(−)-Epigallocatechin-3-gallate protects cultured spiral ganglion cells from H2O2-induced oxidizing damage
Objective Oxidants play an important role in many diseases, including hearing loss. We hypothesized that (−)-epigallocatechin-3-gallate (EGCG) would protect spiral ganglion cells (SGCs) from H2O2-induced oxidizing damage. Material and Methods SGCs of postnatal day 1-3 mice were cultured in vitro. H2...
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| Veröffentlicht in: | Acta oto-laryngologica 2004-05, Vol.124 (4), p.464-470 |
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| container_title | Acta oto-laryngologica |
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| creator | Xie, Dinghua Liu, Guohui Zhu, Ganghua Wu, Weijing Ge, Shenglei |
| description | Objective
Oxidants play an important role in many diseases, including hearing loss. We hypothesized that (−)-epigallocatechin-3-gallate (EGCG) would protect spiral ganglion cells (SGCs) from H2O2-induced oxidizing damage.
Material and Methods
SGCs of postnatal day 1-3 mice were cultured in vitro. H2O2 and EGCG were used at various concentrations. The apoptotic rate of SGCs was evaluated using Hoechst 33 258 staining, and cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Semi-quantitative reverse transcriptase polymerase chain reaction was used to observe manganese superoxide dismutase (MnSOD) gene expression of SGCs treated with H2O2 and EGCG.
Results
The viability of cultured SGCs was significantly decreased, and the apoptotic rate of SGCs significantly increased, at H2O2 concentrations ≥50 μM compared with the control (p |
| doi_str_mv | 10.1080/00016480410018278 |
| format | Article |
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Oxidants play an important role in many diseases, including hearing loss. We hypothesized that (−)-epigallocatechin-3-gallate (EGCG) would protect spiral ganglion cells (SGCs) from H2O2-induced oxidizing damage.
Material and Methods
SGCs of postnatal day 1-3 mice were cultured in vitro. H2O2 and EGCG were used at various concentrations. The apoptotic rate of SGCs was evaluated using Hoechst 33 258 staining, and cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Semi-quantitative reverse transcriptase polymerase chain reaction was used to observe manganese superoxide dismutase (MnSOD) gene expression of SGCs treated with H2O2 and EGCG.
Results
The viability of cultured SGCs was significantly decreased, and the apoptotic rate of SGCs significantly increased, at H2O2 concentrations ≥50 μM compared with the control (p<0.05). MnSOD gene expression was upregulated with increasing H2O2 concentration in cultured SGCs, while this upregulation was suppressed by EGCG.
Conclusion
It is suggested that EGCG, as an antioxidant, significantly protects auditory neurons against H2O2-induced oxidative damage.</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.1080/00016480410018278</identifier><identifier>PMID: 15224876</identifier><identifier>CODEN: AOLAAJ</identifier><language>eng</language><publisher>Stockholm: Informa UK Ltd</publisher><subject>(−)-epigallocatechin-3-gallate ; Animals ; antioxidant enzyme ; Antioxidants - pharmacology ; apoptosis ; Apoptosis - drug effects ; Biological and medical sciences ; Catechin - analogs & derivatives ; Catechin - pharmacology ; Cell Survival - drug effects ; cell viability ; Cells, Cultured ; Gene Expression - drug effects ; H2O2 ; Hydrogen Peroxide - toxicity ; manganese superoxide dismutase gene ; Medical sciences ; Mice ; Otorhinolaryngology. Stomatology ; Oxidation-Reduction ; oxygen free radical ; Reverse Transcriptase Polymerase Chain Reaction ; semi-quantitative reverse transcriptase polymerase chain reaction ; Spiral Ganglion - cytology ; Spiral Ganglion - drug effects ; Spiral Ganglion - metabolism ; spiral ganglion cells ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism ; Up-Regulation</subject><ispartof>Acta oto-laryngologica, 2004-05, Vol.124 (4), p.464-470</ispartof><rights>2004 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2004</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00016480410018278$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00016480410018278$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,23909,23910,25118,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15773712$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15224876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Dinghua</creatorcontrib><creatorcontrib>Liu, Guohui</creatorcontrib><creatorcontrib>Zhu, Ganghua</creatorcontrib><creatorcontrib>Wu, Weijing</creatorcontrib><creatorcontrib>Ge, Shenglei</creatorcontrib><title>(−)-Epigallocatechin-3-gallate protects cultured spiral ganglion cells from H2O2-induced oxidizing damage</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>Objective
Oxidants play an important role in many diseases, including hearing loss. We hypothesized that (−)-epigallocatechin-3-gallate (EGCG) would protect spiral ganglion cells (SGCs) from H2O2-induced oxidizing damage.
