Damage to E. Coli Cells Induced by Tritium Decay: Secondary Lethality under Nongrowth Conditions
Summary Cells containing incorporated 3H-thymidine are damaged by its decay. It was found with E. coli TAU-bar cells that a small part of the damage is lethal whereas most of it is reparable and only potentially lethal. If cells are subjected to nongrowth conditions, the potentially lethal damage ch...
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| Veröffentlicht in: | International journal of radiation biology 1980, Vol.37 (5), p.537-546 |
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| container_end_page | 546 |
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| container_issue | 5 |
| container_start_page | 537 |
| container_title | International journal of radiation biology |
| container_volume | 37 |
| creator | Koukalová, Bla ena Kuhrová, Viera |
| description | Summary
Cells containing incorporated 3H-thymidine are damaged by its decay. It was found with E. coli TAU-bar cells that a small part of the damage is lethal whereas most of it is reparable and only potentially lethal. If cells are subjected to nongrowth conditions, the potentially lethal damage changes to lethal damage. This process is called secondary lethality (SL).
The extent of SL and some changes in DNA under three different modes of growth inhibition were determined. It was found that: (i) SL is maximal under conditions of amino acid starvation (− AA), the viable count decreasing by two orders of magnitude. (ii) SL is 4 times lower in the presence of chloramphenicol (− AA + CLP) and 6·5 times lower under + AA + CLP conditions. Changes in the sedimentation rate of DNA determined in alkaline sucrose gradient correlate with the differences in SL: under − AA conditions the sedimentation rate of DNA decreases whereas in the presence of CLP no decrease occurs. The results suggest that certain enzymatic processes take place under − AA conditions which lead to irreparable changes in DNA. |
| doi_str_mv | 10.1080/09553008014550671 |
| format | Article |
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Cells containing incorporated 3H-thymidine are damaged by its decay. It was found with E. coli TAU-bar cells that a small part of the damage is lethal whereas most of it is reparable and only potentially lethal. If cells are subjected to nongrowth conditions, the potentially lethal damage changes to lethal damage. This process is called secondary lethality (SL).
The extent of SL and some changes in DNA under three different modes of growth inhibition were determined. It was found that: (i) SL is maximal under conditions of amino acid starvation (− AA), the viable count decreasing by two orders of magnitude. (ii) SL is 4 times lower in the presence of chloramphenicol (− AA + CLP) and 6·5 times lower under + AA + CLP conditions. Changes in the sedimentation rate of DNA determined in alkaline sucrose gradient correlate with the differences in SL: under − AA conditions the sedimentation rate of DNA decreases whereas in the presence of CLP no decrease occurs. The results suggest that certain enzymatic processes take place under − AA conditions which lead to irreparable changes in DNA.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0020-7616</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553008014550671</identifier><identifier>PMID: 6995365</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Amino Acids, Essential - pharmacology ; Centrifugation, Density Gradient ; Chloramphenicol - pharmacology ; DNA - analysis ; DNA - radiation effects ; DNA Repair ; Escherichia coli - growth & development ; Escherichia coli - radiation effects ; Mutation ; Thymidine ; Time Factors ; Tritium</subject><ispartof>International journal of radiation biology, 1980, Vol.37 (5), p.537-546</ispartof><rights>1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-24c7e5a9b8a8fd1fc170837b1935244f680e2213065fafcb440a2e527c487b293</citedby><cites>FETCH-LOGICAL-c316t-24c7e5a9b8a8fd1fc170837b1935244f680e2213065fafcb440a2e527c487b293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553008014550671$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553008014550671$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6995365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koukalová, Bla ena</creatorcontrib><creatorcontrib>Kuhrová, Viera</creatorcontrib><title>Damage to E. Coli Cells Induced by Tritium Decay: Secondary Lethality under Nongrowth Conditions</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Summary
Cells containing incorporated 3H-thymidine are damaged by its decay. It was found with E. coli TAU-bar cells that a small part of the damage is lethal whereas most of it is reparable and only potentially lethal. If cells are subjected to nongrowth conditions, the potentially lethal damage changes to lethal damage. This process is called secondary lethality (SL).
