Activities of matrix metalloproteinases and tissue inhibitor of metalloproteinase-2 in idiopathic hemotympanum and otitis media with effusion
Conclusion. The expression profile of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-2 (TIMP-2) was specific to the type of middle ear effusion. Further studies are necessary for elucidating its correlation with the sequelae of otitis media with effusion (OME) and idiopat...
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| Veröffentlicht in: | Acta oto-laryngologica 2008-01, Vol.128 (2), p.144-150 |
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| creator | Moon, Sung K. Linthicum Jr, Fred H. Dong Yang, Hae Joo Lee, Seung Park, Keehyun |
| description | Conclusion. The expression profile of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-2 (TIMP-2) was specific to the type of middle ear effusion. Further studies are necessary for elucidating its correlation with the sequelae of otitis media with effusion (OME) and idiopathic hemotympanum. Objectives. We aimed to investigate the relative activities of gelatinases (MMP-2 and -9), stromelysin-1 (MMP-3), and matrilysin-1 (MMP-7), as well as measuring TIMP-2 levels in the serous and mucous effusions of OME and hemorrhagic effusion of the idiopathic hemotympanum. Materials and methods. Middle ear effusions were collected from patients with OME and idiopathic hemotympanum, and were classified as mucoid, serous, or hemorrhagic. MMP activity in the effusion samples was examined by gelatin and casein zymography. Levels of TIMP-2 were measured by ELISA. Human temporal bone sections, with and without otitis media (OM), were examined histologically. Results. One case showed tympanic membrane thinning in the OM group, but none in the control group. While MMP-2 was present in all effusions, the active form of MMP-2 was found only in mucous effusions. MMP-3 and MMP-7 activity was detected only in the mucous effusions. MMP-9 exhibited activity in all effusions, with the highest levels in mucous effusions. TIMP-2 levels were markedly elevated in serous effusions. |
| doi_str_mv | 10.1080/00016480701477610 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_infor</sourceid><recordid>TN_cdi_informahealthcare_journals_10_1080_00016480701477610</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69347454</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-88973693adcf4f013ec5fcea65dd51b7b3cd4ecaba3def977fcbe9c4ca8a421e3</originalsourceid><addsrcrecordid>eNp9kcFu1DAURSMEokPhA9igbGCXYidxHAuEVFVQkCqxgbX14tjkVY4dbKdlPoJ_xtMZCgWpK8t659537VsUzyk5oaQnrwkhtGt7wgltOe8oeVBsaMdoVdeMPiw2u3mVAXFUPInxcncVPXtcHFHeMyqY2BQ_T1XCK0yoY-lNOUMK-KOcdQJr_RJ80ugg5iG4sUwY46pLdBMOmHy4UfyLVnUGShzRL5AmVOWkZ5-28wJunW9sfMr7YlaOCOU1pqnUxqwRvXtaPDJgo352OI-Lrx_efzn7WF18Pv90dnpRKdbUqep7wZtONDAq0xpCG62YURo6No6MDnxo1NhqBQM0ozaCc6MGLVSroIe2pro5Lt7tfZd1yDGUdimAlUvAGcJWekB5d-Jwkt_8laxZ_mfCssGrg0Hw31cdk5wxKm0tOO3XKHO4lreszSDdgyr4GIM2t0sokbsS5X8lZs2Lv9P9URxay8DLAwBRgTUBnMJ4y9XZUAhOM_d2z6EzPsxw7YMdZYKt9eG3qLkvx5s78kmDTZOCoOWlX4PLDd3zil_QM9CS</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69347454</pqid></control><display><type>article</type><title>Activities of matrix metalloproteinases and tissue inhibitor of metalloproteinase-2 in idiopathic hemotympanum and otitis media with effusion</title><source>Taylor & Francis</source><source>MEDLINE</source><source>Taylor & Francis Medical Library - CRKN</source><creator>Moon, Sung K. ; Linthicum Jr, Fred H. ; Dong Yang, Hae ; Joo Lee, Seung ; Park, Keehyun</creator><creatorcontrib>Moon, Sung K. ; Linthicum Jr, Fred H. ; Dong Yang, Hae ; Joo Lee, Seung ; Park, Keehyun</creatorcontrib><description>Conclusion. The expression profile of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-2 (TIMP-2) was specific to the type of middle ear effusion. Further studies are necessary for elucidating its correlation with the sequelae of otitis media with effusion (OME) and idiopathic hemotympanum. Objectives. We