Material and Methods
SGCs of postnatal day 1-3 mice were cultured in vitro. H2O2 and EGCG were used at various concentrations. The apoptotic rate of SGCs was evaluated using Hoechst 33 258 staining, and cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Semi-quantitative reverse transcriptase polymerase chain reaction was used to observe manganese superoxide dismutase (MnSOD) gene expression of SGCs treated with H2O2 and EGCG.
Results
The viability of cultured SGCs was significantly decreased, and the apoptotic rate of SGCs significantly increased, at H2O2 concentrations ≥50 μM compared with the control (p<0.05). MnSOD gene expression was upregulated with increasing H2O2 concentration in cultured SGCs, while this upregulation was suppressed by EGCG.
Conclusion
It is suggested that EGCG, as an antioxidant, significantly protects auditory neurons against H2O2-induced oxidative damage.</description><subject>(−)-epigallocatechin-3-gallate</subject><subject>Animals</subject><subject>antioxidant enzyme</subject><subject>Antioxidants - pharmacology</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - pharmacology</subject><subject>Cell Survival - drug effects</subject><subject>cell viability</subject><subject>Cells, Cultured</subject><subject>Gene Expression - drug effects</subject><subject>H2O2</subject><subject>Hydrogen Peroxide - toxicity</subject><subject>manganese superoxide dismutase gene</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Oxidation-Reduction</subject><subject>oxygen free radical</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>semi-quantitative reverse transcriptase polymerase chain reaction</subject><subject>Spiral Ganglion - cytology</subject><subject>Spiral Ganglion - drug effects</subject><subject>Spiral Ganglion - metabolism</subject><subject>spiral ganglion cells</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Up-Regulation</subject><issn>0001-6489</issn><issn>1651-2251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1TAQhS0EopfCA7BB2YDowsV_SRzBBlWlRarUDawtx57kujj2xU4E5QlY9xH7JDjqrQRC1Sw8x_7OaDyD0EtKjimR5B0hhDZCEkFLIlkrH6ENbWqKGavpY7RZ33EBugP0LOerVXayfooOaM2YkG2zQd_e3v6-OcKnOzdq76PRM5itC5jjVRdV7VIsd3OuzOLnJYGt8s4l7atRh9G7GCoD3udqSHGqztklwy7YxRQu_nTW_XJhrKye9AjP0ZNB-wwv9uch-vrp9MvJOb64PPt88vECO8G7GQ-8RPkSlYLVrbBGCqJ7ADFw6E3HmGSDaXrBmewYEEOAgLGdsX3X8B4IP0Rv7uqW1r8vkGc1ubw2qQPEJStGuoY1jBbw1R5c-gms2iU36XSt7sdTgNd7QGej_ZB0MC7_xbUtbykr3Ic7zoUhpkn_iMlbNetrH9O9iVOi1q2p_7ZW7O__sW9B-3lrdAJ1FZcUyrDUw-4_eiybNQ</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Xie, Dinghua</creator><creator>Liu, Guohui</creator><creator>Zhu, Ganghua</creator><creator>Wu, Weijing</creator><creator>Ge, Shenglei</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope></search><sort><creationdate>20040501</creationdate><title>(−)-Epigallocatechin-3-gallate protects cultured spiral ganglion cells from H2O2-induced oxidizing damage</title><author>Xie, Dinghua ; Liu, Guohui ; Zhu, Ganghua ; Wu, Weijing ; Ge, Shenglei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i439t-f3f3f4101842574dc840abee4f3ebc92282fc6b432892e0c0e0ecd9cdb963be03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>(−)-epigallocatechin-3-gallate</topic><topic>Animals</topic><topic>antioxidant enzyme</topic><topic>Antioxidants - pharmacology</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Catechin - analogs & derivatives</topic><topic>Catechin - pharmacology</topic><topic>Cell Survival - drug effects</topic><topic>cell viability</topic><topic>Cells, Cultured</topic><topic>Gene Expression - drug effects</topic><topic>H2O2</topic><topic>Hydrogen Peroxide - toxicity</topic><topic>manganese superoxide dismutase