Molecular activity of sodium pumps in endotherms and ectotherms
P. L. Else, D. J. Windmill and V. Markus Department of Biomedical Science, University of Wollongong, New South Wales, Australia. Previous research has shown ectotherms to have markedly lower sodium pump metabolism than endotherms. Direct measurement of enzymatic activity of the sodium pump (Na(+)-K(...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1996-11, Vol.271 (5), p.1287-R1294 |
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| container_end_page | R1294 |
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| container_issue | 5 |
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| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
| container_volume | 271 |
| creator | Else, P. L Windmill, D. J Markus, V |
| description | P. L. Else, D. J. Windmill and V. Markus
Department of Biomedical Science, University of Wollongong, New South Wales, Australia.
Previous research has shown ectotherms to have markedly lower sodium pump
metabolism than endotherms. Direct measurement of enzymatic activity of the
sodium pump (Na(+)-K(+)-adenosinetriphosphatase) confirmed this difference.
To determine the source of this difference, sodium pump density was
measured with the use of [3H]ouabain binding. Ectotherms and endotherms
were found to share similar sodium pump numbers. Approximate densities (in
pmol/g) were 250 for skeletal muscle, 500 for liver, 900 for heart, and
8,000 for kidney and brain. Therefore, differences in sodium pump activity
between endotherms and ectotherms were due to differences in turnover rates
or molecular activities of sodium pumps. Molecular activities of sodium
pumps (in ATP/min) of tissues from endotherms were between 6,000 and 12,000
and, for ectotherms, between 1,500 and 2,500. Exceptions were found that
included the heart of Bufo marinus. In a single invertebrate species
studied, Charax destructor, the sodium pumps of the heart had a low
molecular activity characteristic of ectothermic tissues. These results
suggest that during the evolution of endothermy there was a general
increase in the molecular activity of the sodium pump. |
| doi_str_mv | 10.1152/ajpregu.1996.271.5.r1287 |
| format | Article |
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Department of Biomedical Science, University of Wollongong, New South Wales, Australia.
Previous research has shown ectotherms to have markedly lower sodium pump
metabolism than endotherms. Direct measurement of enzymatic activity of the
sodium pump (Na(+)-K(+)-adenosinetriphosphatase) confirmed this difference.
To determine the source of this difference, sodium pump density was
measured with the use of [3H]ouabain binding. Ectotherms and endotherms
were found to share similar sodium pump numbers. Approximate densities (in
pmol/g) were 250 for skeletal muscle, 500 for liver, 900 for heart, and
8,000 for kidney and brain. Therefore, differences in sodium pump activity
between endotherms and ectotherms were due to differences in turnover rates
or molecular activities of sodium pumps. Molecular activities of sodium
pumps (in ATP/min) of tissues from endotherms were between 6,000 and 12,000
and, for ectotherms, between 1,500 and 2,500. Exceptions were found that
included the heart of Bufo marinus. In a single invertebrate species
studied, Charax destructor, the sodium pumps of the heart had a low
molecular activity characteristic of ectothermic tissues. These results
suggest that during the evolution of endothermy there was a general
increase in the molecular activity of the sodium pump.</description><identifier>ISSN: 0363-6119</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.1996.271.5.r1287</identifier><identifier>PMID: 8945966</identifier><language>eng</language><publisher>United States</publisher><subject>Amphibians ; Animals ; Astacoidea ; Body Temperature Regulation ; Fishes ; Gerbillinae ; Guinea Pigs ; Kidney - metabolism ; Liver - metabolism ; Male ; Muscle, Skeletal - metabolism ; Myocardium - metabolism ; Ouabain - metabolism ; Rabbits ; Rats ; Reptiles ; Sodium-Potassium-Exchanging ATPase - metabolism</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 1996-11, Vol.271 (5), p.1287-R1294</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-ce4a4629f837be76dae6b1fca8cf48afb10a38bcc98d0b99ccddeb4482982a483</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8945966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Else, P. L</creatorcontrib><creatorcontrib>Windmill, D. J</creatorcontrib><creatorcontrib>Markus, V</creatorcontrib><title>Molecular activity of sodium pumps in endotherms and ectotherms</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol</addtitle><description>P. L. Else, D. J. Windmill and V. Markus
Department of Biomedical Science, University of Wollongong, New South Wales, Australia.
Previous research has shown ectotherms to have markedly lower sodium pump
metabolism than endotherms. Direct measurement of enzymatic activity of the
sodium pump (Na(+)-K(+)-adenosinetriphosphatase) confirmed this difference.