aimed to investigate the relative activities of gelatinases (MMP-2 and -9), stromelysin-1 (MMP-3), and matrilysin-1 (MMP-7), as well as measuring TIMP-2 levels in the serous and mucous effusions of OME and hemorrhagic effusion of the idiopathic hemotympanum. Materials and methods. Middle ear effusions were collected from patients with OME and idiopathic hemotympanum, and were classified as mucoid, serous, or hemorrhagic. MMP activity in the effusion samples was examined by gelatin and casein zymography. Levels of TIMP-2 were measured by ELISA. Human temporal bone sections, with and without otitis media (OM), were examined histologically. Results. One case showed tympanic membrane thinning in the OM group, but none in the control group. While MMP-2 was present in all effusions, the active form of MMP-2 was found only in mucous effusions. MMP-3 and MMP-7 activity was detected only in the mucous effusions. MMP-9 exhibited activity in all effusions, with the highest levels in mucous effusions. TIMP-2 levels were markedly elevated in serous effusions.</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.1080/00016480701477610</identifier><identifier>PMID: 17851959</identifier><identifier>CODEN: AOLAAJ</identifier><language>eng</language><publisher>Stockholm: Informa UK Ltd</publisher><subject>Adolescent ; Adult ; Bacterial diseases ; Biological and medical sciences ; Child ; Child, Preschool ; Ear Diseases - pathology ; Ear Diseases - surgery ; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology ; Ear, Middle - pathology ; Ent and stomatologic bacterial diseases ; Enzyme-Linked Immunosorbent Assay ; Female ; Hemorrhage - pathology ; Hemorrhage - surgery ; Human bacterial diseases ; Humans ; idiopathic hemotympanum ; Infectious diseases ; Male ; Matrix metalloproteinase ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 3 - metabolism ; Matrix Metalloproteinase 7 - metabolism ; Matrix Metalloproteinase 9 - metabolism ; Matrix Metalloproteinases - metabolism ; Medical sciences ; Middle Ear Ventilation ; Non tumoral diseases ; otitis media with effusion ; Otitis Media with Effusion - pathology ; Otitis Media with Effusion - surgery ; Otorhinolaryngology. Stomatology ; tissue inhibitor of metalloproteinase ; Tissue Inhibitor of Metalloproteinase-2 - metabolism</subject><ispartof>Acta oto-laryngologica, 2008-01, Vol.128 (2), p.144-150</ispartof><rights>2008 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-88973693adcf4f013ec5fcea65dd51b7b3cd4ecaba3def977fcbe9c4ca8a421e3</citedby><cites>FETCH-LOGICAL-c532t-88973693adcf4f013ec5fcea65dd51b7b3cd4ecaba3def977fcbe9c4ca8a421e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00016480701477610$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00016480701477610$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20169971$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17851959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moon, Sung K.</creatorcontrib><creatorcontrib>Linthicum Jr, Fred H.</creatorcontrib><creatorcontrib>Dong Yang, Hae</creatorcontrib><creatorcontrib>Joo Lee, Seung</creatorcontrib><creatorcontrib>Park, Keehyun</creatorcontrib><title>Activities of matrix metalloproteinases and tissue inhibitor of metalloproteinase-2 in idiopathic hemotympanum and otitis media with effusion</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>Conclusion. The expression profile of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-2 (TIMP-2) was specific to the type of middle ear effusion. Further studies are necessary for elucidating its correlation with the sequelae of otitis media with effusion (OME) and idiopathic hemotympanum. Objectives. We aimed to investigate the relative activities of gelatinases (MMP-2 and -9), stromelysin-1 (MMP-3), and matrilysin-1 (MMP-7), as well as measuring TIMP-2 levels in the serous and mucous effusions of OME and hemorrhagic effusion of the idiopathic hemotympanum. Materials and