Mathematical analysis of alveolar macrophage pH regulation
Alveolar macrophage cell pH is maintained by the complex interactions of three acid-base transporters: plasmalemma vacuolar-type H/sup +/-ATPase (V-ATPase), Na/sup +//H/sup +/ exchanger, and Na/sup +/-independent HCO/sub 3//sup -/ /Cl/sup /exchanger. Since V-ATPase-mediated H/sup +/ extrusion is ele...
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| creator | Luo, C. Clark, J.W. Heming, T.A. Bidani, A. |
| description | Alveolar macrophage cell pH is maintained by the complex interactions of three acid-base transporters: plasmalemma vacuolar-type H/sup +/-ATPase (V-ATPase), Na/sup +//H/sup +/ exchanger, and Na/sup +/-independent HCO/sub 3//sup -/ /Cl/sup /exchanger. Since V-ATPase-mediated H/sup +/ extrusion is electrogenic, alterations in membrane potential (and cell volume) occur during experimental acid-loading and subsequent pH/sub i/ recovery. We have developed a nonlinear mathematical model that incorporates the biophysical determinants of cell pH, cell volume, the transmembrane fluxes of individual acid-base equivalents, fluxes of Na/sup +/, K/sup +/, and Cl/sup -/, and associated ion currents through ion channels. The model was used to analyze the acid-base responses of rabbit alveolar m/spl phi/ to an NH/sub 3/ prepulse acid-load. Our results indicate that there are substantial problems with quantifying transporter-mediated H/sup +/ efflux solely from experimental observations of pH/sub i/ recovery due to residual NH/sub 4//sup +/ efflux from the macrophage. |
| doi_str_mv | 10.1109/IEMBS.2002.1106526 |
| format | Conference Proceeding |
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Since V-ATPase-mediated H/sup +/ extrusion is electrogenic, alterations in membrane potential (and cell volume) occur during experimental acid-loading and subsequent pH/sub i/ recovery. We have developed a nonlinear mathematical model that incorporates the biophysical determinants of cell pH, cell volume, the transmembrane fluxes of individual acid-base equivalents, fluxes of Na/sup +/, K/sup +/, and Cl/sup -/, and associated ion currents through ion channels. The model was used to analyze the acid-base responses of rabbit alveolar m/spl phi/ to an NH/sub 3/ prepulse acid-load. Our results indicate that there are substantial problems with quantifying transporter-mediated H/sup +/ efflux solely from experimental observations of pH/sub i/ recovery due to residual NH/sub 4//sup +/ efflux from the macrophage.</description><identifier>ISSN: 1094-687X</identifier><identifier>ISBN: 0780376129</identifier><identifier>ISBN: 9780780376120</identifier><identifier>EISSN: 1558-4615</identifier><identifier>DOI: 10.1109/IEMBS.2002.1106526</identifier><language>eng</language><publisher>IEEE</publisher><subject>Biomembranes ; Cells (biology) ; Coupled mode analysis ; Mathematical analysis ; Mathematical model ; Nuclear and plasma sciences ; Plasma measurements ; Plasma transport processes ; Production ; Rabbits</subject><ispartof>Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology, 2002, Vol.2, p.1540-1541 vol.2</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/1106526$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>309,310,780,784,789,790,2058,4050,4051,27925,54920</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/1106526$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Luo, C.</creatorcontrib><creatorcontrib>Clark, J.W.</creatorcontrib><creatorcontrib>Heming, T.A.</creatorcontrib><creatorcontrib>Bidani, A.</creatorcontrib><title>Mathematical analysis of alveolar macrophage pH regulation</title><title>Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology</title><addtitle>IEMBS</addtitle><description>Alveolar macrophage cell pH is maintained by the complex interactions of three acid-base transporters: plasmalemma vacuolar-type H/sup +/-ATPase (V-ATPase), Na/sup +//H/sup +/ exchanger, and Na/sup +/-independent HCO/sub 3//sup -/ /Cl/sup /exchanger. Since V-ATPase-mediated H/sup +/ extrusion is electrogenic, alterations in membrane potential (and cell volume) occur during experimental acid-loading and subsequent pH/sub i/ recovery. We have developed a nonlinear mathematical model that incorporates the biophysical determinants of cell pH, cell volume, the transmembrane fluxes of individual acid-base equivalents, fluxes of Na/sup +/, K/sup +/, and Cl/sup -/, and associated ion currents through ion channels. The model was used to analyze the acid-base responses of rabbit alveolar m/spl phi/ to an NH/sub 3/ prepulse acid-load. Our results indicate that there are substantial problems with quantifying transporter-mediated H/sup +/ efflux solely from experimental observations of pH/sub i/ recovery due to residual NH/sub 4//sup +/ efflux from the macrophage.