Repair of Gamma-ray and Neutron-induced Lesions in Germinating Barley Seeds
Summary Barley seeds were irradiated with gamma-rays or fast neutrons in the resting state and after various periods of germination. When irradiated in the resting state, about 50 krads of gamma-rays or about 1 krad of fast neutrons produced a 50 per cent inhibition of seedling growth. The radiosens...
Gespeichert in:
| Veröffentlicht in: | Int. J. Radiat. Biol. 19: No. 5, 433-43(1971) 433-43(1971), 1971, Vol.19 (5), p.433-443 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 443 |
|---|---|
| container_issue | 5 |
| container_start_page | 433 |
| container_title | Int. J. Radiat. Biol. 19: No. 5, 433-43(1971) |
| container_volume | 19 |
| creator | Ahnström, Gunnar Natarajan, A.T. |
| description | Summary
Barley seeds were irradiated with gamma-rays or fast neutrons in the resting state and after various periods of germination. When irradiated in the resting state, about 50 krads of gamma-rays or about 1 krad of fast neutrons produced a 50 per cent inhibition of seedling growth. The radiosensitivity increased after pre-soaking and reached a maximum level after 12-15 hours of germination. At this stage the sensitivity to gamma-rays had increased by a factor of 50 and to fast neutrons by a factor of 5.
The presence of caffeine (assumed to be a DNA-repair inhibitor) during the first period of germination of seeds irradiated in the resting state led to an enhancement of the gamma-induced damage by a factor of 2, but had little effect on neutron-irradiated seeds. The caffeine effect was also negligible if the gamma-irradiated seeds were allowed to germinate for at least 5 hours in water before the treatment. The effect of caffeine was also considerably lower in seeds pre-soaked for 12-15 hours.
The results are explained on the basis of (a) a differential distribution of lesions after gamma-ray and neutron irradiation and (b) a differential organization and mobility of the chromsome material in resting and germinating seeds. |
| doi_str_mv | 10.1080/09553007114550571 |
| format | Article |
| fullrecord | <record><control><sourceid>informahealthcare_osti_</sourceid><recordid>TN_cdi_informahealthcare_journals_10_1080_09553007114550571</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1080_09553007114550571</sourcerecordid><originalsourceid>FETCH-LOGICAL-c343t-73873a85ec1f2be0d6659295948d94e329bc79ca977938bb694de3fe938855073</originalsourceid><addsrcrecordid>eNp9UF1LwzAUDaLMOf0BPgjB92rSJE2DTzp0ikPBj-eSJrcuY01H0j7s3xvp8EXw6Z7L-YBzEDqn5IqSklwTJQQjRFLKhSBC0gM0pazIM5aYQzT94RMm-TE6iXFNEiKsnKCJYJQzzqfo-Q222gXcNXih21ZnQe-w9ha_wNCHzmfO28GAxUuIrvMRO48XEFrnde_8F77TYQM7_A5g4yk6avQmwtn-ztDnw_3H_DFbvi6e5rfLzDDO-kyyUjJdCjC0yWsgtiiEypVQvLSKA8tVbaQyWkmpWFnXheIWWAPpKVNLyWbocsztYu-qaFwPZmU678H0FZd5XpQiiegoMqGLMUBTbYNrddhVlFQ_41V_xkuei9GzHeoW7K9jv1bib0be-aYLrV6B3vQrowNU624IPpX-J_0bExl56w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Repair of Gamma-ray and Neutron-induced Lesions in Germinating Barley Seeds</title><source>Taylor & Francis</source><source>MEDLINE</source><source>Taylor & Francis Medical Library - CRKN</source><creator>Ahnström, Gunnar ; Natarajan, A.T.</creator><creatorcontrib>Ahnström, Gunnar ; Natarajan, A.T. ; Univ. of Stockholm</creatorcontrib><description>Summary
Barley seeds were irradiated with gamma-rays or fast neutrons in the resting state and after various periods of germination. When irradiated in the resting state, about 50 krads of gamma-rays or about 1 krad of fast neutrons produced a 50 per cent inhibition of seedling growth. The radiosensitivity increased after pre-soaking and reached a maximum level after 12-15 hours of germination. At this stage the sensitivity to gamma-rays had increased by a factor of 50 and to fast neutrons by a factor of 5.
