Cytokeratin and CDX-2 expression in Barrett's esophagus
Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specime...
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| Veröffentlicht in: | Scandinavian journal of gastroenterology 2008, Vol.43 (2), p.132-140 |
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| creator | van Baal, Jantine W. P. M. Bozikas, Andreas Pronk, Rick Ten Kate, Fibo J. W. Milano, Francesca Rygiel, Agnieszka M. Rosmolen, Wilda D. Peppelenbosch, Maikel P. Bergman, Jacques J. G. H. M. Krishnadath, Kausilia K. |
| description | Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material andmethods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative. |
| doi_str_mv | 10.1080/00365520701676575 |
| format | Article |
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P. M. ; Bozikas, Andreas ; Pronk, Rick ; Ten Kate, Fibo J. W. ; Milano, Francesca ; Rygiel, Agnieszka M. ; Rosmolen, Wilda D. ; Peppelenbosch, Maikel P. ; Bergman, Jacques J. G. H. M. ; Krishnadath, Kausilia K.</creator><creatorcontrib>van Baal, Jantine W. P. M. ; Bozikas, Andreas ; Pronk, Rick ; Ten Kate, Fibo J. W. ; Milano, Francesca ; Rygiel, Agnieszka M. ; Rosmolen, Wilda D. ; Peppelenbosch, Maikel P. ; Bergman, Jacques J. G. H. M. ; Krishnadath, Kausilia K.</creatorcontrib><description>Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material andmethods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.</description><identifier>ISSN: 0036-5521</identifier><identifier>EISSN: 1502-7708</identifier><identifier>DOI: 10.1080/00365520701676575</identifier><identifier>PMID: 18224560</identifier><identifier>CODEN: SJGRA4</identifier><language>eng</language><publisher>Copenhagen: Informa UK Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Barrett Esophagus - metabolism ; Barrett Esophagus - pathology ; Barrett's esophagus ; Biological and medical sciences ; Biomarkers - metabolism ; Biopsy ; Cardia - metabolism ; Cardia - pathology ; CDX-2 ; CDX2 Transcription Factor ; cytokeratin ; Esophagus ; Esophagus - metabolism ; Esophagus - pathology ; Gastroenterology. Liver. Pancreas. Abdomen ; Homeodomain Proteins - metabolism ; Humans ; Keratin-13 - metabolism ; Keratin-18 - metabolism ; Keratin-20 - metabolism ; Keratin-8 - metabolism ; Keratins - metabolism ; Medical sciences ; Middle Aged ; Other diseases. Semiology ; Precancerous Conditions - metabolism ; Precancerous Conditions - pathology</subject><ispartof>Scandinavian journal of gastroenterology, 2008, Vol.43 (2), p.132-140</ispartof><rights>2008 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-81ed3fa5a557b902c68bf35edb93d8b81c8d05fd017c237cdf2082ba89282f823</citedby><cites>FETCH-LOGICAL-c434t-81ed3fa5a557b902c68bf35edb93d8b81c8d05fd017c237cdf2082ba89282f823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00365520701676575$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00365520701676575$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20553683$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18224560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Baal, Jantine W. P. M.</creatorcontrib><creatorcontrib>Bozikas, Andreas</creatorcontrib><creatorcontrib>Pronk, Rick</creatorcontrib><creatorcontrib>Ten Kate, Fibo J. W.</creatorcontrib><creatorcontrib>Milano, Francesca</creatorcontrib><creatorcontrib>Rygiel, Agnieszka M.</creatorcontrib><creatorcontrib>Rosmolen, Wilda D.</creatorcontrib><creatorcontrib>Peppelenbosch, Maikel P.</creatorcontrib><creatorcontrib>Bergman, Jacques J. G. H. M.</creatorcontrib><creatorcontrib>Krishnadath, Kausilia K.</creatorcontrib><title>Cytokeratin and CDX-2 expression in Barrett's esophagus</title><title>Scandinavian journal of gastroenterology</title><addtitle>Scand J Gastroenterol</addtitle><description>Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material andmethods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Barrett Esophagus - metabolism</subject><subject>Barrett Esophagus - pathology</subject><subject>Barrett's esophagus</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Biopsy</subject><subject>Cardia - metabolism</subject><subject>Cardia - pathology</subject><subject>CDX-2</subject><subject>CDX2 Transcription Factor</subject><subject>cytokeratin</subject><subject>Esophagus</subject><subject>Esophagus - metabolism</subject><subject>Esophagus - pathology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Keratin-13 - metabolism</subject><subject>Keratin-18 - metabolism</subject><subject>Keratin-20 - metabolism</subject><subject>Keratin-8 - metabolism</subject><subject>Keratins - metabolism</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Other diseases. Semiology</subject><subject>Precancerous Conditions - metabolism</subject><subject>Precancerous Conditions - pathology</subject><issn>0036-5521</issn><issn>1502-7708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EotuFH8ClygV6ShnbcewILpBCQarEBSRu0cQf3SxJvLUdtfvvyWoDFULqaaSZ5x3NPIS8onBBQcFbAF4KwUACLWUppHhCVlQAy6UE9ZSsDvN8BugJOY1xCwBCFtVzckIVY4UoYUVkvU_-lw2YujHD0WT15c-cZfZ-F2yMnR-zuf8RQ7ApncfMRr_b4M0UX5BnDvtoXy51TX58_vS9_pJff7v6Wn-4znXBi5Qrag13KFAI2VbAdKlax4U1bcWNahXVyoBwBqjUjEttHAPFWlQVU8wpxtfkzXHvLvjbycbUDF3Utu9xtH6KjQRWlADVDNIjqIOPMVjX7EI3YNg3FJqDreY_W3PmbFk-tYM1D4lFzwy8XgCMGnsXcNRd_MsxEIKXis_c-yPXjc6HAe986E2TcN_78CfEH7vj3T_xjcU-bTQG22z9FMZZ8CNf_AYJ9JVR</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>van Baal, Jantine W. P. M.