To determine the source of this difference, sodium pump density was
measured with the use of [3H]ouabain binding. Ectotherms and endotherms
were found to share similar sodium pump numbers. Approximate densities (in
pmol/g) were 250 for skeletal muscle, 500 for liver, 900 for heart, and
8,000 for kidney and brain. Therefore, differences in sodium pump activity
between endotherms and ectotherms were due to differences in turnover rates
or molecular activities of sodium pumps. Molecular activities of sodium
pumps (in ATP/min) of tissues from endotherms were between 6,000 and 12,000
and, for ectotherms, between 1,500 and 2,500. Exceptions were found that
included the heart of Bufo marinus. In a single invertebrate species
studied, Charax destructor, the sodium pumps of the heart had a low
molecular activity characteristic of ectothermic tissues. These results
suggest that during the evolution of endothermy there was a general
increase in the molecular activity of the sodium pump.</description><subject>Amphibians</subject><subject>Animals</subject><subject>Astacoidea</subject><subject>Body Temperature Regulation</subject><subject>Fishes</subject><subject>Gerbillinae</subject><subject>Guinea Pigs</subject><subject>Kidney - metabolism</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Myocardium - metabolism</subject><subject>Ouabain - metabolism</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Reptiles</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><issn>0363-6119</issn><issn>0002-9513</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkFtLwzAYhoMoc05_gpAr71pzaptciYgnmAii1yFN0jWjXWrSKv33dqwMrz5e3sMHDwAQoxTjjNyqbRfsZkixEHlKCpxmacCEFydgOdkkwUygU7BENKdJjrE4BxcxbhFCjDK6AAsuWCbyfAnu3nxj9dCoAJXu3Y_rR-grGL1xQwu7oe0idDtod8b3tQ1thGpnoNX9LC_BWaWaaK_muwJfT4-fDy_J-v359eF-nWjKRJ9oyxTLiag4LUpb5EbZvMSVVlxXjKuqxEhRXmotuEGlEFobY0vGOBGcKMbpCtwcdrvgvwcbe9m6qG3TqJ31Q5QFzwqWEToF-SGog48x2Ep2wbUqjBIjuWcnZ3Zyz05O7GQmP_bspur1_GMoW2uOxRnW5KcHv3ab-tcFK7t6jM43fjMeV_8P_gESi3-C</recordid><startdate>19961101</startdate><enddate>19961101</enddate><creator>Else, P. L</creator><creator>Windmill, D. J</creator><creator>Markus, V</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961101</creationdate><title>Molecular activity of sodium pumps in endotherms and ectotherms</title><author>Else, P. L ; Windmill, D. J ; Markus, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-ce4a4629f837be76dae6b1fca8cf48afb10a38bcc98d0b99ccddeb4482982a483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amphibians</topic><topic>Animals</topic><topic>Astacoidea</topic><topic>Body Temperature Regulation</topic><topic>Fishes</topic><topic>Gerbillinae</topic><topic>Guinea Pigs</topic><topic>Kidney - metabolism</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Myocardium - metabolism</topic><topic>Ouabain - metabolism</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Reptiles</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Else, P. L</creatorcontrib><creatorcontrib>Windmill, D. J</creatorcontrib><creatorcontrib>Markus, V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Else, P. L</au><au>Windmill, D. J</au><au>Markus, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular activity of sodium pumps in endotherms and ectotherms</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1996-11-01</date><risdate>1996</risdate><volume>271</volume><issue>5</issue><spage>1287</spage><epage>R1294</epage><pages>1287-R1294</pages><issn>0363-6119</issn><issn>0002-9513</issn><eissn>1522-1490</eissn><abstract>P. L. Else, D. J. Windmill and V. Markus
Department of Biomedical Science, University of Wollongong, New South Wales, Australia.
Previous research has shown ectotherms to have markedly lower sodium pump
metabolism than endotherms. Direct measurement of enzymatic activity of the
sodium pump (Na(+)-K(+)-adenosinetriphosphatase) confirmed this difference.
To determine the source of this difference, sodium pump density was
measured with the use of [3H]ouabain binding. Ectotherms and endotherms
were found to share similar sodium pump numbers. Approximate densities (in
pmol/g) were 250 for skeletal muscle, 500 for liver, 900 for heart, and
8,000 for kidney and brain. Therefore, differences in sodium pump activity
between endotherms and ectotherms were due to differences in turnover rates
or molecular activities of sodium pumps. Molecular activities of sodium
pumps (in ATP/min) of tissues from endotherms were between 6,000 and 12,000
and, for ectotherms, between 1,500 and 2,500. Exceptions were found that
included the heart of Bufo marinus. In a single invertebrate species
studied, Charax destructor, the sodium pumps of the heart had a low
molecular activity characteristic of ectothermic tissues. These results
suggest that during the evolution of endothermy there was a general
increase in the molecular activity of the sodium pump.</abstract><cop>United States</cop><pmid>8945966</pmid><doi>10.1152/ajpregu.1996.271.5.r1287</doi></addata></record> |
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| identifier | ISSN: 0363-6119 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amphibians Animals Astacoidea Body Temperature Regulation Fishes Gerbillinae Guinea Pigs Kidney - metabolism Liver - metabolism Male Muscle, Skeletal - metabolism Myocardium - metabolism Ouabain - metabolism Rabbits Rats Reptiles Sodium-Potassium-Exchanging ATPase - metabolism |
| title | Molecular activity of sodium pumps in endotherms and ectotherms |
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