Proximal HCO3- reabsorption and the determinants of tubular and capillary PCO2 in the rat

D. A. Maddox, L. J. Atherton, W. M. Deen and F. J. Gennari Studies were carried out in Munich-Wistar rats to define the CO2 partial pressure (PCO2) profile in the surface tubules and capillaries of the kidney and to relate these measurements to proximal tubular HCO3- reabsorption, renal blood flow,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:American journal of physiology. Renal physiology 1984-07, Vol.247 (1), p.73-F81
Hauptverfasser: Maddox, D. A, Atherton, L. J, Deen, W. M, Gennari, F. J
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page F81
container_issue 1
container_start_page 73
container_title American journal of physiology. Renal physiology
container_volume 247
creator Maddox, D. A
Atherton, L. J
Deen, W. M
Gennari, F. J
description D. A. Maddox, L. J. Atherton, W. M. Deen and F. J. Gennari Studies were carried out in Munich-Wistar rats to define the CO2 partial pressure (PCO2) profile in the surface tubules and capillaries of the kidney and to relate these measurements to proximal tubular HCO3- reabsorption, renal blood flow, and O2 consumption. In euvolemic rats, PCO2 in Bowman's space (BS) was 12.5 mmHg higher than in arterial blood, indicating CO2 addition to the arterial tree as it traverses the cortex. PCO2 further rose by 3.9 mmHg between the efferent arteriole (EA) and the peritubular capillaries (PC) (P less than 0.01) and by 4.9 mmHg between BS and the early proximal tubule (EP) (P less than 0.01). In studies with paired measurements, PCO2 in EP was 1.8 mmHg higher than in the adjacent PC (P less than 0.05). HCO3- reabsorption in EP (first 0.4-1.25 mm) was 579 pmol X min-1 X mm-1 (34.3 +/- 4.6% of the filtered load). By use of a model of facilitated diffusion of CO2 across the cell, the trans-epithelial PCO2 gradient in EP can be accounted for by the CO2 generated from HCO3- reabsorption, assuming an intracellular pH of 7.3. In the vascular compartment, roughly half the rise in PCO2 between the afferent arteriole (estimated to equal BS PCO2) and PC can be accounted for by metabolic CO2 production and half by titration of blood buffers by reabsorbed HCO3-.
doi_str_mv 10.1152/ajprenal.1984.247.1.F73
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_highwire_physiology_ajprenal_247_1_F73</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81145805</sourcerecordid><originalsourceid>FETCH-LOGICAL-c278t-24eeedc42f080433c4adfc08d77f22ff9abff5e9e5b79c7d200dcf6ce4f8610d3</originalsourceid><addsrcrecordid>eNpFkM9PwyAUx4nR6Jz-CUZO3lqB0tIdzeLUxGQ76METofBwmK5UaKP770U39fRCvj_e44PQJSU5pSW7Vm99gE61OZ3VPGdc5DRfiOIATRitaJY84hBNSFEVWUWZOEGnMb4RwlhVV8fouOIFoaScoJdV8J9uo1p8P18WGQ6gmuhDPzjfYdUZPKwBGxggbFynuiFib_EwNmOrwo-uVe_a9Nji1XzJsOt-EkENZ-jIqjbC-X5O0fPi9ml-nz0u7x7mN4-ZZqIeMsYBwGjOLKkJLwrNlbGa1EYIy5i1M9VYW8IMykbMtDCMEKNtpYHbuqLEFFN0tevtg38fIQ5y46KGdFMHfoyyppSXNSmTUeyMOvgYA1jZh_TzsJWUyG-o8heq_IYqE1RJZYKakhf7FWOzAfOX21NMerbT1-51_eECyH69jc63_nX7X_rf9wWpKYbA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81145805</pqid></control><display><type>article</type><title>Proximal HCO3- reabsorption and the determinants of tubular and capillary PCO2 in the rat</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Maddox, D. A ; Atherton, L. J ; Deen, W. M ; Gennari, F. J</creator><creatorcontrib>Maddox, D. A ; Atherton, L. J ; Deen, W. M ; Gennari, F. J</creatorcontrib><description>D. A. Maddox, L. J. Atherton, W. M. Deen