Genetic engineering of stem cells by non-viral vectors
Stem/progenitor cells hold a great promise for application in several therapies due to their unique biological characteristics. With the purpose of harnessing these cells full potential in cell-or gene-based therapies it might be advantageous to enhance some of their features through gene delivery s...
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| creator | Madeira, Catarina Ribeiro, Sofia C. Mendes, Rui Pinheiro, Irina S. M. da Silva, Claudia L. Cabral, Joaquim M. S. |
| description | Stem/progenitor cells hold a great promise for application in several therapies due to their unique biological characteristics. With the purpose of harnessing these cells full potential in cell-or gene-based therapies it might be advantageous to enhance some of their features through gene delivery strategies. Accordingly, we are interested in developing efficient and safe methodologies to genetically engineer stem cells, boosting their therapeutic efficacy in Regenerative Medicine. In our work, delivery of plasmid DNA to human Bone Marrow Mesenchymal Stem Cells (BM-MSC) was optimized by lipofection and by a recently available microporation technique and no effect was observed in their immunophenotypic characteristics or differentiative potential. After lipofection similar number of plasmid copies was determined at different cell passages. Importantly, cell proliferation kinetics slowed down due to the presence of plasmid. Overall, we believe our findings are extremely useful towards the maximization of gene delivery to human MSC, without compromising cell function and viability. |
| doi_str_mv | 10.1109/ENBENG.2011.6026044 |
| format | Conference Proceeding |
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M.</creator><creator>da Silva, Claudia L.</creator><creator>Cabral, Joaquim M. S.</creator><general>IEEE</general><scope>6IE</scope><scope>6IL</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIL</scope></search><sort><creationdate>201103</creationdate><title>Genetic engineering of stem cells by non-viral vectors</title><author>Madeira, Catarina ; Ribeiro, Sofia C. ; Mendes, Rui ; Pinheiro, Irina S. M. ; da Silva, Claudia L. ; Cabral, Joaquim M. 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S.</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan All Online (POP All Online) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Electronic Library (IEL)</collection><collection>IEEE Proceedings Order Plans (POP All) 1998-Present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Madeira, Catarina</au><au>Ribeiro, Sofia C.</au><au>Mendes, Rui</au><au>Pinheiro, Irina S. M.</au><au>da Silva, Claudia L.</au><au>Cabral, Joaquim M. 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| fulltext | fulltext_linktorsrc |
| identifier | ISBN: 9781457705229 |
| ispartof | 1st Portuguese Biomedical Engineering Meeting, 2011, p.1-4 |
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| language | eng |
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| source | IEEE Electronic Library (IEL) Conference Proceedings |
| subjects | Biotechnology DNA gene delivery Humans Kinetic theory lipofection microporation plasmid DNA Proteins Stem cells |
| title | Genetic engineering of stem cells by non-viral vectors |
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