COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat
Departments of 1 Neurology, 2 Anatomy and Neurobiology, and 3 Surgery, University of Vermont College of Medicine, Burlington, Vermont 05405 The purpose of this study was to determine the role of cyclooxygenase-2 (COX-2) and its metabolites in lower urinary tract function after induction of acute...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2003-02, Vol.284 (2), p.574-R585 |
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| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
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| creator | Hu, V. Y Malley, S Dattilio, A Folsom, J. B Zvara, P Vizzard, M. A |
| description | Departments of 1 Neurology, 2 Anatomy
and Neurobiology, and 3 Surgery, University of
Vermont College of Medicine, Burlington, Vermont 05405
The purpose of this
study was to determine the role of cyclooxygenase-2 (COX-2) and its
metabolites in lower urinary tract function after induction of acute (4 h), intermediate (48 h), or chronic (10 day) cyclophosphamide
(CYP)-induced cystitis. Bladders were harvested from euthanized female
rats for analyses. Conscious cystometry was used to assess the effects
of a COX-2-specific inhibitor,
5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl2(5 H )-furanone (DFU, 5 mg/kg sc), a disubstituted furanone, in CYP-induced cystitis. COX-2 mRNA was increased in inflamed bladders after acute (12-fold) and
chronic (9-fold) treatment. COX-2 protein expression in inflamed bladders paralleled COX-2 mRNA expression. Prostaglandin
D 2 -methoxime expression in the bladder was significantly
( P 0.01) increased in acute (3-fold) and chronic
(5.5-fold) cystitis. Prostaglandin E 2 was significantly
( P 0.01) increased (2-fold) in the bladder with
intermediate (1.7-fold) and chronic (2.6-fold) cystitis. COX-2-immunoreactive cell profiles were distributed throughout the
inflamed bladder and coexpressed histamine immunoreactivity. Conscious
cystometry in rats treated with CYP + DFU showed increased micturition intervals 4 and 48 h after CYP treatment and decreased intravesical pressures during filling and micturition compared with
rats treated with CYP + vehicle. These studies suggest an involvement of urinary bladder COX-2 and its metabolites in altered micturition reflexes with CYP-induced cystitis.
inflammation; prostaglandins; 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl-2(5 H )-furanone; mast cells; cystometry |
| doi_str_mv | 10.1152/ajpregu.00465.2002 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_highw</sourceid><recordid>TN_cdi_highwire_physiology_ajpregu_284_2_R574</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>12388444</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-5805b125086948beef1cbf0641a2ac18c59c7b9e394b38046904e05bcc7a19f33</originalsourceid><addsrcrecordid>eNp1kF1LwzAUhoMobk7_gBfSP9CZr3bNpQynwkCQCd6FNE3XjH6EJGXrvzd10115dTic93nhPADcIzhHKMGPYmes2vZzCGmazDGE-AJMwwHHiDJ4CaaQpCROEWITcOPcDoYgoeQaTBAmWUYpnYLd8v0rxpFoi8jYznnRdrqI1CE0O6e7NtJt1Gjpe6v9uBrhq70YXCRKr2wkB1l3puqcqUSjCxXrtuilKsLB-UC4kfeViqzwt-CqFLVTd6c5A5-r583yNV6_v7wtn9axpAnxcZLBJEc4gVnKaJYrVSKZlzClSGAhUSYTJhc5U4TRnGThdQapCoiUC4FYScgM4GOvDA85q0purG6EHTiCfBTHT-L4jzg-igvQwxEyfd6o4oycTIVAfAxUelvttVXcVEMwVHfb4a8QZ5Rj_pEsxjz7P7_q63qjDv4XPHPcFCX5BlMFkmc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat</title><source>MEDLINE</source><source>American Physiological Society Paid</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Hu, V. Y ; Malley, S ; Dattilio, A ; Folsom, J. B ; Zvara, P ; Vizzard, M. A</creator><creatorcontrib>Hu, V. Y ; Malley, S ; Dattilio, A ; Folsom, J. B ; Zvara, P ; Vizzard, M. A</creatorcontrib><description>Departments of 1 Neurology, 2 Anatomy
and Neurobiology, and 3 Surgery, University of
Vermont College of Medicine, Burlington, Vermont 05405
The purpose of this
study was to determine the role of cyclooxygenase-2 (COX-2) and its
metabolites in lower urinary tract function after induction of acute (4 h), intermediate (48 h), or chronic (10 day) cyclophosphamide
(CYP)-induced cystitis. Bladders were harvested from euthanized female
rats for analyses. Conscious cystometry was used to assess the effects
of a COX-2-specific inhibitor,
5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl2(5 H )-furanone (DFU, 5 mg/kg sc), a disubstituted furanone, in CYP-induced cystitis. COX-2 mRNA was increased in inflamed bladders after acute (12-fold) and