</description><subject>Biomembranes</subject><subject>Cells (biology)</subject><subject>Coupled mode analysis</subject><subject>Mathematical analysis</subject><subject>Mathematical model</subject><subject>Nuclear and plasma sciences</subject><subject>Plasma measurements</subject><subject>Plasma transport processes</subject><subject>Production</subject><subject>Rabbits</subject><issn>1094-687X</issn><issn>1558-4615</issn><isbn>0780376129</isbn><isbn>9780780376120</isbn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2002</creationdate><recordtype>conference_proceeding</recordtype><sourceid>6IE</sourceid><sourceid>RIE</sourceid><recordid>eNp9jrsOgjAYhRsviai8gC59AbCFtoCjRoMDkw5u5A8pF1MuadGEtxcTZs_y5eQ7w0FoR4lLKYkOt0tyurseId6vC-6JGbIo56HDBOVztCZBSPxAUC9ajIJEzBFh8Fwh25gXGcM4ZSyy0DGBvpQ19FUGCkMDajCVwW2OQX1kq0DjGjLddiUUEncx1rJ4q3HeNlu0zEEZaU_coP318jjHTiWlTDtd1aCHdLrn_7dfvoA67Q</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Luo, C.</creator><creator>Clark, J.W.</creator><creator>Heming, T.A.</creator><creator>Bidani, A.</creator><general>IEEE</general><scope>6IE</scope><scope>6IH</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIO</scope></search><sort><creationdate>2002</creationdate><title>Mathematical analysis of alveolar macrophage pH regulation</title><author>Luo, C. ; Clark, J.W. ; Heming, T.A. ; Bidani, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-ieee_primary_11065263</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biomembranes</topic><topic>Cells (biology)</topic><topic>Coupled mode analysis</topic><topic>Mathematical analysis</topic><topic>Mathematical model</topic><topic>Nuclear and plasma sciences</topic><topic>Plasma measurements</topic><topic>Plasma transport processes</topic><topic>Production</topic><topic>Rabbits</topic><toplevel>online_resources</toplevel><creatorcontrib>Luo, C.</creatorcontrib><creatorcontrib>Clark, J.W.</creatorcontrib><creatorcontrib>Heming, T.A.</creatorcontrib><creatorcontrib>Bidani, A.</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan (POP) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Electronic Library (IEL)</collection><collection>IEEE Proceedings Order Plans (POP) 1998-present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Luo, C.</au><au>Clark, J.W.</au><au>Heming, T.A.</au><au>Bidani, A.</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Mathematical analysis of alveolar macrophage pH regulation</atitle><btitle>Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology</btitle><stitle>IEMBS</stitle><date>2002</date><risdate>2002</risdate><volume>2</volume><spage>1540</spage><epage>1541 vol.2</epage><pages>1540-1541 vol.2</pages><issn>1094-687X</issn><eissn>1558-4615</eissn><isbn>0780376129</isbn><isbn>9780780376120</isbn><abstract>Alveolar macrophage cell pH is maintained by the complex interactions of three acid-base transporters: plasmalemma vacuolar-type H/sup +/-ATPase (V-ATPase), Na/sup +//H/sup +/ exchanger, and Na/sup +/-independent HCO/sub 3//sup -/ /Cl/sup /exchanger. Since V-ATPase-mediated H/sup +/ extrusion is electrogenic, alterations in membrane potential (and cell volume) occur during experimental acid-loading and subsequent pH/sub i/ recovery. We have developed a nonlinear mathematical model that incorporates the biophysical determinants of cell pH, cell volume, the transmembrane fluxes of individual acid-base equivalents, fluxes of Na/sup +/, K/sup +/, and Cl/sup -/, and associated ion currents through ion channels. The model was used to analyze the acid-base responses of rabbit alveolar m/spl phi/ to an NH/sub 3/ prepulse acid-load. Our results indicate that there are substantial problems with quantifying transporter-mediated H/sup +/ efflux solely from experimental observations of pH/sub i/ recovery due to residual NH/sub 4//sup +/ efflux from the macrophage.</abstract><pub>IEEE</pub><doi>10.1109/IEMBS.2002.1106526</doi></addata></record> |
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| ispartof | Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology, 2002, Vol.2, p.1540-1541 vol.2 |
| issn | 1094-687X 1558-4615 |
| language | eng |
| recordid | cdi_ieee_primary_1106526 |
| source | IEEE Electronic Library (IEL) Conference Proceedings |
| subjects | Biomembranes Cells (biology) Coupled mode analysis Mathematical analysis Mathematical model Nuclear and plasma sciences Plasma measurements Plasma transport processes Production Rabbits |
| title | Mathematical analysis of alveolar macrophage pH regulation |
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