Sphingomyelinase has an insulin-like effect on glucose transporter translocation in adipocytes
Department of Physiology and Cell Biology, Albany Medical College, Albany 12208; and Department of Biology, Skidmore College, Saratoga Springs, New York 12866 Rat epididymal adipocytes were incubated with 0, 0.1, and 1 mU sphingomyelinase/ml for 30 or 60 min, and glucose uptake and GLUT-1 and GLUT-4...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1998-05, Vol.274 (5), p.1446-R1453 |
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| container_issue | 5 |
| container_start_page | 1446 |
| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
| container_volume | 274 |
| creator | David, T. S Ortiz, P. A Smith, T. R Turinsky, J |
| description | Department of Physiology and Cell Biology, Albany Medical College,
Albany 12208; and Department of Biology, Skidmore College, Saratoga
Springs, New York 12866
Rat
epididymal adipocytes were incubated with 0, 0.1, and 1 mU
sphingomyelinase/ml for 30 or 60 min, and glucose uptake and GLUT-1 and
GLUT-4 translocation were assessed. Adipocytes exposed to 1 mU
sphingomyelinase/ml exhibited a 173% increase in glucose uptake.
Sphingomyelinase had no effect on the abundance of GLUT-1 in the plasma
membrane of adipocytes. In contrast, 1 mU sphingomyelinase/ml increased
plasma membrane content of GLUT-4 by 120% and produced a simultaneous
decrease in GLUT-4 abundance in the low-density microsomal fraction.
Sphingomyelinase had no effect on tyrosine phosphorylation of either
the insulin receptor -subunit or the insulin receptor substrate-1, a
signaling molecule in the insulin signaling pathway. It is concluded
that the incubation of adipocytes with sphingomyelinase results in
insulin-like translocation of GLUT-4 to the plasma membrane and that
this translocation does not occur via the activation of the initial
components of the insulin signaling pathway.
GLUT-1; GLUT-4; insulin receptor -subunit; insulin receptor
substrate-1; tumor necrosis factor- |
| doi_str_mv | 10.1152/ajpregu.1998.274.5.r1446 |
| format | Article |
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Albany 12208; and Department of Biology, Skidmore College, Saratoga
Springs, New York 12866
Rat
epididymal adipocytes were incubated with 0, 0.1, and 1 mU
sphingomyelinase/ml for 30 or 60 min, and glucose uptake and GLUT-1 and
GLUT-4 translocation were assessed. Adipocytes exposed to 1 mU
sphingomyelinase/ml exhibited a 173% increase in glucose uptake.
Sphingomyelinase had no effect on the abundance of GLUT-1 in the plasma
membrane of adipocytes. In contrast, 1 mU sphingomyelinase/ml increased
plasma membrane content of GLUT-4 by 120% and produced a simultaneous
decrease in GLUT-4 abundance in the low-density microsomal fraction.
Sphingomyelinase had no effect on tyrosine phosphorylation of either
the insulin receptor -subunit or the insulin receptor substrate-1, a
signaling molecule in the insulin signaling pathway. It is concluded
that the incubation of adipocytes with sphingomyelinase results in
insulin-like translocation of GLUT-4 to the plasma membrane and that
this translocation does not occur via the activation of the initial
components of the insulin signaling pathway.
GLUT-1; GLUT-4; insulin receptor -subunit; insulin receptor
substrate-1; tumor necrosis factor-</description><identifier>ISSN: 0363-6119</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.1998.274.5.r1446</identifier><identifier>PMID: 9612413</identifier><language>eng</language><publisher>United States</publisher><subject>Adipocytes - metabolism ; Animals ; Biological Transport - drug effects ; Glucose - metabolism ; Glucose Transporter Type 1 ; Glucose Transporter Type 4 ; Hypoglycemic Agents - pharmacology ; Insulin - pharmacology ; Male ; Monosaccharide Transport Proteins - metabolism ; Muscle Proteins ; Rats ; Rats, Sprague-Dawley ; Sphingomyelin Phosphodiesterase - pharmacology</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 1998-05, Vol.274 (5), p.1446-R1453</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c571t-4ea5bdc4a5d50512e62635c4ab3b0f25e3e5cbde74ad3f7ee864de7e607dc6863</citedby><cites>FETCH-LOGICAL-c571t-4ea5bdc4a5d50512e62635c4ab3b0f25e3e5cbde74ad3f7ee864de7e607dc6863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9612413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>David, T. S</creatorcontrib><creatorcontrib>Ortiz, P. A</creatorcontrib><creatorcontrib>Smith, T. R</creatorcontrib><creatorcontrib>Turinsky, J</creatorcontrib><title>Sphingomyelinase has an insulin-like effect on glucose transporter translocation in adipocytes</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol</addtitle><description>Department of Physiology and Cell Biology, Albany Medical College,
Albany 12208; and Department of Biology, Skidmore College, Saratoga
Springs, New York 12866
Rat
epididymal adipocytes were incubated with 0, 0.1, and 1 mU
sphingomyelinase/ml for 30 or 60 min, and glucose uptake and GLUT-1 and
GLUT-4 translocation were assessed. Adipocytes exposed to 1 mU
sphingomyelinase/ml exhibited a 173% increase in glucose uptake.
Sphingomyelinase had no effect on the abundance of GLUT-1 in the plasma
membrane of adipocytes. In contrast, 1 mU sphingomyelinase/ml increased
plasma membrane content of GLUT-4 by 120% and produced a simultaneous
decrease in GLUT-4 abundance in the low-density microsomal fraction.