The presence of caffeine (assumed to be a DNA-repair inhibitor) during the first period of germination of seeds irradiated in the resting state led to an enhancement of the gamma-induced damage by a factor of 2, but had little effect on neutron-irradiated seeds. The caffeine effect was also negligible if the gamma-irradiated seeds were allowed to germinate for at least 5 hours in water before the treatment. The effect of caffeine was also considerably lower in seeds pre-soaked for 12-15 hours.
The results are explained on the basis of (a) a differential distribution of lesions after gamma-ray and neutron irradiation and (b) a differential organization and mobility of the chromsome material in resting and germinating seeds.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0020-7616</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553007114550571</identifier><identifier>PMID: 5314344</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>ANGIOSPERMIDAE/Hordeum sp., radiation effects on germination of, effects of caffeine on $gamma$ and fast neutron ; BARLEY ; CAFFEINE ; Caffeine - adverse effects ; Chromosome Aberrations ; CHROMOSOMES ; Cobalt Isotopes ; DNA ; DNA - radiation effects ; Edible Grain - radiation effects ; FAST NEUTRONS ; GAMMA RADIATION ; GAMMA RADIATION/effects on germination of barley seed, effects of caffeine on ; GERMINATION ; N28700 --Life Sciences--Radiation Effects on Plants ; Neutrons ; NEUTRONS, FAST/effects on germination of barley seed, effects of caffeine on ; PLANT GROWTH ; Radiation ; RADIATION DOSES ; Radiation Effects ; RADIATION INJURIES ; Radiation-Sensitizing Agents - pharmacology ; REGENERATION ; SEEDLINGS ; Seeds - growth & development ; Seeds - radiation effects ; TIME DEPENDENCE ; Time Factors ; WATER CAFFEINE/effects on germination of fast neutron and gamma-irradiated barley seed</subject><ispartof>Int. J. Radiat. Biol. 19: No. 5, 433-43(1971), 1971, Vol.19 (5), p.433-443</ispartof><rights>1971 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1971</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-73873a85ec1f2be0d6659295948d94e329bc79ca977938bb694de3fe938855073</citedby><cites>FETCH-LOGICAL-c343t-73873a85ec1f2be0d6659295948d94e329bc79ca977938bb694de3fe938855073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553007114550571$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553007114550571$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,881,4010,27900,27901,27902,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5314344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/4722685$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahnström, Gunnar</creatorcontrib><creatorcontrib>Natarajan, A.T.</creatorcontrib><creatorcontrib>Univ. of Stockholm</creatorcontrib><title>Repair of Gamma-ray and Neutron-induced Lesions in Germinating Barley Seeds</title><title>Int. J. Radiat. Biol. 19: No. 5, 433-43(1971)</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Summary
Barley seeds were irradiated with gamma-rays or fast neutrons in the resting state and after various periods of germination. When irradiated in the resting state, about 50 krads of gamma-rays or about 1 krad of fast neutrons produced a 50 per cent inhibition of seedling growth. The radiosensitivity increased after pre-soaking and reached a maximum level after 12-15 hours of germination. At this stage the sensitivity to gamma-rays had increased by a factor of 50 and to fast neutrons by a factor of 5.
The presence of caffeine (assumed to be a DNA-repair inhibitor) during the first period of germination of seeds irradiated in the resting state led to an enhancement of the gamma-induced damage by a factor of 2, but had little effect on neutron-irradiated seeds. The caffeine effect was also negligible if the gamma-irradiated seeds were allowed to germinate for at least 5 hours in water before the treatment. The effect of caffeine was also considerably lower in seeds pre-soaked for 12-15 hours.
