GLUT-4 protein and citrate synthase activity in distally or proximally denervated rat soleus muscle
D. L. Fogt, M. J. Slentz, M. E. Tischler and E. J. Henriksen Department of Biochemistry, University of Arizona College of Medicine, Tucson 85721-0093, USA. The potential role of neurotrophic factors in the decline of glucose transporter (GLUT-4) protein levels and citrate synthase (CS) activity was...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1997-01, Vol.272 (1), p.429-R432 |
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| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
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| creator | Fogt, D. L Slentz, M. J Tischler, M. E Henriksen, E. J |
| description | D. L. Fogt, M. J. Slentz, M. E. Tischler and E. J. Henriksen
Department of Biochemistry, University of Arizona College of Medicine, Tucson 85721-0093, USA.
The potential role of neurotrophic factors in the decline of glucose
transporter (GLUT-4) protein levels and citrate synthase (CS) activity was
studied by comparing distally with proximally denervated juvenile rat
soleus muscle. Severing of the tibial nerve produced distal (long stump) or
proximal (short stump) denervation. GLUT-4 levels and CS activities were
measured at 24-h intervals for up to 96 h after denervation. No differences
were observed in GLUT-4 or CS activity between soleus muscles left with
short or long nerve stumps at any time point. However, within just 24 h,
denervation decreased (P < 0.05). GLUT-4 and CS (67.4 +/- 3.3 and 63.4
+/- 1.7% of innervated control values, respectively). Both parameters
continued to decline up to 96 h (44.4 +/- 3.1 and 48.7 +/- 4.0%,
respectively). There was a significant correlation between the GLUT-4
protein level and CS activity over this 96-h period of denervation (r =
0.653, P < 0.001). A similar response in the 24-h denervated soleus of
adult rats was observed. In contrast, 24-h denervation of red gastrocnemius
(type IIa fibers) left with a long nerve stump resulted in a prevention of
the decline of GLUT-4 and CS seen in red gastrocnemius left with a short
nerve stump in both juvenile and adult animals. These results suggest that
unlike type IIa muscles, the decline in GLUT-4 level and CS activity in
type I soleus muscle after denervation results from a lack of coordinated
electrical activity but likely does not involve a neurotrophic agent. These
results also support the hypothesis that there is coregulation of decreased
expression of GLUT-4 protein and CS activity in this model of reduced
neuromuscular activity. |
| doi_str_mv | 10.1152/ajpregu.1997.272.1.R429 |
| format | Article |
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Department of Biochemistry, University of Arizona College of Medicine, Tucson 85721-0093, USA.
The potential role of neurotrophic factors in the decline of glucose
transporter (GLUT-4) protein levels and citrate synthase (CS) activity was
studied by comparing distally with proximally denervated juvenile rat
soleus muscle. Severing of the tibial nerve produced distal (long stump) or
proximal (short stump) denervation. GLUT-4 levels and CS activities were
measured at 24-h intervals for up to 96 h after denervation. No differences
were observed in GLUT-4 or CS activity between soleus muscles left with
short or long nerve stumps at any time point. However, within just 24 h,
denervation decreased (P < 0.05). GLUT-4 and CS (67.4 +/- 3.3 and 63.4
+/- 1.7% of innervated control values, respectively). Both parameters
continued to decline up to 96 h (44.4 +/- 3.1 and 48.7 +/- 4.0%,
respectively). There was a significant correlation between the GLUT-4
protein level and CS activity over this 96-h period of denervation (r =
0.653, P < 0.001). A similar response in the 24-h denervated soleus of
adult rats was observed. In contrast, 24-h denervation of red gastrocnemius
(type IIa fibers) left with a long nerve stump resulted in a prevention of
the decline of GLUT-4 and CS seen in red gastrocnemius left with a short
nerve stump in both juvenile and adult animals. These results suggest that
unlike type IIa muscles, the decline in GLUT-4 level and CS activity in
type I soleus muscle after denervation results from a lack of coordinated
electrical activity but likely does not involve a neurotrophic agent. These
results also support the hypothesis that there is coregulation of decreased
expression of GLUT-4 protein and CS activity in this model of reduced
neuromuscular activity.</description><identifier>ISSN: 0363-6119</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.1997.272.1.R429</identifier><identifier>PMID: 9039039</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Citrate (si)-Synthase - metabolism ; Denervation ; Female ; Foot ; Glucose Transporter Type 4 ; Monosaccharide Transport Proteins - metabolism ; Muscle Proteins ; Muscle, Skeletal - anatomy & histology ; Muscle, Skeletal - innervation ; Muscle, Skeletal - metabolism ; Nervous System Physiological Phenomena ; Organ Size ; Rats ; Rats, Sprague-Dawley ; Reference Values ; Space life sciences</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 1997-01, Vol.272 (1), p.429-R432</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-71131f331fddfad11078b029a71f6016794a971f0e9160a60864873f2621f79c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3027,27906,27907</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9039039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fogt, D. L</creatorcontrib><creatorcontrib>Slentz, M. J</creatorcontrib><creatorcontrib>Tischler, M. E</creatorcontrib><creatorcontrib>Henriksen, E. J</creatorcontrib><title>GLUT-4 protein and citrate synthase activity in distally or proximally denervated rat soleus muscle</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol</addtitle><description>D. L. Fogt, M. J. Slentz, M. E. Tischler and E. J. Henriksen
Department of Biochemistry, University of Arizona College of Medicine, Tucson 85721-0093, USA.
