Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population
Aim To study the distribution of allele frequencies of 15 short tandem repeat (STR) loci in a representative sample of Croatian population. Methods A total of 195 unrelated Caucasian individuals born in Croatia, from 14 counties and the City of Zagreb, were sampled for the analysis. All the tested i...
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| Veröffentlicht in: | Croatian medical journal 2007-08, Vol.48 (4.), p.473 |
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| container_title | Croatian medical journal |
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| creator | Projic, Petar Škaro, Vedrana Šamija, Ivana Durmić-Pašić, Adaleta Pojskić, Naris Kovačević, Lejla Bakal, Narcisa Primorac, Dragan Marjanović, Damir |
| description | Aim To study the distribution of allele frequencies of 15 short
tandem repeat (STR) loci in a representative sample of Croatian
population.
Methods A total of 195 unrelated Caucasian individuals born in
Croatia, from 14 counties and the City of Zagreb, were sampled
for the analysis. All the tested individuals were voluntary donors.
Buccal swab was used as the DNA source. AmpFlSTR® Identifiler®
was applied to simultaneously amplify 15 STR loci. Total reaction
volume was 12.5 μL. The PCR amplification was carried out in PE
Gene Amp PCR System Thermal Cycler. Electrophoresis of the
amplification products was preformed on an ABI PRISM 3130
Genetic Analyzer. After PCR amplification and separation by
electrophoresis, raw data were compiled, analyzed, and numerical
allele designations of the profiles were obtained. Deviation from
Hardy-Weinberg equilibrium, observed and expected heterozygosity,
power of discrimination, and power of exclusion were calculated.
Bonferroni’s correction was used before each comparative
analysis.
Results We compared Croatian data with those obtained from
geographically neighboring European populations. The significant
difference (at P |
| format | Article |
| fullrecord | <record><control><sourceid>hrcak</sourceid><recordid>TN_cdi_hrcak_primary_oai_hrcak_srce_hr_16586</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_hrcak_srce_hr_16586</sourcerecordid><originalsourceid>FETCH-hrcak_primary_oai_hrcak_srce_hr_165863</originalsourceid><addsrcrecordid>eNqVjE0KwjAQhYMoWH_uMAewkJo26FJEceFC1H0Z6xSjaVInVfD2VvQCrt73Ph6vI6JEqWk8S7TuikiqTMXzTKZ9MQjhKuVUpqmOxGlhLVmCNdP9Qa4wFKD0DEkGh4vnBo7ozlTBnmrCBra-MGDcpzIFcg025klwwKpuT3wJS_atQgc7Xz9si96NRK9EG2j8y6GYrFfH5Sa-cIG3vGZTIb9yjyb_msAFtZgnOptp9ef8DbPwTuw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Projic, Petar ; Škaro, Vedrana ; Šamija, Ivana ; Durmić-Pašić, Adaleta ; Pojskić, Naris ; Kovačević, Lejla ; Bakal, Narcisa ; Primorac, Dragan ; Marjanović, Damir</creator><creatorcontrib>Projic, Petar ; Škaro, Vedrana ; Šamija, Ivana ; Durmić-Pašić, Adaleta ; Pojskić, Naris ; Kovačević, Lejla ; Bakal, Narcisa ; Primorac, Dragan ; Marjanović, Damir</creatorcontrib><description>Aim To study the distribution of allele frequencies of 15 short
tandem repeat (STR) loci in a representative sample of Croatian
population.
Methods A total of 195 unrelated Caucasian individuals born in
Croatia, from 14 counties and the City of Zagreb, were sampled
for the analysis. All the tested individuals were voluntary donors.
Buccal swab was used as the DNA source. AmpFlSTR® Identifiler®
was applied to simultaneously amplify 15 STR loci. Total reaction
volume was 12.5 μL. The PCR amplification was carried out in PE
Gene Amp PCR System Thermal Cycler. Electrophoresis of the
amplification products was preformed on an ABI PRISM 3130
Genetic Analyzer. After PCR amplification and separation by
electrophoresis, raw data were compiled, analyzed, and numerical
allele designations of the profiles were obtained. Deviation from
Hardy-Weinberg equilibrium, observed and expected heterozygosity,
power of discrimination, and power of exclusion were calculated.