methods. Middle ear effusions were collected from patients with OME and idiopathic hemotympanum, and were classified as mucoid, serous, or hemorrhagic. MMP activity in the effusion samples was examined by gelatin and casein zymography. Levels of TIMP-2 were measured by ELISA. Human temporal bone sections, with and without otitis media (OM), were examined histologically. Results. One case showed tympanic membrane thinning in the OM group, but none in the control group. While MMP-2 was present in all effusions, the active form of MMP-2 was found only in mucous effusions. MMP-3 and MMP-7 activity was detected only in the mucous effusions. MMP-9 exhibited activity in all effusions, with the highest levels in mucous effusions. TIMP-2 levels were markedly elevated in serous effusions.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Ear Diseases - pathology</subject><subject>Ear Diseases - surgery</subject><subject>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</subject><subject>Ear, Middle - pathology</subject><subject>Ent and stomatologic bacterial diseases</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Hemorrhage - pathology</subject><subject>Hemorrhage - surgery</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>idiopathic hemotympanum</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase 3 - metabolism</subject><subject>Matrix Metalloproteinase 7 - metabolism</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Medical sciences</subject><subject>Middle Ear Ventilation</subject><subject>Non tumoral diseases</subject><subject>otitis media with effusion</subject><subject>Otitis Media with Effusion - pathology</subject><subject>Otitis Media with Effusion - surgery</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>tissue inhibitor of metalloproteinase</subject><subject>Tissue Inhibitor of Metalloproteinase-2 - metabolism</subject><issn>0001-6489</issn><issn>1651-2251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAURSMEokPhA9igbGCXYidxHAuEVFVQkCqxgbX14tjkVY4dbKdlPoJ_xtMZCgWpK8t659537VsUzyk5oaQnrwkhtGt7wgltOe8oeVBsaMdoVdeMPiw2u3mVAXFUPInxcncVPXtcHFHeMyqY2BQ_T1XCK0yoY-lNOUMK-KOcdQJr_RJ80ugg5iG4sUwY46pLdBMOmHy4UfyLVnUGShzRL5AmVOWkZ5-28wJunW9sfMr7YlaOCOU1pqnUxqwRvXtaPDJgo352OI-Lrx_efzn7WF18Pv90dnpRKdbUqep7wZtONDAq0xpCG62YURo6No6MDnxo1NhqBQM0ozaCc6MGLVSroIe2pro5Lt7tfZd1yDGUdimAlUvAGcJWekB5d-Jwkt_8laxZ_mfCssGrg0Hw31cdk5wxKm0tOO3XKHO4lreszSDdgyr4GIM2t0sokbsS5X8lZs2Lv9P9URxay8DLAwBRgTUBnMJ4y9XZUAhOM_d2z6EzPsxw7YMdZYKt9eG3qLkvx5s78kmDTZOCoOWlX4PLDd3zil_QM9CS</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Moon, Sung K.</creator><creator>Linthicum Jr, Fred H.</creator><creator>Dong Yang, Hae</creator><creator>Joo Lee, Seung</creator><creator>Park, Keehyun</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope><scope>5PM</scope></search><sort><creationdate>20080101</creationdate><title>Activities of matrix metalloproteinases and tissue inhibitor of metalloproteinase-2 in idiopathic hemotympanum and otitis media with effusion</title><author>Moon, Sung K. ; Linthicum Jr, Fred H. ; Dong Yang, Hae ; Joo Lee, Seung ; Park, Keehyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-88973693adcf4f013ec5fcea65dd51b7b3cd4ecaba3def977fcbe9c4ca8a421e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Ear Diseases - pathology</topic><topic>Ear Diseases - surgery</topic><topic>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</topic><topic>Ear, Middle - pathology</topic><topic>Ent and stomatologic bacterial diseases</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Hemorrhage - pathology</topic><topic>Hemorrhage - surgery</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>idiopathic hemotympanum</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase 3 - metabolism</topic><topic>Matrix Metalloproteinase 7 - metabolism</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>Matrix Metalloproteinases - metabolism</topic><topic>Medical sciences</topic><topic>Middle Ear Ventilation</topic><topic>Non tumoral diseases</topic><topic>otitis media with effusion</topic><topic>Otitis Media with Effusion - pathology</topic><topic>Otitis Media with Effusion - surgery</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>tissue inhibitor of metalloproteinase</topic><topic>Tissue Inhibitor of Metalloproteinase-2 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moon, Sung K.