</creator><creator>Bozikas, Andreas</creator><creator>Pronk, Rick</creator><creator>Ten Kate, Fibo J. W.</creator><creator>Milano, Francesca</creator><creator>Rygiel, Agnieszka M.</creator><creator>Rosmolen, Wilda D.</creator><creator>Peppelenbosch, Maikel P.</creator><creator>Bergman, Jacques J. G. H. M.</creator><creator>Krishnadath, Kausilia K.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Scandinavian University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Cytokeratin and CDX-2 expression in Barrett's esophagus</title><author>van Baal, Jantine W. P. M. ; Bozikas, Andreas ; Pronk, Rick ; Ten Kate, Fibo J. W. ; Milano, Francesca ; Rygiel, Agnieszka M. ; Rosmolen, Wilda D. ; Peppelenbosch, Maikel P. ; Bergman, Jacques J. G. H. M. ; Krishnadath, Kausilia K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-81ed3fa5a557b902c68bf35edb93d8b81c8d05fd017c237cdf2082ba89282f823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Barrett Esophagus - metabolism</topic><topic>Barrett Esophagus - pathology</topic><topic>Barrett's esophagus</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Biopsy</topic><topic>Cardia - metabolism</topic><topic>Cardia - pathology</topic><topic>CDX-2</topic><topic>CDX2 Transcription Factor</topic><topic>cytokeratin</topic><topic>Esophagus</topic><topic>Esophagus - metabolism</topic><topic>Esophagus - pathology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Keratin-13 - metabolism</topic><topic>Keratin-18 - metabolism</topic><topic>Keratin-20 - metabolism</topic><topic>Keratin-8 - metabolism</topic><topic>Keratins - metabolism</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Other diseases. Semiology</topic><topic>Precancerous Conditions - metabolism</topic><topic>Precancerous Conditions - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Baal, Jantine W. P. M.</creatorcontrib><creatorcontrib>Bozikas, Andreas</creatorcontrib><creatorcontrib>Pronk, Rick</creatorcontrib><creatorcontrib>Ten Kate, Fibo J. W.</creatorcontrib><creatorcontrib>Milano, Francesca</creatorcontrib><creatorcontrib>Rygiel, Agnieszka M.</creatorcontrib><creatorcontrib>Rosmolen, Wilda D.</creatorcontrib><creatorcontrib>Peppelenbosch, Maikel P.</creatorcontrib><creatorcontrib>Bergman, Jacques J. G. H. M.</creatorcontrib><creatorcontrib>Krishnadath, Kausilia K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of gastroenterology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Baal, Jantine W. P. M.</au><au>Bozikas, Andreas</au><au>Pronk, Rick</au><au>Ten Kate, Fibo J. W.</au><au>Milano, Francesca</au><au>Rygiel, Agnieszka M.</au><au>Rosmolen, Wilda D.</au><au>Peppelenbosch, Maikel P.</au><au>Bergman, Jacques J. G. H. M.</au><au>Krishnadath, Kausilia K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokeratin and CDX-2 expression in Barrett's esophagus</atitle><jtitle>Scandinavian journal of gastroenterology</jtitle><addtitle>Scand J Gastroenterol</addtitle><date>2008</date><risdate>2008</risdate><volume>43</volume><issue>2</issue><spage>132</spage><epage>140</epage><pages>132-140</pages><issn>0036-5521</issn><eissn>1502-7708</eissn><coden>SJGRA4</coden><abstract>Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material andmethods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.</abstract><cop>Copenhagen</cop><cop>Oslo</cop><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>18224560</pmid><doi>10.1080/00365520701676575</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Barrett Esophagus - metabolism Barrett Esophagus - pathology Barrett's esophagus Biological and medical sciences Biomarkers - metabolism Biopsy Cardia - metabolism Cardia - pathology CDX-2 CDX2 Transcription Factor cytokeratin Esophagus Esophagus - metabolism Esophagus - pathology Gastroenterology. Liver. Pancreas. Abdomen Homeodomain Proteins - metabolism Humans Keratin-13 - metabolism Keratin-18 - metabolism Keratin-20 - metabolism Keratin-8 - metabolism Keratins - metabolism Medical sciences Middle Aged Other diseases. Semiology Precancerous Conditions - metabolism Precancerous Conditions - pathology |
| title | Cytokeratin and CDX-2 expression in Barrett's esophagus |
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