Bonferroni’s correction was used before each comparative
analysis.
Results We compared Croatian data with those obtained from
geographically neighboring European populations. The significant
difference (at P<0.01) in allele frequencies was recorded only between
Croatian and Slovenian populations for vWA locus. There
was no significant deviation from Hardy-Weinberg equilibrium
for all the observed loci.
Conclusion Obtained population data concurred with the expected
“STR data frame” for this part of Europe.</description><identifier>ISSN: 0353-9504</identifier><identifier>EISSN: 1332-8166</identifier><identifier>CODEN: CMEJEN</identifier><language>eng</language><publisher>Medicinski fakultet Sveučilišta u Zagrebu, Medicinski fakultet Sveučilišta u Splitu, Medicinski fakultet Sveučilišta u Rijeci</publisher><subject>Croatian population ; Identifiler ; STR population data</subject><ispartof>Croatian medical journal, 2007-08, Vol.48 (4.), p.473</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,780,784,885</link.rule.ids></links><search><creatorcontrib>Projic, Petar</creatorcontrib><creatorcontrib>Škaro, Vedrana</creatorcontrib><creatorcontrib>Šamija, Ivana</creatorcontrib><creatorcontrib>Durmić-Pašić, Adaleta</creatorcontrib><creatorcontrib>Pojskić, Naris</creatorcontrib><creatorcontrib>Kovačević, Lejla</creatorcontrib><creatorcontrib>Bakal, Narcisa</creatorcontrib><creatorcontrib>Primorac, Dragan</creatorcontrib><creatorcontrib>Marjanović, Damir</creatorcontrib><title>Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population</title><title>Croatian medical journal</title><description>Aim To study the distribution of allele frequencies of 15 short
tandem repeat (STR) loci in a representative sample of Croatian
population.
Methods A total of 195 unrelated Caucasian individuals born in
Croatia, from 14 counties and the City of Zagreb, were sampled
for the analysis. All the tested individuals were voluntary donors.
Buccal swab was used as the DNA source. AmpFlSTR® Identifiler®
was applied to simultaneously amplify 15 STR loci. Total reaction
volume was 12.5 μL. The PCR amplification was carried out in PE
Gene Amp PCR System Thermal Cycler. Electrophoresis of the
amplification products was preformed on an ABI PRISM 3130
Genetic Analyzer. After PCR amplification and separation by
electrophoresis, raw data were compiled, analyzed, and numerical
allele designations of the profiles were obtained. Deviation from
Hardy-Weinberg equilibrium, observed and expected heterozygosity,
power of discrimination, and power of exclusion were calculated.
Bonferroni’s correction was used before each comparative
analysis.
Results We compared Croatian data with those obtained from
geographically neighboring European populations. The significant
difference (at P<0.01) in allele frequencies was recorded only between
Croatian and Slovenian populations for vWA locus. There
was no significant deviation from Hardy-Weinberg equilibrium
for all the observed loci.
