Corticosterone decreases nonshivering thermogenesis and increases lipid storage in brown adipose tissue
A. M. Strack, M. J. Bradbury and M. F. Dallman Department of Physiology, University of California, San Francisco 94143-0444. Brown adipose tissue (BAT) contains glucocorticoid receptors; glucocorticoids are required for maintaining differentiated BAT in culture. These studies were performed to deter...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1995-01, Vol.268 (1), p.183-R191 |
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| creator | Strack, A. M Bradbury, M. J Dallman, M. F |
| description | A. M. Strack, M. J. Bradbury and M. F. Dallman
Department of Physiology, University of California, San Francisco 94143-0444.
Brown adipose tissue (BAT) contains glucocorticoid receptors;
glucocorticoids are required for maintaining differentiated BAT in culture.
These studies were performed to determine the effects of corticosterone on
BAT thermogenic function and lipid storage. Rats were adrenalectomized and
given subcutaneous corticosterone pellets in concentrations that maintained
plasma corticosterone constant across the range of 0-20 micrograms/dl or
were sham adrenalectomized. All variables were examined 5 days after
surgery and corticosterone replacement. Measures of BAT
function-thermogenic capacity [guanosine 5'-diphosphate (GDP) binding and
uncoupling protein (UCP; a BAT-specific thermogenic protein)] and storage
(BAT wet wt, protein, and DNA levels) were made. Plasma hormones
(corticosterone, adrenocorticotropic hormone, insulin,
3,3',5-triiodothyronine, and thyroxine were measured. Corticosterone
significantly affected BAT thermogenic measures: UCP content and binding of
GDP to BAT mitochondria decreased with increasing corticosterone; GDP
binding characteristics in BAT from similarly prepared rats examined by
Scatchard analysis showed that maximum binding (Bmax) and dissociation
constant (Kd) decreased with increasing corticosterone dose. BAT DNA was
increased by adrenalectomy and maintained at intact levels with all doses
of corticosterone; BAT lipid storage increased dramatically at
corticosterone values higher than the daily mean level in intact rats.
Histologically, the number and size of lipid droplets within BAT adipocytes
increased markedly with increased corticosterone. White adipose depots were
more sensitive to circulating corticosterone concentrations than were BAT
depots and increased in weight at levels of corticosterone that were at or
below the daily mean level of intact rats. We conclude that, within its
diurnal range of concentration corticosterone acts to inhibit nonshivering
thermogenesis and increase lipid storage. |
| doi_str_mv | 10.1152/ajpregu.1995.268.1.r183 |
| format | Article |
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Department of Physiology, University of California, San Francisco 94143-0444.
Brown adipose tissue (BAT) contains glucocorticoid receptors;
glucocorticoids are required for maintaining differentiated BAT in culture.
These studies were performed to determine the effects of corticosterone on
BAT thermogenic function and lipid storage. Rats were adrenalectomized and
given subcutaneous corticosterone pellets in concentrations that maintained
plasma corticosterone constant across the range of 0-20 micrograms/dl or
were sham adrenalectomized. All variables were examined 5 days after
surgery and corticosterone replacement. Measures of BAT
function-thermogenic capacity [guanosine 5'-diphosphate (GDP) binding and
uncoupling protein (UCP; a BAT-specific thermogenic protein)] and storage
(BAT wet wt, protein, and DNA levels) were made. Plasma hormones
(corticosterone, adrenocorticotropic hormone, insulin,
3,3',5-triiodothyronine, and thyroxine were measured. Corticosterone
significantly affected BAT thermogenic measures: UCP content and binding of
GDP to BAT mitochondria decreased with increasing corticosterone; GDP
binding characteristics in BAT from similarly prepared rats examined by
Scatchard analysis showed that maximum binding (Bmax) and dissociation
constant (Kd) decreased with increasing corticosterone dose. BAT DNA was
increased by adrenalectomy and maintained at intact levels with all doses
of corticosterone; BAT lipid storage increased dramatically at
corticosterone values higher than the daily mean level in intact rats.
