An integrated technique for volume estimation of spots in 3-D human cell cultures: Watersnakes

In this paper we propose a new integrated technique to accurately measure the volume of 3-D spots in images of human cell cultures by combining watershed transformation and active contour models. We use the watershed contour as the initial snake contour and develop a novel scheme to estimate the sna...

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Hauptverfasser: Rajagopalan, N., Rodriguez, J.J., Dixon, K.
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Rodriguez, J.J.
Dixon, K.
description In this paper we propose a new integrated technique to accurately measure the volume of 3-D spots in images of human cell cultures by combining watershed transformation and active contour models. We use the watershed contour as the initial snake contour and develop a novel scheme to estimate the snake parameters. Performance of this new technique is measured on synthetic images and images of micro-beads and compared with traditional techniques.
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subjects 3-D active contours
foci detection
Humans
snakes
watershed transformation
title An integrated technique for volume estimation of spots in 3-D human cell cultures: Watersnakes
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