The effect of erythropoietin on chloride levels during hypoxia reoxygenation injury in rats

Objective. This experimental study examined the effect of erythropoietin (Epo) in a rat model and particularly in a hypoxiareoxygenation (HR) protocol. The effect of that molecule was studied biochemically using blood mean chloride (Cl) levels. Materials and methods. 40 rats of mean weight 247.7 g were used in the study. Cl levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reoxygenation. Erythropoietin was administered only in groups C and D. Results. Epo administration non-significantly decreased Cl levels by 1.07%+0.91% (p=0.2635). Reoxygenation time nonsignificantly decreased Cl levels by 0.68%+0.92% (P= 0.4457). However, erythropoietin administration and reoxygenation time together produced a non-significant combined effect in decreasing Cl levels by 0.74%+0.54% (P= 0.1701). Conclusions. Epo administration, reoxygenation time and their interaction have non-significant, short-term, decreasing effects on Cl levels.