chronic (9-fold) treatment. COX-2 protein expression in inflamed bladders paralleled COX-2 mRNA expression. Prostaglandin
D 2 -methoxime expression in the bladder was significantly
( P 0.01) increased in acute (3-fold) and chronic
(5.5-fold) cystitis. Prostaglandin E 2 was significantly
( P 0.01) increased (2-fold) in the bladder with
intermediate (1.7-fold) and chronic (2.6-fold) cystitis. COX-2-immunoreactive cell profiles were distributed throughout the
inflamed bladder and coexpressed histamine immunoreactivity. Conscious
cystometry in rats treated with CYP + DFU showed increased micturition intervals 4 and 48 h after CYP treatment and decreased intravesical pressures during filling and micturition compared with
rats treated with CYP + vehicle. These studies suggest an involvement of urinary bladder COX-2 and its metabolites in altered micturition reflexes with CYP-induced cystitis.
inflammation; prostaglandins; 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl-2(5 H )-furanone; mast cells; cystometry</description><identifier>ISSN: 0363-6119</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.00465.2002</identifier><identifier>PMID: 12388444</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Blotting, Western ; Cyclooxygenase 2 ; Cyclophosphamide - administration & dosage ; Cyclophosphamide - pharmacology ; Cystitis - chemically induced ; Cystitis - enzymology ; Cystitis - metabolism ; Cystitis - physiopathology ; Dinoprostone - metabolism ; Drug Administration Schedule ; Female ; Furans - pharmacology ; Gene Expression Regulation - drug effects ; Immunoenzyme Techniques ; Immunohistochemistry ; Isoenzymes - antagonists & inhibitors ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Prostaglandin D2 - analogs & derivatives ; Prostaglandin D2 - metabolism ; Prostaglandin-Endoperoxide Synthases - genetics ; Prostaglandin-Endoperoxide Synthases - metabolism ; Prostaglandins, Synthetic - metabolism ; Rats ; Rats, Wistar ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Urinary Bladder - drug effects ; Urinary Bladder - enzymology ; Urinary Bladder - metabolism ; Urination - drug effects</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 2003-02, Vol.284 (2), p.574-R585</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-5805b125086948beef1cbf0641a2ac18c59c7b9e394b38046904e05bcc7a19f33</citedby><cites>FETCH-LOGICAL-c453t-5805b125086948beef1cbf0641a2ac18c59c7b9e394b38046904e05bcc7a19f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12388444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, V. Y</creatorcontrib><creatorcontrib>Malley, S</creatorcontrib><creatorcontrib>Dattilio, A</creatorcontrib><creatorcontrib>Folsom, J. B</creatorcontrib><creatorcontrib>Zvara, P</creatorcontrib><creatorcontrib>Vizzard, M. A</creatorcontrib><title>COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><description>Departments of 1 Neurology, 2 Anatomy
and Neurobiology, and 3 Surgery, University of
Vermont College of Medicine, Burlington, Vermont 05405
The purpose of this
study was to determine the role of cyclooxygenase-2 (COX-2) and its
metabolites in lower urinary tract function after induction of acute (4 h), intermediate (48 h), or chronic (10 day) cyclophosphamide
(CYP)-induced cystitis. Bladders were harvested from euthanized female
rats for analyses. Conscious cystometry was used to assess the effects
of a COX-2-specific inhibitor,
5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl2(5 H )-furanone (DFU, 5 mg/kg sc), a disubstituted furanone, in CYP-induced cystitis. COX-2 mRNA was increased in inflamed bladders after acute (12-fold) and
chronic (9-fold) treatment. COX-2 protein expression in inflamed bladders paralleled COX-2 mRNA expression. Prostaglandin
D 2 -methoxime expression in the bladder was significantly
( P 0.01) increased in acute (3-fold) and chronic
(5.5-fold) cystitis. Prostaglandin E 2 was significantly
( P 0.01) increased (2-fold) in the bladder with
intermediate (1.7-fold) and chronic (2.6-fold) cystitis. COX-2-immunoreactive cell profiles were distributed throughout the
inflamed bladder and coexpressed histamine immunoreactivity. Conscious
cystometry in rats treated with CYP + DFU showed increased micturition intervals 4 and 48 h after CYP treatment and decreased intravesical pressures during filling and micturition compared with
rats treated with CYP + vehicle. These studies suggest an involvement of urinary bladder COX-2 and its metabolites in altered micturition reflexes with CYP-induced cystitis.
