Superoxide dismutase gene expression in skeletal muscle: fiber-specific adaptation to endurance training
1 Department of Kinesiology and Interdisciplinary Nutritional Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706; and 2 Department of Hygiene, National Defense Medical College, Saitama 359, Japan The effects of endurance training on the enzyme activity, protein content, and mRNA a...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1999-09, Vol.277 (3), p.856-R862 |
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| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
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| creator | Hollander, J Fiebig, R Gore, M Bejma, J Ookawara, T Ohno, H Ji, L. L |
| description | 1 Department of Kinesiology and
Interdisciplinary Nutritional Sciences, University of
Wisconsin-Madison, Madison, Wisconsin 53706; and
2 Department of
Hygiene, National Defense Medical College, Saitama 359, Japan
The effects of endurance training on the
enzyme activity, protein content, and mRNA abundance of Mn and CuZn
superoxide dismutase (SOD) were studied in various phenotypes of rat
skeletal muscle. Female Sprague-Dawley rats were randomly divided into
trained (T, n = 8) and untrained (U,
n = 8) groups. Training, consisting of
treadmill running at 27 m/min and 12% grade for 2 h/day, 5 days/wk for
10 wk, significantly increased citrate synthase activity ( P |
| doi_str_mv | 10.1152/ajpregu.1999.277.3.r856 |
| format | Article |
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Interdisciplinary Nutritional Sciences, University of
Wisconsin-Madison, Madison, Wisconsin 53706; and
2 Department of
Hygiene, National Defense Medical College, Saitama 359, Japan
The effects of endurance training on the
enzyme activity, protein content, and mRNA abundance of Mn and CuZn
superoxide dismutase (SOD) were studied in various phenotypes of rat
skeletal muscle. Female Sprague-Dawley rats were randomly divided into
trained (T, n = 8) and untrained (U,
n = 8) groups. Training, consisting of
treadmill running at 27 m/min and 12% grade for 2 h/day, 5 days/wk for
10 wk, significantly increased citrate synthase activity ( P < 0.01) in the type I (soleus),
type IIa (deep vastus lateralis, DVL), and mixed type II (plantaris)
muscles but not in type IIb (superficial vastus lateralis, SVL) muscle.
Mitochondrial (Mn) SOD activity was elevated by 80%
( P < 0.05) with training in DVL. SVL
and plantaris muscle in T rats showed 54 and 42% higher pooled immunoreactive Mn SOD protein content, respectively, than those in U
rats. However, no change in Mn SOD mRNA level was found in any of the
muscles. CuZn SOD activity, protein content, and mRNA level in general
were not affected by training, except for a 160% increase in pooled
CuZn SOD protein in SVL. Training also significantly increased
glutathione peroxidase and catalase activities
( P < 0.05), but only in DVL muscle.
These data indicate that training adaptations of Mn SOD and other
antioxidant enzymes occur primarily in type IIa fibers, probably as a
result of enhanced free radical generation and modest antioxidant
capacity. Differential training responses of mRNA, enzyme protein, and
activity suggest that separate cellular signals may control pre- and
posttranslational regulation of SOD.
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Interdisciplinary Nutritional Sciences, University of
Wisconsin-Madison, Madison, Wisconsin 53706; and
2 Department of
Hygiene, National Defense Medical College, Saitama 359, Japan
The effects of endurance training on the
enzyme activity, protein content, and mRNA abundance of Mn and CuZn
superoxide dismutase (SOD) were studied in various phenotypes of rat
skeletal muscle. Female Sprague-Dawley rats were randomly divided into
trained (T, n = 8) and untrained (U,
n = 8) groups. Training, consisting of
treadmill running at 27 m/min and 12% grade for 2 h/day, 5 days/wk for
10 wk, significantly increased citrate synthase activity ( P < 0.01) in the type I (soleus),
type IIa (deep vastus lateralis, DVL), and mixed type II (plantaris)
muscles but not in type IIb (superficial vastus lateralis, SVL) muscle.
Mitochondrial (Mn) SOD activity was elevated by 80%
( P < 0.05) with training in DVL. SVL
and plantaris muscle in T rats showed 54 and 42% higher pooled immunoreactive Mn SOD protein content, respectively, than those in U
rats. However, no change in Mn SOD mRNA level was found in any of the
muscles. CuZn SOD activity, protein content, and mRNA level in general
were not affected by training, except for a 160% increase in pooled
CuZn SOD protein in SVL. Training also significantly increased
glutathione peroxidase and catalase activities
( P < 0.05), but only in DVL muscle.
These data indicate that training adaptations of Mn SOD and other
antioxidant enzymes occur primarily in type IIa fibers, probably as a
result of enhanced free radical generation and modest antioxidant
capacity. Differential training responses of mRNA, enzyme protein, and
activity suggest that separate cellular signals may control pre- and
posttranslational regulation of SOD.
