Oxytocin as an antidiuretic hormone. II. Role of V2 vasopressin receptor
C. L. Chou, S. R. DiGiovanni, A. Luther, S. J. Lolait and M. A. Knepper Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. We conducted this study to determine what receptor mediates the effect of o...
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| Veröffentlicht in: | American journal of physiology. Renal, fluid and electrolyte physiology fluid and electrolyte physiology, 1995-07, Vol.269 (1), p.78-F85 |
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| container_title | American journal of physiology. Renal, fluid and electrolyte physiology |
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| creator | Chou, C. L DiGiovanni, S. R Luther, A Lolait, S. J Knepper, M. A |
| description | C. L. Chou, S. R. DiGiovanni, A. Luther, S. J. Lolait and M. A. Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
We conducted this study to determine what receptor mediates the effect of
oxytocin to increase osmotic water permeability (Pf) in the rat inner
medullary collecting duct (IMCD). Reverse transcription-polymerase chain
reaction (RT-PCR) experiments demonstrated that mRNA for both the oxytocin
receptor and the V2 receptor is present in the rat terminal IMCD. In
isolated perfused IMCD segments, we found that the V2 vasopressin receptor
antagonist [d(CH2)5(1),D-Ile2,Ile4,Arg8]vasopressin, but not oxytocin
receptor antagonists, blocked the hydrosmotic response to 200 pM oxytocin.
The selective oxytocin receptor agonist [Thr4,Gly7]oxytocin did not
increase water permeability. Oxytocin also increased urea permeability in
IMCD segments. Studies in IMCD suspensions showed that oxytocin increases
adenosine 3',5'-cyclic monophosphate production in a dose-dependent fashion
with a half-maximal (EC50) response at 5.2 nM. The dose-response curves
were virtually identical for IMCD suspensions from Sprague-Dawley rats and
Brattleboro rats. The oxytocin dose-response curve was displaced to the
right of the vasopressin dose-response curve (EC50, 0.44 nM). From these
results, we conclude that the V2 receptor mediates the hydrosmotic action
of oxytocin in rat IMCD. |
| format | Article |
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Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
We conducted this study to determine what receptor mediates the effect of
oxytocin to increase osmotic water permeability (Pf) in the rat inner
medullary collecting duct (IMCD). Reverse transcription-polymerase chain
reaction (RT-PCR) experiments demonstrated that mRNA for both the oxytocin
receptor and the V2 receptor is present in the rat terminal IMCD. In
isolated perfused IMCD segments, we found that the V2 vasopressin receptor
antagonist [d(CH2)5(1),D-Ile2,Ile4,Arg8]vasopressin, but not oxytocin
receptor antagonists, blocked the hydrosmotic response to 200 pM oxytocin.
The selective oxytocin receptor agonist [Thr4,Gly7]oxytocin did not
increase water permeability. Oxytocin also increased urea permeability in
IMCD segments. Studies in IMCD suspensions showed that oxytocin increases
adenosine 3',5'-cyclic monophosphate production in a dose-dependent fashion
with a half-maximal (EC50) response at 5.2 nM. The dose-response curves
were virtually identical for IMCD suspensions from Sprague-Dawley rats and
Brattleboro rats. The oxytocin dose-response curve was displaced to the
right of the vasopressin dose-response curve (EC50, 0.44 nM). From these
results, we conclude that the V2 receptor mediates the hydrosmotic action
