HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise
1 Exercise Physiology and Metabolism Laboratory, Department of Physiology, and 2 Howard Florey Institute of Experimental Physiology and Medicine, The University of Melbourne, Parkville, Victoria 3052, Australia To examine the effect of exercise on heat shock protein (HSP) 72 mRNA expression in ske...
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| Veröffentlicht in: | Journal of applied physiology (1985) 2000-09, Vol.89 (3), p.1055-1060 |
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| container_title | Journal of applied physiology (1985) |
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| creator | Febbraio, M. A Koukoulas, I |
| description | 1 Exercise Physiology and Metabolism Laboratory, Department
of Physiology, and 2 Howard Florey Institute of Experimental
Physiology and Medicine, The University of Melbourne, Parkville,
Victoria 3052, Australia
To examine the
effect of exercise on heat shock protein (HSP) 72 mRNA expression
in skeletal muscle, five healthy humans (20 ± 1 yr; 64 ± 3 kg; peak O 2 uptake of 2.55 ± 0.2 l/min) cycled until
exhaustion at a workload corresponding to 63% peak O 2
uptake. Muscle was sampled from the vastus lateralis, and
muscle temperature was measured at rest (R), 10 min of exercise
(Min10), ~40 min before fatigue (F-40 = 144 ± 7 min), and
fatigue (F = 186 ± 15 min). Muscle samples were analyzed for
HSP72 mRNA expression, as well as glycogen and lactate concentration.
Muscle temperature increased ( P |
| doi_str_mv | 10.1152/jappl.2000.89.3.1055 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_highw</sourceid><recordid>TN_cdi_highwire_physiology_jap_89_3_1055</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>62966093</sourcerecordid><originalsourceid>FETCH-LOGICAL-c533t-8088f4e6da590bcc348e70338618dd949a416b38b49037160954fe3366757f5f3</originalsourceid><addsrcrecordid>eNqFkVtrFTEUhYMo9rT6D0QGheKDM-Y6SR5LsVYoKFifQ87MnssxczGZaM-_N9NzKCqIT1mQb62dnYXQC4ILQgR9t7Pz7AqKMS6ULlhBsBCP0CZd0ZyUmDxGGyUFzqVQ8gSdhrDDmHAuyFN0QrAWJRN4g9z1l8-SZi2MkMHd7CGEfhqz2U_tvf4Bbp_1Y-XBBghJZV0c7JiFb-BgsS4bYqgcZHX0_diuPjeNLdRvU1pnY1hSQpLgqz7AM_SksS7A8-N5hr5evb-9vM5vPn34eHlxk1eCsSVXWKmGQ1lbofG2qhhXIDFjqiSqrjXXlpNyy9SWa8xk2lUL3gBjZSmFbETDztD5ITc953uEsJihDxU4Z0eYYjCSUkylJv8FiSKs1Eok8NVf4G6KfkxLGEop0YoJmiB-gCo_heChMbPvB-v3hmCzdmbuOzNrZ0Zpw8zaWbK9PGbH7QD1b6ZDSQl4fQRsqKxrvB3Tbz5wmhGhy0S9OVBd33Y_ew9m7vapTDe1-3XwHxP5v9Gr6Nwt3C2r58Fi5rphvwACM8Ek</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>222198352</pqid></control><display><type>article</type><title>HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>American Physiological Society Paid</source><source>Alma/SFX Local Collection</source><creator>Febbraio, M. A ; Koukoulas, I</creator><creatorcontrib>Febbraio, M. A ; Koukoulas, I</creatorcontrib><description>1 Exercise Physiology and Metabolism Laboratory, Department
of Physiology, and 2 Howard Florey Institute of Experimental
Physiology and Medicine, The University of Melbourne, Parkville,
Victoria 3052, Australia
To examine the
effect of exercise on heat shock protein (HSP) 72 mRNA expression
in skeletal muscle, five healthy humans (20 ± 1 yr; 64 ± 3 kg; peak O 2 uptake of 2.55 ± 0.2 l/min) cycled until
exhaustion at a workload corresponding to 63% peak O 2
uptake. Muscle was sampled from the vastus lateralis, and
muscle temperature was measured at rest (R), 10 min of exercise
(Min10), ~40 min before fatigue (F-40 = 144 ± 7 min), and
fatigue (F = 186 ± 15 min). Muscle samples were analyzed for
HSP72 mRNA expression, as well as glycogen and lactate concentration.
