Signal enhancement by particle concentration using dielectrophoresis in an electrowetting-driven droplet
This paper describes a new method for enhancing the fluorescence signal of a bead-based immunosensor in a digital microfluidic system by dielectrophoresis (DEP) force. A single droplet containing scattered polystyrene microbeads conjugated with fluorescent dye is first moved by electrowetting to 3-p...
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| creator | Tsung-Min Chuo Po-Huai Shih Wensyang Hsu Cheng-Yeh Huang Yu-Shan Huang Yu-Tong Chen Shih-Kan Fan |
| description | This paper describes a new method for enhancing the fluorescence signal of a bead-based immunosensor in a digital microfluidic system by dielectrophoresis (DEP) force. A single droplet containing scattered polystyrene microbeads conjugated with fluorescent dye is first moved by electrowetting to 3-phase spiral electrodes. The fluorescent microbeads are then concentrated by DEP force at 145 V RMS over 100 kHz. It is shown that the proposed microfluidic chip can evidently enhance the fluorescent intensity even there are only very few fluorescent microbeads (10 to 100), where the droplet volume is only 6.25 nanoliter. The total operation time of the concentrating process is less than 3 minutes. The proposed signal enhancement method holds great potential for bead-based immunoassays to have better limit of detection with short analysis time and small sample volume. |
| doi_str_mv | 10.1109/NANOMED.2012.6509119 |
| format | Conference Proceeding |
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A single droplet containing scattered polystyrene microbeads conjugated with fluorescent dye is first moved by electrowetting to 3-phase spiral electrodes. The fluorescent microbeads are then concentrated by DEP force at 145 V RMS over 100 kHz. It is shown that the proposed microfluidic chip can evidently enhance the fluorescent intensity even there are only very few fluorescent microbeads (10 to 100), where the droplet volume is only 6.25 nanoliter. The total operation time of the concentrating process is less than 3 minutes. The proposed signal enhancement method holds great potential for bead-based immunoassays to have better limit of detection with short analysis time and small sample volume.</description><identifier>ISSN: 2159-6964</identifier><identifier>ISBN: 9781467351010</identifier><identifier>ISBN: 1467351016</identifier><identifier>EISBN: 1467351032</identifier><identifier>EISBN: 9781467351034</identifier><identifier>EISBN: 1467351024</identifier><identifier>EISBN: 9781467351027</identifier><identifier>DOI: 10.1109/NANOMED.2012.6509119</identifier><language>eng</language><publisher>IEEE</publisher><subject>DEP ; fluorescence ; immunoassays ; microbeads ; microfluidics</subject><ispartof>2012 IEEE 6th International Conference on Nano/Molecular Medicine and Engineering (NANOMED), 2012, p.56-60</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/6509119$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>309,310,780,784,789,790,2058,27925,54920</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/6509119$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Tsung-Min Chuo</creatorcontrib><creatorcontrib>Po-Huai Shih</creatorcontrib><creatorcontrib>Wensyang Hsu</creatorcontrib><creatorcontrib>Cheng-Yeh Huang</creatorcontrib><creatorcontrib>Yu-Shan Huang</creatorcontrib><creatorcontrib>Yu-Tong Chen</creatorcontrib><creatorcontrib>Shih-Kan Fan</creatorcontrib><title>Signal enhancement by particle concentration using dielectrophoresis in an electrowetting-driven droplet</title><title>2012 IEEE 6th International Conference on Nano/Molecular Medicine and Engineering (NANOMED)</title><addtitle>NANOMED</addtitle><description>This paper describes a new method for enhancing the fluorescence signal of a bead-based immunosensor in a digital microfluidic system by dielectrophoresis (DEP) force. A single droplet containing scattered polystyrene microbeads conjugated with fluorescent dye is first moved by electrowetting to 3-phase spiral electrodes. The fluorescent microbeads are then concentrated by DEP force at 145 V RMS over 100 kHz. It is shown that the proposed microfluidic chip can evidently enhance the fluorescent intensity even there are only very few fluorescent microbeads (10 to 100), where the droplet volume is only 6.25 nanoliter. The total operation time of the concentrating process is less than 3 minutes. The proposed signal enhancement method holds great potential for bead-based immunoassays to have better limit of detection with short analysis time and small sample volume.