gene</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Oxidation-Reduction</topic><topic>oxygen free radical</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>semi-quantitative reverse transcriptase polymerase chain reaction</topic><topic>Spiral Ganglion - cytology</topic><topic>Spiral Ganglion - drug effects</topic><topic>Spiral Ganglion - metabolism</topic><topic>spiral ganglion cells</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Dinghua</creatorcontrib><creatorcontrib>Liu, Guohui</creatorcontrib><creatorcontrib>Zhu, Ganghua</creatorcontrib><creatorcontrib>Wu, Weijing</creatorcontrib><creatorcontrib>Ge, Shenglei</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><jtitle>Acta oto-laryngologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Dinghua</au><au>Liu, Guohui</au><au>Zhu, Ganghua</au><au>Wu, Weijing</au><au>Ge, Shenglei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>(−)-Epigallocatechin-3-gallate protects cultured spiral ganglion cells from H2O2-induced oxidizing damage</atitle><jtitle>Acta oto-laryngologica</jtitle><addtitle>Acta Otolaryngol</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>124</volume><issue>4</issue><spage>464</spage><epage>470</epage><pages>464-470</pages><issn>0001-6489</issn><eissn>1651-2251</eissn><coden>AOLAAJ</coden><abstract>Objective
Oxidants play an important role in many diseases, including hearing loss. We hypothesized that (−)-epigallocatechin-3-gallate (EGCG) would protect spiral ganglion cells (SGCs) from H2O2-induced oxidizing damage.
Material and Methods
SGCs of postnatal day 1-3 mice were cultured in vitro. H2O2 and EGCG were used at various concentrations. The apoptotic rate of SGCs was evaluated using Hoechst 33 258 staining, and cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Semi-quantitative reverse transcriptase polymerase chain reaction was used to observe manganese superoxide dismutase (MnSOD) gene expression of SGCs treated with H2O2 and EGCG.
Results
The viability of cultured SGCs was significantly decreased, and the apoptotic rate of SGCs significantly increased, at H2O2 concentrations ≥50 μM compared with the control (p<0.05). MnSOD gene expression was upregulated with increasing H2O2 concentration in cultured SGCs, while this upregulation was suppressed by EGCG.
Conclusion
It is suggested that EGCG, as an antioxidant, significantly protects auditory neurons against H2O2-induced oxidative damage.</abstract><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>15224876</pmid><doi>10.1080/00016480410018278</doi><tpages>7</tpages></addata></record> |
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| ispartof | Acta oto-laryngologica, 2004-05, Vol.124 (4), p.464-470 |
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| subjects | (−)-epigallocatechin-3-gallate Animals antioxidant enzyme Antioxidants - pharmacology apoptosis Apoptosis - drug effects Biological and medical sciences Catechin - analogs & derivatives Catechin - pharmacology Cell Survival - drug effects cell viability Cells, Cultured Gene Expression - drug effects H2O2 Hydrogen Peroxide - toxicity manganese superoxide dismutase gene Medical sciences Mice Otorhinolaryngology. Stomatology Oxidation-Reduction oxygen free radical Reverse Transcriptase Polymerase Chain Reaction semi-quantitative reverse transcriptase polymerase chain reaction Spiral Ganglion - cytology Spiral Ganglion - drug effects Spiral Ganglion - metabolism spiral ganglion cells Superoxide Dismutase - genetics Superoxide Dismutase - metabolism Up-Regulation |
| title | (−)-Epigallocatechin-3-gallate protects cultured spiral ganglion cells from H2O2-induced oxidizing damage |
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