Histologically, the number and size of lipid droplets within BAT adipocytes
increased markedly with increased corticosterone. White adipose depots were
more sensitive to circulating corticosterone concentrations than were BAT
depots and increased in weight at levels of corticosterone that were at or
below the daily mean level of intact rats. We conclude that, within its
diurnal range of concentration corticosterone acts to inhibit nonshivering
thermogenesis and increase lipid storage.</description><identifier>ISSN: 0363-6119</identifier><identifier>ISSN: 0002-9513</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.1995.268.1.r183</identifier><identifier>PMID: 7840319</identifier><language>eng</language><publisher>United States</publisher><subject>Adipocytes - cytology ; Adipocytes - drug effects ; Adipocytes - metabolism ; Adipose Tissue - drug effects ; Adipose Tissue - metabolism ; Adipose Tissue, Brown - drug effects ; Adipose Tissue, Brown - metabolism ; Adipose Tissue, Brown - physiology ; Adrenalectomy ; Adrenocorticotropic Hormone - blood ; Adrenocorticotropic Hormone - secretion ; Animals ; Body Temperature Regulation - drug effects ; Body Weight - drug effects ; Carrier Proteins - metabolism ; Corticosterone - administration & dosage ; Corticosterone - blood ; Corticosterone - pharmacology ; DNA - metabolism ; Dose-Response Relationship, Drug ; Drug Implants ; Epididymis ; Glycogen - metabolism ; Guanosine Diphosphate - metabolism ; In Vitro Techniques ; Insulin - blood ; Ion Channels ; Lipid Metabolism ; Male ; Membrane Proteins - metabolism ; Mitochondrial Proteins ; Pituitary Gland, Anterior - drug effects ; Pituitary Gland, Anterior - physiology ; Rats ; Rats, Sprague-Dawley ; Space life sciences ; Thyroxine - blood ; Time Factors ; Triiodothyronine - blood ; Uncoupling Protein 1</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 1995-01, Vol.268 (1), p.183-R191</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-f5da46edeb3bfc2fcf90d62974d4be9954e094dd3e0fdfeabd03bc9ade626c473</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7840319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Strack, A. M</creatorcontrib><creatorcontrib>Bradbury, M. J</creatorcontrib><creatorcontrib>Dallman, M. F</creatorcontrib><title>Corticosterone decreases nonshivering thermogenesis and increases lipid storage in brown adipose tissue</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol</addtitle><description>A. M. Strack, M. J. Bradbury and M. F. Dallman
Department of Physiology, University of California, San Francisco 94143-0444.
Brown adipose tissue (BAT) contains glucocorticoid receptors;
glucocorticoids are required for maintaining differentiated BAT in culture.
These studies were performed to determine the effects of corticosterone on
BAT thermogenic function and lipid storage. Rats were adrenalectomized and
given subcutaneous corticosterone pellets in concentrations that maintained
plasma corticosterone constant across the range of 0-20 micrograms/dl or
were sham adrenalectomized. All variables were examined 5 days after
surgery and corticosterone replacement. Measures of BAT
function-thermogenic capacity [guanosine 5'-diphosphate (GDP) binding and
uncoupling protein (UCP; a BAT-specific thermogenic protein)] and storage
(BAT wet wt, protein, and DNA levels) were made. Plasma hormones
(corticosterone, adrenocorticotropic hormone, insulin,
3,3',5-triiodothyronine, and thyroxine were measured. Corticosterone
significantly affected BAT thermogenic measures: UCP content and binding of
GDP to BAT mitochondria decreased with increasing corticosterone; GDP
binding characteristics in BAT from similarly prepared rats examined by
Scatchard analysis showed that maximum binding (Bmax) and dissociation
constant (Kd) decreased with increasing corticosterone dose. BAT DNA was
increased by adrenalectomy and maintained at intact levels with all doses
of corticosterone; BAT lipid storage increased dramatically at
corticosterone values higher than the daily mean level in intact rats.
