HPLC Method Development and Validation for Quantification of Berberine from Berberis aristata and Berberis tinctoria
An RP-HPLC method with photodiode array detection has been developed for the determination of major constituent berberine from Berberis aristata (Barberry) and Berberis tinctoria. Berberine was isolated from the plant extract on semi-preparative HPLC and separated on HPLC by using an isocratic mode...
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| Veröffentlicht in: | International Journal of Applied Science and Engineering 2013-06, Vol.11 (2), p.203-211 |
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| container_title | International Journal of Applied Science and Engineering |
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| creator | Hemant Shigwan Arvind Saklani P. D. Hamrapurkar Tukaram Manea Priyanka Bhattb |
| description | An RP-HPLC method with photodiode array detection has been developed for the determination of major constituent berberine from Berberis aristata (Barberry) and Berberis tinctoria. Berberine was isolated from the plant extract on semi-preparative HPLC and separated on HPLC by using an isocratic mode consisting of 0.1% trifloroacetic acid: acetonitrile (60:40， v/v) at a flow rate of 1 mL/min. Under these conditions’ a plot of integrated peak area versus concentration of berberine was found be linear over the concentration range of 0.2 |ig/mL to 150 |ig/mL. The limit of detection was 1ng on column and limit of quantification was 2 ng on column for berberine. The berberine content in B. aristata and B. tinctoria was found to be 3.18% and 1.46% respectively. |
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Hamrapurkar ; Tukaram Manea ; Priyanka Bhattb</creatorcontrib><description>An RP-HPLC method with photodiode array detection has been developed for the determination of major constituent berberine from Berberis aristata (Barberry) and Berberis tinctoria. Berberine was isolated from the plant extract on semi-preparative HPLC and separated on HPLC by using an isocratic mode consisting of 0.1% trifloroacetic acid: acetonitrile (60:40， v/v) at a flow rate of 1 mL/min. Under these conditions’ a plot of integrated peak area versus concentration of berberine was found be linear over the concentration range of 0.2 |ig/mL to 150 |ig/mL. The limit of detection was 1ng on column and limit of quantification was 2 ng on column for berberine. 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The limit of detection was 1ng on column and limit of quantification was 2 ng on column for berberine. The berberine content in B. aristata and B. tinctoria was found to be 3.18% and 1.46% respectively.</description><subject>Berberine</subject><subject>Photo Diode Array detector</subject><subject>RP-HPLC</subject><subject>Scopus</subject><issn>1727-2394</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFjM0KwjAQhHNQUNQ38LAvUIixtvRqVTwoKIhX2TYbGmgTSVfFt1f8u3qZj_kGpiP6k1SlkZpmcU-M2tYWcjZJZJwmsi94vdvksCWuvIYFXan254YcAzoNR6ytRrbegfEB9hd0bI0t38obmFMoKFhHYIJvvrUFfAYj4-vlZ9m6kn2wOBRdg3VLow8HYrxaHvJ1VN1vVJyqeyDUJylVkmUqm_6ZH9fJRuI</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Hemant Shigwan</creator><creator>Arvind Saklani</creator><creator>P. D. 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Under these conditions’ a plot of integrated peak area versus concentration of berberine was found be linear over the concentration range of 0.2 |ig/mL to 150 |ig/mL. The limit of detection was 1ng on column and limit of quantification was 2 ng on column for berberine. The berberine content in B. aristata and B. tinctoria was found to be 3.18% and 1.46% respectively.</abstract><cop>台灣</cop><pub>朝陽科技大學理工學院</pub></addata></record> |
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| identifier | ISSN: 1727-2394 |
| ispartof | International Journal of Applied Science and Engineering, 2013-06, Vol.11 (2), p.203-211 |
| issn | 1727-2394 |
| language | chi |
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| source | EZB Electronic Journals Library |
| subjects | Berberine Photo Diode Array detector RP-HPLC Scopus |
| title | HPLC Method Development and Validation for Quantification of Berberine from Berberis aristata and Berberis tinctoria |
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