Integrated sensor arrays for bioluminescence and fluorescence bio-chemical analysis
We present on-chip luminescence and fluorescence bio-chemical analysis, using integrated photodiodes. The detectors and the read-out electronics are implemented on a silicon substrate using standard CMOS processing. The photosensitive structures result from two-stacked PN junctions and an (optional)...
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| creator | Iordanov, V.P. Iliev, B.P. Bossche, A. Bastemeijer, J. Sarro, P.M. Young, I.T. van Dedem, G.W.K. Vellekoop, M.J. |
| description | We present on-chip luminescence and fluorescence bio-chemical analysis, using integrated photodiodes. The detectors and the read-out electronics are implemented on a silicon substrate using standard CMOS processing. The photosensitive structures result from two-stacked PN junctions and an (optional) optical filter. The bioluminescent analyses are based on a light producing reaction - the conversion of ATP (adenosine triphosphate) molecules to AMP - catalyzed by the enzyme luciferase. The obtained results for three different initial concentrations of ATP molecules, in ATP consuming reactions, are presented. Initial fluorescent measurements have been conducted, based on the enzyme protein tyrosine phosphatase (PTP1B) using molecular probes DiFMUP (UV excitable). An enzyme solution (500 pg//spl mu/l) was mixed with DiFMUP. The reaction product DiFMU exhibits excitation/emission maxima of /spl sim/358/455 nm. The undesired excitation (UV) light was filtered out with. an integrated on-chip high pass filter with wavelength cut-off at 400 nm. |
| doi_str_mv | 10.1109/ICSENS.2004.1426293 |
| format | Conference Proceeding |
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The detectors and the read-out electronics are implemented on a silicon substrate using standard CMOS processing. The photosensitive structures result from two-stacked PN junctions and an (optional) optical filter. The bioluminescent analyses are based on a light producing reaction - the conversion of ATP (adenosine triphosphate) molecules to AMP - catalyzed by the enzyme luciferase. The obtained results for three different initial concentrations of ATP molecules, in ATP consuming reactions, are presented. Initial fluorescent measurements have been conducted, based on the enzyme protein tyrosine phosphatase (PTP1B) using molecular probes DiFMUP (UV excitable). An enzyme solution (500 pg//spl mu/l) was mixed with DiFMUP. The reaction product DiFMU exhibits excitation/emission maxima of /spl sim/358/455 nm. The undesired excitation (UV) light was filtered out with. an integrated on-chip high pass filter with wavelength cut-off at 400 nm.</description><identifier>ISBN: 0780386922</identifier><identifier>ISBN: 9780780386921</identifier><identifier>DOI: 10.1109/ICSENS.2004.1426293</identifier><language>eng</language><publisher>Piscataway NJ: IEEE</publisher><subject>Biochemistry ; Bioluminescence ; Biosensors ; CMOS process ; Communication, education, history, and philosophy ; Detectors ; Exact sciences and technology ; Filters, zone plates, and polarizers ; Fluorescence ; Fundamental areas of phenomenology (including applications) ; General equipment and techniques ; Instruments, apparatus, components and techniques common to several branches of physics and astronomy ; Luminescence ; Optical elements, devices, and systems ; Optics ; Photodiodes ; Physics ; Physics literature and publications ; Sensor arrays ; Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing ; Silicon</subject><ispartof>Proceedings of IEEE Sensors, 2004, 2004, p.810-813 vol.2</ispartof><rights>2006 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/1426293$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>309,310,780,784,789,790,2058,4050,4051,27925,54920</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/1426293$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17875520$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Iordanov, V.P.</creatorcontrib><creatorcontrib>Iliev, B.P.</creatorcontrib><creatorcontrib>Bossche, A.</creatorcontrib><creatorcontrib>Bastemeijer, J.</creatorcontrib><creatorcontrib>Sarro, P.M.</creatorcontrib><creatorcontrib>Young, I.T.</creatorcontrib><creatorcontrib>van Dedem, G.W.K.</creatorcontrib><creatorcontrib>Vellekoop, M.J.</creatorcontrib><title>Integrated sensor arrays for bioluminescence and fluorescence bio-chemical analysis</title><title>Proceedings of IEEE Sensors, 2004</title><addtitle>ICSENS</addtitle><description>We present on-chip luminescence and fluorescence bio-chemical analysis, using integrated photodiodes. The detectors and the read-out electronics are implemented on a silicon substrate using standard CMOS processing. The photosensitive structures result from two-stacked PN junctions and an (optional) optical filter. The bioluminescent analyses are based on a light producing reaction - the conversion of ATP (adenosine triphosphate) molecules to AMP - catalyzed by the enzyme luciferase. The obtained results for three different initial concentrations of ATP molecules, in ATP consuming reactions, are presented. Initial fluorescent measurements have been conducted, based on the enzyme protein tyrosine phosphatase (PTP1B) using molecular probes DiFMUP (UV excitable). An enzyme solution (500 pg//spl mu/l) was mixed with DiFMUP. The reaction product DiFMU exhibits excitation/emission maxima of /spl sim/358/455 nm. The undesired excitation (UV) light was filtered out with. an integrated on-chip high pass filter with wavelength cut-off at 400 nm.