The Survival of Micrococcus Radiodurans Irradiated at High LET and the Effect of Acridine Orange
Summary The radiation survival curve of Micrococcus radiodurans consists of a long flat shoulder, followed by an exponential part. When acridine orange is added to the growth medium after irradiation, the shoulder is reduced without changing the final slope. A method is described whereby a measure o...
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| Veröffentlicht in: | Int. J. Radiat. Biol. 16: 583-92(1969) 1969, Vol.16 (6), p.583-592 |
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| container_end_page | 592 |
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| container_issue | 6 |
| container_start_page | 583 |
| container_title | Int. J. Radiat. Biol. 16: 583-92(1969) |
| container_volume | 16 |
| creator | Dewey, D.L. |
| description | Summary
The radiation survival curve of Micrococcus radiodurans consists of a long flat shoulder, followed by an exponential part. When acridine orange is added to the growth medium after irradiation, the shoulder is reduced without changing the final slope. A method is described whereby a measure of repair is obtained from the length of shoulder, and a measure of unrepaired damage from the final slope. With changing LET, these two features of the curve differ. The final slope has a definite optimum LET for maximum sensitivity at a value very near to that for yeast and higher organisms. The length of the shoulder steadily increases as LET increases, suggesting that the target for inactivating the repair mechanism is different from the lethal target. |
| doi_str_mv | 10.1080/09553006914551631 |
| format | Article |
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The radiation survival curve of Micrococcus radiodurans consists of a long flat shoulder, followed by an exponential part. When acridine orange is added to the growth medium after irradiation, the shoulder is reduced without changing the final slope. A method is described whereby a measure of repair is obtained from the length of shoulder, and a measure of unrepaired damage from the final slope. With changing LET, these two features of the curve differ. The final slope has a definite optimum LET for maximum sensitivity at a value very near to that for yeast and higher organisms. The length of the shoulder steadily increases as LET increases, suggesting that the target for inactivating the repair mechanism is different from the lethal target.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0020-7616</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553006914551631</identifier><identifier>PMID: 5308580</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>ACRIDINE ORANGE ; Acridines - pharmacology ; Argon ; Boron ; DYES ; ENERGY LOSSES ; Energy Transfer ; Helium ; LET ; LINEAR ENERGY TRANSFER/effects on survival of irradiated Micrococcus radiodurans ; Lithium ; LOSSES ; Micrococcus - radiation effects ; MICROCOCCUS RADIODURANS ; N28800 --Life Sciences--Radiation Effects on Microorganisms ; Neon ; Oxygen ; Radiation Effects ; RADIATION/effects on survival of Micrococcus radiodurans, effects of acridine orange and LET on ; RADIATIONS ; RADIOSENSITIVITY ; SCHIZOMYCOTA/Micrococcus radiodurans, radiation effects on survival of, effects of acridine orange and LET on ; SURVIVAL TIME ACRIDINE, 3,6-BIS(DIMETHYLAMINO)-/effects on survival of irradiated Micrococcus radiodurans ; Ultraviolet Rays</subject><ispartof>Int. J. Radiat. Biol. 16: 583-92(1969), 1969, Vol.16 (6), p.583-592</ispartof><rights>1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1969</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-b12ac75eec958958f05133632044ab9c50269c27d0954399402d4d152d051253</citedby><cites>FETCH-LOGICAL-c428t-b12ac75eec958958f05133632044ab9c50269c27d0954399402d4d152d051253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553006914551631$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553006914551631$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,881,4010,27900,27901,27902,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5308580$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/4162788$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Dewey, D.L.</creatorcontrib><creatorcontrib>Mount Vernon Hospital, Northwood, Eng</creatorcontrib><title>The Survival of Micrococcus Radiodurans Irradiated at High LET and the Effect of Acridine Orange</title><title>Int. J. Radiat. Biol. 16: 583-92(1969)</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Summary
The radiation survival curve of Micrococcus radiodurans consists of a long flat shoulder, followed by an exponential part. When acridine orange is added to the growth medium after irradiation, the shoulder is reduced without changing the final slope. A method is described whereby a measure of repair is obtained from the length of shoulder, and a measure of unrepaired damage from the final slope. With changing LET, these two features of the curve differ. The final slope has a definite optimum LET for maximum sensitivity at a value very near to that for yeast and higher organisms. The length of the shoulder steadily increases as LET increases, suggesting that the target for inactivating the repair mechanism is different from the lethal target.