The extent of SL and some changes in DNA under three different modes of growth inhibition were determined. It was found that: (i) SL is maximal under conditions of amino acid starvation (− AA), the viable count decreasing by two orders of magnitude. (ii) SL is 4 times lower in the presence of chloramphenicol (− AA + CLP) and 6·5 times lower under + AA + CLP conditions. Changes in the sedimentation rate of DNA determined in alkaline sucrose gradient correlate with the differences in SL: under − AA conditions the sedimentation rate of DNA decreases whereas in the presence of CLP no decrease occurs. The results suggest that certain enzymatic processes take place under − AA conditions which lead to irreparable changes in DNA.</description><subject>Amino Acids, Essential - pharmacology</subject><subject>Centrifugation, Density Gradient</subject><subject>Chloramphenicol - pharmacology</subject><subject>DNA - analysis</subject><subject>DNA - radiation effects</subject><subject>DNA Repair</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - radiation effects</subject><subject>Mutation</subject><subject>Thymidine</subject><subject>Time Factors</subject><subject>Tritium</subject><issn>0955-3002</issn><issn>0020-7616</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kN1KwzAYhoMoc04vwAMhN9CZn6Y_eiTd1MHQA-dxTdMva0ebjLRFevdmbHgieJQXHp6XLy9Ct5TMKUnIPUmF4MQnGgpBopieoSnlEQu4J-doeuA-E3aJrrpuR3wiPJmgSZSmgkdiir4WspVbwL3FyznObFPjDJqmwytTDgpKXIx44-q-Hlq8ACXHB_wByppSuhGvoa9kU_cjHkwJDr9Zs3X2u698kSm9ZE13jS60bDq4Ob0z9Pm83GSvwfr9ZZU9rQPFadQHLFQxCJkWiUx0SbWiMUl4XNCUCxaGOkoIMEY5iYSWWhVhSCQDwWIVJnHBUj5D9NirnO06Bzrfu7r1V-aU5Iex8j9jeefu6OyHooXy1zit4_njkddGW9fKCmTTV0o6yHd2cMb_55_2H6N0deM</recordid><startdate>1980</startdate><enddate>1980</enddate><creator>Koukalová, Bla ena</creator><creator>Kuhrová, Viera</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1980</creationdate><title>Damage to E. Coli Cells Induced by Tritium Decay: Secondary Lethality under Nongrowth Conditions</title><author>Koukalová, Bla ena ; Kuhrová, Viera</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-24c7e5a9b8a8fd1fc170837b1935244f680e2213065fafcb440a2e527c487b293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Amino Acids, Essential - pharmacology</topic><topic>Centrifugation, Density Gradient</topic><topic>Chloramphenicol - pharmacology</topic><topic>DNA - analysis</topic><topic>DNA - radiation effects</topic><topic>DNA Repair</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - radiation effects</topic><topic>Mutation</topic><topic>Thymidine</topic><topic>Time Factors</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koukalová, Bla ena</creatorcontrib><creatorcontrib>Kuhrová, Viera</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koukalová, Bla ena</au><au>Kuhrová, Viera</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Damage to E. Coli Cells Induced by Tritium Decay: Secondary Lethality under Nongrowth Conditions</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1980</date><risdate>1980</risdate><volume>37</volume><issue>5</issue><spage>537</spage><epage>546</epage><pages>537-546</pages><issn>0955-3002</issn><issn>0020-7616</issn><eissn>1362-3095</eissn><abstract>Summary
Cells containing incorporated 3H-thymidine are damaged by its decay. It was found with E. coli TAU-bar cells that a small part of the damage is lethal whereas most of it is reparable and only potentially lethal. If cells are subjected to nongrowth conditions, the potentially lethal damage changes to lethal damage. This process is called secondary lethality (SL).
The extent of SL and some changes in DNA under three different modes of growth inhibition were determined. It was found that: (i) SL is maximal under conditions of amino acid starvation (− AA), the viable count decreasing by two orders of magnitude. (ii) SL is 4 times lower in the presence of chloramphenicol (− AA + CLP) and 6·5 times lower under + AA + CLP conditions. Changes in the sedimentation rate of DNA determined in alkaline sucrose gradient correlate with the differences in SL: under − AA conditions the sedimentation rate of DNA decreases whereas in the presence of CLP no decrease occurs. The results suggest that certain enzymatic processes take place under − AA conditions which lead to irreparable changes in DNA.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>6995365</pmid><doi>10.1080/09553008014550671</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0955-3002 |
| ispartof | International journal of radiation biology, 1980, Vol.37 (5), p.537-546 |
| issn | 0955-3002 0020-7616 1362-3095 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_1080_09553008014550671 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Amino Acids, Essential - pharmacology Centrifugation, Density Gradient Chloramphenicol - pharmacology DNA - analysis DNA - radiation effects DNA Repair Escherichia coli - growth & development Escherichia coli - radiation effects Mutation Thymidine Time Factors Tritium |
| title | Damage to E. Coli Cells Induced by Tritium Decay: Secondary Lethality under Nongrowth Conditions |
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