The potential role of neurotrophic factors in the decline of glucose
transporter (GLUT-4) protein levels and citrate synthase (CS) activity was
studied by comparing distally with proximally denervated juvenile rat
soleus muscle. Severing of the tibial nerve produced distal (long stump) or
proximal (short stump) denervation. GLUT-4 levels and CS activities were
measured at 24-h intervals for up to 96 h after denervation. No differences
were observed in GLUT-4 or CS activity between soleus muscles left with
short or long nerve stumps at any time point. However, within just 24 h,
denervation decreased (P < 0.05). GLUT-4 and CS (67.4 +/- 3.3 and 63.4
+/- 1.7% of innervated control values, respectively). Both parameters
continued to decline up to 96 h (44.4 +/- 3.1 and 48.7 +/- 4.0%,
respectively). There was a significant correlation between the GLUT-4
protein level and CS activity over this 96-h period of denervation (r =
0.653, P < 0.001). A similar response in the 24-h denervated soleus of
adult rats was observed. In contrast, 24-h denervation of red gastrocnemius
(type IIa fibers) left with a long nerve stump resulted in a prevention of
the decline of GLUT-4 and CS seen in red gastrocnemius left with a short
nerve stump in both juvenile and adult animals. These results suggest that
unlike type IIa muscles, the decline in GLUT-4 level and CS activity in
type I soleus muscle after denervation results from a lack of coordinated
electrical activity but likely does not involve a neurotrophic agent. These
results also support the hypothesis that there is coregulation of decreased
expression of GLUT-4 protein and CS activity in this model of reduced
neuromuscular activity.</description><subject>Animals</subject><subject>Citrate (si)-Synthase - metabolism</subject><subject>Denervation</subject><subject>Female</subject><subject>Foot</subject><subject>Glucose Transporter Type 4</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Muscle Proteins</subject><subject>Muscle, Skeletal - anatomy & histology</subject><subject>Muscle, Skeletal - innervation</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Nervous System Physiological Phenomena</subject><subject>Organ Size</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Values</subject><subject>Space life sciences</subject><issn>0363-6119</issn><issn>0002-9513</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkNFOwyAYhYnRzDl9BCNX3rXylwrl0iw6TZaYmO2asJZuLF1bgap9e6lrFhMIkHO-AxyE7oDEAI_Jg9q3Vm-7GITgccKTGOKPNBFnaBrUJIJUkHM0JZTRiAGIS3Tl3J4QktKUTtBEEDqMKcoXy_UqSnFrG69NjVVd4Nx4q7zGrq_9TjmNVe7Nl_E9DobCOK-qqseNHaAfc_g7FbrW9itQBQ4sdk2lO4cPncsrfY0uSlU5fTOuM7R-eV7NX6Pl--Jt_rSMcpoyH3EACiUNsyhKVQAQnm1IIhSHkhFgXKRKhD3RAhhRjGQszTgtE5ZAyUVOZ-j-mBve9dlp5-XBuFxXlap10znJs4zSQAQjPxpz2zhndSlbG_5hewlEDvXKsV451CtDvRLkUG8gb8crus1BFydu7DPo0VHfme3u21gt213vTFM12_4U-i_vFy9AiR8</recordid><startdate>19970101</startdate><enddate>19970101</enddate><creator>Fogt, D. L</creator><creator>Slentz, M. J</creator><creator>Tischler, M. E</creator><creator>Henriksen, E. J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970101</creationdate><title>GLUT-4 protein and citrate synthase activity in distally or proximally denervated rat soleus muscle</title><author>Fogt, D. L ; Slentz, M. J ; Tischler, M. E ; Henriksen, E. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-71131f331fddfad11078b029a71f6016794a971f0e9160a60864873f2621f79c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Citrate (si)-Synthase - metabolism</topic><topic>Denervation</topic><topic>Female</topic><topic>Foot</topic><topic>Glucose Transporter Type 4</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Muscle Proteins</topic><topic>Muscle, Skeletal - anatomy & histology</topic><topic>Muscle, Skeletal - innervation</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Nervous System Physiological Phenomena</topic><topic>Organ Size</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Values</topic><topic>Space life sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fogt, D. L</creatorcontrib><creatorcontrib>Slentz, M. J</creatorcontrib><creatorcontrib>Tischler, M. E</creatorcontrib><creatorcontrib>Henriksen, E. J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fogt, D. L</au><au>Slentz, M. J</au><au>Tischler, M. E</au><au>Henriksen, E. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GLUT-4 protein and citrate synthase activity in distally or proximally denervated rat soleus muscle</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1997-01-01</date><risdate>1997</risdate><volume>272</volume><issue>1</issue><spage>429</spage><epage>R432</epage><pages>429-R432</pages><issn>0363-6119</issn><issn>0002-9513</issn><eissn>1522-1490</eissn><abstract>D. L. Fogt, M. J. Slentz, M. E. Tischler and E. J. Henriksen
Department of Biochemistry, University of Arizona College of Medicine, Tucson 85721-0093, USA.
The potential role of neurotrophic factors in the decline of glucose
transporter (GLUT-4) protein levels and citrate synthase (CS) activity was
studied by comparing distally with proximally denervated juvenile rat
soleus muscle. Severing of the tibial nerve produced distal (long stump) or
proximal (short stump) denervation. GLUT-4 levels and CS activities were
measured at 24-h intervals for up to 96 h after denervation. No differences
were observed in GLUT-4 or CS activity between soleus muscles left with
short or long nerve stumps at any time point. However, within just 24 h,
denervation decreased (P < 0.05). GLUT-4 and CS (67.4 +/- 3.3 and 63.4
+/- 1.7% of innervated control values, respectively). Both parameters
continued to decline up to 96 h (44.4 +/- 3.1 and 48.7 +/- 4.0%,
respectively). There was a significant correlation between the GLUT-4
protein level and CS activity over this 96-h period of denervation (r =
0.653, P < 0.001). A similar response in the 24-h denervated soleus of
adult rats was observed. In contrast, 24-h denervation of red gastrocnemius
(type IIa fibers) left with a long nerve stump resulted in a prevention of
the decline of GLUT-4 and CS seen in red gastrocnemius left with a short
nerve stump in both juvenile and adult animals. These results suggest that
unlike type IIa muscles, the decline in GLUT-4 level and CS activity in
type I soleus muscle after denervation results from a lack of coordinated
electrical activity but likely does not involve a neurotrophic agent. These
results also support the hypothesis that there is coregulation of decreased
expression of GLUT-4 protein and CS activity in this model of reduced
neuromuscular activity.</abstract><cop>United States</cop><pmid>9039039</pmid><doi>10.1152/ajpregu.1997.272.1.R429</doi></addata></record> |
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| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Citrate (si)-Synthase - metabolism Denervation Female Foot Glucose Transporter Type 4 Monosaccharide Transport Proteins - metabolism Muscle Proteins Muscle, Skeletal - anatomy & histology Muscle, Skeletal - innervation Muscle, Skeletal - metabolism Nervous System Physiological Phenomena Organ Size Rats Rats, Sprague-Dawley Reference Values Space life sciences |
| title | GLUT-4 protein and citrate synthase activity in distally or proximally denervated rat soleus muscle |
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