</description><subject>ACRIDINE ORANGE</subject><subject>Acridines - pharmacology</subject><subject>Argon</subject><subject>Boron</subject><subject>DYES</subject><subject>ENERGY LOSSES</subject><subject>Energy Transfer</subject><subject>Helium</subject><subject>LET</subject><subject>LINEAR ENERGY TRANSFER/effects on survival of irradiated Micrococcus radiodurans</subject><subject>Lithium</subject><subject>LOSSES</subject><subject>Micrococcus - radiation effects</subject><subject>MICROCOCCUS RADIODURANS</subject><subject>N28800 --Life Sciences--Radiation Effects on Microorganisms</subject><subject>Neon</subject><subject>Oxygen</subject><subject>Radiation Effects</subject><subject>RADIATION/effects on survival of Micrococcus radiodurans, effects of acridine orange and LET on</subject><subject>RADIATIONS</subject><subject>RADIOSENSITIVITY</subject><subject>SCHIZOMYCOTA/Micrococcus radiodurans, radiation effects on survival of, effects of acridine orange and LET on</subject><subject>SURVIVAL TIME ACRIDINE, 3,6-BIS(DIMETHYLAMINO)-/effects on survival of irradiated Micrococcus radiodurans</subject><subject>Ultraviolet Rays</subject><issn>0955-3002</issn><issn>0020-7616</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UF1LwzAUDaLMOf0BPgjB92o-uxSfxphuMBlo32uWpGvG1owkHfjvzejwRRAu3BvOBzkHgHuMnjAS6BkVnFOE8gIzznFO8QUYYpqTjCbkEgxPeLoRuQY3IWxRuhAVAzBIKsEFGoKvsjHws_NHe5Q76Gr4bpV3yinVBfghtXW687INcOF9esloNJQRzu2mgctZCWWrYUwWs7o2Kp4MJspbbVsDV0m3Mbfgqpa7YO7OewTK11k5nWfL1dtiOllmihERszUmUo25MargIk2NOKY0pwQxJteF4ojkhSJjnSIxWhQMEc005kQnIuF0BB57WxeirYKy0ahGubZNv6oYzslYiETCPSlFDMGbujp4u5f-u8KoOhVa_Sk0aR56zaFb743-VZwbTPhLj9u2dn4vGyN3sVHSm2rrOt-mzP-4_wCCNH_V</recordid><startdate>1969</startdate><enddate>1969</enddate><creator>Dewey, D.L.</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>1969</creationdate><title>The Survival of Micrococcus Radiodurans Irradiated at High LET and the Effect of Acridine Orange</title><author>Dewey, D.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-b12ac75eec958958f05133632044ab9c50269c27d0954399402d4d152d051253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>ACRIDINE ORANGE</topic><topic>Acridines - pharmacology</topic><topic>Argon</topic><topic>Boron</topic><topic>DYES</topic><topic>ENERGY LOSSES</topic><topic>Energy Transfer</topic><topic>Helium</topic><topic>LET</topic><topic>LINEAR ENERGY TRANSFER/effects on survival of irradiated Micrococcus radiodurans</topic><topic>Lithium</topic><topic>LOSSES</topic><topic>Micrococcus - radiation effects</topic><topic>MICROCOCCUS RADIODURANS</topic><topic>N28800 --Life Sciences--Radiation Effects on Microorganisms</topic><topic>Neon</topic><topic>Oxygen</topic><topic>Radiation Effects</topic><topic>RADIATION/effects on survival of Micrococcus radiodurans, effects of acridine orange and LET on</topic><topic>RADIATIONS</topic><topic>RADIOSENSITIVITY</topic><topic>SCHIZOMYCOTA/Micrococcus radiodurans, radiation effects on survival of, effects of acridine orange and LET on</topic><topic>SURVIVAL TIME ACRIDINE, 3,6-BIS(DIMETHYLAMINO)-/effects on survival of irradiated Micrococcus radiodurans</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dewey, D.L.</creatorcontrib><creatorcontrib>Mount Vernon Hospital, Northwood, Eng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Int. J. Radiat. Biol. 16: 583-92(1969)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dewey, D.L.</au><aucorp>Mount Vernon Hospital, Northwood, Eng</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Survival of Micrococcus Radiodurans Irradiated at High LET and the Effect of Acridine Orange</atitle><jtitle>Int. J. Radiat. Biol. 16: 583-92(1969)</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1969</date><risdate>1969</risdate><volume>16</volume><issue>6</issue><spage>583</spage><epage>592</epage><pages>583-592</pages><issn>0955-3002</issn><issn>0020-7616</issn><eissn>1362-3095</eissn><abstract>Summary
The radiation survival curve of Micrococcus radiodurans consists of a long flat shoulder, followed by an exponential part. When acridine orange is added to the growth medium after irradiation, the shoulder is reduced without changing the final slope. A method is described whereby a measure of repair is obtained from the length of shoulder, and a measure of unrepaired damage from the final slope. With changing LET, these two features of the curve differ. The final slope has a definite optimum LET for maximum sensitivity at a value very near to that for yeast and higher organisms. The length of the shoulder steadily increases as LET increases, suggesting that the target for inactivating the repair mechanism is different from the lethal target.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>5308580</pmid><doi>10.1080/09553006914551631</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0955-3002 |
| ispartof | Int. J. Radiat. Biol. 16: 583-92(1969), 1969, Vol.16 (6), p.583-592 |
| issn | 0955-3002 0020-7616 1362-3095 |
| language | eng |
| recordid | cdi_informahealthcare_journals_10_1080_09553006914551631 |
| source | Taylor & Francis; MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | ACRIDINE ORANGE Acridines - pharmacology Argon Boron DYES ENERGY LOSSES Energy Transfer Helium LET LINEAR ENERGY TRANSFER/effects on survival of irradiated Micrococcus radiodurans Lithium LOSSES Micrococcus - radiation effects MICROCOCCUS RADIODURANS N28800 --Life Sciences--Radiation Effects on Microorganisms Neon Oxygen Radiation Effects RADIATION/effects on survival of Micrococcus radiodurans, effects of acridine orange and LET on RADIATIONS RADIOSENSITIVITY SCHIZOMYCOTA/Micrococcus radiodurans, radiation effects on survival of, effects of acridine orange and LET on SURVIVAL TIME ACRIDINE, 3,6-BIS(DIMETHYLAMINO)-/effects on survival of irradiated Micrococcus radiodurans Ultraviolet Rays |
| title | The Survival of Micrococcus Radiodurans Irradiated at High LET and the Effect of Acridine Orange |
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