Activation of separated Na+ and K+ channels in an excitable model cell: Using stimulating step pre-pulse waveform
Application of constant step voltage in the presence of channel blocker and different extracellular ionic concentration results in activation or inactivation of one or both K + and Na + channel gates in voltage clamp condition. With recording the membrane currents, the kinetic behavior of ionic chan...
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| creator | Sheikhania, M.F. Ahmadic, M.J. |
| description | Application of constant step voltage in the presence of channel blocker and different extracellular ionic concentration results in activation or inactivation of one or both K + and Na + channel gates in voltage clamp condition. With recording the membrane currents, the kinetic behavior of ionic channel gates are studied. In the present research, the effect of stimulating pre-pulse voltage steps is considered to control the time course of voltage dependent Na + and K + channel activity. Stimulation waveform was designed in order to control the time course of gating and to separate of Na and K channel activity. Then, by applying the stimulus waveform on model and studying the step responses, characteristics and kinetic parameter values were defined. This modeling study has also presented an adequate stimulating signal to separate Na + and K + channel activities with maximum conductance during the stimulation period. |
| doi_str_mv | 10.1109/ISSMDBS.2007.4338306 |
| format | Conference Proceeding |
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With recording the membrane currents, the kinetic behavior of ionic channel gates are studied. In the present research, the effect of stimulating pre-pulse voltage steps is considered to control the time course of voltage dependent Na + and K + channel activity. Stimulation waveform was designed in order to control the time course of gating and to separate of Na and K channel activity. Then, by applying the stimulus waveform on model and studying the step responses, characteristics and kinetic parameter values were defined. This modeling study has also presented an adequate stimulating signal to separate Na + and K + channel activities with maximum conductance during the stimulation period.</description><identifier>ISBN: 9781424413454</identifier><identifier>ISBN: 1424413451</identifier><identifier>EISBN: 9781424413461</identifier><identifier>EISBN: 142441346X</identifier><identifier>DOI: 10.1109/ISSMDBS.2007.4338306</identifier><language>eng</language><publisher>IEEE</publisher><subject>Activation of separated ionic channels ; Biomedical engineering ; Biomembranes ; Biosensors ; Clamps ; Educational institutions ; Kinetic theory ; Physics ; Physiology ; Space vector pulse width modulation ; stimulating waveform ; voltage clamp ; Voltage control</subject><ispartof>2007 4th IEEE/EMBS International Summer School and Symposium on Medical Devices and Biosensors, 2007, p.115-120</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/4338306$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>309,310,776,780,785,786,2051,27904,54899</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/4338306$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Sheikhania, M.F.</creatorcontrib><creatorcontrib>Ahmadic, M.J.</creatorcontrib><title>Activation of separated Na+ and K+ channels in an excitable model cell: Using stimulating step pre-pulse waveform</title><title>2007 4th IEEE/EMBS International Summer School and Symposium on Medical Devices and Biosensors</title><addtitle>ISSMDBS</addtitle><description>Application of constant step voltage in the presence of channel blocker and different extracellular ionic concentration results in activation or inactivation of one or both K + and Na + channel gates in voltage clamp condition. With recording the membrane currents, the kinetic behavior of ionic channel gates are studied. In the present research, the effect of stimulating pre-pulse voltage steps is considered to control the time course of voltage dependent Na + and K + channel activity. Stimulation waveform was designed in order to control the time course of gating and to separate of Na and K channel activity. Then, by applying the stimulus waveform on model and studying the step responses, characteristics and kinetic parameter values were defined. This modeling study has also presented an adequate stimulating signal to separate Na + and K + channel activities with maximum conductance during the stimulation period.