inflammation; prostaglandins; 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl-2(5 H )-furanone; mast cells; cystometry</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cyclooxygenase 2</subject><subject>Cyclophosphamide - administration & dosage</subject><subject>Cyclophosphamide - pharmacology</subject><subject>Cystitis - chemically induced</subject><subject>Cystitis - enzymology</subject><subject>Cystitis - metabolism</subject><subject>Cystitis - physiopathology</subject><subject>Dinoprostone - metabolism</subject><subject>Drug Administration Schedule</subject><subject>Female</subject><subject>Furans - pharmacology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Immunoenzyme Techniques</subject><subject>Immunohistochemistry</subject><subject>Isoenzymes - antagonists & inhibitors</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Prostaglandin D2 - analogs & derivatives</subject><subject>Prostaglandin D2 - metabolism</subject><subject>Prostaglandin-Endoperoxide Synthases - genetics</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>Prostaglandins, Synthetic - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Urinary Bladder - drug effects</subject><subject>Urinary Bladder - enzymology</subject><subject>Urinary Bladder - metabolism</subject><subject>Urination - drug effects</subject><issn>0363-6119</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1LwzAUhoMobk7_gBfSP9CZr3bNpQynwkCQCd6FNE3XjH6EJGXrvzd10115dTic93nhPADcIzhHKMGPYmes2vZzCGmazDGE-AJMwwHHiDJ4CaaQpCROEWITcOPcDoYgoeQaTBAmWUYpnYLd8v0rxpFoi8jYznnRdrqI1CE0O6e7NtJt1Gjpe6v9uBrhq70YXCRKr2wkB1l3puqcqUSjCxXrtuilKsLB-UC4kfeViqzwt-CqFLVTd6c5A5-r583yNV6_v7wtn9axpAnxcZLBJEc4gVnKaJYrVSKZlzClSGAhUSYTJhc5U4TRnGThdQapCoiUC4FYScgM4GOvDA85q0purG6EHTiCfBTHT-L4jzg-igvQwxEyfd6o4oycTIVAfAxUelvttVXcVEMwVHfb4a8QZ5Rj_pEsxjz7P7_q63qjDv4XPHPcFCX5BlMFkmc</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Hu, V. Y</creator><creator>Malley, S</creator><creator>Dattilio, A</creator><creator>Folsom, J. B</creator><creator>Zvara, P</creator><creator>Vizzard, M. A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20030201</creationdate><title>COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat</title><author>Hu, V. Y ; Malley, S ; Dattilio, A ; Folsom, J. B ; Zvara, P ; Vizzard, M. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-5805b125086948beef1cbf0641a2ac18c59c7b9e394b38046904e05bcc7a19f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cyclooxygenase 2</topic><topic>Cyclophosphamide - administration & dosage</topic><topic>Cyclophosphamide - pharmacology</topic><topic>Cystitis - chemically induced</topic><topic>Cystitis - enzymology</topic><topic>Cystitis - metabolism</topic><topic>Cystitis - physiopathology</topic><topic>Dinoprostone - metabolism</topic><topic>Drug Administration Schedule</topic><topic>Female</topic><topic>Furans - pharmacology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Immunoenzyme Techniques</topic><topic>Immunohistochemistry</topic><topic>Isoenzymes - antagonists & inhibitors</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Prostaglandin D2 - analogs & derivatives</topic><topic>Prostaglandin D2 - metabolism</topic><topic>Prostaglandin-Endoperoxide Synthases - genetics</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>Prostaglandins, Synthetic - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Urinary Bladder - drug effects</topic><topic>Urinary Bladder - enzymology</topic><topic>Urinary Bladder - metabolism</topic><topic>Urination - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, V. Y</creatorcontrib><creatorcontrib>Malley, S</creatorcontrib><creatorcontrib>Dattilio, A</creatorcontrib><creatorcontrib>Folsom, J. B</creatorcontrib><creatorcontrib>Zvara, P</creatorcontrib><creatorcontrib>Vizzard, M. A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, V. Y</au><au>Malley, S</au><au>Dattilio, A</au><au>Folsom, J. B</au><au>Zvara, P</au><au>Vizzard, M. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>284</volume><issue>2</issue><spage>574</spage><epage>R585</epage><pages>574-R585</pages><issn>0363-6119</issn><eissn>1522-1490</eissn><abstract>Departments of 1 Neurology, 2 Anatomy
