The Relationship between Chromosome Aberrations and Low LET Radiation Dose to Human Lymphocytes
Summary In vitro dose-response curves of unstable chromosome aberrations in human lymphocytes have been obtained for 250 kV X-rays and cobalt-60γ-radiation. The aberration yields have been fitted to the quadratic function Y = αD + βD2, which is consistent with the single-track and two-track model fo...
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| Veröffentlicht in: | Int. J. Radiat. Biol., v. 28, no. 1, pp. 75-90 v. 28, no. 1, pp. 75-90, 1975-01, Vol.28 (1), p.75-90 |
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| container_title | Int. J. Radiat. Biol., v. 28, no. 1, pp. 75-90 |
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| creator | Lloyd, D.C. Purrott, R.J. Dolphin, G.W. Bolton, Dawn Edwards, A.A. Corp, M.J. |
| description | Summary
In vitro dose-response curves of unstable chromosome aberrations in human lymphocytes have been obtained for 250 kV X-rays and cobalt-60γ-radiation. The aberration yields have been fitted to the quadratic function Y = αD + βD2, which is consistent with the single-track and two-track model for aberration formation. The values of the coefficients α and β support the hypothesis that the dose-rate effect is limited to the D2 term. The main difference between the coefficients for X- and γ-radiation is in the α values, indicating that X-rays are slightly more efficient, at lower doses, in producing two lesions with a single ionizing track. The lower limits of dose estimate, with 500 cells analysed, are 4 rad for X-rays and 10 rad γ-radiation. Further evidence is presented confirming that, for cytogenetic dosimetry, in vitro dose-response curves should be prepared by irradiating whole blood maintained at 37°C and prior to PHA stimulation. Curves were plotted showing the variation of the number of cells without aberrations with radiation dose and the shape of these curves were compared with those from human cell survival experiments. |
| doi_str_mv | 10.1080/09553007514550781 |
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In vitro dose-response curves of unstable chromosome aberrations in human lymphocytes have been obtained for 250 kV X-rays and cobalt-60γ-radiation. The aberration yields have been fitted to the quadratic function Y = αD + βD2, which is consistent with the single-track and two-track model for aberration formation. The values of the coefficients α and β support the hypothesis that the dose-rate effect is limited to the D2 term. The main difference between the coefficients for X- and γ-radiation is in the α values, indicating that X-rays are slightly more efficient, at lower doses, in producing two lesions with a single ionizing track. The lower limits of dose estimate, with 500 cells analysed, are 4 rad for X-rays and 10 rad γ-radiation. Further evidence is presented confirming that, for cytogenetic dosimetry, in vitro dose-response curves should be prepared by irradiating whole blood maintained at 37°C and prior to PHA stimulation. Curves were plotted showing the variation of the number of cells without aberrations with radiation dose and the shape of these curves were compared with those from human cell survival experiments.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0020-7616</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553007514550781</identifier><identifier>PMID: 1081092</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>560151 --Biomedical Sciences, Applied Studies--Radiation Effects--Radiation Effects on Animals--Man ; Adult ; BIOLOGICAL INDICATORS ; BLOOD ; CHROMOSOMAL ABERRATIONS-- RADIOINDUCTION ; Chromosome Aberrations ; Cobalt Radioisotopes ; COMPARATIVE EVALUATIONS ; CYTOLOGY ; Dose-Response Relationship, Radiation ; DOSE-RESPONSE RELATIONSHIPS-- MATHEMATICAL MODELS ; GAMMA RADIATION ; Gamma Rays ; GENETICS ; Humans ; IN VITRO ; In Vitro Techniques ; IRRADIATION ; Lymphocytes - radiation effects ; LYMPHOCYTES-- BIOLOGICAL RADIATION EFFECTS ; Male ; MAN ; N48510 --Life Sciences--Radiation Effects on Animals--Man ; RADIATION DOSES ; Radiation Genetics ; RADIATION INJURIES ; Radiation Monitoring - methods ; RADIATION QUALITY ; SURVIVAL CURVES ; X RADIATION ; X-Rays</subject><ispartof>Int. J. Radiat. Biol., v. 28, no. 1, pp. 75-90, 1975-01, Vol.28 (1), p.75-90</ispartof><rights>1975 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-e855e50eff1c3c55b16bb22f80d781bc345f8f2574d8bee1303efbc3ada17793</citedby><cites>FETCH-LOGICAL-c428t-e855e50eff1c3c55b16bb22f80d781bc345f8f2574d8bee1303efbc3ada17793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553007514550781$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553007514550781$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,885,27924,27925,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1081092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/4134904$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Lloyd, D.C.