</description><subject>Activation of separated ionic channels</subject><subject>Biomedical engineering</subject><subject>Biomembranes</subject><subject>Biosensors</subject><subject>Clamps</subject><subject>Educational institutions</subject><subject>Kinetic theory</subject><subject>Physics</subject><subject>Physiology</subject><subject>Space vector pulse width modulation</subject><subject>stimulating waveform</subject><subject>voltage clamp</subject><subject>Voltage control</subject><isbn>9781424413454</isbn><isbn>1424413451</isbn><isbn>9781424413461</isbn><isbn>142441346X</isbn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2007</creationdate><recordtype>conference_proceeding</recordtype><sourceid>6IE</sourceid><sourceid>RIE</sourceid><recordid>eNpVULtOAzEQNEJIIMgXQLF9dME-P-5MF8IrIkCRUEfGtwYj34PzJcDfY0Eatpmd2Z0phpAzRieMUX0-Xy4fri6Xk5zSYiI4LzlVe2Ski5KJXAjGhWL7_7gUh2QU4ztNw7VMniPyMbWD35rBtw20DiJ2pjcDVvBoxmCaCu7HYN9M02CI4JskAX5ZP5iXgFC3FQawGMIFPEffvEIcfL0JKe53xw66HrNuEyLCp9mia_v6hBw4k4TRDo_J6uZ6NbvLFk-389l0kXlNh6y0haLUGisM10qizqUojOC6yI2sGHcynaoq_WhMRCqU1klHHc8Vr0rFj8npX6xHxHXX-9r03-tdT_wH9N1cmg</recordid><startdate>200708</startdate><enddate>200708</enddate><creator>Sheikhania, M.F.</creator><creator>Ahmadic, M.J.</creator><general>IEEE</general><scope>6IE</scope><scope>6IL</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIL</scope></search><sort><creationdate>200708</creationdate><title>Activation of separated Na+ and K+ channels in an excitable model cell: Using stimulating step pre-pulse waveform</title><author>Sheikhania, M.F. ; Ahmadic, M.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i90t-8c7600cac4a3965e92547a43972a5d13f5ac4dd6009e3f556e5cf5f0f3263d863</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Activation of separated ionic channels</topic><topic>Biomedical engineering</topic><topic>Biomembranes</topic><topic>Biosensors</topic><topic>Clamps</topic><topic>Educational institutions</topic><topic>Kinetic theory</topic><topic>Physics</topic><topic>Physiology</topic><topic>Space vector pulse width modulation</topic><topic>stimulating waveform</topic><topic>voltage clamp</topic><topic>Voltage control</topic><toplevel>online_resources</toplevel><creatorcontrib>Sheikhania, M.F.</creatorcontrib><creatorcontrib>Ahmadic, M.J.</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan All Online (POP All Online) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Electronic Library (IEL)</collection><collection>IEEE Proceedings Order Plans (POP All) 1998-Present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Sheikhania, M.F.</au><au>Ahmadic, M.J.</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Activation of separated Na+ and K+ channels in an excitable model cell: Using stimulating step pre-pulse waveform</atitle><btitle>2007 4th IEEE/EMBS International Summer School and Symposium on Medical Devices and Biosensors</btitle><stitle>ISSMDBS</stitle><date>2007-08</date><risdate>2007</risdate><spage>115</spage><epage>120</epage><pages>115-120</pages><isbn>9781424413454</isbn><isbn>1424413451</isbn><eisbn>9781424413461</eisbn><eisbn>142441346X</eisbn><abstract>Application of constant step voltage in the presence of channel blocker and different extracellular ionic concentration results in activation or inactivation of one or both K + and Na + channel gates in voltage clamp condition. With recording the membrane currents, the kinetic behavior of ionic channel gates are studied. In the present research, the effect of stimulating pre-pulse voltage steps is considered to control the time course of voltage dependent Na + and K + channel activity. Stimulation waveform was designed in order to control the time course of gating and to separate of Na and K channel activity. Then, by applying the stimulus waveform on model and studying the step responses, characteristics and kinetic parameter values were defined. This modeling study has also presented an adequate stimulating signal to separate Na + and K + channel activities with maximum conductance during the stimulation period.</abstract><pub>IEEE</pub><doi>10.1109/ISSMDBS.2007.4338306</doi><tpages>6</tpages></addata></record> |
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| ispartof | 2007 4th IEEE/EMBS International Summer School and Symposium on Medical Devices and Biosensors, 2007, p.115-120 |
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| source | IEEE Electronic Library (IEL) Conference Proceedings |
| subjects | Activation of separated ionic channels Biomedical engineering Biomembranes Biosensors Clamps Educational institutions Kinetic theory Physics Physiology Space vector pulse width modulation stimulating waveform voltage clamp Voltage control |
| title | Activation of separated Na+ and K+ channels in an excitable model cell: Using stimulating step pre-pulse waveform |
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