</creatorcontrib><creatorcontrib>Linthicum Jr, Fred H.</creatorcontrib><creatorcontrib>Dong Yang, Hae</creatorcontrib><creatorcontrib>Joo Lee, Seung</creatorcontrib><creatorcontrib>Park, Keehyun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta oto-laryngologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moon, Sung K.</au><au>Linthicum Jr, Fred H.</au><au>Dong Yang, Hae</au><au>Joo Lee, Seung</au><au>Park, Keehyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activities of matrix metalloproteinases and tissue inhibitor of metalloproteinase-2 in idiopathic hemotympanum and otitis media with effusion</atitle><jtitle>Acta oto-laryngologica</jtitle><addtitle>Acta Otolaryngol</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>128</volume><issue>2</issue><spage>144</spage><epage>150</epage><pages>144-150</pages><issn>0001-6489</issn><eissn>1651-2251</eissn><coden>AOLAAJ</coden><abstract>Conclusion. The expression profile of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-2 (TIMP-2) was specific to the type of middle ear effusion. Further studies are necessary for elucidating its correlation with the sequelae of otitis media with effusion (OME) and idiopathic hemotympanum. Objectives. We aimed to investigate the relative activities of gelatinases (MMP-2 and -9), stromelysin-1 (MMP-3), and matrilysin-1 (MMP-7), as well as measuring TIMP-2 levels in the serous and mucous effusions of OME and hemorrhagic effusion of the idiopathic hemotympanum. Materials and methods. Middle ear effusions were collected from patients with OME and idiopathic hemotympanum, and were classified as mucoid, serous, or hemorrhagic. MMP activity in the effusion samples was examined by gelatin and casein zymography. Levels of TIMP-2 were measured by ELISA. Human temporal bone sections, with and without otitis media (OM), were examined histologically. Results. One case showed tympanic membrane thinning in the OM group, but none in the control group. While MMP-2 was present in all effusions, the active form of MMP-2 was found only in mucous effusions. MMP-3 and MMP-7 activity was detected only in the mucous effusions. MMP-9 exhibited activity in all effusions, with the highest levels in mucous effusions. TIMP-2 levels were markedly elevated in serous effusions.</abstract><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>17851959</pmid><doi>10.1080/00016480701477610</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Bacterial diseases Biological and medical sciences Child Child, Preschool Ear Diseases - pathology Ear Diseases - surgery Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology Ear, Middle - pathology Ent and stomatologic bacterial diseases Enzyme-Linked Immunosorbent Assay Female Hemorrhage - pathology Hemorrhage - surgery Human bacterial diseases Humans idiopathic hemotympanum Infectious diseases Male Matrix metalloproteinase Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 3 - metabolism Matrix Metalloproteinase 7 - metabolism Matrix Metalloproteinase 9 - metabolism Matrix Metalloproteinases - metabolism Medical sciences Middle Ear Ventilation Non tumoral diseases otitis media with effusion Otitis Media with Effusion - pathology Otitis Media with Effusion - surgery Otorhinolaryngology. Stomatology tissue inhibitor of metalloproteinase Tissue Inhibitor of Metalloproteinase-2 - metabolism |
| title | Activities of matrix metalloproteinases and tissue inhibitor of metalloproteinase-2 in idiopathic hemotympanum and otitis media with effusion |
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