of oxytocin in rat IMCD.</description><identifier>ISSN: 0363-6127</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 2161-1157</identifier><identifier>PMID: 7631834</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Base Sequence ; Cyclic AMP - biosynthesis ; Kidney Tubules, Collecting - metabolism ; Male ; Molecular Probes - genetics ; Molecular Sequence Data ; Oxytocin - pharmacology ; Oxytocin - physiology ; Permeability ; Polymerase Chain Reaction ; Rats ; Rats, Brattleboro ; Rats, Sprague-Dawley ; Receptors, Oxytocin - antagonists & inhibitors ; Receptors, Oxytocin - genetics ; Receptors, Vasopressin - physiology ; RNA, Messenger - metabolism ; Transcription, Genetic ; Urea - pharmacokinetics ; Vasopressins - pharmacology ; Vasopressins - physiology ; Water - metabolism</subject><ispartof>American journal of physiology. Renal, fluid and electrolyte physiology, 1995-07, Vol.269 (1), p.78-F85</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7631834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chou, C. L</creatorcontrib><creatorcontrib>DiGiovanni, S. R</creatorcontrib><creatorcontrib>Luther, A</creatorcontrib><creatorcontrib>Lolait, S. J</creatorcontrib><creatorcontrib>Knepper, M. A</creatorcontrib><title>Oxytocin as an antidiuretic hormone. II. Role of V2 vasopressin receptor</title><title>American journal of physiology. Renal, fluid and electrolyte physiology</title><addtitle>Am J Physiol</addtitle><description>C. L. Chou, S. R. DiGiovanni, A. Luther, S. J. Lolait and M. A. Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
We conducted this study to determine what receptor mediates the effect of
oxytocin to increase osmotic water permeability (Pf) in the rat inner
medullary collecting duct (IMCD). Reverse transcription-polymerase chain
reaction (RT-PCR) experiments demonstrated that mRNA for both the oxytocin
receptor and the V2 receptor is present in the rat terminal IMCD. In
isolated perfused IMCD segments, we found that the V2 vasopressin receptor
antagonist [d(CH2)5(1),D-Ile2,Ile4,Arg8]vasopressin, but not oxytocin
receptor antagonists, blocked the hydrosmotic response to 200 pM oxytocin.
The selective oxytocin receptor agonist [Thr4,Gly7]oxytocin did not
increase water permeability. Oxytocin also increased urea permeability in
IMCD segments. Studies in IMCD suspensions showed that oxytocin increases
adenosine 3',5'-cyclic monophosphate production in a dose-dependent fashion
with a half-maximal (EC50) response at 5.2 nM. The dose-response curves
were virtually identical for IMCD suspensions from Sprague-Dawley rats and
Brattleboro rats. The oxytocin dose-response curve was displaced to the
right of the vasopressin dose-response curve (EC50, 0.44 nM). From these
results, we conclude that the V2 receptor mediates the hydrosmotic action
of oxytocin in rat IMCD.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Kidney Tubules, Collecting - metabolism</subject><subject>Male</subject><subject>Molecular Probes - genetics</subject><subject>Molecular Sequence Data</subject><subject>Oxytocin - pharmacology</subject><subject>Oxytocin - physiology</subject><subject>Permeability</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>Rats, Brattleboro</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Oxytocin - antagonists & inhibitors</subject><subject>Receptors, Oxytocin - genetics</subject><subject>Receptors, Vasopressin - physiology</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription, Genetic</subject><subject>Urea - pharmacokinetics</subject><subject>Vasopressins - pharmacology</subject><subject>Vasopressins - physiology</subject><subject>Water - metabolism</subject><issn>0363-6127</issn><issn>0002-9513</issn><issn>2161-1157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotj09LwzAAxYMos04_gpCTt47mT5P0KMO5wmAg6jWkabpmtE1NWrXffoX18t7h_d6DdwMijBiKEUr5LYgSwkjMEOb34CGEc5JgzARbgRVnBAlCI7A__k-D07aDKkA1azfY0o7eDFbD2vnWdWYD83wDP1xjoKvgN4a_KrjemxDmmjfa9IPzj-CuUk0wT4uvwdfu7XO7jw_H93z7eohrlGZDXGJNCEWpylSVZFmpE6UxIpRxXFBENc54gRIhdJHSitOqpCWjtNJMc0yIEGQNXq67vXc_owmDbG3QpmlUZ9wYJOcUi1lm8HkBx6I1pey9bZWf5HJ9zuNrXttT_We9kX09Besad5qkOs__OtVIzDKJ5I4LcgG6y2T_</recordid><startdate>199507</startdate><enddate>199507</enddate><creator>Chou, C. L</creator><creator>DiGiovanni, S. R</creator><creator>Luther, A</creator><creator>Lolait, S. J</creator><creator>Knepper, M. A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199507</creationdate><title>Oxytocin as an antidiuretic hormone. II. Role of V2 vasopressin receptor</title><author>Chou, C. L ; DiGiovanni, S. R ; Luther, A ; Lolait, S. J ; Knepper, M. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h159t-d2c33415a9af099dc0ac2134672b414c297b1088cb54f74fd4d644fc6c7233883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cyclic AMP - biosynthesis</topic><topic>Kidney Tubules, Collecting - metabolism</topic><topic>Male</topic><topic>Molecular Probes - genetics</topic><topic>Molecular Sequence Data</topic><topic>Oxytocin - pharmacology</topic><topic>Oxytocin - physiology</topic><topic>Permeability</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Rats, Brattleboro</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Oxytocin - antagonists & inhibitors</topic><topic>Receptors, Oxytocin - genetics</topic><topic>Receptors, Vasopressin - physiology</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcription, Genetic</topic><topic>Urea - pharmacokinetics</topic><topic>Vasopressins - pharmacology</topic><topic>Vasopressins - physiology</topic><topic>Water - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chou, C. L</creatorcontrib><creatorcontrib>DiGiovanni, S. R</creatorcontrib><creatorcontrib>Luther, A</creatorcontrib><creatorcontrib>Lolait, S. J</creatorcontrib><creatorcontrib>Knepper, M. A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Renal, fluid and electrolyte physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chou, C. L</au><au>DiGiovanni, S. R</au><au>Luther, A</au><au>Lolait, S. J</au><au>Knepper, M. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxytocin as an antidiuretic hormone. II. Role of V2 vasopressin receptor</atitle><jtitle>American journal of physiology. Renal, fluid and electrolyte physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1995-07</date><risdate>1995</risdate><volume>269</volume><issue>1</issue><spage>78</spage><epage>F85</epage><pages>78-F85</pages><issn>0363-6127</issn><issn>0002-9513</issn><eissn>2161-1157</eissn><abstract>C. L. Chou, S. R. DiGiovanni, A. Luther, S. J. Lolait and M. A. Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
We conducted this study to determine what receptor mediates the effect of
oxytocin to increase osmotic water permeability (Pf) in the rat inner
medullary collecting duct (IMCD). Reverse transcription-polymerase chain
reaction (RT-PCR) experiments demonstrated that mRNA for both the oxytocin
receptor and the V2 receptor is present in the rat terminal IMCD. In
isolated perfused IMCD segments, we found that the V2 vasopressin receptor
antagonist [d(CH2)5(1),D-Ile2,Ile4,Arg8]vasopressin, but not oxytocin
receptor antagonists, blocked the hydrosmotic response to 200 pM oxytocin.
The selective oxytocin receptor agonist [Thr4,Gly7]oxytocin did not
increase water permeability. Oxytocin also increased urea permeability in
IMCD segments. Studies in IMCD suspensions showed that oxytocin increases
adenosine 3',5'-cyclic monophosphate production in a dose-dependent fashion
with a half-maximal (EC50) response at 5.2 nM. The dose-response curves
were virtually identical for IMCD suspensions from Sprague-Dawley rats and
Brattleboro rats. The oxytocin dose-response curve was displaced to the
right of the vasopressin dose-response curve (EC50, 0.44 nM). From these
results, we conclude that the V2 receptor mediates the hydrosmotic action
of oxytocin in rat IMCD.</abstract><cop>United States</cop><pmid>7631834</pmid></addata></record> |
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| ispartof | American journal of physiology. Renal, fluid and electrolyte physiology, 1995-07, Vol.269 (1), p.78-F85 |
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| subjects | Animals Base Sequence Cyclic AMP - biosynthesis Kidney Tubules, Collecting - metabolism Male Molecular Probes - genetics Molecular Sequence Data Oxytocin - pharmacology Oxytocin - physiology Permeability Polymerase Chain Reaction Rats Rats, Brattleboro Rats, Sprague-Dawley Receptors, Oxytocin - antagonists & inhibitors Receptors, Oxytocin - genetics Receptors, Vasopressin - physiology RNA, Messenger - metabolism Transcription, Genetic Urea - pharmacokinetics Vasopressins - pharmacology Vasopressins - physiology Water - metabolism |
| title | Oxytocin as an antidiuretic hormone. II. Role of V2 vasopressin receptor |
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