Muscle temperature increased ( P < 0.05) during the
first 10 min of exercise but then remained constant for the duration of
the exercise. Similarly, lactate concentration increased
( P < 0.05) when Min10 was compared with R but
decreased ( P < 0.05) thereafter, such that
concentrations at F-40 and F were not different from those at R. In
contrast, muscle glycogen concentration fell progressively throughout
exercise (486 ± 74 vs. 25 ± 7 mmol/kg dry weight for R and
F, respectively; P < 0.05). HSP72 mRNA was
detected at R but did not increase by Min10. However, HSP72 mRNA
increased ( P < 0.05) 2.2 ± 0.5- and 2.6 ± 0.9-fold, respectively, when F-40 and F were compared with R. These
data demonstrate that HSP72 mRNA increases progressively during acute
cycling, suggesting that processes that take place throughout
concentric exercise are capable of initiating a stress response.
contraction; glycogenolysis; real-time polymerase chain reaction; temperature</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.2000.89.3.1055</identifier><identifier>PMID: 10956350</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Adult ; Bicycling ; Biochemistry ; Biological and medical sciences ; Exercise ; Exercise - physiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression - physiology ; Genetics ; Glycogen - metabolism ; Heat-Shock Proteins - genetics ; Homeostasis ; HSP72 Heat-Shock Proteins ; Humans ; Lactic Acid - metabolism ; Muscle, Skeletal - physiology ; Muscular system ; Physical Endurance ; Proteins ; RNA, Messenger - metabolism ; Space life sciences ; Striated muscle. Tendons ; Time Factors ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of applied physiology (1985), 2000-09, Vol.89 (3), p.1055-1060</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright American Physiological Society Sep 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-8088f4e6da590bcc348e70338618dd949a416b38b49037160954fe3366757f5f3</citedby><cites>FETCH-LOGICAL-c533t-8088f4e6da590bcc348e70338618dd949a416b38b49037160954fe3366757f5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=931596$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10956350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Febbraio, M. A</creatorcontrib><creatorcontrib>Koukoulas, I</creatorcontrib><title>HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>1 Exercise Physiology and Metabolism Laboratory, Department
of Physiology, and 2 Howard Florey Institute of Experimental
Physiology and Medicine, The University of Melbourne, Parkville,
Victoria 3052, Australia
To examine the
effect of exercise on heat shock protein (HSP) 72 mRNA expression
in skeletal muscle, five healthy humans (20 ± 1 yr; 64 ± 3 kg; peak O 2 uptake of 2.55 ± 0.2 l/min) cycled until
exhaustion at a workload corresponding to 63% peak O 2
uptake. Muscle was sampled from the vastus lateralis, and
muscle temperature was measured at rest (R), 10 min of exercise
(Min10), ~40 min before fatigue (F-40 = 144 ± 7 min), and
fatigue (F = 186 ± 15 min). Muscle samples were analyzed for
HSP72 mRNA expression, as well as glycogen and lactate concentration.
Muscle temperature increased ( P < 0.05) during the
first 10 min of exercise but then remained constant for the duration of
the exercise. Similarly, lactate concentration increased
( P < 0.05) when Min10 was compared with R but
decreased ( P < 0.05) thereafter, such that
concentrations at F-40 and F were not different from those at R. In
contrast, muscle glycogen concentration fell progressively throughout
exercise (486 ± 74 vs. 25 ± 7 mmol/kg dry weight for R and
F, respectively; P < 0.05). HSP72 mRNA was
detected at R but did not increase by Min10. However, HSP72 mRNA
increased ( P < 0.05) 2.2 ± 0.5- and 2.6 ± 0.9-fold, respectively, when F-40 and F were compared with R. These
data demonstrate that HSP72 mRNA increases progressively during acute
cycling, suggesting that processes that take place throughout
concentric exercise are capable of initiating a stress response.