The results are explained on the basis of (a) a differential distribution of lesions after gamma-ray and neutron irradiation and (b) a differential organization and mobility of the chromsome material in resting and germinating seeds.</description><subject>ANGIOSPERMIDAE/Hordeum sp., radiation effects on germination of, effects of caffeine on $gamma$ and fast neutron</subject><subject>BARLEY</subject><subject>CAFFEINE</subject><subject>Caffeine - adverse effects</subject><subject>Chromosome Aberrations</subject><subject>CHROMOSOMES</subject><subject>Cobalt Isotopes</subject><subject>DNA</subject><subject>DNA - radiation effects</subject><subject>Edible Grain - radiation effects</subject><subject>FAST NEUTRONS</subject><subject>GAMMA RADIATION</subject><subject>GAMMA RADIATION/effects on germination of barley seed, effects of caffeine on</subject><subject>GERMINATION</subject><subject>N28700 --Life Sciences--Radiation Effects on Plants</subject><subject>Neutrons</subject><subject>NEUTRONS, FAST/effects on germination of barley seed, effects of caffeine on</subject><subject>PLANT GROWTH</subject><subject>Radiation</subject><subject>RADIATION DOSES</subject><subject>Radiation Effects</subject><subject>RADIATION INJURIES</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>REGENERATION</subject><subject>SEEDLINGS</subject><subject>Seeds - growth & development</subject><subject>Seeds - radiation effects</subject><subject>TIME DEPENDENCE</subject><subject>Time Factors</subject><subject>WATER CAFFEINE/effects on germination of fast neutron and gamma-irradiated barley seed</subject><issn>0955-3002</issn><issn>0020-7616</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UF1LwzAUDaLMOf0BPgjB92rSJE2DTzp0ikPBj-eSJrcuY01H0j7s3xvp8EXw6Z7L-YBzEDqn5IqSklwTJQQjRFLKhSBC0gM0pazIM5aYQzT94RMm-TE6iXFNEiKsnKCJYJQzzqfo-Q222gXcNXih21ZnQe-w9ha_wNCHzmfO28GAxUuIrvMRO48XEFrnde_8F77TYQM7_A5g4yk6avQmwtn-ztDnw_3H_DFbvi6e5rfLzDDO-kyyUjJdCjC0yWsgtiiEypVQvLSKA8tVbaQyWkmpWFnXheIWWAPpKVNLyWbocsztYu-qaFwPZmU678H0FZd5XpQiiegoMqGLMUBTbYNrddhVlFQ_41V_xkuei9GzHeoW7K9jv1bib0be-aYLrV6B3vQrowNU624IPpX-J_0bExl56w</recordid><startdate>1971</startdate><enddate>1971</enddate><creator>Ahnström, Gunnar</creator><creator>Natarajan, A.T.</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>1971</creationdate><title>Repair of Gamma-ray and Neutron-induced Lesions in Germinating Barley Seeds</title><author>Ahnström, Gunnar ; Natarajan, A.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-73873a85ec1f2be0d6659295948d94e329bc79ca977938bb694de3fe938855073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>ANGIOSPERMIDAE/Hordeum sp., radiation effects on germination of, effects of caffeine on $gamma$ and fast neutron</topic><topic>BARLEY</topic><topic>CAFFEINE</topic><topic>Caffeine - adverse effects</topic><topic>Chromosome Aberrations</topic><topic>CHROMOSOMES</topic><topic>Cobalt Isotopes</topic><topic>DNA</topic><topic>DNA - radiation effects</topic><topic>Edible Grain - radiation effects</topic><topic>FAST NEUTRONS</topic><topic>GAMMA RADIATION</topic><topic>GAMMA RADIATION/effects on germination of barley seed, effects of caffeine on</topic><topic>GERMINATION</topic><topic>N28700 --Life Sciences--Radiation Effects on Plants</topic><topic>Neutrons</topic><topic>NEUTRONS, FAST/effects on germination of barley seed, effects of caffeine on</topic><topic>PLANT GROWTH</topic><topic>Radiation</topic><topic>RADIATION DOSES</topic><topic>Radiation Effects</topic><topic>RADIATION INJURIES</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>REGENERATION</topic><topic>SEEDLINGS</topic><topic>Seeds - growth & development</topic><topic>Seeds - radiation effects</topic><topic>TIME DEPENDENCE</topic><topic>Time Factors</topic><topic>WATER CAFFEINE/effects on germination of fast neutron and gamma-irradiated barley seed</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahnström, Gunnar</creatorcontrib><creatorcontrib>Natarajan, A.T.