and Neurobiology, and 3 Surgery, University of
Vermont College of Medicine, Burlington, Vermont 05405
The purpose of this
study was to determine the role of cyclooxygenase-2 (COX-2) and its
metabolites in lower urinary tract function after induction of acute (4 h), intermediate (48 h), or chronic (10 day) cyclophosphamide
(CYP)-induced cystitis. Bladders were harvested from euthanized female
rats for analyses. Conscious cystometry was used to assess the effects
of a COX-2-specific inhibitor,
5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl2(5 H )-furanone (DFU, 5 mg/kg sc), a disubstituted furanone, in CYP-induced cystitis. COX-2 mRNA was increased in inflamed bladders after acute (12-fold) and
chronic (9-fold) treatment. COX-2 protein expression in inflamed bladders paralleled COX-2 mRNA expression. Prostaglandin
D 2 -methoxime expression in the bladder was significantly
( P 0.01) increased in acute (3-fold) and chronic
(5.5-fold) cystitis. Prostaglandin E 2 was significantly
( P 0.01) increased (2-fold) in the bladder with
intermediate (1.7-fold) and chronic (2.6-fold) cystitis. COX-2-immunoreactive cell profiles were distributed throughout the
inflamed bladder and coexpressed histamine immunoreactivity. Conscious
cystometry in rats treated with CYP + DFU showed increased micturition intervals 4 and 48 h after CYP treatment and decreased intravesical pressures during filling and micturition compared with
rats treated with CYP + vehicle. These studies suggest an involvement of urinary bladder COX-2 and its metabolites in altered micturition reflexes with CYP-induced cystitis.
inflammation; prostaglandins; 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl-2(5 H )-furanone; mast cells; cystometry</abstract><cop>United States</cop><pmid>12388444</pmid><doi>10.1152/ajpregu.00465.2002</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6119 |
| ispartof | American journal of physiology. Regulatory, integrative and comparative physiology, 2003-02, Vol.284 (2), p.574-R585 |
| issn | 0363-6119 1522-1490 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpregu_284_2_R574 |
| source | MEDLINE; American Physiological Society Paid; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Blotting, Western Cyclooxygenase 2 Cyclophosphamide - administration & dosage Cyclophosphamide - pharmacology Cystitis - chemically induced Cystitis - enzymology Cystitis - metabolism Cystitis - physiopathology Dinoprostone - metabolism Drug Administration Schedule Female Furans - pharmacology Gene Expression Regulation - drug effects Immunoenzyme Techniques Immunohistochemistry Isoenzymes - antagonists & inhibitors Isoenzymes - genetics Isoenzymes - metabolism Prostaglandin D2 - analogs & derivatives Prostaglandin D2 - metabolism Prostaglandin-Endoperoxide Synthases - genetics Prostaglandin-Endoperoxide Synthases - metabolism Prostaglandins, Synthetic - metabolism Rats Rats, Wistar RNA, Messenger - genetics RNA, Messenger - metabolism Urinary Bladder - drug effects Urinary Bladder - enzymology Urinary Bladder - metabolism Urination - drug effects |
| title | COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat |
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