</creatorcontrib><creatorcontrib>Purrott, R.J.</creatorcontrib><creatorcontrib>Dolphin, G.W.</creatorcontrib><creatorcontrib>Bolton, Dawn</creatorcontrib><creatorcontrib>Edwards, A.A.</creatorcontrib><creatorcontrib>Corp, M.J.</creatorcontrib><creatorcontrib>National Radiological Protection Board, Harwell, Eng</creatorcontrib><title>The Relationship between Chromosome Aberrations and Low LET Radiation Dose to Human Lymphocytes</title><title>Int. J. Radiat. Biol., v. 28, no. 1, pp. 75-90</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Summary
In vitro dose-response curves of unstable chromosome aberrations in human lymphocytes have been obtained for 250 kV X-rays and cobalt-60γ-radiation. The aberration yields have been fitted to the quadratic function Y = αD + βD2, which is consistent with the single-track and two-track model for aberration formation. The values of the coefficients α and β support the hypothesis that the dose-rate effect is limited to the D2 term. The main difference between the coefficients for X- and γ-radiation is in the α values, indicating that X-rays are slightly more efficient, at lower doses, in producing two lesions with a single ionizing track. The lower limits of dose estimate, with 500 cells analysed, are 4 rad for X-rays and 10 rad γ-radiation. Further evidence is presented confirming that, for cytogenetic dosimetry, in vitro dose-response curves should be prepared by irradiating whole blood maintained at 37°C and prior to PHA stimulation. Curves were plotted showing the variation of the number of cells without aberrations with radiation dose and the shape of these curves were compared with those from human cell survival experiments.</description><subject>560151 --Biomedical Sciences, Applied Studies--Radiation Effects--Radiation Effects on Animals--Man</subject><subject>Adult</subject><subject>BIOLOGICAL INDICATORS</subject><subject>BLOOD</subject><subject>CHROMOSOMAL ABERRATIONS-- RADIOINDUCTION</subject><subject>Chromosome Aberrations</subject><subject>Cobalt Radioisotopes</subject><subject>COMPARATIVE EVALUATIONS</subject><subject>CYTOLOGY</subject><subject>Dose-Response Relationship, Radiation</subject><subject>DOSE-RESPONSE RELATIONSHIPS-- MATHEMATICAL MODELS</subject><subject>GAMMA RADIATION</subject><subject>Gamma Rays</subject><subject>GENETICS</subject><subject>Humans</subject><subject>IN VITRO</subject><subject>In Vitro Techniques</subject><subject>IRRADIATION</subject><subject>Lymphocytes - radiation effects</subject><subject>LYMPHOCYTES-- BIOLOGICAL RADIATION EFFECTS</subject><subject>Male</subject><subject>MAN</subject><subject>N48510 --Life Sciences--Radiation Effects on Animals--Man</subject><subject>RADIATION DOSES</subject><subject>Radiation Genetics</subject><subject>RADIATION INJURIES</subject><subject>Radiation Monitoring - methods</subject><subject>RADIATION QUALITY</subject><subject>SURVIVAL CURVES</subject><subject>X RADIATION</subject><subject>X-Rays</subject><issn>0955-3002</issn><issn>0020-7616</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UF1LwzAUDaLMOf0BPgjB92rSNGuHTzKnEwrC6HtI0hvasTYlyRj792arD4Lg0z3c8wHnIHRPyRMlBXkmC84ZITmnGeckL-gFmlI2TxMWmUs0PfERk_Qa3Xi_JRERVkzQJLopWaRTJKoG8AZ2MrS29007YAXhANDjZeNsZ73tAL8qcG5UYNnXuLQHXK4qvJF1e37jN-sBB4vX-072uDx2Q2P1MYC_RVdG7jzc_dwZqt5X1XKdlF8fn8vXMtFZWoQECs6BEzCGaqY5V3SuVJqagtSxldIs46YwKc-zulAAlBEGJr5lLWmeL9gMPY6x1odWeN0G0I22fQ86iIyybEGyKKKjSDvrvQMjBtd20h0FJeK0p_izZ_Q8jJ5hrzqofznOA0b-ZeTb3ljXyQbkLjRaOhBbu3d97PxP-jcAQIK5</recordid><startdate>19750101</startdate><enddate>19750101</enddate><creator>Lloyd, D.C.</creator><creator>Purrott, R.J.</creator><creator>Dolphin, G.W.</creator><creator>Bolton, Dawn</creator><creator>Edwards, A.A.</creator><creator>Corp, M.J.</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>19750101</creationdate><title>The Relationship between Chromosome Aberrations and Low LET Radiation Dose to Human Lymphocytes</title><author>Lloyd, D.C. ; Purrott, R.J. ; Dolphin, G.W. ; Bolton, Dawn ; Edwards, A.A. ; Corp, M.