</creatorcontrib><creatorcontrib>Univ. of Stockholm</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Int. J. Radiat. Biol. 19: No. 5, 433-43(1971)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahnström, Gunnar</au><au>Natarajan, A.T.</au><aucorp>Univ. of Stockholm</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repair of Gamma-ray and Neutron-induced Lesions in Germinating Barley Seeds</atitle><jtitle>Int. J. Radiat. Biol. 19: No. 5, 433-43(1971)</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1971</date><risdate>1971</risdate><volume>19</volume><issue>5</issue><spage>433</spage><epage>443</epage><pages>433-443</pages><issn>0955-3002</issn><issn>0020-7616</issn><eissn>1362-3095</eissn><abstract>Summary
Barley seeds were irradiated with gamma-rays or fast neutrons in the resting state and after various periods of germination. When irradiated in the resting state, about 50 krads of gamma-rays or about 1 krad of fast neutrons produced a 50 per cent inhibition of seedling growth. The radiosensitivity increased after pre-soaking and reached a maximum level after 12-15 hours of germination. At this stage the sensitivity to gamma-rays had increased by a factor of 50 and to fast neutrons by a factor of 5.
The presence of caffeine (assumed to be a DNA-repair inhibitor) during the first period of germination of seeds irradiated in the resting state led to an enhancement of the gamma-induced damage by a factor of 2, but had little effect on neutron-irradiated seeds. The caffeine effect was also negligible if the gamma-irradiated seeds were allowed to germinate for at least 5 hours in water before the treatment. The effect of caffeine was also considerably lower in seeds pre-soaked for 12-15 hours.
The results are explained on the basis of (a) a differential distribution of lesions after gamma-ray and neutron irradiation and (b) a differential organization and mobility of the chromsome material in resting and germinating seeds.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>5314344</pmid><doi>10.1080/09553007114550571</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0955-3002 |
| ispartof | Int. J. Radiat. Biol. 19: No. 5, 433-43(1971), 1971, Vol.19 (5), p.433-443 |
| issn | 0955-3002 0020-7616 1362-3095 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_1080_09553007114550571 |
| source | Taylor & Francis; MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | ANGIOSPERMIDAE/Hordeum sp., radiation effects on germination of, effects of caffeine on $gamma$ and fast neutron BARLEY CAFFEINE Caffeine - adverse effects Chromosome Aberrations CHROMOSOMES Cobalt Isotopes DNA DNA - radiation effects Edible Grain - radiation effects FAST NEUTRONS GAMMA RADIATION GAMMA RADIATION/effects on germination of barley seed, effects of caffeine on GERMINATION N28700 --Life Sciences--Radiation Effects on Plants Neutrons NEUTRONS, FAST/effects on germination of barley seed, effects of caffeine on PLANT GROWTH Radiation RADIATION DOSES Radiation Effects RADIATION INJURIES Radiation-Sensitizing Agents - pharmacology REGENERATION SEEDLINGS Seeds - growth & development Seeds - radiation effects TIME DEPENDENCE Time Factors WATER CAFFEINE/effects on germination of fast neutron and gamma-irradiated barley seed |
| title | Repair of Gamma-ray and Neutron-induced Lesions in Germinating Barley Seeds |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T14%3A37%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-informahealthcare_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Repair%20of%20Gamma-ray%20and%20Neutron-induced%20Lesions%20in%20Germinating%20Barley%20Seeds&rft.jtitle=Int.%20J.%20Radiat.%20Biol.%2019:%20No.%205,%20433-43(1971)&rft.au=Ahnstr%C3%B6m,%20Gunnar&rft.aucorp=Univ.%20of%20Stockholm&rft.date=1971&rft.volume=19&rft.issue=5&rft.spage=433&rft.epage=443&rft.pages=433-443&rft.issn=0955-3002&rft.eissn=1362-3095&rft_id=info:doi/10.1080/09553007114550571&rft_dat=%3Cinformahealthcare_osti_%3E10_1080_09553007114550571%3C/informahealthcare_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/5314344&rfr_iscdi=true |