Histologically, the number and size of lipid droplets within BAT adipocytes
increased markedly with increased corticosterone. White adipose depots were
more sensitive to circulating corticosterone concentrations than were BAT
depots and increased in weight at levels of corticosterone that were at or
below the daily mean level of intact rats. We conclude that, within its
diurnal range of concentration corticosterone acts to inhibit nonshivering
thermogenesis and increase lipid storage.</description><subject>Adipocytes - cytology</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Adipose Tissue - drug effects</subject><subject>Adipose Tissue - metabolism</subject><subject>Adipose Tissue, Brown - drug effects</subject><subject>Adipose Tissue, Brown - metabolism</subject><subject>Adipose Tissue, Brown - physiology</subject><subject>Adrenalectomy</subject><subject>Adrenocorticotropic Hormone - blood</subject><subject>Adrenocorticotropic Hormone - secretion</subject><subject>Animals</subject><subject>Body Temperature Regulation - drug effects</subject><subject>Body Weight - drug effects</subject><subject>Carrier Proteins - metabolism</subject><subject>Corticosterone - administration & dosage</subject><subject>Corticosterone - blood</subject><subject>Corticosterone - pharmacology</subject><subject>DNA - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Implants</subject><subject>Epididymis</subject><subject>Glycogen - metabolism</subject><subject>Guanosine Diphosphate - metabolism</subject><subject>In Vitro Techniques</subject><subject>Insulin - blood</subject><subject>Ion Channels</subject><subject>Lipid Metabolism</subject><subject>Male</subject><subject>Membrane Proteins - metabolism</subject><subject>Mitochondrial Proteins</subject><subject>Pituitary Gland, Anterior - drug effects</subject><subject>Pituitary Gland, Anterior - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Space life sciences</subject><subject>Thyroxine - blood</subject><subject>Time Factors</subject><subject>Triiodothyronine - blood</subject><subject>Uncoupling Protein 1</subject><issn>0363-6119</issn><issn>0002-9513</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE2L2zAQhsXSJZum_QnL6tSbXclS7OhYQtstBBZK9yxkaWwrOJKrsRvy7-uQbNjTwLwfwzyEPHGWc74uvpr9kKCdcq7UOi_KTc7zxDfijixntci4VOwDWTJRiqzkXD2Qj4h7xpgUUizIotpIJrhaknYb0-htxBFSDEAd2AQGAWmIATv_D5IPLR07SIfYQgD0SE1w1Ic3Y-8H7yiOMZkW5j2tUzwGapwfIgIdPeIEn8h9Y3qEz9e5Iq8_vv_ZPme7l5-_tt92mRWyHLNm7YwswUEt6sYWjW0Uc2WhKulkDfOvEpiSzglgjWvA1I6J2irjoCxKKyuxIl8uvUOKfyfAUR88Wuh7EyBOqKuKF2ojxGysLkabImKCRg_JH0w6ac70GbG-ItZnxHpGrLn-PSOek4_XE1N9AHfLXZnOenbRO992R59AD90Jfexje7qVvuv7D3vijzY</recordid><startdate>19950101</startdate><enddate>19950101</enddate><creator>Strack, A. M</creator><creator>Bradbury, M. J</creator><creator>Dallman, M. F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950101</creationdate><title>Corticosterone decreases nonshivering thermogenesis and increases lipid storage in brown adipose tissue</title><author>Strack, A. M ; Bradbury, M. J ; Dallman, M. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-f5da46edeb3bfc2fcf90d62974d4be9954e094dd3e0fdfeabd03bc9ade626c473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adipocytes - cytology</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>Adipose Tissue - drug effects</topic><topic>Adipose Tissue - metabolism</topic><topic>Adipose Tissue, Brown - drug effects</topic><topic>Adipose Tissue, Brown - metabolism</topic><topic>Adipose Tissue, Brown - physiology</topic><topic>Adrenalectomy</topic><topic>Adrenocorticotropic Hormone - blood</topic><topic>Adrenocorticotropic Hormone - secretion</topic><topic>Animals</topic><topic>Body Temperature Regulation - drug effects</topic><topic>Body Weight - drug effects</topic><topic>Carrier Proteins - metabolism</topic><topic>Corticosterone - administration & dosage</topic><topic>Corticosterone - blood</topic><topic>Corticosterone - pharmacology</topic><topic>DNA - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Implants</topic><topic>Epididymis</topic><topic>Glycogen - metabolism</topic><topic>Guanosine Diphosphate - metabolism</topic><topic>In Vitro Techniques</topic><topic>Insulin - blood</topic><topic>Ion Channels</topic><topic>Lipid Metabolism</topic><topic>Male</topic><topic>Membrane Proteins - metabolism</topic><topic>Mitochondrial Proteins</topic><topic>Pituitary Gland, Anterior - drug effects</topic><topic>Pituitary Gland, Anterior - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Space life sciences</topic><topic>Thyroxine - blood</topic><topic>Time Factors</topic><topic>Triiodothyronine - blood</topic><topic>Uncoupling Protein 1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strack, A. M</creatorcontrib><creatorcontrib>Bradbury, M. J</creatorcontrib><creatorcontrib>Dallman, M. F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strack, A. M</au><au>Bradbury, M. J</au><au>Dallman, M. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corticosterone decreases nonshivering thermogenesis and increases lipid storage in brown adipose tissue</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol</addtitle><date>1995-01-01</date><risdate>1995</risdate><volume>268</volume><issue>1</issue><spage>183</spage><epage>R191</epage><pages>183-R191</pages><issn>0363-6119</issn><issn>0002-9513</issn><eissn>1522-1490</eissn><abstract>A. M. Strack, M. J. Bradbury and M. F. Dallman
Department of Physiology, University of California, San Francisco 94143-0444.