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-e855e50eff1c3c55b16bb22f80d781bc345f8f2574d8bee1303efbc3ada17793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>560151 --Biomedical Sciences, Applied Studies--Radiation Effects--Radiation Effects on Animals--Man</topic><topic>Adult</topic><topic>BIOLOGICAL INDICATORS</topic><topic>BLOOD</topic><topic>CHROMOSOMAL ABERRATIONS-- RADIOINDUCTION</topic><topic>Chromosome Aberrations</topic><topic>Cobalt Radioisotopes</topic><topic>COMPARATIVE EVALUATIONS</topic><topic>CYTOLOGY</topic><topic>Dose-Response Relationship, Radiation</topic><topic>DOSE-RESPONSE RELATIONSHIPS-- MATHEMATICAL MODELS</topic><topic>GAMMA RADIATION</topic><topic>Gamma Rays</topic><topic>GENETICS</topic><topic>Humans</topic><topic>IN VITRO</topic><topic>In Vitro Techniques</topic><topic>IRRADIATION</topic><topic>Lymphocytes - radiation effects</topic><topic>LYMPHOCYTES-- BIOLOGICAL RADIATION EFFECTS</topic><topic>Male</topic><topic>MAN</topic><topic>N48510 --Life Sciences--Radiation Effects on Animals--Man</topic><topic>RADIATION DOSES</topic><topic>Radiation Genetics</topic><topic>RADIATION INJURIES</topic><topic>Radiation Monitoring - methods</topic><topic>RADIATION QUALITY</topic><topic>SURVIVAL CURVES</topic><topic>X RADIATION</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lloyd, D.C.</creatorcontrib><creatorcontrib>Purrott, R.J.</creatorcontrib><creatorcontrib>Dolphin, G.W.</creatorcontrib><creatorcontrib>Bolton, Dawn</creatorcontrib><creatorcontrib>Edwards, A.A.</creatorcontrib><creatorcontrib>Corp, M.J.</creatorcontrib><creatorcontrib>National Radiological Protection Board, Harwell, Eng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Int. J. Radiat. Biol., v. 28, no. 1, pp. 75-90</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lloyd, D.C.</au><au>Purrott, R.J.</au><au>Dolphin, G.W.</au><au>Bolton, Dawn</au><au>Edwards, A.A.</au><au>Corp, M.J.</au><aucorp>National Radiological Protection Board, Harwell, Eng</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Relationship between Chromosome Aberrations and Low LET Radiation Dose to Human Lymphocytes</atitle><jtitle>Int. J. Radiat. Biol., v. 28, no. 1, pp. 75-90</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1975-01-01</date><risdate>1975</risdate><volume>28</volume><issue>1</issue><spage>75</spage><epage>90</epage><pages>75-90</pages><issn>0955-3002</issn><issn>0020-7616</issn><eissn>1362-3095</eissn><abstract>Summary
In vitro dose-response curves of unstable chromosome aberrations in human lymphocytes have been obtained for 250 kV X-rays and cobalt-60γ-radiation. The aberration yields have been fitted to the quadratic function Y = αD + βD2, which is consistent with the single-track and two-track model for aberration formation. The values of the coefficients α and β support the hypothesis that the dose-rate effect is limited to the D2 term. The main difference between the coefficients for X- and γ-radiation is in the α values, indicating that X-rays are slightly more efficient, at lower doses, in producing two lesions with a single ionizing track. The lower limits of dose estimate, with 500 cells analysed, are 4 rad for X-rays and 10 rad γ-radiation. Further evidence is presented confirming that, for cytogenetic dosimetry, in vitro dose-response curves should be prepared by irradiating whole blood maintained at 37°C and prior to PHA stimulation. Curves were plotted showing the variation of the number of cells without aberrations with radiation dose and the shape of these curves were compared with those from human cell survival experiments.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>1081092</pmid><doi>10.1080/09553007514550781</doi><tpages>16</tpages></addata></record> |
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| identifier | ISSN: 0955-3002 |
| ispartof | Int. J. Radiat. Biol., v. 28, no. 1, pp. 75-90, 1975-01, Vol.28 (1), p.75-90 |
| issn | 0955-3002 0020-7616 1362-3095 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_1080_09553007514550781 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | 560151 --Biomedical Sciences, Applied Studies--Radiation Effects--Radiation Effects on Animals--Man Adult BIOLOGICAL INDICATORS BLOOD CHROMOSOMAL ABERRATIONS-- RADIOINDUCTION Chromosome Aberrations Cobalt Radioisotopes COMPARATIVE EVALUATIONS CYTOLOGY Dose-Response Relationship, Radiation DOSE-RESPONSE RELATIONSHIPS-- MATHEMATICAL MODELS GAMMA RADIATION Gamma Rays GENETICS Humans IN VITRO In Vitro Techniques IRRADIATION Lymphocytes - radiation effects LYMPHOCYTES-- BIOLOGICAL RADIATION EFFECTS Male MAN N48510 --Life Sciences--Radiation Effects on Animals--Man RADIATION DOSES Radiation Genetics RADIATION INJURIES Radiation Monitoring - methods RADIATION QUALITY SURVIVAL CURVES X RADIATION X-Rays |
| title | The Relationship between Chromosome Aberrations and Low LET Radiation Dose to Human Lymphocytes |
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