Reusable, Noninvasive, and Sensitive Fluorescence Enhanced ZnO-Nanorod-Based Microarrays for Quantitative Detection of AFP in Human Serum
The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by t...
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| Veröffentlicht in: | BioMed research international 2021, Vol.2021, p.1-11, Article 9916909 |
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| description | The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by the use of ZnO-nanorods and is investigated as a substrate which enhance the florescent signal to diagnose the hepatocellular carcinoma (HCC) at early stages. The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-nanorods enhance the fluorescent signal 7.2 times as compared to the substrate without ZnO-nanorods. The microarray showed sensitivity of 1504.7 ng ml-1 and limit of detection of 0.1 pg ml-1 in wide dynamic range of 0.05 pg-10 μg ml-1 for alpha fetoprotein (AFP) detection in 10% human serum. This immunoassay was successfully applied for human serum samples to detect tumor marker with good recoveries. The ZnO-nanorod substrate is a simple protein microarray which showed a great promise for developing a low-cost, sensitive, and high-throughput protein assay platform for several applications in both fundamental research and clinical diagnosis. |
| doi_str_mv | 10.1155/2021/9916909 |
| format | Article |
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Bryant ; P Bryant Chase</contributor><creatorcontrib>Rafique, Saima ; Kiyani, Farukh ; Jawaid, Sumbal ; Nasir, Rubina ; Ahmad, Mahmoosh ; Bashir, Shazia ; Idress, Muhammad ; Khan, Jan Sher ; Akram, Rizwan ; Chase, P. Bryant ; P Bryant Chase</creatorcontrib><description>The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by the use of ZnO-nanorods and is investigated as a substrate which enhance the florescent signal to diagnose the hepatocellular carcinoma (HCC) at early stages. The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-nanorods enhance the fluorescent signal 7.2 times as compared to the substrate without ZnO-nanorods. The microarray showed sensitivity of 1504.7 ng ml-1 and limit of detection of 0.1 pg ml-1 in wide dynamic range of 0.05 pg-10 μg ml-1 for alpha fetoprotein (AFP) detection in 10% human serum. This immunoassay was successfully applied for human serum samples to detect tumor marker with good recoveries. The ZnO-nanorod substrate is a simple protein microarray which showed a great promise for developing a low-cost, sensitive, and high-throughput protein assay platform for several applications in both fundamental research and clinical diagnosis.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2021/9916909</identifier><identifier>PMID: 34327239</identifier><language>eng</language><publisher>LONDON: Hindawi</publisher><subject>Antibodies ; Antigens ; Arrays ; Biotechnology & Applied Microbiology ; Calibration ; Cancer ; Cancer therapies ; Deposition ; DNA chips ; DNA microarrays ; Fabrication ; Fluorescence ; Glycerol ; Hepatocellular carcinoma ; Immunoassay ; Life Sciences & Biomedicine ; LITAF protein ; Liver cancer ; Medicine, Research & Experimental ; Nanoparticles ; Nanorods ; Protein arrays ; Proteins ; Research & Experimental Medicine ; Science & Technology ; Sensitivity enhancement ; Silicon nitride ; Substrates ; Tumor markers ; Zinc oxide</subject><ispartof>BioMed research international, 2021, Vol.2021, p.1-11, Article 9916909</ispartof><rights>Copyright © 2021 Saima Rafique et al.</rights><rights>Copyright © 2021 Saima Rafique et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 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The sensitivity is enhanced by the use of ZnO-nanorods and is investigated as a substrate which enhance the florescent signal to diagnose the hepatocellular carcinoma (HCC) at early stages. The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-nanorods enhance the fluorescent signal 7.2 times as compared to the substrate without ZnO-nanorods. The microarray showed sensitivity of 1504.7 ng ml-1 and limit of detection of 0.1 pg ml-1 in wide dynamic range of 0.05 pg-10 μg ml-1 for alpha fetoprotein (AFP) detection in 10% human serum. This immunoassay was successfully applied for human serum samples to detect tumor marker with good recoveries. 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The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-nanorods enhance the fluorescent signal 7.2 times as compared to the substrate without ZnO-nanorods. The microarray showed sensitivity of 1504.7 ng ml-1 and limit of detection of 0.1 pg ml-1 in wide dynamic range of 0.05 pg-10 μg ml-1 for alpha fetoprotein (AFP) detection in 10% human serum. This immunoassay was successfully applied for human serum samples to detect tumor marker with good recoveries. 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| source | Wiley-Blackwell Open Access Collection; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Antibodies Antigens Arrays Biotechnology & Applied Microbiology Calibration Cancer Cancer therapies Deposition DNA chips DNA microarrays Fabrication Fluorescence Glycerol Hepatocellular carcinoma Immunoassay Life Sciences & Biomedicine LITAF protein Liver cancer Medicine, Research & Experimental Nanoparticles Nanorods Protein arrays Proteins Research & Experimental Medicine Science & Technology Sensitivity enhancement Silicon nitride Substrates Tumor markers Zinc oxide |
| title | Reusable, Noninvasive, and Sensitive Fluorescence Enhanced ZnO-Nanorod-Based Microarrays for Quantitative Detection of AFP in Human Serum |
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