Isolation of MDCK cells with low expression of mdr1 gene and their use inmembrane permeability screening

The Madin-Darby canine kidney (MDCK) cell line is frequently used for permeability screening in drug discovery. It contains endogenous transporters, most prominently canine multidrug resistance P-glycoprotein (Mdr1), which can interfere with studies of P-glycoprotein substrate assessment and permeab...

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Veröffentlicht in:	Acta Pharmaceutica 2022-06, Vol.72 (2), p.275
Hauptverfasser:	BOKULIĆ, ANA, PADOVAN, JASNA, DARIJA STUPIN-POLANČEC, DARIJA STUPIN-POLANČEC, MILIĆ, ASTRID
Format:	Artikel
Sprache:	eng
Schlagworte:	clone isolation efflux ratio LC-MS/MSX MDCK P-glycoprotein permeability qRT-PCR
Online-Zugang:	Volltext
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