</description><subject>DEP</subject><subject>fluorescence</subject><subject>immunoassays</subject><subject>microbeads</subject><subject>microfluidics</subject><issn>2159-6964</issn><isbn>9781467351010</isbn><isbn>1467351016</isbn><isbn>1467351032</isbn><isbn>9781467351034</isbn><isbn>1467351024</isbn><isbn>9781467351027</isbn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2012</creationdate><recordtype>conference_proceeding</recordtype><sourceid>6IE</sourceid><sourceid>RIE</sourceid><recordid>eNo1kM1OAjEUhWvURESeQBd9gcHetjNtlwTxJ0FYqGvS6dxCzdAhnarh7SVxXJ2cL1_O4hByB2wKwMz9arZavy4eppwBn1YlMwDmjFyDrJQogQl-TiZG6f8O7IKMOJSmqEwlr8ik7z8ZYwK05kqNyO4tbKNtKcadjQ73GDOtj_RgUw6uReq6E4052Ry6SL_6ELe0Cdiiy6k77LqEfehpiNRGOtAfzPmkFU0K3xhpc_JazDfk0tu2x8mQY_LxuHifPxfL9dPLfLYsAqgyF1yjdcYwUWvhnJOovPZlhR5kI8vaNAoApHTGO1NDxQ03AphnsnFOeK3FmNz-7QZE3BxS2Nt03AxHiV9Atl2W</recordid><startdate>201211</startdate><enddate>201211</enddate><creator>Tsung-Min Chuo</creator><creator>Po-Huai Shih</creator><creator>Wensyang Hsu</creator><creator>Cheng-Yeh Huang</creator><creator>Yu-Shan Huang</creator><creator>Yu-Tong Chen</creator><creator>Shih-Kan Fan</creator><general>IEEE</general><scope>6IE</scope><scope>6IL</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIL</scope></search><sort><creationdate>201211</creationdate><title>Signal enhancement by particle concentration using dielectrophoresis in an electrowetting-driven droplet</title><author>Tsung-Min Chuo ; Po-Huai Shih ; Wensyang Hsu ; Cheng-Yeh Huang ; Yu-Shan Huang ; Yu-Tong Chen ; Shih-Kan Fan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i175t-28eac9903b83ccc4e7f8f56ef14d45b9d711144c9fc9b162929310f04dcc3f883</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2012</creationdate><topic>DEP</topic><topic>fluorescence</topic><topic>immunoassays</topic><topic>microbeads</topic><topic>microfluidics</topic><toplevel>online_resources</toplevel><creatorcontrib>Tsung-Min Chuo</creatorcontrib><creatorcontrib>Po-Huai Shih</creatorcontrib><creatorcontrib>Wensyang Hsu</creatorcontrib><creatorcontrib>Cheng-Yeh Huang</creatorcontrib><creatorcontrib>Yu-Shan Huang</creatorcontrib><creatorcontrib>Yu-Tong Chen</creatorcontrib><creatorcontrib>Shih-Kan Fan</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan All Online (POP All Online) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Electronic Library (IEL)</collection><collection>IEEE Proceedings Order Plans (POP All) 1998-Present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Tsung-Min Chuo</au><au>Po-Huai Shih</au><au>Wensyang Hsu</au><au>Cheng-Yeh Huang</au><au>Yu-Shan Huang</au><au>Yu-Tong Chen</au><au>Shih-Kan Fan</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Signal enhancement by particle concentration using dielectrophoresis in an electrowetting-driven droplet</atitle><btitle>2012 IEEE 6th International Conference on Nano/Molecular Medicine and Engineering (NANOMED)</btitle><stitle>NANOMED</stitle><date>2012-11</date><risdate>2012</risdate><spage>56</spage><epage>60</epage><pages>56-60</pages><issn>2159-6964</issn><isbn>9781467351010</isbn><isbn>1467351016</isbn><eisbn>1467351032</eisbn><eisbn>9781467351034</eisbn><eisbn>1467351024</eisbn><eisbn>9781467351027</eisbn><abstract>This paper describes a new method for enhancing the fluorescence signal of a bead-based immunosensor in a digital microfluidic system by dielectrophoresis (DEP) force. A single droplet containing scattered polystyrene microbeads conjugated with fluorescent dye is first moved by electrowetting to 3-phase spiral electrodes. The fluorescent microbeads are then concentrated by DEP force at 145 V RMS over 100 kHz. It is shown that the proposed microfluidic chip can evidently enhance the fluorescent intensity even there are only very few fluorescent microbeads (10 to 100), where the droplet volume is only 6.25 nanoliter. The total operation time of the concentrating process is less than 3 minutes. The proposed signal enhancement method holds great potential for bead-based immunoassays to have better limit of detection with short analysis time and small sample volume.</abstract><pub>IEEE</pub><doi>10.1109/NANOMED.2012.6509119</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 2159-6964 |
| ispartof | 2012 IEEE 6th International Conference on Nano/Molecular Medicine and Engineering (NANOMED), 2012, p.56-60 |
| issn | 2159-6964 |
| language | eng |
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| source | IEEE Electronic Library (IEL) Conference Proceedings |
| subjects | DEP fluorescence immunoassays microbeads microfluidics |
| title | Signal enhancement by particle concentration using dielectrophoresis in an electrowetting-driven droplet |
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