contraction; glycogenolysis; real-time polymerase chain reaction; temperature</description><subject>Adult</subject><subject>Bicycling</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Exercise</subject><subject>Exercise - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - physiology</subject><subject>Genetics</subject><subject>Glycogen - metabolism</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Homeostasis</subject><subject>HSP72 Heat-Shock Proteins</subject><subject>Humans</subject><subject>Lactic Acid - metabolism</subject><subject>Muscle, Skeletal - physiology</subject><subject>Muscular system</subject><subject>Physical Endurance</subject><subject>Proteins</subject><subject>RNA, Messenger - metabolism</subject><subject>Space life sciences</subject><subject>Striated muscle. Tendons</subject><subject>Time Factors</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtrFTEUhYMo9rT6D0QGheKDM-Y6SR5LsVYoKFifQ87MnssxczGZaM-_N9NzKCqIT1mQb62dnYXQC4ILQgR9t7Pz7AqKMS6ULlhBsBCP0CZd0ZyUmDxGGyUFzqVQ8gSdhrDDmHAuyFN0QrAWJRN4g9z1l8-SZi2MkMHd7CGEfhqz2U_tvf4Bbp_1Y-XBBghJZV0c7JiFb-BgsS4bYqgcZHX0_diuPjeNLdRvU1pnY1hSQpLgqz7AM_SksS7A8-N5hr5evb-9vM5vPn34eHlxk1eCsSVXWKmGQ1lbofG2qhhXIDFjqiSqrjXXlpNyy9SWa8xk2lUL3gBjZSmFbETDztD5ITc953uEsJihDxU4Z0eYYjCSUkylJv8FiSKs1Eok8NVf4G6KfkxLGEop0YoJmiB-gCo_heChMbPvB-v3hmCzdmbuOzNrZ0Zpw8zaWbK9PGbH7QD1b6ZDSQl4fQRsqKxrvB3Tbz5wmhGhy0S9OVBd33Y_ew9m7vapTDe1-3XwHxP5v9Gr6Nwt3C2r58Fi5rphvwACM8Ek</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Febbraio, M. A</creator><creator>Koukoulas, I</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise</title><author>Febbraio, M. A ; Koukoulas, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-8088f4e6da590bcc348e70338618dd949a416b38b49037160954fe3366757f5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Bicycling</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Exercise</topic><topic>Exercise - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - physiology</topic><topic>Genetics</topic><topic>Glycogen - metabolism</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Homeostasis</topic><topic>HSP72 Heat-Shock Proteins</topic><topic>Humans</topic><topic>Lactic Acid - metabolism</topic><topic>Muscle, Skeletal - physiology</topic><topic>Muscular system</topic><topic>Physical Endurance</topic><topic>Proteins</topic><topic>RNA, Messenger - metabolism</topic><topic>Space life sciences</topic><topic>Striated muscle. Tendons</topic><topic>Time Factors</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Febbraio, M. A</creatorcontrib><creatorcontrib>Koukoulas, I</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Febbraio, M. A</au><au>Koukoulas, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>89</volume><issue>3</issue><spage>1055</spage><epage>1060</epage><pages>1055-1060</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>1 Exercise Physiology and Metabolism Laboratory, Department
of Physiology, and 2 Howard Florey Institute of Experimental
Physiology and Medicine, The University of Melbourne, Parkville,
Victoria 3052, Australia
To examine the
effect of exercise on heat shock protein (HSP) 72 mRNA expression
in skeletal muscle, five healthy humans (20 ± 1 yr; 64 ± 3 kg; peak O 2 uptake of 2.55 ± 0.2 l/min) cycled until
exhaustion at a workload corresponding to 63% peak O 2
uptake. Muscle was sampled from the vastus lateralis, and
muscle temperature was measured at rest (R), 10 min of exercise
(Min10), ~40 min before fatigue (F-40 = 144 ± 7 min), and
fatigue (F = 186 ± 15 min). Muscle samples were analyzed for
HSP72 mRNA expression, as well as glycogen and lactate concentration.
Muscle temperature increased ( P < 0.05) during the
first 10 min of exercise but then remained constant for the duration of
the exercise. Similarly, lactate concentration increased
( P < 0.05) when Min10 was compared with R but
decreased ( P < 0.05) thereafter, such that
concentrations at F-40 and F were not different from those at R. In
contrast, muscle glycogen concentration fell progressively throughout
exercise (486 ± 74 vs. 25 ± 7 mmol/kg dry weight for R and
F, respectively; P < 0.05). HSP72 mRNA was
detected at R but did not increase by Min10. However, HSP72 mRNA
increased ( P < 0.05) 2.2 ± 0.5- and 2.6 ± 0.9-fold, respectively, when F-40 and F were compared with R. These
data demonstrate that HSP72 mRNA increases progressively during acute
cycling, suggesting that processes that take place throughout
concentric exercise are capable of initiating a stress response.
contraction; glycogenolysis; real-time polymerase chain reaction; temperature</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>10956350</pmid><doi>10.1152/jappl.2000.89.3.1055</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 8750-7587 |
| ispartof | Journal of applied physiology (1985), 2000-09, Vol.89 (3), p.1055-1060 |
| issn | 8750-7587 1522-1601 |
| language | eng |
| recordid | cdi_highwire_physiology_jap_89_3_1055 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; American Physiological Society Paid; Alma/SFX Local Collection |
| subjects | Adult Bicycling Biochemistry Biological and medical sciences Exercise Exercise - physiology Fundamental and applied biological sciences. Psychology Gene Expression - physiology Genetics Glycogen - metabolism Heat-Shock Proteins - genetics Homeostasis HSP72 Heat-Shock Proteins Humans Lactic Acid - metabolism Muscle, Skeletal - physiology Muscular system Physical Endurance Proteins RNA, Messenger - metabolism Space life sciences Striated muscle. Tendons Time Factors Vertebrates: osteoarticular system, musculoskeletal system |
| title | HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise |
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