Izaira Tincani</au><au>Soares, Edson Garcia</au><au>Silva, Célio Lopes</au><au>Rodrigues Júnior, José Maciel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prime-boost Vaccination based on DNA and Protein-loaded Microspheres for Tuberculosis Prevention</atitle><jtitle>Journal of drug targeting</jtitle><addtitle>J Drug Target</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>12</volume><issue>4</issue><spage>195</spage><epage>203</epage><pages>195-203</pages><issn>1061-186X</issn><eissn>1029-2330</eissn><abstract>We evaluated the use of a vaccine formulation based on a mixture of two different PLGA microspheres, composed by faster and slower release profiles, containing DNA encoding hsp65 and the recombinant hsp65 protein, respectively, aiming to DNA priming and protein boost after a single dose vaccination. The combination of PLGA50:50 microspheres containing DNA-hsp65 and trehalose dimycolate (TDM) with PLGA75:25 microspheres containing recombinant hsp65 (prime-boost Me) was able to induce high levels of anti-hsp65 specific antibodies. The serum levels of these specific antibodies remained high during 90 days after vaccination, whereas the DNA Me formulation based only in DNA-hsp65 plus TDM-loaded microspheres was not able to sustain the high antibody levels during the same period. Production of IFN- was significant in animals vaccinated with both formulations, while the prime-boost Me vaccinated mice sustained higher levels of this cytokine during all the evaluation period. Thus, prime-boost strategy by using biodegradable microspheres seems to be a promising strategy to stimulate long-lasting immune response.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>15506168</pmid><doi>10.1080/10611860410001723126</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1061-186X |
| ispartof | Journal of drug targeting, 2004-05, Vol.12 (4), p.195-203 |
| issn | 1061-186X 1029-2330 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_1080_10611860410001723126 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Animals Antibodies, Bacterial - blood Bacterial Proteins - administration & dosage Bacterial Proteins - genetics Bacterial Proteins - immunology Chaperonin 60 Chaperonins - administration & dosage Chaperonins - genetics Chaperonins - immunology Cytokines - blood DNA vaccines Hsp65 Immunization, Secondary - methods Interferon-gamma - biosynthesis Lactic Acid - chemistry Lung - pathology Mice Mice, Inbred BALB C Microspheres Mycobacterium tuberculosis Mycobacterium tuberculosis - pathogenicity Particle Size PLGA microspheres Polyglycolic Acid - chemistry Polymers - chemistry Prime boost Recombinant Proteins - administration & dosage Recombinant Proteins - immunology Spleen - metabolism Time Factors Tuberculosis Tuberculosis, Pulmonary - immunology Tuberculosis, Pulmonary - microbiology Tuberculosis, Pulmonary - prevention & control Vaccines, DNA - administration & dosage |
| title | Prime-boost Vaccination based on DNA and Protein-loaded Microspheres for Tuberculosis Prevention |
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