antioxidant enzyme</description><subject>Adaptation, Biological</subject><subject>Animals</subject><subject>Female</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Muscle Contraction</subject><subject>Muscle Fibers, Skeletal - physiology</subject><subject>Muscle, Skeletal - physiology</subject><subject>Physical Conditioning, Animal</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Space life sciences</subject><subject>Superoxide Dismutase - physiology</subject><issn>0363-6119</issn><issn>0002-9513</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhq0K1C6FvwA-cUuw46-YG6ooVKqERNuz5TiTrEu-sB1199_j1S4UDlxmDvO870gPQu8oKSkV1Qf7uATo15JqrctKqZKVoRbyDG3ytSoo1-QF2hAmWSEp1RfoVYyPhBDOODtHF5TwmgvCN2h7ty4Q5p1vAbc-jmuyEXAPE2DY5R8x-nnCfsLxBwyQ7IDHNboBPuLONxCKuIDznXfYtnZJNh3oNGOY2jXYyQFOwfrJT_1r9LKzQ4Q3p32JHq4_3199LW6_fbm5-nRbOC5ZKpTkgtpWMSY1sbbWinZCUmWVaB3Nk-muqpQQ3EnWNFA3lMuaMymBCu0qdoneH3uXMP9cISYz-uhgGOwE8xqNItmKZiSD6gi6MMcYoDNL8KMNe0OJOUg2J8nmINlkyYaZ71lyTr49vVibEdq_ckerGSiOwNb32ycfwCzbfRY5zP3-T-s_hfr__PU6DPewS7-DzzmztB37Bf9PoXA</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Hollander, J</creator><creator>Fiebig, R</creator><creator>Gore, M</creator><creator>Bejma, J</creator><creator>Ookawara, T</creator><creator>Ohno, H</creator><creator>Ji, L. L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Superoxide dismutase gene expression in skeletal muscle: fiber-specific adaptation to endurance training</title><author>Hollander, J ; Fiebig, R ; Gore, M ; Bejma, J ; Ookawara, T ; Ohno, H ; Ji, L. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-76451ad733690aa8971f5617a75dc1a7539f227554c63bbe8b14684366e159c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adaptation, Biological</topic><topic>Animals</topic><topic>Female</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Muscle Contraction</topic><topic>Muscle Fibers, Skeletal - physiology</topic><topic>Muscle, Skeletal - physiology</topic><topic>Physical Conditioning, Animal</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Space life sciences</topic><topic>Superoxide Dismutase - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hollander, J</creatorcontrib><creatorcontrib>Fiebig, R</creatorcontrib><creatorcontrib>Gore, M</creatorcontrib><creatorcontrib>Bejma, J</creatorcontrib><creatorcontrib>Ookawara, T</creatorcontrib><creatorcontrib>Ohno, H</creatorcontrib><creatorcontrib>Ji, L. L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hollander, J</au><au>Fiebig, R</au><au>Gore, M</au><au>Bejma, J</au><au>Ookawara, T</au><au>Ohno, H</au><au>Ji, L. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Superoxide dismutase gene expression in skeletal muscle: fiber-specific adaptation to endurance training</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>277</volume><issue>3</issue><spage>856</spage><epage>R862</epage><pages>856-R862</pages><issn>0363-6119</issn><issn>0002-9513</issn><eissn>1522-1490</eissn><abstract>1 Department of Kinesiology and
Interdisciplinary Nutritional Sciences, University of
Wisconsin-Madison, Madison, Wisconsin 53706; and
2 Department of
Hygiene, National Defense Medical College, Saitama 359, Japan
The effects of endurance training on the
enzyme activity, protein content, and mRNA abundance of Mn and CuZn
superoxide dismutase (SOD) were studied in various phenotypes of rat
skeletal muscle. Female Sprague-Dawley rats were randomly divided into
trained (T, n = 8) and untrained (U,
n = 8) groups. Training, consisting of
treadmill running at 27 m/min and 12% grade for 2 h/day, 5 days/wk for
10 wk, significantly increased citrate synthase activity ( P < 0.01) in the type I (soleus),
type IIa (deep vastus lateralis, DVL), and mixed type II (plantaris)
muscles but not in type IIb (superficial vastus lateralis, SVL) muscle.
Mitochondrial (Mn) SOD activity was elevated by 80%
( P < 0.05) with training in DVL. SVL
and plantaris muscle in T rats showed 54 and 42% higher pooled immunoreactive Mn SOD protein content, respectively, than those in U
rats. However, no change in Mn SOD mRNA level was found in any of the
muscles. CuZn SOD activity, protein content, and mRNA level in general
were not affected by training, except for a 160% increase in pooled
CuZn SOD protein in SVL. Training also significantly increased
glutathione peroxidase and catalase activities
( P < 0.05), but only in DVL muscle.
These data indicate that training adaptations of Mn SOD and other
antioxidant enzymes occur primarily in type IIa fibers, probably as a
result of enhanced free radical generation and modest antioxidant
capacity. Differential training responses of mRNA, enzyme protein, and
activity suggest that separate cellular signals may control pre- and
posttranslational regulation of SOD.
antioxidant enzyme</abstract><cop>United States</cop><pmid>10484504</pmid><doi>10.1152/ajpregu.1999.277.3.r856</doi></addata></record> |
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| identifier | ISSN: 0363-6119 |
| ispartof | American journal of physiology. Regulatory, integrative and comparative physiology, 1999-09, Vol.277 (3), p.856-R862 |
| issn | 0363-6119 0002-9513 1522-1490 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpregu_277_3_R856 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adaptation, Biological Animals Female Gene Expression Regulation, Enzymologic - physiology Muscle Contraction Muscle Fibers, Skeletal - physiology Muscle, Skeletal - physiology Physical Conditioning, Animal Rats Rats, Sprague-Dawley Space life sciences Superoxide Dismutase - physiology |
| title | Superoxide dismutase gene expression in skeletal muscle: fiber-specific adaptation to endurance training |
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