and F. J. Gennari Studies were carried out in Munich-Wistar rats to define the CO2 partial pressure (PCO2) profile in the surface tubules and capillaries of the kidney and to relate these measurements to proximal tubular HCO3- reabsorption, renal blood flow, and O2 consumption. In euvolemic rats, PCO2 in Bowman's space (BS) was 12.5 mmHg higher than in arterial blood, indicating CO2 addition to the arterial tree as it traverses the cortex. PCO2 further rose by 3.9 mmHg between the efferent arteriole (EA) and the peritubular capillaries (PC) (P less than 0.01) and by 4.9 mmHg between BS and the early proximal tubule (EP) (P less than 0.01). In studies with paired measurements, PCO2 in EP was 1.8 mmHg higher than in the adjacent PC (P less than 0.05). HCO3- reabsorption in EP (first 0.4-1.25 mm) was 579 pmol X min-1 X mm-1 (34.3 +/- 4.6% of the filtered load). By use of a model of facilitated diffusion of CO2 across the cell, the trans-epithelial PCO2 gradient in EP can be accounted for by the CO2 generated from HCO3- reabsorption, assuming an intracellular pH of 7.3. In the vascular compartment, roughly half the rise in PCO2 between the afferent arteriole (estimated to equal BS PCO2) and PC can be accounted for by metabolic CO2 production and half by titration of blood buffers by reabsorbed HCO3-.</description><identifier>ISSN: 0363-6127</identifier><identifier>ISSN: 0002-9513</identifier><identifier>ISSN: 1931-857X</identifier><identifier>EISSN: 2161-1157</identifier><identifier>EISSN: 1522-1466</identifier><identifier>DOI: 10.1152/ajprenal.1984.247.1.F73</identifier><identifier>PMID: 6430105</identifier><language>eng</language><publisher>United States</publisher><subject>Acid-Base Equilibrium ; Animals ; Bicarbonates - metabolism ; Buffers ; Capillaries - metabolism ; Carbon Dioxide - blood ; Glomerular Filtration Rate ; Hydrogen-Ion Concentration ; Kidney Cortex - metabolism ; Kidney Tubules, Proximal - blood supply ; Kidney Tubules, Proximal - metabolism ; Male ; Microelectrodes ; Oxygen Consumption ; Partial Pressure ; Rats ; Renal Circulation</subject><ispartof>American journal of physiology. Renal physiology, 1984-07, Vol.247 (1), p.73-F81</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c278t-24eeedc42f080433c4adfc08d77f22ff9abff5e9e5b79c7d200dcf6ce4f8610d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6430105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maddox, D. A</creatorcontrib><creatorcontrib>Atherton, L. J</creatorcontrib><creatorcontrib>Deen, W. M</creatorcontrib><creatorcontrib>Gennari, F. J</creatorcontrib><title>Proximal HCO3- reabsorption and the determinants of tubular and capillary PCO2 in the rat</title><title>American journal of physiology. Renal physiology</title><addtitle>Am J Physiol</addtitle><description>D. A. Maddox, L. J. Atherton, W. M. Deen and F. J. Gennari Studies were carried out in Munich-Wistar rats to define the CO2 partial pressure (PCO2) profile in the surface tubules and capillaries of the kidney and to relate these measurements to proximal tubular HCO3- reabsorption, renal blood flow, and O2 consumption. In euvolemic rats, PCO2 in Bowman's space (BS) was 12.5 mmHg higher than in arterial blood, indicating CO2 addition to the arterial tree as it traverses the cortex. PCO2 further rose by 3.9 mmHg between the efferent arteriole (EA) and the peritubular capillaries (PC) (P less than 0.01) and by 4.9 mmHg between