</description><subject>Biochemistry</subject><subject>Bioluminescence</subject><subject>Biosensors</subject><subject>CMOS process</subject><subject>Communication, education, history, and philosophy</subject><subject>Detectors</subject><subject>Exact sciences and technology</subject><subject>Filters, zone plates, and polarizers</subject><subject>Fluorescence</subject><subject>Fundamental areas of phenomenology (including applications)</subject><subject>General equipment and techniques</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>Luminescence</subject><subject>Optical elements, devices, and systems</subject><subject>Optics</subject><subject>Photodiodes</subject><subject>Physics</subject><subject>Physics literature and publications</subject><subject>Sensor arrays</subject><subject>Sensors (chemical, optical, electrical, movement, gas, etc.); 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Iliev, B.P. ; Bossche, A. ; Bastemeijer, J. ; Sarro, P.M. ; Young, I.T. ; van Dedem, G.W.K. ; Vellekoop, M.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i135t-7aa74f266bc229f5d2780a87e296f995bd2285eb18feb409c3600374a5b5ab4b3</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biochemistry</topic><topic>Bioluminescence</topic><topic>Biosensors</topic><topic>CMOS process</topic><topic>Communication, education, history, and philosophy</topic><topic>Detectors</topic><topic>Exact sciences and technology</topic><topic>Filters, zone plates, and polarizers</topic><topic>Fluorescence</topic><topic>Fundamental areas of phenomenology (including applications)</topic><topic>General equipment and techniques</topic><topic>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</topic><topic>Luminescence</topic><topic>Optical elements, devices, and systems</topic><topic>Optics</topic><topic>Photodiodes</topic><topic>Physics</topic><topic>Physics literature and publications</topic><topic>Sensor arrays</topic><topic>Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing</topic><topic>Silicon</topic><toplevel>online_resources</toplevel><creatorcontrib>Iordanov, V.P.</creatorcontrib><creatorcontrib>Iliev, B.P.</creatorcontrib><creatorcontrib>Bossche, A.</creatorcontrib><creatorcontrib>Bastemeijer, J.</creatorcontrib><creatorcontrib>Sarro, P.M.</creatorcontrib><creatorcontrib>Young, I.T.</creatorcontrib><creatorcontrib>van Dedem, G.W.K.</creatorcontrib><creatorcontrib>Vellekoop, M.J.</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan (POP) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Electronic Library (IEL)</collection><collection>IEEE Proceedings Order Plans (POP) 1998-present</collection><collection>Pascal-Francis</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Iordanov, V.P.</au><au>Iliev, B.P.</au><au>Bossche, A.</au><au>Bastemeijer, J.</au><au>Sarro, P.M.</au><au>Young, I.T.</au><au>van Dedem, G.W.K.</au><au>Vellekoop, M.J.</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Integrated sensor arrays for bioluminescence and fluorescence bio-chemical analysis</atitle><btitle>Proceedings of IEEE Sensors, 2004</btitle><stitle>ICSENS</stitle><date>2004</date><risdate>2004</risdate><spage>810</spage><epage>813 vol.2</epage><pages>810-813 vol.2</pages><isbn>0780386922</isbn><isbn>9780780386921</isbn><abstract>We present on-chip luminescence and fluorescence bio-chemical analysis, using integrated photodiodes. The detectors and the read-out electronics are implemented on a silicon substrate using standard CMOS processing. The photosensitive structures result from two-stacked PN junctions and an (optional) optical filter. The bioluminescent analyses are based on a light producing reaction - the conversion of ATP (adenosine triphosphate) molecules to AMP - catalyzed by the enzyme luciferase. The obtained results for three different initial concentrations of ATP molecules, in ATP consuming reactions, are presented. Initial fluorescent measurements have been conducted, based on the enzyme protein tyrosine phosphatase (PTP1B) using molecular probes DiFMUP (UV excitable). An enzyme solution (500 pg//spl mu/l) was mixed with DiFMUP. The reaction product DiFMU exhibits excitation/emission maxima of /spl sim/358/455 nm. The undesired excitation (UV) light was filtered out with. an integrated on-chip high pass filter with wavelength cut-off at 400 nm.</abstract><cop>Piscataway NJ</cop><pub>IEEE</pub><doi>10.1109/ICSENS.2004.1426293</doi></addata></record> |
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| subjects | Biochemistry Bioluminescence Biosensors CMOS process Communication, education, history, and philosophy Detectors Exact sciences and technology Filters, zone plates, and polarizers Fluorescence Fundamental areas of phenomenology (including applications) General equipment and techniques Instruments, apparatus, components and techniques common to several branches of physics and astronomy Luminescence Optical elements, devices, and systems Optics Photodiodes Physics Physics literature and publications Sensor arrays Sensors (chemical, optical, electrical, movement, gas, etc.) remote sensing Silicon |
| title | Integrated sensor arrays for bioluminescence and fluorescence bio-chemical analysis |
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