Radiosensitization of Mammalian Cells by Diamide
Summary The effect of diamide on the radiosensitivity of T-cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0·1, 0·5 and 1·0 mM diamide produced do...
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| Veröffentlicht in: | International journal of radiation biology 1976-01, Vol.29 (6), p.513-522 |
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| container_title | International journal of radiation biology |
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| creator | Vos, O. Grant, G.A. Budke, L. |
| description | Summary
The effect of diamide on the radiosensitivity of T-cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0·1, 0·5 and 1·0 mM diamide produced dose-modifying factors of 0·81, 0·60 and 0·55, respectively. Under anoxic conditions, exposure for 10 min before and during irradiation to 0·5 mM produced a dose-modifying factor of 0·34.
When the cells in oxic conditions were exposed for just 20 min before irradiation, the sensitizing effect was smaller, but some sensitization effect was still apparent after a 120 min interval between diamide treatment and irradiation. Diamide also sensitized the cells after irradiation, but this effect was less than when it was present during irradiation. The presence of whole rat-blood in the incubation medium prevented sensitization. No sensitization could be detected in the whole animal. It is proposed that sensitization is due to lack of capacity for repair of radicals by hydrogen transfer and biochemical repair processes. |
| doi_str_mv | 10.1080/09553007614550611 |
| format | Article |
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The effect of diamide on the radiosensitivity of T-cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0·1, 0·5 and 1·0 mM diamide produced dose-modifying factors of 0·81, 0·60 and 0·55, respectively. Under anoxic conditions, exposure for 10 min before and during irradiation to 0·5 mM produced a dose-modifying factor of 0·34.
When the cells in oxic conditions were exposed for just 20 min before irradiation, the sensitizing effect was smaller, but some sensitization effect was still apparent after a 120 min interval between diamide treatment and irradiation. Diamide also sensitized the cells after irradiation, but this effect was less than when it was present during irradiation. The presence of whole rat-blood in the incubation medium prevented sensitization. No sensitization could be detected in the whole animal. It is proposed that sensitization is due to lack of capacity for repair of radicals by hydrogen transfer and biochemical repair processes.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0020-7616</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553007614550611</identifier><identifier>PMID: 1085760</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Blood ; Cell Line ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Dose-Response Relationship, Drug ; Dose-Response Relationship, Radiation ; Male ; Mice ; Mice, Inbred CBA ; Oxygen ; Radiation-Sensitizing Agents - pharmacology ; Rats ; X-Rays</subject><ispartof>International journal of radiation biology, 1976-01, Vol.29 (6), p.513-522</ispartof><rights>1976 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1976</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-97584af45c06c23092a1a4530c6d88b7c51ef6693a62ea33e1f4eba7bdd80c23</citedby><cites>FETCH-LOGICAL-c401t-97584af45c06c23092a1a4530c6d88b7c51ef6693a62ea33e1f4eba7bdd80c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553007614550611$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553007614550611$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1085760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vos, O.</creatorcontrib><creatorcontrib>Grant, G.A.</creatorcontrib><creatorcontrib>Budke, L.</creatorcontrib><title>Radiosensitization of Mammalian Cells by Diamide</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Summary
The effect of diamide on the radiosensitivity of T-cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0·1, 0·5 and 1·0 mM diamide produced dose-modifying factors of 0·81, 0·60 and 0·55, respectively. Under anoxic conditions, exposure for 10 min before and during irradiation to 0·5 mM produced a dose-modifying factor of 0·34.
When the cells in oxic conditions were exposed for just 20 min before irradiation, the sensitizing effect was smaller, but some sensitization effect was still apparent after a 120 min interval between diamide treatment and irradiation. Diamide also sensitized the cells after irradiation, but this effect was less than when it was present during irradiation. The presence of whole rat-blood in the incubation medium prevented sensitization. No sensitization could be detected in the whole animal. It is proposed that sensitization is due to lack of capacity for repair of radicals by hydrogen transfer and biochemical repair processes.</description><subject>Animals</subject><subject>Blood</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Oxygen</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>Rats</subject><subject>X-Rays</subject><issn>0955-3002</issn><issn>0020-7616</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9j81Kw0AUhQdRaq0-gAshLxC9k_lJgiuJ9QcqgnQfbiYzdEqSKTPpoj69U-NCEFzdAx_f4R5CrincUijgDkohGEAuKRcCJKUnZE6ZzFIWySmZH3nMkJ2TixC2EBOwYkZm0Ra5hDmBD2ytC3oIdrSfOFo3JM4kb9j32Fkckkp3XUiaQ_JosbetviRnBrugr37ugqyfluvqJV29P79WD6tUcaBjWuai4Gi4UCBVFt_JkCKPzyrZFkWTK0G1kbJkKDONjGlquG4wb9q2gCgsCJ1qlXcheG3qnbc9-kNNoT5ur_9sj87N5Oz2Ta_bX8b32MjvJ24H43yPG43duFHodb11ez_EOf-0fwHoKGU3</recordid><startdate>19760101</startdate><enddate>19760101</enddate><creator>Vos, O.</creator><creator>Grant, G.A.</creator><creator>Budke, L.</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19760101</creationdate><title>Radiosensitization of Mammalian Cells by Diamide</title><author>Vos, O. ; Grant, G.A. ; Budke, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-97584af45c06c23092a1a4530c6d88b7c51ef6693a62ea33e1f4eba7bdd80c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Blood</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>Oxygen</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>Rats</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vos, O.</creatorcontrib><creatorcontrib>Grant, G.A.</creatorcontrib><creatorcontrib>Budke, L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vos, O.</au><au>Grant, G.A.</au><au>Budke, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiosensitization of Mammalian Cells by Diamide</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1976-01-01</date><risdate>1976</risdate><volume>29</volume><issue>6</issue><spage>513</spage><epage>522</epage><pages>513-522</pages><issn>0955-3002</issn><issn>0020-7616</issn><eissn>1362-3095</eissn><abstract>Summary
The effect of diamide on the radiosensitivity of T-cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0·1, 0·5 and 1·0 mM diamide produced dose-modifying factors of 0·81, 0·60 and 0·55, respectively. Under anoxic conditions, exposure for 10 min before and during irradiation to 0·5 mM produced a dose-modifying factor of 0·34.
When the cells in oxic conditions were exposed for just 20 min before irradiation, the sensitizing effect was smaller, but some sensitization effect was still apparent after a 120 min interval between diamide treatment and irradiation. Diamide also sensitized the cells after irradiation, but this effect was less than when it was present during irradiation. The presence of whole rat-blood in the incubation medium prevented sensitization. No sensitization could be detected in the whole animal. It is proposed that sensitization is due to lack of capacity for repair of radicals by hydrogen transfer and biochemical repair processes.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>1085760</pmid><doi>10.1080/09553007614550611</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0955-3002 |
| ispartof | International journal of radiation biology, 1976-01, Vol.29 (6), p.513-522 |
| issn | 0955-3002 0020-7616 1362-3095 |
| language | eng |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Animals Blood Cell Line Cell Survival - drug effects Cell Survival - radiation effects Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Male Mice Mice, Inbred CBA Oxygen Radiation-Sensitizing Agents - pharmacology Rats X-Rays |
| title | Radiosensitization of Mammalian Cells by Diamide |
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