Brown adipose tissue (BAT) contains glucocorticoid receptors;
glucocorticoids are required for maintaining differentiated BAT in culture.
These studies were performed to determine the effects of corticosterone on
BAT thermogenic function and lipid storage. Rats were adrenalectomized and
given subcutaneous corticosterone pellets in concentrations that maintained
plasma corticosterone constant across the range of 0-20 micrograms/dl or
were sham adrenalectomized. All variables were examined 5 days after
surgery and corticosterone replacement. Measures of BAT
function-thermogenic capacity [guanosine 5'-diphosphate (GDP) binding and
uncoupling protein (UCP; a BAT-specific thermogenic protein)] and storage
(BAT wet wt, protein, and DNA levels) were made. Plasma hormones
(corticosterone, adrenocorticotropic hormone, insulin,
3,3',5-triiodothyronine, and thyroxine were measured. Corticosterone
significantly affected BAT thermogenic measures: UCP content and binding of
GDP to BAT mitochondria decreased with increasing corticosterone; GDP
binding characteristics in BAT from similarly prepared rats examined by
Scatchard analysis showed that maximum binding (Bmax) and dissociation
constant (Kd) decreased with increasing corticosterone dose. BAT DNA was
increased by adrenalectomy and maintained at intact levels with all doses
of corticosterone; BAT lipid storage increased dramatically at
corticosterone values higher than the daily mean level in intact rats.
Histologically, the number and size of lipid droplets within BAT adipocytes
increased markedly with increased corticosterone. White adipose depots were
more sensitive to circulating corticosterone concentrations than were BAT
depots and increased in weight at levels of corticosterone that were at or
below the daily mean level of intact rats. We conclude that, within its
diurnal range of concentration corticosterone acts to inhibit nonshivering
thermogenesis and increase lipid storage.</abstract><cop>United States</cop><pmid>7840319</pmid><doi>10.1152/ajpregu.1995.268.1.r183</doi></addata></record> |
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| ispartof | American journal of physiology. Regulatory, integrative and comparative physiology, 1995-01, Vol.268 (1), p.183-R191 |
| issn | 0363-6119 0002-9513 1522-1490 |
| language | eng |
| recordid | cdi_highwire_physiology_ajpregu_268_1_R183 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adipocytes - cytology Adipocytes - drug effects Adipocytes - metabolism Adipose Tissue - drug effects Adipose Tissue - metabolism Adipose Tissue, Brown - drug effects Adipose Tissue, Brown - metabolism Adipose Tissue, Brown - physiology Adrenalectomy Adrenocorticotropic Hormone - blood Adrenocorticotropic Hormone - secretion Animals Body Temperature Regulation - drug effects Body Weight - drug effects Carrier Proteins - metabolism Corticosterone - administration & dosage Corticosterone - blood Corticosterone - pharmacology DNA - metabolism Dose-Response Relationship, Drug Drug Implants Epididymis Glycogen - metabolism Guanosine Diphosphate - metabolism In Vitro Techniques Insulin - blood Ion Channels Lipid Metabolism Male Membrane Proteins - metabolism Mitochondrial Proteins Pituitary Gland, Anterior - drug effects Pituitary Gland, Anterior - physiology Rats Rats, Sprague-Dawley Space life sciences Thyroxine - blood Time Factors Triiodothyronine - blood Uncoupling Protein 1 |
| title | Corticosterone decreases nonshivering thermogenesis and increases lipid storage in brown adipose tissue |
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