BS and the early proximal tubule (EP) (P less than 0.01). In studies with paired measurements, PCO2 in EP was 1.8 mmHg higher than in the adjacent PC (P less than 0.05). HCO3- reabsorption in EP (first 0.4-1.25 mm) was 579 pmol X min-1 X mm-1 (34.3 +/- 4.6% of the filtered load). By use of a model of facilitated diffusion of CO2 across the cell, the trans-epithelial PCO2 gradient in EP can be accounted for by the CO2 generated from HCO3- reabsorption, assuming an intracellular pH of 7.3. In the vascular compartment, roughly half the rise in PCO2 between the afferent arteriole (estimated to equal BS PCO2) and PC can be accounted for by metabolic CO2 production and half by titration of blood buffers by reabsorbed HCO3-.</description><subject>Acid-Base Equilibrium</subject><subject>Animals</subject><subject>Bicarbonates - metabolism</subject><subject>Buffers</subject><subject>Capillaries - metabolism</subject><subject>Carbon Dioxide - blood</subject><subject>Glomerular Filtration Rate</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kidney Cortex - metabolism</subject><subject>Kidney Tubules, Proximal - blood supply</subject><subject>Kidney Tubules, Proximal - metabolism</subject><subject>Male</subject><subject>Microelectrodes</subject><subject>Oxygen Consumption</subject><subject>Partial Pressure</subject><subject>Rats</subject><subject>Renal Circulation</subject><issn>0363-6127</issn><issn>0002-9513</issn><issn>1931-857X</issn><issn>2161-1157</issn><issn>1522-1466</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9PwyAUx4nR6Jz-CUZO3lqB0tIdzeLUxGQ76METofBwmK5UaKP770U39fRCvj_e44PQJSU5pSW7Vm99gE61OZ3VPGdc5DRfiOIATRitaJY84hBNSFEVWUWZOEGnMb4RwlhVV8fouOIFoaScoJdV8J9uo1p8P18WGQ6gmuhDPzjfYdUZPKwBGxggbFynuiFib_EwNmOrwo-uVe_a9Nji1XzJsOt-EkENZ-jIqjbC-X5O0fPi9ml-nz0u7x7mN4-ZZqIeMsYBwGjOLKkJLwrNlbGa1EYIy5i1M9VYW8IMykbMtDCMEKNtpYHbuqLEFFN0tevtg38fIQ5y46KGdFMHfoyyppSXNSmTUeyMOvgYA1jZh_TzsJWUyG-o8heq_IYqE1RJZYKakhf7FWOzAfOX21NMerbT1-51_eECyH69jc63_nX7X_rf9wWpKYbA</recordid><startdate>198407</startdate><enddate>198407</enddate><creator>Maddox, D. A</creator><creator>Atherton, L. J</creator><creator>Deen, W. M</creator><creator>Gennari, F. J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198407</creationdate><title>Proximal HCO3- reabsorption and the determinants of tubular and capillary PCO2 in the rat</title><author>Maddox, D. A ; Atherton, L. J ; Deen, W. M ; Gennari, F. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c278t-24eeedc42f080433c4adfc08d77f22ff9abff5e9e5b79c7d200dcf6ce4f8610d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Acid-Base Equilibrium</topic><topic>Animals</topic><topic>Bicarbonates - metabolism</topic><topic>Buffers</topic><topic>Capillaries - metabolism</topic><topic>Carbon Dioxide - blood</topic><topic>Glomerular Filtration Rate</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kidney Cortex - metabolism</topic><topic>Kidney Tubules, Proximal - blood supply</topic><topic>Kidney Tubules, Proximal - metabolism</topic><topic>Male</topic><topic>Microelectrodes</topic><topic>Oxygen Consumption</topic><topic>Partial Pressure</topic><topic>Rats</topic><topic>Renal Circulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maddox, D. A</creatorcontrib><creatorcontrib>Atherton, L. J</creatorcontrib><creatorcontrib>Deen, W. M</creatorcontrib><creatorcontrib>Gennari, F. J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Renal physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maddox, D. A</au><au>Atherton, L. J</au><au>Deen, W. M</au><au>Gennari, F. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proximal HCO3- reabsorption and the determinants of tubular and capillary PCO2 in the rat</atitle><jtitle>American journal of physiology. Renal physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1984-07</date><risdate>1984</risdate><volume>247</volume><issue>1</issue><spage>73</spage><epage>F81</epage><pages>73-F81</pages><issn>0363-6127</issn><issn>0002-9513</issn><issn>1931-857X</issn><eissn>2161-1157</eissn><eissn>1522-1466</eissn><abstract>D. A. Maddox, L. J. Atherton, W. M. Deen and F. J. Gennari Studies were carried out in Munich-Wistar rats to define the CO2 partial pressure (PCO2) profile in the surface tubules and capillaries of the kidney and to relate these measurements to proximal tubular HCO3- reabsorption, renal blood flow, and O2 consumption. In euvolemic rats, PCO2 in Bowman's space (BS) was 12.5 mmHg higher than in arterial blood, indicating CO2 addition to the arterial tree as it traverses the cortex. PCO2 further rose by 3.9 mmHg between the efferent arteriole (EA) and the peritubular capillaries (PC) (P less than 0.01) and by 4.9 mmHg between BS and the early proximal tubule (EP) (P less than 0.01). In studies with paired measurements, PCO2 in EP was 1.8 mmHg higher than in the adjacent PC (P less than 0.05). HCO3- reabsorption in EP (first 0.4-1.25 mm) was 579 pmol X min-1 X mm-1 (34.3 +/- 4.6% of the filtered load). By use of a model of facilitated diffusion of CO2 across the cell, the trans-epithelial PCO2 gradient in EP can be accounted for by the CO2 generated from HCO3- reabsorption, assuming an intracellular pH of 7.3. In the vascular compartment, roughly half the rise in PCO2 between the afferent arteriole (estimated to equal BS PCO2) and PC can be accounted for by metabolic CO2 production and half by titration of blood buffers by reabsorbed HCO3-.</abstract><cop>United States</cop><pmid>6430105</pmid><doi>10.1152/ajprenal.1984.247.1.F73</doi></addata></record>
fulltext fulltext
identifier ISSN: 0363-6127
ispartof American journal of physiology. Renal physiology, 1984-07, Vol.247 (1), p.73-F81
issn 0363-6127
0002-9513
1931-857X
2161-1157
1522-1466
language eng
recordid cdi_highwire_physiology_ajprenal_247_1_F73
source MEDLINE; Alma/SFX Local Collection
subjects Acid-Base Equilibrium
Animals
Bicarbonates - metabolism
Buffers
Capillaries - metabolism
Carbon Dioxide - blood
Glomerular Filtration Rate
Hydrogen-Ion Concentration
Kidney Cortex - metabolism
Kidney Tubules, Proximal - blood supply
Kidney Tubules, Proximal - metabolism
Male
Microelectrodes
Oxygen Consumption
Partial Pressure
Rats
Renal Circulation
title Proximal HCO3- reabsorption and the determinants of tubular and capillary PCO2 in the rat
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A18%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Proximal%20HCO3-%20reabsorption%20and%20the%20determinants%20of%20tubular%20and%20capillary%20PCO2%20in%20the%20rat&rft.jtitle=American%20journal%20of%20physiology.%20Renal%20physiology&rft.au=Maddox,%20D.%20A&rft.date=1984-07&rft.volume=247&rft.issue=1&rft.spage=73&rft.epage=F81&rft.pages=73-F81&rft.issn=0363-6127&rft.eissn=2161-1157&rft_id=info:doi/10.1152/ajprenal.1984.247.1.F73&rft_dat=%3Cproquest_pubme%3E81145805%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81145805&rft_id=info:pmid/6430105&rfr_iscdi=true