Conclusion Obtained population data concurred with the expected
“STR data frame” for this part of Europe.</description><subject>Croatian population</subject><subject>Identifiler</subject><subject>STR population data</subject><issn>0353-9504</issn><issn>1332-8166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqVjE0KwjAQhYMoWH_uMAewkJo26FJEceFC1H0Z6xSjaVInVfD2VvQCrt73Ph6vI6JEqWk8S7TuikiqTMXzTKZ9MQjhKuVUpqmOxGlhLVmCNdP9Qa4wFKD0DEkGh4vnBo7ozlTBnmrCBra-MGDcpzIFcg025klwwKpuT3wJS_atQgc7Xz9si96NRK9EG2j8y6GYrFfH5Sa-cIG3vGZTIb9yjyb_msAFtZgnOptp9ef8DbPwTuw</recordid><startdate>20070815</startdate><enddate>20070815</enddate><creator>Projic, Petar</creator><creator>Škaro, Vedrana</creator><creator>Šamija, Ivana</creator><creator>Durmić-Pašić, Adaleta</creator><creator>Pojskić, Naris</creator><creator>Kovačević, Lejla</creator><creator>Bakal, Narcisa</creator><creator>Primorac, Dragan</creator><creator>Marjanović, Damir</creator><general>Medicinski fakultet Sveučilišta u Zagrebu, Medicinski fakultet Sveučilišta u Splitu, Medicinski fakultet Sveučilišta u Rijeci</general><scope>VP8</scope></search><sort><creationdate>20070815</creationdate><title>Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population</title><author>Projic, Petar ; Škaro, Vedrana ; Šamija, Ivana ; Durmić-Pašić, Adaleta ; Pojskić, Naris ; Kovačević, Lejla ; Bakal, Narcisa ; Primorac, Dragan ; Marjanović, Damir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-hrcak_primary_oai_hrcak_srce_hr_165863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Croatian population</topic><topic>Identifiler</topic><topic>STR population data</topic><toplevel>online_resources</toplevel><creatorcontrib>Projic, Petar</creatorcontrib><creatorcontrib>Škaro, Vedrana</creatorcontrib><creatorcontrib>Šamija, Ivana</creatorcontrib><creatorcontrib>Durmić-Pašić, Adaleta</creatorcontrib><creatorcontrib>Pojskić, Naris</creatorcontrib><creatorcontrib>Kovačević, Lejla</creatorcontrib><creatorcontrib>Bakal, Narcisa</creatorcontrib><creatorcontrib>Primorac, Dragan</creatorcontrib><creatorcontrib>Marjanović, Damir</creatorcontrib><collection>Hrcak: Portal of scientific journals of Croatia</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Projic, Petar</au><au>Škaro, Vedrana</au><au>Šamija, Ivana</au><au>Durmić-Pašić, Adaleta</au><au>Pojskić, Naris</au><au>Kovačević, Lejla</au><au>Bakal, Narcisa</au><au>Primorac, Dragan</au><au>Marjanović, Damir</au><format>book</format><genre>document</genre><ristype>GEN</ristype><atitle>Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population</atitle><jtitle>Croatian medical journal</jtitle><date>2007-08-15</date><risdate>2007</risdate><volume>48</volume><issue>4.</issue><spage>473</spage><pages>473-</pages><issn>0353-9504</issn><eissn>1332-8166</eissn><coden>CMEJEN</coden><abstract>Aim To study the distribution of allele frequencies of 15 short
tandem repeat (STR) loci in a representative sample of Croatian
population.
Methods A total of 195 unrelated Caucasian individuals born in
Croatia, from 14 counties and the City of Zagreb, were sampled
for the analysis. All the tested individuals were voluntary donors.
Buccal swab was used as the DNA source. AmpFlSTR® Identifiler®
was applied to simultaneously amplify 15 STR loci. Total reaction
volume was 12.5 μL. The PCR amplification was carried out in PE
Gene Amp PCR System Thermal Cycler. Electrophoresis of the
amplification products was preformed on an ABI PRISM 3130
Genetic Analyzer. After PCR amplification and separation by
electrophoresis, raw data were compiled, analyzed, and numerical
allele designations of the profiles were obtained. Deviation from
Hardy-Weinberg equilibrium, observed and expected heterozygosity,
power of discrimination, and power of exclusion were calculated.
Bonferroni’s correction was used before each comparative
analysis.
Results We compared Croatian data with those obtained from
geographically neighboring European populations. The significant
difference (at P<0.01) in allele frequencies was recorded only between
Croatian and Slovenian populations for vWA locus. There
was no significant deviation from Hardy-Weinberg equilibrium
for all the observed loci.
Conclusion Obtained population data concurred with the expected
“STR data frame” for this part of Europe.</abstract><pub>Medicinski fakultet Sveučilišta u Zagrebu, Medicinski fakultet Sveučilišta u Splitu, Medicinski fakultet Sveučilišta u Rijeci</pub><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0353-9504 |
| ispartof | Croatian medical journal, 2007-08, Vol.48 (4.), p.473 |
| issn | 0353-9504 1332-8166 |
| language | eng |
| recordid | cdi_hrcak_primary_oai_hrcak_srce_hr_16586 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Croatian population Identifiler STR population data |
| title | Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population |
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