Toward detection of harmful algae blooms by in situ surface plasmon resonance spectroscopy
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal se...
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| creator | Laurent, S. Colas, F. Hamelin, M. Crassous, M.-P. Antoine, E. Lehaitre, M. Compere, C. |
| description | Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate. |
| doi_str_mv | 10.1109/SAS.2009.4801771 |
| format | Conference Proceeding |
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Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.</description><identifier>ISBN: 9781424427864</identifier><identifier>ISBN: 142442786X</identifier><identifier>EISBN: 9781424427871</identifier><identifier>EISBN: 1424427878</identifier><identifier>DOI: 10.1109/SAS.2009.4801771</identifier><identifier>LCCN: 2008906677</identifier><language>eng</language><publisher>IEEE</publisher><subject>Algae ; algal detection ; DNA ; Genomics ; Humans ; in situ ; Monitoring ; PCR ; Plasmons ; Resonance ; Sea measurements ; Spectroscopy ; surface plasmon resonance ; Testing</subject><ispartof>2009 IEEE Sensors Applications Symposium, 2009, p.29-33</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/4801771$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>309,310,780,784,789,790,2058,27925,54920</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/4801771$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Laurent, S.</creatorcontrib><creatorcontrib>Colas, F.</creatorcontrib><creatorcontrib>Hamelin, M.</creatorcontrib><creatorcontrib>Crassous, M.-P.</creatorcontrib><creatorcontrib>Antoine, E.</creatorcontrib><creatorcontrib>Lehaitre, M.</creatorcontrib><creatorcontrib>Compere, C.</creatorcontrib><title>Toward detection of harmful algae blooms by in situ surface plasmon resonance spectroscopy</title><title>2009 IEEE Sensors Applications Symposium</title><addtitle>SAS</addtitle><description>Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.</description><subject>Algae</subject><subject>algal detection</subject><subject>DNA</subject><subject>Genomics</subject><subject>Humans</subject><subject>in situ</subject><subject>Monitoring</subject><subject>PCR</subject><subject>Plasmons</subject><subject>Resonance</subject><subject>Sea measurements</subject><subject>Spectroscopy</subject><subject>surface plasmon resonance</subject><subject>Testing</subject><isbn>9781424427864</isbn><isbn>142442786X</isbn><isbn>9781424427871</isbn><isbn>1424427878</isbn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2009</creationdate><recordtype>conference_proceeding</recordtype><sourceid>6IE</sourceid><sourceid>RIE</sourceid><recordid>eNpVUD1rwzAUVCmBtmn2Qhf9gaR6tqyPMYR-QaBD0qVLeJKeWxfbMpJDyb-voVl6y3HH3Q3H2B2IFYCwD7v1blUIYVfSCNAaLtjCagOykLLQRsPlP63kjN1McWOFUlpfsUXO32KCrAprq2v2sY8_mAIPNJIfm9jzWPMvTF19bDm2n0jctTF2mbsTb3qem_HI8zHV6IkPLeZuqiTKscd-cvIwraSYfRxOt2xWY5tpceY5e3963G9eltu359fNertsCtDjMlRYGGmUJVWV3lkg57WVgI58oCC0rAGFgAoMYlBGS1IyBHKm9lILV87Z_d9uQ0SHITUdptPhfE_5C8FIV-w</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Laurent, S.</creator><creator>Colas, F.</creator><creator>Hamelin, M.</creator><creator>Crassous, M.-P.</creator><creator>Antoine, E.</creator><creator>Lehaitre, M.</creator><creator>Compere, C.</creator><general>IEEE</general><scope>6IE</scope><scope>6IL</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIL</scope></search><sort><creationdate>20090101</creationdate><title>Toward detection of harmful algae blooms by in situ surface plasmon resonance spectroscopy</title><author>Laurent, S. ; Colas, F. ; Hamelin, M. ; Crassous, M.-P. ; Antoine, E. ; Lehaitre, M. ; Compere, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i217t-d5a284869e653cb91ebc7941abecded074f1a001518aad6874e64ddeb8fc470b3</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Algae</topic><topic>algal detection</topic><topic>DNA</topic><topic>Genomics</topic><topic>Humans</topic><topic>in situ</topic><topic>Monitoring</topic><topic>PCR</topic><topic>Plasmons</topic><topic>Resonance</topic><topic>Sea measurements</topic><topic>Spectroscopy</topic><topic>surface plasmon resonance</topic><topic>Testing</topic><toplevel>online_resources</toplevel><creatorcontrib>Laurent, S.</creatorcontrib><creatorcontrib>Colas, F.</creatorcontrib><creatorcontrib>Hamelin, M.</creatorcontrib><creatorcontrib>Crassous, M.-P.</creatorcontrib><creatorcontrib>Antoine, E.</creatorcontrib><creatorcontrib>Lehaitre, M.</creatorcontrib><creatorcontrib>Compere, C.</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan All Online (POP All Online) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Electronic Library (IEL)</collection><collection>IEEE Proceedings Order Plans (POP All) 1998-Present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Laurent, S.</au><au>Colas, F.</au><au>Hamelin, M.</au><au>Crassous, M.-P.</au><au>Antoine, E.</au><au>Lehaitre, M.</au><au>Compere, C.</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Toward detection of harmful algae blooms by in situ surface plasmon resonance spectroscopy</atitle><btitle>2009 IEEE Sensors Applications Symposium</btitle><stitle>SAS</stitle><date>2009-01-01</date><risdate>2009</risdate><spage>29</spage><epage>33</epage><pages>29-33</pages><isbn>9781424427864</isbn><isbn>142442786X</isbn><eisbn>9781424427871</eisbn><eisbn>1424427878</eisbn><abstract>Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.</abstract><pub>IEEE</pub><doi>10.1109/SAS.2009.4801771</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | IEEE Electronic Library (IEL) Conference Proceedings |
| subjects | Algae algal detection DNA Genomics Humans in situ Monitoring PCR Plasmons Resonance Sea measurements Spectroscopy surface plasmon resonance Testing |
| title | Toward detection of harmful algae blooms by in situ surface plasmon resonance spectroscopy |
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