Sphingomyelinase had no effect on tyrosine phosphorylation of either
the insulin receptor -subunit or the insulin receptor substrate-1, a
signaling molecule in the insulin signaling pathway. It is concluded
that the incubation of adipocytes with sphingomyelinase results in
insulin-like translocation of GLUT-4 to the plasma membrane and that
this translocation does not occur via the activation of the initial
components of the insulin signaling pathway.
GLUT-1; GLUT-4; insulin receptor -subunit; insulin receptor
substrate-1; tumor necrosis factor-</description><subject>Adipocytes - metabolism</subject><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Glucose - metabolism</subject><subject>Glucose Transporter Type 1</subject><subject>Glucose Transporter Type 4</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Male</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Muscle Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sphingomyelin Phosphodiesterase - pharmacology</subject><issn>0363-6119</issn><issn>0002-9513</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtO3DAUhq2qCIZpH6FSVt0l2PElk-4qVC4SEhKXLZbjnCQGT5zaiSBvj6OZQWxY2cf_xUcfQgnBGSE8P1PPg4d2ykhZbrK8YBnPPGFMfEOrKOcpYSX-jlaYCpoKQsoTdBrCM8aYUUaP0XEpSM4IXaGn-6Ezfeu2M1jTqwBJp0Ki-sT0YYovqTUvkEDTgB4T1yetnbSLrtGrPgzOj-B3d-u0Go1bgomqzeD0PEL4gY4aZQP83J9r9Hjx7-H8Kr25vbw-_3uTal6QMWWgeFVrpnjNMSc5iFxQHueKVrjJOVDguqqhYKqmTQGwESxOIHBRa7ERdI1-73oH7_5PEEa5NUGDtaoHNwVZlCWhOSmjcbMzau9C8NDIwZut8rMkWC5o5R6tXNDKiFZyebegjdFf-z-magv1R3DPMup_dnpn2u7VeJBDNwfjrGtneTFZ-wBv46H-U7Ec6iaGs6_Dh5U-b_MOwcagbQ</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>David, T. S</creator><creator>Ortiz, P. A</creator><creator>Smith, T. R</creator><creator>Turinsky, J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980501</creationdate><title>Sphingomyelinase has an insulin-like effect on glucose transporter translocation in adipocytes</title><author>David, T. S ; Ortiz, P. A ; Smith, T. R ; Turinsky, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c571t-4ea5bdc4a5d50512e62635c4ab3b0f25e3e5cbde74ad3f7ee864de7e607dc6863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adipocytes - metabolism</topic><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Glucose - metabolism</topic><topic>Glucose Transporter Type 1</topic><topic>Glucose Transporter Type 4</topic><topic>Hypoglycemic Agents - pharmacology</topic><topic>Insulin - pharmacology</topic><topic>Male</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Muscle Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sphingomyelin Phosphodiesterase - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>David, T. S</creatorcontrib><creatorcontrib>Ortiz, P. A</creatorcontrib><creatorcontrib>Smith, T. R</creatorcontrib><creatorcontrib>Turinsky, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>David, T. S</au><au>Ortiz, P. A</au><au>Smith, T. R</au><au>Turinsky, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sphingomyelinase has an insulin-like effect on glucose transporter translocation in adipocytes</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>274</volume><issue>5</issue><spage>1446</spage><epage>R1453</epage><pages>1446-R1453</pages><issn>0363-6119</issn><issn>0002-9513</issn><eissn>1522-1490</eissn><abstract>Department of Physiology and Cell Biology, Albany Medical College,
Albany 12208; and Department of Biology, Skidmore College, Saratoga
Springs, New York 12866
Rat
epididymal adipocytes were incubated with 0, 0.1, and 1 mU
sphingomyelinase/ml for 30 or 60 min, and glucose uptake and GLUT-1 and
GLUT-4 translocation were assessed. Adipocytes exposed to 1 mU
sphingomyelinase/ml exhibited a 173% increase in glucose uptake.
Sphingomyelinase had no effect on the abundance of GLUT-1 in the plasma
membrane of adipocytes. In contrast, 1 mU sphingomyelinase/ml increased
plasma membrane content of GLUT-4 by 120% and produced a simultaneous
decrease in GLUT-4 abundance in the low-density microsomal fraction.
Sphingomyelinase had no effect on tyrosine phosphorylation of either
the insulin receptor -subunit or the insulin receptor substrate-1, a
signaling molecule in the insulin signaling pathway. It is concluded
that the incubation of adipocytes with sphingomyelinase results in
insulin-like translocation of GLUT-4 to the plasma membrane and that
this translocation does not occur via the activation of the initial
components of the insulin signaling pathway.
GLUT-1; GLUT-4; insulin receptor -subunit; insulin receptor
substrate-1; tumor necrosis factor-</abstract><cop>United States</cop><pmid>9612413</pmid><doi>10.1152/ajpregu.1998.274.5.r1446</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6119 |
| ispartof | American journal of physiology. Regulatory, integrative and comparative physiology, 1998-05, Vol.274 (5), p.1446-R1453 |
| issn | 0363-6119 0002-9513 1522-1490 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpregu_274_5_R1446 |
| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals |
| subjects | Adipocytes - metabolism Animals Biological Transport - drug effects Glucose - metabolism Glucose Transporter Type 1 Glucose Transporter Type 4 Hypoglycemic Agents - pharmacology Insulin - pharmacology Male Monosaccharide Transport Proteins - metabolism Muscle Proteins Rats Rats, Sprague-Dawley Sphingomyelin Phosphodiesterase - pharmacology |
| title | Sphingomyelinase has an insulin-like effect on glucose transporter translocation in adipocytes |
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